| 质粒名称: | pAAVLINK-EF1alpha-HA-5'-TRIP12 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001829 |
| 产品用途: | 5' AAVLINK plasmid for TRIP12 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TRIP12 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1alpha-HA-5'-TRIP12
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1alpha-HA-5'-TRIP12载体质粒基本信息
pAAVLINK-EF1alpha-HA-5'-TRIP12质粒图谱载体图谱和pAAVLINK-EF1alpha-HA-5'-TRIP12载体序列质粒序列多克隆位点信息
pAAVLINK-EF1alpha-HA-5'-TRIP12质粒载体简介
pAAVLINK-EF1alpha-HA-5'-TRIP12质粒序列载体序列
LOCUS pAAVLINK-EF1alpha-HA-5'-TRIP12 7217 bp DNA circular SYN 24-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7217)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 9, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7217
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..4357
/codon_start=1
/gene="TRIP12"
/gene_synonym="MRD49; TRIP-12; TRIPC; ULF"
/product="E3 ubiquitin-protein ligase TRIP12 isoform h"
/EC_number="2.3.2.26"
/label=TRIP12
/note="isoform h is encoded by transcript variant
13;thyroid receptor interacting protein 12; probable E3
ubiquitin-protein ligase TRIP12; TR-interacting protein 12;
E3 ubiquitin-protein ligase for Arf; HECT-type E3 ubiquitin
transferase TRIP12"
/db_xref="GI:1135449777"
/db_xref="CCDS:CCDS92966.1"
/db_xref="GeneID:9320"
/db_xref="HGNC:HGNC:12306"
/db_xref="MIM:604506"
/protein_id="NP_001335252.1"
/translation="SNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSP
PESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQV
PKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQL
KSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASK
SATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSP
SPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKL
ASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMAD
PESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGL
PPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNE
ETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEK
LQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANC
CQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKD
LLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGAS
NGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQW
RDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQ
RKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRH
KCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPD
IFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAAD
LGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTT
TQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGW
IKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQ"
misc_feature 823..828
/label=SDS
misc_feature 869..874
/label=SDS
misc_feature 1467..1472
/label=SDS
misc_feature 1494..1499
/label=SDS
misc_feature 1947..1952
/label=SDS
intron 4358..4398
/label=Splicing Donor
misc_feature 4402..4435
/label=lox2272-JT1571
repeat_region 4480..4620
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4480..4609
/label=AAV2 ITR
repeat_region 4481..4621
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4695..5150
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5432..5536
/gene="bla"
/label=AmpR promoter
CDS 5537..6397
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6568..7156
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc tggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatcttcca accggcctaa taacaatcca ggggggtcac tgcgacgttc
781 acagaggaac actgccgggg cccaaccaca agacgactca ataggaggaa ggtcacattt
841 agggcaggca aaacataagg gatatagccc tcctgagagt agaaaatcta attctaaggc
901 acccaaagtg cagtctaata ctacttctga actgtcaagg ggacaccttt ctaaaagaag
961 ctgcagttca tcatctgctg tgatagttcc acaaccagag gatccagaca gagccaatac
1021 ttcagaaaga caaaaaacgg ggcaggtgcc taagaaagac aattctcgag gagtgaagcg
1081 cagtgctagt ccagactaca acaggaccaa ttctcctagc tctgcaaaaa aaccaaaagc
1141 acttcagcat actgaatctc cctcagaaac aaataagcca catagtaagt caaagaagag
1201 acatttagac caggagcaac aactgaaatc tgcacaatca ccatcaacaa gcaaggctca
1261 taccaggaag agtggggcca ctggcggttc acggagtcag aaaagaaaaa ggacagagag
1321 ttcttgtgta aagagtggct ccgggtctga atcaactggt gcagaagaga gatctgcgaa
1381 acctaccaag ctggcttcaa aatcagccac ctcagccaaa gctgggtgta gcaccatcac
1441 tgattcttct tctgctgcct ctacttcctc ctcgtcttct gctgtagcct cggcctcctc
1501 cactgtacca ccaggtgcca gagtgaaaca aggaaaagat cagaacaagg ccaggcgttc
1561 ccgttcagcg tccagtccca gccccagaag aagtagcagg gaaaaggaac agagtaaaac
1621 tggtggctct tcaaaatttg attgggctgc tcgtttcagc cctaaagtta gccttcctaa
1681 aacaaaactg tctcttccag ggtcttctaa gtcagagaca tcaaaacctg gaccttctgg
1741 attacaggcc aaattagcaa gtttaagaaa atctacgaag aaacgcagtg agtctccacc
1801 tgctgagctc cccagtttga ggcggagcac acgccaaaag accacgggct cctgtgctag
1861 taccagtcgg cgaggctctg gcctgggcaa aagaggagca gctgaagctc gtcgacagga
1921 gaaaatggca gaccctgaaa gcaaccagga ggcagtaaat tcttcagctg ctcggacaga
1981 tgaagctccc caaggagctg cagcctctag ttctgttgca ggggctgttg gcatgaccac
2041 ctctggggag agtgaatcag atgattccga gatgggacgt ttgcaagctt tgttagaggc
2101 aaggggtctt ccccctcacc tatttggtcc tcttggtcct cggatgtcac agcttttcca
2161 tagaacaatt ggaagtggag ctagttctaa ggcccagcag ctactacaag gattgcaagc
2221 cagtgatgaa agtcaacagc ttcaggcagt tattgagatg tgtcagttac tggtcatggg
2281 aaatgaggag acactgggag ggtttcctgt caagagtgtt gttccagctt tgattacgtt
2341 acttcagatg gagcacaatt ttgatattat gaaccatgct tgtcgagcct taacatacat
2401 gatggaagca cttcctcgat cttctgctgt tgtagtagat gctattcctg tctttttaga
2461 aaagctgcaa gttattcagt gtattgatgt ggcagagcag gccttgactg ccttggagat
2521 gttgtcacgg agacatagta aagccattct acaggcgggt ggtttggcag actgcttgct
2581 gtacctagaa ttcttcagca taaatgccca aagaaatgca ttagcaattg cagctaattg
2641 ctgccagagt atcacgccag atgaatttca ttttgtggca gattcactcc cattgctaac
2701 ccaaaggcta acacatcagg ataaaaagtc agtagaaagc acttgccttt gttttgcacg
2761 cctagtggac aacttccagc atgaggagaa tttactccag caggttgctt ccaaagatct
2821 gcttacaaat gttcaacagc tgttggtagt gactccaccc attttaagtt ctgggatgtt
2881 tataatggtg gttcgcatgt tttctctgat gtgttccaac tgtccaactt tagctgttca
2941 acttatgaaa caaaacattg cagaaacgct tcactttctc ctgtgtggtg cctccaatgg
3001 aagttgtcag gaacagattg atcttgttcc acgaagccct caagagttgt atgaactgac
3061 atctctgatt tgtgaactta tgccatgttt accaaaagaa ggcatttttg cagttgatac
3121 catgttgaag aagggaaatg cacagaacac agatggtgcg atatggcagt ggcgtgatga
3181 tcggggcctc tggcatccat ataacaggat tgacagccgg atcattgagg cagcccatca
3241 ggtcggtgag gatgagataa gcttgtccac tctgggacga gtttatacta ttgattttaa
3301 ttctatgcag caaatcaatg aggacacggg aacagcacgt gccattcaga gaaaacctaa
3361 cccgttagcc aatagtaaca ctagtggata ttcagagtca aagaaggatg atgctcgagc
3421 acagcttatg aaagaggatc cggaactggc taagtctttt attaagacat tatttggtgt
3481 tctttatgaa gtgtatagtt cctcagcagg acctgcggtc agacataagt gccttagagc
3541 aattcttagg ataatttatt ttgcggatgc tgaacttctg aaggatgttc tgaaaaatca
3601 tgctgtttca agtcacattg cttccatgct gtcaagccaa gacctgaaga tagtagtggg
3661 agcacttcag atggcagaaa ttttaatgca gaagttacct gatattttta gtgtttactt
3721 cagaagagaa ggtgtaatgc atcaagtaaa acacttagca gaatcagagt ctttgttgac
3781 aagtccacca aaggcatgta cgaatggatc gggatccatg ggatccacaa cttcagtcag
3841 cagtgggaca gccacagctg ccactcatgc tgcagctgac ttgggatcac ccagcttgca
3901 gcacagcagg gatgattctt tagatctcag ccctcaaggt cgattaagtg atgttctaaa
3961 gagaaaacga ctgccaaaac gagggccaag aaggccaaag tactcacctc caagagatga
4021 tgacaaagta gacaatcaag ctaaaagccc caccactact cagtcaccta aatcttcttt
4081 cctggcaagc ttgaatccaa aaacatgggg aaggttaagt acacagtcca acagcaacaa
4141 cattgagcca gcacggactg cgggaggtag tggccttgcc agggctgcct caaaggatac
4201 catctccaat aatagagaaa aaattaaagg ttggattaag gagcaggcac ataaatttgt
4261 agaacgttat ttcagttctg agaatatgga tggaagcaac cctgcattga atgtccttca
4321 gagactttgt gctgcaaccg aacaactcaa cctccaggta agtatcaagg ttacaagaca
4381 ggtttaagga gaccaataag ctattattcg tataggatac tttatacgaa gttattaagt
4441 tatggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
4501 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
4561 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
4621 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
4681 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
4741 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
4801 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
4861 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
4921 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
4981 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
5041 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
5101 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
5161 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
5221 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
5281 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
5341 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
5401 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
5461 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
5521 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
5581 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
5641 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
5701 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
5761 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
5821 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
5881 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
5941 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
6001 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
6061 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
6121 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
6181 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
6241 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
6301 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
6361 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
6421 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
6481 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
6541 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
6601 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
6661 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
6721 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
6781 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
6841 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
6901 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
6961 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
7021 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
7081 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
7141 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
7201 gccttttgct cacatgt
//
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pAAVLINK-EF1alpha-HA-5'-TRIP12质粒载体应用举例
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