| 质粒名称: | pGEX-6p-1-hsG3BP2b |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000916 |
| 产品用途: | Express proteins in vitro |
| 存储实验室: | Peiguo YANG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pGEX-6p-1 |
| 表达系统: | 细菌表达 |
| 启动子: | tac, AmpR, Lac, LacIq |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄青霉素氯霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha, RosettaDE3, BL21 |
|
插入基因信息 |
|
| 插入基因名称: | G3BP2b |
| 物种来源: | human |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network
Yao Z., Liu Y., Chen Q., Chen X., Zhu Z., Song S., Ma X., Yang P.
Cell Rep. 43, 114617 (2024)
|
买家导航
pGEX-6p-1-hsG3BP2b载体质粒基本信息
pGEX-6p-1-hsG3BP2b质粒图谱载体图谱和pGEX-6p-1-hsG3BP2b载体序列质粒序列多克隆位点信息
pGEX-6p-1-hsG3BP2b质粒载体简介
pGEX-6p-1-hsG3BP2b质粒序列载体序列
LOCUS pGEX-6p-1-hsG3BP2b 6300 bp DNA circular SYN 04-MAR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6300)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Apr 15, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6300
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 183..211
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 219..235
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 258..911
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/label=GST
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 918..941
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQGS"
CDS 942..2287
/codon_start=1
/label=hsG3BP2b NO Codon
/translation="MVMEKPSPLLVGREFVRQYYTLLNKAPEYLHRFYGRNSSYVHGGV
DASGKPQEAVYGQNDIHHKVLSLNFSECHTKIRHVDAHATLSDGVVVQVMGLLSNSGQP
ERKFMQTFVLAPEGSVPNKFYVHNDMFRYEDEVFGDSEPELDEESEDEVEEEQEERQPS
PEPVQENANSGYYEAHPVTNGIEEPLEESSHEPEPEPESETKTEELKPQVEEKNLEELE
EKSTTPPPAEPVSLPQEPPKPRVEAKPEVQSQPPRVREQRPRERPGFPPRGPRPGRGDM
EQNDSDNRRIIRYPDSHQLFVGNLPHDIDENELKEFFMSFGNVVELRINTKGVGGKLPN
FGFVVFDDSEPVQRILIAKPIMFRGEVRLNVEEKKTRAARERETRGGGDDRRDIRRNDR
GPGGPRGIVGGGMMRDRDGRGPPPRGGMAQKLGSGRGTGQMEGRFTGQR"
promoter 2603..2707
/gene="bla"
/label=AmpR promoter
CDS 2708..3568
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3739..4327
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 4571..4648
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 4649..5731
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
protein_bind 5744..5765
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5780..5810
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5818..5834
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5842..5858
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 5854..6300
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGI"
primer_bind complement(5874..5890)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
901 atcctccaaa atcggatgaa aacctgtatt ttcagggatc catggtgatg gagaagcctt
961 caccattact ggttggtcgt gagttcgtac gccagtatta tacgttactt aacaaagcac
1021 ccgagtattt acaccgcttt tacggacgca attcgtctta tgttcacggc ggcgtggatg
1081 ctagcgggaa gcctcaagag gcggtgtatg gccagaatga tattcatcat aaagttcttt
1141 ccttgaactt tagcgaatgt cacaccaaga ttcgtcacgt agacgctcat gctacattaa
1201 gtgatggggt tgtagtccaa gttatggggt tgctgtccaa cagtggtcaa cctgagcgca
1261 aattcatgca aacctttgtg cttgcacctg aaggtagcgt acctaataag ttctatgttc
1321 ataacgatat gttccgctac gaggatgaag tgttcggtga ctcagagcct gaattagacg
1381 aagagtctga agatgaagtg gaggaggaac aagaagagcg ccaaccctcc cccgagccgg
1441 ttcaggagaa cgccaatagc ggatactacg aagcccatcc cgtaacgaac ggaatcgagg
1501 aacccctgga agagagcagt catgagcctg agccagagcc ggaatcggaa acgaaaactg
1561 aagagttaaa accccaggtc gaagaaaaaa acttagaaga gctggaggaa aagtcaacga
1621 ccccgccccc ggctgagcca gtgagtttgc cgcaagagcc tcctaaacca cgtgttgaag
1681 caaagccaga agtccagtcg cagcccccgc gtgtacgcga gcaacgccct cgtgaacgtc
1741 caggttttcc cccgcgtggt ccccgtcctg gacgtggaga tatggagcaa aatgatagcg
1801 acaaccgtcg catcatccgc taccccgaca gccaccaatt gttcgttggg aatcttccac
1861 acgatattga tgagaacgaa cttaaggagt tttttatgag cttcggtaat gtcgtggaac
1921 ttcgtatcaa cacgaagggc gtgggaggga aattgcccaa ctttggattt gttgtcttcg
1981 acgatagcga gccggtccag cgtatcttaa tcgcgaagcc aatcatgttt cgcggggagg
2041 tgcgtttaaa tgtagaggaa aaaaagaccc gcgcggctcg cgagcgtgag acccgtggag
2101 gaggtgatga tcgccgtgac attcgtcgta acgaccgcgg gcccggagga cctcgcggta
2161 ttgtaggggg agggatgatg cgtgaccgcg atggtcgcgg tcctccacca cgcggaggga
2221 tggctcaaaa actgggatct ggccgcggca ccggtcaaat ggaaggacgt ttcacgggtc
2281 aacgccgtta agaattcatc gtgactgact gacgatctgc ctcgcgcgtt tcggtgatga
2341 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
2401 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
2461 agccatgacc cagtcacgta gcgatagcgg agtgtataat tcttgaagac gaaagggcct
2521 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
2581 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
2641 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
2701 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
2761 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
2821 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
2881 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
2941 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
3001 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
3061 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
3121 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
3181 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
3241 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
3301 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
3361 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
3421 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
3481 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
3541 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
3601 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
3661 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
3721 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
3781 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
3841 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
3901 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
3961 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
4021 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
4081 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
4141 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
4201 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
4261 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
4321 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
4381 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
4441 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
4501 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
4561 cataaattcc gacaccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc
4621 ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
4681 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
4741 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
4801 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
4861 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
4921 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
4981 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
5041 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
5101 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
5161 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
5221 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
5281 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
5341 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
5401 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
5461 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca
5521 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
5581 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
5641 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
5701 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagctca
5761 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
5821 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgga ttcactggcc
5881 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
5941 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
6001 caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg
6061 gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca
6121 aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta tcccattacg
6181 gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat
6241 gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttggaatt
//
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