| 质粒名称: | pAAVS1-P-CAG-GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 80491 |
| 存储实验室: | Knut Woltjen |
|
载体骨架基本信息 |
|
| 载体名称: | pCR-TOPO |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素和卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 3000 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CAG-GFP |
| 物种来源: | -- |
| 插入大小: | 2447 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pAAVS1-P-CAG-GFP was a gift from Knut Woltjen (Addgene plasmid # 80491 ; http://n2t.net/addgene:80491 ; RRID:Addgene_80491) |
| 参考文献部分: | A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi. Nodvig CS, Nielsen JB, Kogle ME, Mortensen UH. PLoS One. 2015 Jul 15;10(7):e0133085. doi: 10.1371/journal.pone.0133085. eCollection 2015. PONE-D-15-11561 [pii] PubMed 26177455 |
买家导航
pAAVS1-P-CAG-GFP载体质粒基本信息
pAAVS1-P-CAG-GFP质粒图谱载体图谱和pAAVS1-P-CAG-GFP载体序列质粒序列多克隆位点信息
pAAVS1-P-CAG-GFP质粒载体简介
pAAVS1-P-CAG-GFP质粒序列载体序列
LOCUS pAAVS1-P-CAG-GFP 9608 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION donor vector for AAVS1 targeting (puromycin selection) and
constitutive GFP expression.
ACCESSION .
VERSION .
KEYWORDS pAAVS1-P-CAG-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9608)
AUTHORS Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T,
Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
TITLE Engineering the AAVS1 locus for consistent and scalable transgene
expression in human iPSCs and their differentiated derivatives.
JOURNAL Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi:
10.1016/j.ymeth.2015.12.012.
PUBMED 26707206
REFERENCE 2 (bases 1 to 9608)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9608
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 107..128
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 186..208
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 205..221
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 242..260
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
misc_feature 295..1098
/label=HA-L
/note="left homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
misc_feature 1105..1130
/label=SA
/note="splice acceptor site"
CDS 1154..1207
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 1217..1816
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1217..1236)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 1713..1733
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind complement(1848..1865)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 1854..2078
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
enhancer 2082..2461
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2464..2739
/label=chicken beta-actin promoter
intron 2740..3748
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 3672..3694
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 3756..3775
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
regulatory 3803..3812
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3809..4528
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3854..3875)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4115..4134)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4462..4483
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(4612..4631)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 4677..4732
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(4731..4750)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
misc_feature 5104..5940
/label=HA-R
/note="right homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
primer_bind complement(5980..5999)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(5981..5999)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6006..6023)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6006..6022)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6015..6037)
/label=M13/pUC Forward
/note="In lacZ gene"
CDS 6160..6462
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 6341..6360
/label=ccdB-fwd
/note="ccdB gene, forward primer"
CDS 6811..7605
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6865..6884)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7475..7494
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
CDS complement(7855..8715)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8478..8497
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8839..9427
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9328..9347
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9581..9598
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt
301 ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg
361 ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct
421 ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc
481 acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg
541 tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc
601 ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg
661 acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt
721 ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt
781 tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg
841 ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca
901 tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat
961 gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc
1021 accttatatt cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc
1081 tccaccccac agtggggcaa gcttctgacc tcttctcttc ctcccacagg gcctcgagag
1141 atctggcagc ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc
1201 cggccctagg ctcgagatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
1261 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
1321 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
1381 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
1441 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
1501 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
1561 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
1621 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
1681 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
1741 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
1801 caagcccggt gcctgatcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
1861 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
1921 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
1981 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
2041 caatagcagg catgctgggg atgcggtggg ctctatgggt cgacattgat tattgactag
2101 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
2161 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
2221 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
2281 ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
2341 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
2401 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
2461 gggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc
2521 caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg
2581 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
2641 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
2701 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gttgccttcg
2761 ccccgtgccc cgctccgcgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
2821 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
2881 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
2941 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
3001 cgcgtgcggc ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
3061 gcgctccgcg tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg
3121 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
3181 gggcgcggcg gtcgggctgt aacccccccc tgcacccccc tccccgagtt gctgagcacg
3241 gcccggcttc gggtgcgggg ctccgtgcgg ggcgtggcgc ggggctcgcc gtgccgggcg
3301 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct
3361 cgggggaggg gcgcggcggc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
3421 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
3481 gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg gcgaagcggt
3541 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
3601 cccttctcca tctccagcct cggggctgcc gcagggggac ggctgccttc gggggggacg
3661 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
3721 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
3781 tcatcatttt ggcaaagaat tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
3841 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
3901 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
3961 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
4021 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
4081 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
4141 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
4201 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
4261 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
4321 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
4381 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
4441 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
4501 tctcggcatg gacgagctgt acaagtaaga attcactcct caggtgcagg ctgcctatca
4561 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
4621 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
4681 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
4741 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
4801 aacatatgcc atatgctggc tgccatgaac aaaggtggct ataaagaggt catcagtata
4861 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
4921 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
4981 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
5041 ttctcttatg aagatccctc gacctgcagc ccaagcttgg atccctcgac agtactaagc
5101 tttactaggg acaggattgg tgacagaaaa gccccatcct taggcctcct ccttcctagt
5161 ctcctgatat tgggtctaac ccccacctcc tgttaggcag attccttatc tggtgacaca
5221 cccccatttc ctggagccat ctctctcctt gccagaacct ctaaggtttg cttacgatgg
5281 agccagagag gatcctggga gggagagctt ggcagggggt gggagggaag ggggggatgc
5341 gtgacctgcc cggttctcag tggccaccct gcgctaccct ctcccagaac ctgagctgct
5401 ctgacgcggc tgtctggtgc gtttcactga tcctggtgct gcagcttcct tacacttccc
5461 aagaggagaa gcagtttgga aaaacaaaat cagaataagt tggtcctgag ttctaacttt
5521 ggctcttcac ctttctagtc cccaatttat attgttcctc cgtgcgtcag ttttacctgt
5581 gagataaggc cagtagccag ccccgtcctg gcagggctgt ggtgaggagg ggggtgtccg
5641 tgtggaaaac tccctttgtg agaatggtgc gtcctaggtg ttcaccaggt cgtggccgcc
5701 tctactccct ttctctttct ccatccttct ttccttaaag agtccccagt gctatctggg
5761 acatattcct ccgcccagag cagggtcccg cttccctaag gccctgctct gggcttctgg
5821 gtttgagtcc ttggcaagcc caggagaggc gctcaggctt ccctgtcccc cttcctcgtc
5881 caccatctca tgcccctggc tctcctgccc cttccctaca ggggttcctg gctctgctct
5941 aagggcaagg gcgaattcgc ggccgctaaa ttcaattcgc cctatagtga gtcgtattac
6001 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
6061 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
6121 gatcgccctt cccaacagtt gcgcagccta tacgtacggc agtttaaggt ttacacctat
6181 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccg
6241 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
6301 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
6361 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
6421 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg catgagatta
6481 tcaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc
6541 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag
6601 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg
6661 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
6721 aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagctct
6781 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
6841 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
6901 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
6961 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
7021 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
7081 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
7141 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
7201 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
7261 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
7321 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
7381 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
7441 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
7501 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
7561 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaattat taacgcttac
7621 aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatcagg
7681 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
7741 aaatatgtat ccgctcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
7801 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
7861 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
7921 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
7981 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
8041 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
8101 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
8161 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
8221 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
8281 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
8341 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
8401 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
8461 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
8521 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
8581 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
8641 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
8701 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
8761 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
8821 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
8881 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
8941 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
9001 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
9061 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
9121 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
9181 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
9241 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
9301 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
9361 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
9421 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
9481 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
9541 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
9601 agcggaag
//
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pAAVS1-P-CAG-GFP质粒载体应用举例
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