pYK163

LOCUS       pYK163               8069 bp DNA     circular SYN 11-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     complement(881..947)
                     /label=oYK484_NS3a_R
     primer_bind     881..900
                     /label=oYK06-ANR-mEGFP-F
     primer_bind     881..900
                     /label=oYK36-AG-Link_s-F
     primer_bind     881..900
                     /label=oYK30-NS3a_Head-F
     misc_feature    905..964
                     /label=NLS2
     primer_bind     928..986
                     /label=oYK484_NS3a_F
     primer_bind     932..985
                     /label=oYK30-NS3a_Head-F
     primer_bind     complement(943..987)
                     /label=oYK163_NLS_R
     primer_bind     complement(945..967)
                     /label=oYK54-NLS-R
     primer_bind     complement(945..967)
                     /label=oYK57-NLS-R
     CDS             968..1555
                     /codon_start=1
                     /label=NS3a H1
                     /translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
                     EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
                     SRSLTPCTCGSSDLYLVTRHADVIPVRGRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
                     AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
     primer_bind     968..1003
                     /label=oSCL1082
     primer_bind     968..988
                     /label=oyk03-03-F
     primer_bind     968..988
                     /label=oYK04-ns3a-F
     primer_bind     968..986
                     /label=oYK25-NS3a-F
     primer_bind     985..1043
                     /label=oSCL1083
     primer_bind     1024..1082
                     /label=oSCL1084
     primer_bind     1061..1119
                     /label=oSCL1085
     primer_bind     1098..1156
                     /label=oSCL1086
     primer_bind     complement(1126..1145)
                     /label=oSCL1106
     primer_bind     1127..1165
                     /label=oSCL1104
     primer_bind     complement(1362..1420)
                     /label=oSCL1105
     primer_bind     complement(1402..1473)
                     /label=oSCL1087
     primer_bind     1404..1425
                     /label=oSCL1107
     primer_bind     1455..1473
                     /label=oSCL1088
     primer_bind     complement(1537..1561)
                     /label=oSCL1089
     primer_bind     complement(1540..1572)
                     /label=oYK30-NS3a_head-R
     primer_bind     complement(1543..1584)
                     /label=oYK66-NS3a-R
     misc_feature    1562..1572
                     /label=linker
     CDS             1565..1639
                     /codon_start=1
                     /label=5xGS Linker
                     /translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
     primer_bind     1565..1585
                     /label=oYK66-AG-lplA-F
     primer_bind     1565..1583
                     /label=Primer 1
     primer_bind     complement(1614..1642)
                     /label=olyl212
     primer_bind     complement(1614..1639)
                     /label=olyl234
     primer_bind     complement(1623..1659)
                     /label=oYK45-46 ANR1-R
     primer_bind     1639..1658
                     /label=oYK36-AG-Link_s-F
     primer_bind     1640..1659
                     /label=oYK41-44 AG-LplA-F
     primer_bind     1640..1659
                     /label=oYK45-46 AG-lplA-F
     misc_feature    1640..1642
                     /label=Long_Linker
     misc_feature    1643..2317
                     /label=AG
     primer_bind     2091..2101
                     /label=oSCL1104
     primer_bind     2298..2334
                     /label=oYK37-Long_Link-F
     primer_bind     complement(2302..2317)
                     /label=oYK37-AG-R
     misc_feature    2318..2548
                     /label=Long_Linker
     primer_bind     complement(2526..2565)
                     /label=oYK37-Long_Link-R
                     /note="AGGCGTAATGTGGAgcCCATCATGGATCCTCCTTGGGTCGATC"
     misc_feature    2546..3567
                     /label=LPLA
                     /note="LplA"
     primer_bind     2546..2564
                     /label=oYK36-37-LplA-F
     misc_feature    2549
                     /label=AG
     primer_bind     2822..2844
                     /label=oYK08_lpl91-F
     primer_bind     2884..2900
                     /label=oYK09_lpl112-F
     primer_bind     2930..2945
                     /label=oYK10_lpl127-F
     primer_bind     2975..2993
                     /label=oYK11_lpl142-F
     primer_bind     3011..3029
                     /label=oYK12_lpl154-F
     primer_bind     3074..3096
                     /label=oYK13_lpl175-F
     primer_bind     3122..3140
                     /label=oYK14_lpl191-F
     primer_bind     3185..3200
                     /label=oYK15-lpl212-F
     primer_bind     3211..3230
                     /label=oYK16_lpl221-F
     primer_bind     3269..3290
                     /label=oYK17_lpl240-F
     primer_bind     3293..3311
                     /label=oYK18_lpl248-F
     primer_bind     3329..3343
                     /label=oYK19_lpl260-F
                     /note="Maybe mismatch"
     primer_bind     3365..3383
                     /label=oYK20_lpl272-F
     primer_bind     3396..3412
                     /label=oYK21_lpl283-F
     primer_bind     3443..3460
                     /label=oYK22_lpl298-F
                     /note="DNA may less than 100 bp"
     primer_bind     complement(3547..3562)
                     /label=oYK434_LplA_R
     primer_bind     complement(3552..3572)
                     /label=oYK01-1-R
     primer_bind     complement(3562..3586)
                     /label=oYK08-22-LplApart-R
     primer_bind     complement(3565..3586)
                     /label=oYK36-37-LplA-R
     primer_bind     complement(3567..3589)
                     /label=oYK57-MCP-mCherry-R
     primer_bind     complement(3591..3610)
                     /label=oSCL1089
     terminator      3663..3890
                     /label=bGH PA
     misc_feature    3936..4364
                     /label=f1 origin
     misc_feature    4369..4712
                     /label=SV40 early promoter and origin
     misc_feature    4558..4635
                     /label=SV40 ORI
     CDS             4777..5568
                     /label=Kan/neoR
     misc_feature    5742..5872
                     /label=SV40 early polyadenylation signal
     primer_bind     complement(5908..5928)
                     /label=M13-rev
     misc_binding    complement(5934..5956)
                     /label=LacO
     promoter        complement(5961..5990)
                     /label=lac
     misc_feature    complement(6255..6928)
                     /label=pUC origin
     CDS             complement(7076..7735)
                     /label=AmpR
     primer_bind     7392..7403
                     /label=oyk03-03-F
     primer_bind     7392..7403
                     /label=oYK04-ns3a-F
     promoter        complement(7975..8003)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 caccatgccc aagaagaagc gcaaggtgga agctagcgct tccccgaaga aaaagcggaa
      961 agtcatgaag aagaagggca gcgtggtgat cgtgggcaga atcaacctga gcggcgacac
     1021 cgcctacagc cagcagacca gaggcctgga gggctgccag gagaccagcc agaccggcag
     1081 agacaagaac caggtggagg gcgaggtgca ggtggtgagc accgccaccc agagcttcct
     1141 ggccaccagc atcaacggcg tgctgtggac cgtgtaccac ggcgccggca ccagaaccat
     1201 cgccagcccc aagggccccg tgacccagat gtacacgaac gtggacaagg acttagtggg
     1261 ctggcaggcc ccccaaggca gcagaagcct gaccccctgc acctgcggca gcagcgacct
     1321 gtacctggtg accagacacg ccgacgtgat ccccgtgaga ggaagaggcg acagcagagg
     1381 cagcctgctg agccccagac ccatcagcta tctgaaaggc tcaagcggcg gccccctgtt
     1441 gtgtcccgcc ggccatgccg tgggcatctt cagagccgcc gtgagcacca gaggcgtggc
     1501 caaggccgtg gacttcatcc ccgtggagag cctggagacc accatgagaa gccccgctag
     1561 cagcggaagt ggaactgggt ctgggactgg gtccggcacc gggactactt caggcaccgg
     1621 cactgggggc tccactggca tggtgagcgt gatcaagccc gagatgaaga tcaagctgtg
     1681 catgaggggc accgtgaacg gccacaactt cgtgatcgag ggcgagggca agggcaaccc
     1741 ctacgagggc acccagatcc tggacctgaa cgtgaccgag ggcgcccccc tgcccttcgc
     1801 ctacgacatc ctgaccaccg tgttccagta cggcaacagg gccttcacca agtaccccgc
     1861 cgacatccag gactacttca agcagacctt ccccgagggc taccactggg agaggagcat
     1921 gacctacgag gaccagggca tctgcaccgc caccagcaac atcagcatga ggggcgactg
     1981 cttcttctac gacatcaggt tcgacggcgt gaacttcccc cccaacggcc ccgtgatgca
     2041 gaagaagacc ctgaagtggg agcccagcac cgagaagatg tacgtgaggg acggcgtgct
     2101 gaagggcgac gtgaacatgg ccctgctgct ggagggcggc ggccactaca ggtgcgactt
     2161 caagaccacc tacaaggcca agaaggacgt gaggctgccc gactaccact tcgtggacca
     2221 caggatcgag atcctgaagc acgacaagga ctacaacaag gtgaagctgt acgagaacgc
     2281 cgtggccagg tacagcatgc tgcccagcca ggccaagacc ggttctggga gtggcggtag
     2341 tggtgctgga ggcagcgcag gctctggagc aggcggcagt gccggaagtg gcgctggagg
     2401 gtctgccgga tctggagccg gtggttctgc cggctccggc gctggtggga gcgcttccgg
     2461 aagtagtccc ggtaattccg ctgacggcgg cggaggatcg ggtggtagtg gtggttcagg
     2521 aggaggatcg acccaaggag gatccatggg ctccacatta cgcctgctca tctctgactc
     2581 ttacgacccg tggtttaacc tggcggtgga agagtgtatt tttcgccaaa tgcccgccac
     2641 gcagcgcgtt ctgtttctct ggcgcaatgc cgacacggta gtaattggtc gcgcgcagaa
     2701 cccgtggaaa gagtgtaata cccggcggat ggaagaagat aacgtccgcc tggcgcggcg
     2761 cagtagcggt ggcggcgcgg tgttccacga tctcggcaat acctgcttta cctttatggc
     2821 tggcaagccg gagtacgata aaactatctc cacgtcgatt gtgctcaatg cgctgaacgc
     2881 gctcggcgtc agcgccgaag cgtccggacg taacgatctg gtggtgaaaa ccgtcgaagg
     2941 cgaccgcaaa gtctcaggct cggcctatcg cgaaaccaaa gatcgcggct tccaccacgg
     3001 caccttgcta ctcaatgccg acctcagccg cctggcaaac tatctcaatc cggataaaaa
     3061 gaaactggcg gcgaaaggca ttacgtcggt acgttcccgc gtgaccaacc tcaccgagct
     3121 gttgccgggg atcacccatg agcaggtttg cgaggccata accgaggcct ttttcgccca
     3181 ttatggcgag cgcgtggaag cggaaatcat ctccccgaac aaaacgccag acttgccaaa
     3241 cttcgccgaa acctttgccc gccagagtag ctgggaatgg aacttcggtc aggctccggc
     3301 attctcgcat ctgctggatg aacgctttac ctggggcggc gtggaactgc atttcgacgt
     3361 tgaaaaaggc catatcaccc gcgcccaggt gtttaccgac agcctcaacc ccgcgccgct
     3421 ggaagccctc gccggacgac tgcaaggctg cctgtaccgc gcagatatgc tgcaacagga
     3481 gtgcgaagcg ctgttggttg acttcccgga acaggaaaaa gagctacggg agttatcggc
     3541 atggatggcg ggggctgtaa ggtaggggat ccactagtcc agtgtggtgg aattctgcag
     3601 atatccagca cagtggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc
     3661 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     3721 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     3781 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     3841 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     3901 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     3961 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4021 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4081 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4141 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4201 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4261 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4321 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     4381 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4441 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4501 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4561 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4621 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     4681 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     4741 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     4801 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     4861 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4921 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     4981 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5041 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5101 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5161 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5221 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5281 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     5341 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5401 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5461 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5521 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5581 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5641 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5701 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     5761 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5821 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     5881 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5941 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6001 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6061 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6121 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6181 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6241 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6301 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6361 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6421 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6481 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6541 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6601 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6661 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6721 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6781 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6841 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6901 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6961 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7021 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7081 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7141 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7201 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7261 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7321 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7381 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7441 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7501 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7561 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7621 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7681 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7741 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7801 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7861 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7921 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7981 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8041 catttccccg aaaagtgcca cctgacgtc
//