| 质粒名称: | pAAVLINK-EF1a-5'-TECTA |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001711 |
| 产品用途: | 5' AAVLINK plasmid for TECTA expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TECTA |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-5'-TECTA
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-5'-TECTA载体质粒基本信息
pAAVLINK-EF1a-5'-TECTA质粒图谱载体图谱和pAAVLINK-EF1a-5'-TECTA载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-TECTA质粒载体简介
pAAVLINK-EF1a-5'-TECTA质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-TECTA 6778 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6778)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 3, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6778
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 713..3918
/codon_start=1
/label=TECTA
/translation="MNYSSFLRIWVSFIFALVQHQAQPRELMYPFWQNDTKTPKVDDGS
SSEIKLAIPVFFFGVPYRTVYVNNNGVVSFNVLVSQFTPESFPLTDGRAFVAPFWADVH
NGIRGEIYYRETMEPAILKRATKDIRKYFKDMATFSATWVFIVTWEEVTFYGGSSTTPV
NTFQAVLVSDGSYTFTLFNYYEINWTTGTASGGDPLTGLGGVMAQAGFNGGNLTNFFSL
PGSRTPEIVNIQETTNVNVPGRWAFKVDGKEIDPANGCTSRGQFLRRGEVFWDDLNCTV
KCRCLDFNNEIYCQEASCSPYEVCEPKGKFFYCSAVETSTCVVFGEPHYHTFDGFLFHF
QGSCAYLLARQCLQTSSLPFFSVEAKNEHRRGSAVSWVKELSVEVNGYKILIPKGSYGR
VKVNDLVTSLPVTLDLGTVKIYQSGISTAVETDFGLLVTFDGQHYASISVPGSYINSTC
GLCGNYNKNPLDDFLRPDGRPAMSVLDLGESWRVYHADWKCDSGCVDNCTQCDAATEAL
YFGSDYCGFLNKTDGPLWECGTVVDPTAFVHSCVYDLCSVRDNGTLLCQAIQAYALVCQ
ALGIPIGDWRTQTGCVSTVQCPSFSHYSVCTSSCPDTCSDLTASRNCATPCTEGCECNQ
GFVLSTSQCVPLHKCGCDFDGHYYTMGEFFWATANCTVQCLCEEGGDVYCFNKTCGSGE
VCAVEDGYQGCFPKRETVCLLSQNQVLHTFDGASYAFPSEFSYTLLKTCPERPEYLEID
INKKKPDAGPAWLRGLRILVADQEVKIGGIGASEVKLNGQEVELPFFHPSGKLEIYRNK
NSTTVESKGVVTVQYSDIGLLYIRLSTTYFNCTGGLCGFYNANASDEFCLPNGKCTDNL
AVFLESWTTFEEICNGECGDLLKACNNDSELLKFYRSRSRCGIINDPSNSSFLECHGVV
NATAYYRTCLFRLCQSGGNESELCDSVARYASACKNADVEVGPWRTYDFCPLECPENSH
FEECITCTETCETLTLGPICVDSCSEGCQCDEGYALLGSQCVTRSECGCNFEGHQLATN
ETFWVDLDCQIFCYCSGTDN"
misc_feature 1596..1601
/label=SDS
misc_feature 2745..2750
/label=SDS
misc_feature 2892..2897
/label=SDS
misc_feature 2926..2931
/label=SDS
misc_feature 3039..3044
/label=SDS
misc_feature 3052..3057
/label=SDS
intron 3919..3959
/label=Splicing Donor
misc_feature 3963..3996
/label=lox2272-JT1571
repeat_region 4041..4181
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4041..4170
/label=AAV2 ITR
rep_origin 4256..4711
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4993..5097
/gene="bla"
/label=AmpR promoter
CDS 5098..5958
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6129..6717
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgaatta
721 ttcatcattc cttagaattt gggtctcttt catcttcgca cttgtacagc accaagctca
781 gcccagggag ctcatgtatc cattttggca gaatgacacc aaaaccccta aagtagatga
841 tggaagctca tctgagatta agttggccat cccagttttc ttctttggcg ttccttaccg
901 cactgtctat gtcaataaca acggagttgt ttccttcaat gtgctagtga gccagttcac
961 gccagaatcc tttcccctga cagatgggag agccttcgtc gccccatttt gggcagatgt
1021 gcacaatgga attcgaggcg agatctatta cagagagacc atggagcctg ccatcttgaa
1081 aagagccacc aaggacatca ggaagtactt caaagacatg gcaaccttct ctgccacttg
1141 ggttttcatt gtgacatggg aggaagtcac gttttatgga ggcagcagca ccacacctgt
1201 gaacaccttc caggccgtcc tagtgtccga tggctcctat acattcaccc tcttcaatta
1261 ttacgaaatc aactggacca cggggacggc gagtggcggc gaccccctga caggtcttgg
1321 tggagtgatg gcacaggcag gatttaatgg tggaaacctc accaatttct tcagcctccc
1381 ggggtcaaga acccccgaga tcgtgaatat ccaggagacc acaaacgtca atgttccagg
1441 ccgctgggca tttaaagttg atggaaagga aattgaccca gccaatggct gcacctcaag
1501 gggacaattc cttcggcgag gggaggtgtt ttgggatgac ttgaactgca ccgtcaagtg
1561 ccgctgtctg gatttcaaca atgagatcta ctgccaggag gcttcctgta gcccctacga
1621 ggtgtgcgaa cccaaaggca aattcttcta ctgcagcgct gtggagacca gcacatgcgt
1681 ggtgtttggg gagccacact accacacttt tgacggcttc ctcttccact tccaaggctc
1741 ctgtgcctac ttgctggccc gacagtgttt gcagacttcc agcctccctt tcttcagtgt
1801 ggaggccaag aatgaacacc gcagaggttc agccgtctcc tgggtgaagg agctctcagt
1861 ggaggtgaat ggctacaaga ttctcatccc caaaggaagc tatggaagag tcaaggttaa
1921 tgacctagtg acttctttgc ctgtcacctt agacttgggg acagtgaaaa tctaccagag
1981 tggcatatct actgccgtgg aaacagattt tgggctctta gtgacttttg atggccagca
2041 ctacgcctcc atttccgtcc caggctccta tataaactcc acctgtggac tctgtggaaa
2101 ctataataaa aacccactgg atgacttcct ccgcccggat ggcaggccgg ccatgtctgt
2161 cctggatctg ggagagagct ggcgtgtgta ccacgcagac tggaagtgcg actccggctg
2221 cgtcgacaac tgcacccagt gcgacgctgc cactgaagcc ctctactttg gctctgacta
2281 ctgcggcttc ctcaacaaga cagacggccc tctgtgggag tgtggcactg tcgtggaccc
2341 cactgctttt gtgcacagct gcgtgtatga cctgtgcagt gtgagggaca atggcacgct
2401 cctctgccaa gccatccagg cctatgctct tgtgtgccaa gcccttggca ttccaattgg
2461 agactggcga acccagactg ggtgtgtgtc cacagtgcag tgcccgagct tcagccacta
2521 ctccgtgtgc acaagcagct gccccgacac atgctccgac ctgacggcct cgcggaactg
2581 cgccacgccg tgcacagagg gctgcgagtg caaccagggc ttcgtcctca gcaccagcca
2641 gtgcgtccct ctgcacaagt gcggctgcga cttcgacggc cactactaca ccatggggga
2701 gttcttctgg gccacggcca actgcactgt gcaatgcctg tgcgaggagg gcggggacgt
2761 ctactgcttc aacaagacct gcggcagcgg ggaggtgtgc gccgtggagg acggctacca
2821 gggctgcttc cccaagcggg agaccgtgtg cctgctcagc cagaaccagg tgctgcacac
2881 ctttgacggc gcctcctacg ccttcccctc cgagttctcc tacaccctcc tgaagacctg
2941 ccctgagcgc ccagagtact tggaaatcga catcaacaag aagaagcccg atgcaggacc
3001 tgcttggctg cggggacttc ggatcctggt ggccgaccag gaggtcaaga taggaggcat
3061 cggggcttcg gaagtcaagt tgaatggtca ggaagtggaa ttgccttttt tccatccttc
3121 ggggaagctg gaaatttatc gaaacaaaaa cagtacgaca gtggagtcca agggcgtggt
3181 gactgtccag tactcagaca taggtctatt gtacatccgg ctgtccacca catacttcaa
3241 ttgcacaggg ggcttgtgcg gcttctacaa tgccaacgcc agtgacgagt tctgtctccc
3301 caacggcaag tgcacggaca acctggcagt gttcctggaa agctggacaa ctttcgagga
3361 gatctgcaat ggagagtgtg gggacctgct gaaggcctgc aacaatgact cggagctgct
3421 caagttttat cgaagccgct ccaggtgcgg catcatcaac gacccctcca acagctcctt
3481 cctggagtgc catggggtgg tgaacgccac tgcctactac cgcacctgcc ttttccgcct
3541 gtgccagagt gggggcaatg agtcagagct ctgtgactct gtggcccggt atgcaagcgc
3601 ctgcaagaat gcggacgtgg aggtggggcc ctggcggacc tatgacttct gcccactgga
3661 gtgcccagag aacagccact ttgaggagtg catcacatgt acagagacct gtgagaccct
3721 taccctgggc cccatctgcg tggatagctg ctctgaggga tgtcagtgtg atgagggcta
3781 tgctctactg ggcagccagt gtgtcacgcg gagtgagtgt ggctgcaact ttgaggggca
3841 ccaacttgcc accaatgaga ccttctgggt ggacctggac tgccagatct tctgctattg
3901 cagtggcaca gacaacaggt aagtatcaag gttacaagac aggtttaagg agaccaataa
3961 gctattattc gtataggata ctttatacga agttattaag ttatggagac cggtgtggca
4021 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
4081 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
4141 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
4201 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
4261 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
4321 cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
4381 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
4441 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
4501 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
4561 ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga tttataaggg
4621 attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
4681 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
4741 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
4801 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
4861 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
4921 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
4981 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
5041 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
5101 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
5161 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
5221 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
5281 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
5341 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
5401 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
5461 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
5521 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
5581 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
5641 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
5701 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
5761 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
5821 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
5881 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
5941 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
6001 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
6061 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
6121 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
6181 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
6241 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
6301 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
6361 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
6421 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
6481 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
6541 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
6601 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
6661 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
6721 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
pAAVLINK-EF1a-5'-TECTA载体图谱质粒图谱pdf版和pAAVLINK-EF1a-5'-TECTA载体序列质粒序列等相关资料下载
pAAVLINK-EF1a-5'-TECTA质粒载体应用举例
- 上一篇：pAAVLINK-3'-ABCA7-FLAG
- 下一篇：没有了
