pQL126

LOCUS       pQL126             6385 bp DNA     circular SYN 01-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6385
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     primer_bind     186..207
                     /label=olyl419
     misc_feature    235..818
                     /label=P_CMV
                     /label=nonstandard type: promoter_const
     misc_feature    235..614
                     /label=P_CMV_enhancer
                     /label=nonstandard type: promoter_element
     misc_feature    615..818
                     /label=P_CMV_IE
                     /label=nonstandard type: promoter_element
     primer_bind     769..789
                     /label=oMHS-CMV-F
     misc_feature    829
                     /label=putative transcriptional start
     promoter        863..882
                     /label=T7 promoter
     primer_bind     complement(871..894)
                     /label=olyl418
     primer_bind     881..921
                     /label=QL315 ESE-1-F
     primer_bind     881..882
                     /label=T7
     misc_feature    901..1947
                     /label=ESE-1
     primer_bind     1039..1060
                     /label=QL253
     primer_bind     complement(1146..1167)
                     /label=QL254
     primer_bind     1349..1370
                     
     primer_bind     complement(1452..1473)
                     /label=QL260
     primer_bind     1579..1600
                     /label=QL255
     misc_feature    1617..1637
                     /label=shRNA
     primer_bind     complement(1662..1683)
                     /label=QL256
     misc_feature    1847..1867
                     /label=shRNA
     primer_bind     complement(1927..1967)
                     /label=QL316 ESE-1-R
     terminator      1982..2209
                     /label=bGH polyA
     rep_origin      2255..2683
                     /label=f1 bacteriophage origin of replication
                     /label=f1 bacteriophage origin of replication; arrow _f1 
                     ori
     misc_feature    2697..3026
                     /label=P_SV40
                     /label=nonstandard type: promoter_const
     rep_origin      2877..3012
                     /label=SV40 ORI
     misc_feature    2929..2978
                     /label=P_SV40 TSS-region
     CDS             3093..3887
                     /label=Translation 2136-2930
     misc_feature    3093..3884
                     /label=Kan/neoR
                     /label=nonstandard type: resistance
     primer_bind     complement(4227..4247)
                     /label=M13-rev
     misc_binding    complement(4253..4275)
                     /label=LacO
     promoter        complement(4279..4309)
                     /label=lac promoter
     rep_origin      complement(4633..5218)
                     /direction=LEFT
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin o
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     _ori
     CDS             complement(5389..6249)
                     /label=Translation 5292-4432
     misc_feature    complement(5392..6249)
                     /label=AmpR
                     /label=nonstandard type: resistance
     promoter        complement(6250..6354)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 atggctgcaa cctgtgagat tagcaacatt tttagcaact acttcagtgc gatgtacagc
      961 tcggaggact ccaccctggc ctctgttccc cctgctgcca cctttggggc cgatgacttg
     1021 gtactgaccc tgagcaaccc ccagatgtca ttggagggta cagagaaggc cagctggttg
     1081 ggggaacagc cccagttctg gtcgaagacg caggttctgg actggatcag ctaccaagtg
     1141 gagaagaaca agtacgacgc aagcgccatt gacttctcac gatgtgacat ggatggcgcc
     1201 accctctgca attgtgccct tgaggagctg cgtctggtct ttgggcctct gggggaccaa
     1261 ctccatgccc agctgcgaga cctcacttcc agctcttctg atgagctcag ttggatcatt
     1321 gagctgctgg agaaggatgg catggccttc caggaggccc tagacccagg gccctttgac
     1381 cagggcagcc cctttgccca ggagctgctg gacgacgtct ccaccgcagg gactggtgct
     1441 tctcggagct cccactcctc agactccggt ggaagtgacg tggacctgga tcccactgat
     1501 ggcaagctct tccccagcga tggttttcgt gactgcaaga agggggatcc caagcacggg
     1561 aagcggaaac gaggccggcc ccgaaagctg agcaaagagt actgggactg tctcgagggc
     1621 aagaagagca agcacgcgcc cagaggcacc cacctgtggg agttcatccg ggacatcctc
     1681 atccacccgg agctcaacga gggcctcatg aagtgggaga atcggcatga aggcgtcttc
     1741 aagttcctgc gctccgaggc tgtggcccaa ctatggggcc aaaagaaaaa gaacagcaac
     1801 atgacctacg agaagctgag ccgggccatg aggtactact acaaacggga gatcctggaa
     1861 cgggtggatg gccggcgact cgtctacaag tttggcaaaa actcaagcgg ctggaaggag
     1921 gaagaggttc tccagagtcg gaactgatct agagggcccg tttaaacccg ctgatcagcc
     1981 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     2041 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     2101 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     2161 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     2221 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     2281 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2341 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2401 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     2461 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2521 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     2581 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     2641 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     2701 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2761 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     2821 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     2881 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     2941 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     3001 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     3061 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     3121 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     3181 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     3241 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     3301 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     3361 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     3421 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     3481 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     3541 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     3601 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     3661 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     3721 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     3781 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     3841 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     3901 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     3961 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     4021 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     4081 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4141 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     4201 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     4261 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     4321 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     4381 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     4441 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     4501 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     4561 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     4621 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     4681 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     4741 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     4801 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     4861 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4921 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4981 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     5041 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     5101 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     5161 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5221 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5281 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5341 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5401 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5461 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5521 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5581 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5641 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5701 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5761 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5821 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5881 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5941 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6001 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6061 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6121 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6181 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6241 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6301 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6361 tccccgaaaa gtgccacctg acgtc
//