| 质粒名称: | piRFP670-Actin |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000067 |
| 产品用途: | Expresses iRFP670 fused to N-terminus of human beta actin |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | iRFP670, b-actin |
| 物种来源: | Synthetic, Homo sapiens |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Quantitative assessment of near-infrared fluorescent proteins
Zhang H., Papadaki S., Sun X., Wang X., Drobizhev M., Yao L., Rehbock M., Köster R.W., Wu L., Namikawa K., Piatkevich K.D.
Nat. Methods 2023 2010 20, 1605–1616 (2023)
|
piRFP670-Actin
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
piRFP670-Actin载体质粒基本信息
piRFP670-Actin质粒图谱载体图谱和piRFP670-Actin载体序列质粒序列多克隆位点信息
piRFP670-Actin质粒载体简介
piRFP670-Actin质粒序列载体序列
LOCUS piRFP670-Actin 6066 bp DNA circular SYN 21-APR-2023
DEFINITION Vector for fusing photoactivatable mCherry to the N-terminus of a
partner protein.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6066)
AUTHORS Clontech
TITLE Direct Submission
JOURNAL Exported Jun 25, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6066
/lab_host="Mammalian Cells"
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 585..633
/label=0416-iRFP670-F
regulatory 607..612
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 613..1545
/codon_start=1
/product="near-infrared fluorescent protein with an
emission peak at 670 nm, engineered from a bacterial
phytochrome (Shcherbakova and Verkhusha, 2013)"
/label=iRFP670
/note="derived from Rhodopseudomonas palustris RpBphP6"
/translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
primer_bind complement(1526..1566)
/label=0416-iRFP670-R
primer_bind 1546..1566
/label=Actin-F-seq
CDS 1600..2721
/codon_start=1
/label=Actin
/translation="DDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG
MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLL
TEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVP
IYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDF
EQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSI
MKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWI
GGSILASLSTFQQMWISKQEYDESGPSIVHRKCF"
primer_bind complement(2035..2055)
/label=Actin-R-seq
primer_bind complement(2702..2745)
/label=0416-actin-R
misc_feature 2725..2730
/label=MCS
/note="multiple cloning site of fluorescent protein
plasmids"
polyA_signal 2854..2975
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2945..2964)
/label=SV40-R-Universal
rep_origin complement(2982..3437)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3464..3568
/gene="bla"
/label=AmpR promoter
promoter 3570..3927
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3778..3913
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3962..4756
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 4988..5035
/label=HSV TK poly(A) signal
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 5364..5952
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggcgcg taaggtcgat ctcacctcct gcgatcgcga gccgatccac
661 atccccggca gcattcagcc gtgcggctgc ctgctagcct gcgacgcgca ggcggtgcgg
721 atcacgcgca ttacggaaaa tgccggcgcg ttctttggac gcgaaactcc gcgggtcggt
781 gagctactcg ccgattactt cggcgagacc gaagcccatg cgctgcgcaa cgcactggcg
841 cagtcctccg atccaaagcg accggcgctg atcttcggtt ggcgcgacgg cctgaccggc
901 cgcaccttcg acatctcact gcatcgccat gacggtacat cgatcatcga gttcgagcct
961 gcggcggccg aacaggccga caatccgctg cggctgacgc ggcagatcat cgcgcgcacc
1021 aaagaactga agtcgctcga agagatggcc gcacgggtgc cgcgctatct gcaggcgatg
1081 ctcggctatc accgcgtgat gttgtaccgc ttcgcggacg acggctccgg gatggtgatc
1141 ggcgaggcga agcgcagcga cctcgagagc tttctcggtc agcactttcc ggcgtcgctg
1201 gtcccgcagc aggcgcggct actgtacttg aagaacgcga tccgcgtggt ctcggattcg
1261 cgcggcatca gcagccggat cgtgcccgag cacgacgcct ccggcgccgc gctcgatctg
1321 tcgttcgcgc acctgcgcag catctcgccc tgccatctcg aatttctgcg gaacatgggc
1381 gtcagcgcct cgatgtcgct gtcgatcatc attgacggca cgctatgggg attgatcatc
1441 tgtcatcatt acgagccgcg tgccgtgccg atggcgcagc gcgtcgcggc cgaaatgttc
1501 gccgacttct tatcgctgca cttcaccgcc gcccaccacc aacgctccgg actcagatct
1561 ggcagcggtg gaggcagcgc atccggcgga agcggaagcg atgatgatat cgccgcgctc
1621 gtcgtcgaca acggctccgg catgtgcaag gccggcttcg cgggcgacga tgccccccgg
1681 gccgtcttcc cctccatcgt ggggcgcccc aggcaccagg gcgtgatggt gggcatgggt
1741 cagaaggatt cctatgtggg cgacgaggcc cagagcaaga gaggcatcct caccctgaag
1801 taccccatcg agcacggcat cgtcaccaac tgggacgaca tggagaaaat ctggcaccac
1861 accttctaca atgagctgcg tgtggctccc gaggagcacc ccgtgctgct gaccgaggcc
1921 cccctgaacc ccaaggccaa ccgcgagaag atgacccaga tcatgtttga gaccttcaac
1981 accccagcca tgtacgttgc tatccaggct gtgctatccc tgtacgcctc tggccgtacc
2041 actggcatcg tgatggactc cggtgacggg gtcacccaca ctgtgcccat ctacgagggg
2101 tatgccctcc cccatgccat cctgcgtctg gacctggctg gccgggacct gactgactac
2161 ctcatgaaga tcctcaccga gcgcggctac agcttcacca ccacggccga gcgggaaatc
2221 gtgcgtgaca ttaaggagaa gctgtgctac gtcgccctgg acttcgagca agagatggcc
2281 acggctgctt ccagctcctc cctggagaag agctacgagc tgcctgacgg ccaggtcatc
2341 accattggca atgagcggtt ccgctgccct gaggcactct tccagccttc cttcctgggc
2401 atggagtcct gtggcatcca cgaaactacc ttcaactcca tcatgaagtg tgacgtggac
2461 atccgcaaag acctgtacgc caacacagtg ctgtctggcg gcaccaccat gtaccctggc
2521 attgccgaca ggatgcagaa ggagatcact gccctggcac ccagcacaat gaagatcaag
2581 atcattgctc ctcctgagcg caagtactcc gtgtggatcg gcggctccat cctggcctcg
2641 ctgtccacct tccagcagat gtggatcagc aagcaggagt atgacgagtc cggcccctcc
2701 atcgtccacc gcaaatgctt ctagggatcc accggatcta gataactgat cataatcagc
2761 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
2821 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
2881 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
2941 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
3001 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
3061 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
3121 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
3181 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
3241 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
3301 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
3361 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
3421 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
3481 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
3541 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
3601 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
3661 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
3721 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
3781 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
3841 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
3901 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
3961 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
4021 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
4081 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
4141 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
4201 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
4261 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
4321 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
4381 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
4441 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
4501 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
4561 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
4621 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
4681 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
4741 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
4801 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
4861 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
4921 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
4981 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
5041 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
5101 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
5161 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
5221 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
5281 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
5341 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
5401 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
5461 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
5521 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
5581 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
5641 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
5701 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
5761 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
5821 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
5881 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
5941 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
6001 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
6061 atgcat
//
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