pYK224

LOCUS     pYK224          6765 bp DNA     circular SYN 11-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 27, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     881..927
                     /label=oYK30-NS3a_Head-F
     primer_bind     881..909
                     /label=oYK36-AG-Link_s-F
     primer_bind     881..909
                     /label=oYK255_AG_NS3a
     primer_bind     881..900
                     /label=oYK06-ANR-mEGFP-F
     CDS             910..1497
                     /codon_start=1
                     /label=NS3a H1
                     /translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
                     EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
                     SRSLTPCTCGSSDLYLVTRHADVIPVRGRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
                     AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
     primer_bind     910..945
                     /label=oSCL1082
     primer_bind     910..930
                     /label=oyk03-03-F
     primer_bind     910..930
                     /label=oYK04-ns3a-F
     primer_bind     910..928
                     /label=oYK25-NS3a-F
     primer_bind     927..985
                     /label=oSCL1083
     primer_bind     966..1024
                     /label=oSCL1084
     primer_bind     1003..1061
                     /label=oSCL1085
     primer_bind     1040..1098
                     /label=oSCL1086
     primer_bind     complement(1068..1087)
                     /label=oSCL1106
     primer_bind     1069..1107
                     /label=oSCL1104
     primer_bind     complement(1304..1362)
                     /label=oSCL1105
     primer_bind     complement(1344..1415)
                     /label=oSCL1087
     primer_bind     1346..1367
                     /label=oSCL1107
     primer_bind     1397..1415
                     /label=oSCL1088
     primer_bind     complement(1479..1507)
                     /label=oYK1077_NS3a_R
     primer_bind     complement(1479..1503)
                     /label=oSCL1089
     primer_bind     complement(1482..1514)
                     /label=oYK30-NS3a_head-R
     primer_bind     complement(1483..1504)
                     /label=oYK1075_NS3a_R
     primer_bind     complement(1483..1503)
                     /label=oYK1072_NS3a_R
     primer_bind     complement(1485..1526)
                     /label=oYK66-NS3a-R
     primer_bind     complement(1486..1503)
                     /label=oYK1076_NS3Flag_R
     misc_feature    1504..1514
                     /label=linker
     CDS             1507..1581
                     /codon_start=1
                     /label=5xGS Linker
                     /translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
     primer_bind     1507..1527
                     /label=oYK66-AG-lplA-F
     primer_bind     1507..1525
                     /label=Primer 1
     primer_bind     complement(1556..1584)
                     /label=olyl212
     primer_bind     complement(1556..1581)
                     /label=olyl234
     primer_bind     complement(1565..1601)
                     /label=oYK45-46 ANR1-R
     primer_bind     complement(1580..1601)
                     /label=oYK1075_NS3a_R
     primer_bind     1581..1603
                     /label=oYK255_AG_NS3a
     primer_bind     1581..1600
                     /label=oYK36-AG-Link_s-F
     primer_bind     1582..1602
                     /label=oYK1077_AG_F
     primer_bind     1582..1601
                     /label=oYK41-44 AG-LplA-F
     primer_bind     1582..1601
                     /label=oYK45-46 AG-lplA-F
     primer_bind     complement(1582..1601)
                     /label=oYK1076_NS3Flag_R
     misc_feature    1582..1584
                     /label=Long_Linker
     misc_feature    1585..2259
                     /label=AG
     primer_bind     2033..2043
                     /label=oSCL1104
     primer_bind     complement(2244..2259)
                     /label=oYK37-AG-R
     primer_bind     complement(2245..2282)
                     /label=oYK224_AG_R
     primer_bind     complement(2263..2285)
                     /label=oYK57-MCP-mCherry-R
     primer_bind     complement(2287..2306)
                     /label=oSCL1089
     terminator      2359..2586
                     /label=bGH PA
     misc_feature    2632..3060
                     /label=f1 origin
     misc_feature    3065..3408
                     /label=SV40 early promoter and origin
     misc_feature    3254..3331
                     /label=SV40 ORI
     CDS             3473..4264
                     /label=Kan/neoR
     misc_feature    4438..4568
                     /label=SV40 early polyadenylation signal
     primer_bind     complement(4604..4624)
                     /label=M13-rev
     misc_binding    complement(4630..4652)
                     /label=LacO
     promoter        complement(4657..4686)
                     /label=lac
     misc_feature    complement(4951..5624)
                     /label=pUC origin
     CDS             complement(5772..6431)
                     /label=AmpR
     primer_bind     6088..6099
                     /label=oyk03-03-F
     primer_bind     6088..6099
                     /label=oYK04-ns3a-F
     promoter        complement(6671..6699)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatga agaagaaggg cagcgtggtg atcgtgggca gaatcaacct gagcggcgac
      961 accgcctaca gccagcagac cagaggcctg gagggctgcc aggagaccag ccagaccggc
     1021 agagacaaga accaggtgga gggcgaggtg caggtggtga gcaccgccac ccagagcttc
     1081 ctggccacca gcatcaacgg cgtgctgtgg accgtgtacc acggcgccgg caccagaacc
     1141 atcgccagcc ccaagggccc cgtgacccag atgtacacga acgtggacaa ggacttagtg
     1201 ggctggcagg ccccccaagg cagcagaagc ctgaccccct gcacctgcgg cagcagcgac
     1261 ctgtacctgg tgaccagaca cgccgacgtg atccccgtga gaggaagagg cgacagcaga
     1321 ggcagcctgc tgagccccag acccatcagc tatctgaaag gctcaagcgg cggccccctg
     1381 ttgtgtcccg ccggccatgc cgtgggcatc ttcagagccg ccgtgagcac cagaggcgtg
     1441 gccaaggccg tggacttcat ccccgtggag agcctggaga ccaccatgag aagccccgct
     1501 agcagcggaa gtggaactgg gtctgggact gggtccggca ccgggactac ttcaggcacc
     1561 ggcactgggg gctccactgg catggtgagc gtgatcaagc ccgagatgaa gatcaagctg
     1621 tgcatgaggg gcaccgtgaa cggccacaac ttcgtgatcg agggcgaggg caagggcaac
     1681 ccctacgagg gcacccagat cctggacctg aacgtgaccg agggcgcccc cctgcccttc
     1741 gcctacgaca tcctgaccac cgtgttccag tacggcaaca gggccttcac caagtacccc
     1801 gccgacatcc aggactactt caagcagacc ttccccgagg gctaccactg ggagaggagc
     1861 atgacctacg aggaccaggg catctgcacc gccaccagca acatcagcat gaggggcgac
     1921 tgcttcttct acgacatcag gttcgacggc gtgaacttcc cccccaacgg ccccgtgatg
     1981 cagaagaaga ccctgaagtg ggagcccagc accgagaaga tgtacgtgag ggacggcgtg
     2041 ctgaagggcg acgtgaacat ggccctgctg ctggagggcg gcggccacta caggtgcgac
     2101 ttcaagacca cctacaaggc caagaaggac gtgaggctgc ccgactacca cttcgtggac
     2161 cacaggatcg agatcctgaa gcacgacaag gactacaaca aggtgaagct gtacgagaac
     2221 gccgtggcca ggtacagcat gctgcccagc caggccaagt aaggatccac tagtccagtg
     2281 tggtggaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgttt
     2341 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     2401 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     2461 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     2521 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     2581 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     2641 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     2701 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     2761 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     2821 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     2881 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     2941 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     3001 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     3061 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     3121 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     3181 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     3241 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3301 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     3361 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     3421 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     3481 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     3541 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     3601 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     3661 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     3721 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     3781 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     3841 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     3901 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     3961 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     4021 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     4081 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     4141 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     4201 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     4261 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     4321 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     4381 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     4441 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4501 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     4561 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     4621 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     4681 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     4741 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     4801 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     4861 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     4921 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4981 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5041 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5101 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5161 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     5221 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5281 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5341 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5401 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5461 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5521 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5581 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5641 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5701 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     5761 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     5821 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     5881 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     5941 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6001 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6061 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6121 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6181 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6241 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6301 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6361 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6421 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6481 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6541 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6601 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6661 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6721 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//