| 质粒名称: | pUltra-Plus-H2B-mTagBFP2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 161790 |
| 存储实验室: | Jorge Luis Arias-Arias |
|
载体骨架基本信息 |
|
| 载体名称: | pUltra-Plus (Addgene Plasmid #161775) |
| 载体出品公司: | Jorge L. Arias-Arias |
| 空载体大小: | 7611bp |
| 完整质粒大小: | 8706bp |
| 载体类型: | 哺乳细胞表达, Lentiviral ; Bicistronic |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | H2B-mTagBFP2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1095bp |
| 基因突变: | TI174A in the mTagBFP2 protein according to PLoS ONE (2011) 6: e28674. doi:10.1371/journal.pone.0028674 |
| 启动子: | UbC |
| 标签/融合蛋白: | mTagBFP2 (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | XbaI (not destroyed) |
| 3’克隆位点: | HI (not destroyed) |
| 5’测序引物 | hUBCpro-F |
|
文献引用方法 |
|
| 材料和方法部分: |
pUltra-Plus-H2B-mTagBFP2 was a gift from Jorge Luis Arias-Arias (Addgene plasmid # 161790 ; http://n2t.net/addgene:161790 ; RRID:Addgene_161790)
|
买家导航
pUltra-Plus-H2B-mTagBFP2载体质粒基本信息
pUltra-Plus-H2B-mTagBFP2质粒图谱载体图谱和pUltra-Plus-H2B-mTagBFP2载体序列质粒序列多克隆位点信息
pUltra-Plus-H2B-mTagBFP2质粒载体简介
pUltra-Plus-H2B-mTagBFP2质粒序列载体序列
LOCUS pUltra-Plus-H2B-mTagBFP2 8706 bp DNA circular SYN 25-AUG-2024
DEFINITION 3rd generation lentiviral bicistronic vector for mammalian
expression of H2B-mTagBFP2 and one gene of interest.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8706)
TITLE pUltra series
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8706)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
FEATURES Location/Qualifiers
source 1..8706
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..1212
/label=UbC promoter
/note="human ubiquitin C promoter"
primer_bind 1049..1068
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
CDS 1262..1318
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 1325..1702
/codon_start=1
/gene="HIST1H2BJ"
/product="human histone H2B"
/label=H2B
/translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
GELAKHAVSEGTKAITKYTSAK"
CDS 2435..2488
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
misc_feature 2532..3120
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2585..2605)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3003..3014)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 3211..3444
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 3516..3637
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3553..3572)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3607..3626
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 3677..3812
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(3832..3851)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(3833..3851)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3861..3878)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(3861..3877)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3870..3892)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 4019..4474
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4106..4125)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4316..4337
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 4500..4604
/gene="bla"
/label=AmpR promoter
CDS 4605..5465
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4823..4842)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5636..6224
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6125..6144
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6378..6395
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 6512..6533
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6548..6578
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6586..6602
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6591..6613
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 6610..6626
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6610..6626
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 6645..6665
/label=T3
/note="T3 promoter, forward primer"
promoter 6647..6665
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 6693..6919
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 6920..7100
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 7147..7272
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 7765..7998
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 8183..8227
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 8525..8642
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
ORIGIN
1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
1201 tttttgttag acgaagcttg ggctgcaggt ccgatccacc ggtgatatca tgggatctgg
1261 agcaacaaac ttctcactac tcaaacaagc aggtgacgtg gaggagaatc ccggaccttc
1321 tagaatgcca gagccagcga agtctgctcc cgccccgaaa aagggctcca agaaggcggt
1381 gactaaggcg cagaagaaag gcggcaagaa gcgcaagcgc agccgcaagg agagctattc
1441 catctatgtg tacaaggttc tgaagcaggt ccaccctgac accggcattt cgtccaaggc
1501 catgggcatc atgaactcgt ttgtgaacga cattttcgag cgcatcgcag gtgaggcttc
1561 ccgcctggcg cattacaaca agcgctcgac catcacctcc agggagatcc agacggccgt
1621 gcgcctgctg ctgcctgggg agttggccaa gcacgccgtg tccgagggta ctaaggccat
1681 caccaagtac accagcgcta aggaccctcc agtcgccacc atgagcgagt tgatcaagga
1741 aaacatgcat atgaagctct acatggaagg caccgtggac aaccaccatt tcaagtgtac
1801 ctccgaggga gagggaaagc cttacgaggg aactcagacc atgcgcatca aggtcgtgga
1861 aggcggtcca ctgcccttcg ctttcgacat tctcgccacc tcattcctct acggctccaa
1921 gactttcatc aatcataccc agggtattcc cgatttcttc aagcaatcgt tcccggaagg
1981 gtttacctgg gaacgcgtga ccacttacga ggacggaggg gtcctgaccg caactcagga
2041 cactagcctg caggacggtt gcctgatcta taacgtgaag attcggggag tgaacttcac
2101 ctccaacgga cctgtcatgc aaaagaaaac cctgggctgg gaagccttta ccgagactct
2161 gtatccggcc gacggcggac ttgaggggag aaatgatatg gcgctgaaac tggtcggcgg
2221 ctcccacctg atcgccaacg ccaagacgac ctaccggtcg aagaagcccg ccaagaacct
2281 gaagatgccg ggagtgtact acgtggacta caggctggag cggatcaagg aagccaacaa
2341 cgaaacatac gtggaacagc acgaagtggc cgtggcgcgc tactgcgatc tccctagcaa
2401 actggggcac aaactcaatg gatccggctc cggcgagggc aggggaagtc tactaacatg
2461 cggggacgtg gaggaaaatc ccggcccagc tagcgtgtga tcagaattcg ttccggagtc
2521 gtcgactcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
2581 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
2641 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
2701 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
2761 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
2821 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
2881 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
2941 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
3001 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
3061 ttccgcatct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
3121 ctggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
3181 ttagccactt tttaaaagaa aaggggggac tggaagggct acgtaactcc caacgaagac
3241 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
3301 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
3361 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
3421 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
3481 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
3541 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
3601 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
3661 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
3721 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
3781 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
3841 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
3901 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
3961 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
4021 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
4081 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
4141 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
4201 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
4261 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
4321 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
4381 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
4441 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
4501 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
4561 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
4621 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
4681 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
4741 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
4801 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
4861 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
4921 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
4981 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
5041 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
5101 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
5161 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
5221 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
5281 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
5341 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
5401 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
5461 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
5521 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
5581 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
5641 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
5701 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
5761 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
5821 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
5881 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
5941 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
6001 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
6061 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
6121 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tcacttgagc
6181 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
6241 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
6301 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
6361 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
6421 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
6481 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
6541 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
6601 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
6661 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
6721 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
6781 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
6841 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
6901 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
6961 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
7021 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
7081 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
7141 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
7201 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
7261 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
7321 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
7381 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
7441 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
7501 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
7561 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
7621 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
7681 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
7741 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
7801 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
7861 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
7921 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
7981 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
8041 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
8101 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
8161 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
8221 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
8281 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
8341 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
8401 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
8461 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
8521 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
8581 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
8641 ttcgggttta ttacagggac agcagagatc cagtttggtt aattaacccg tgtcggctcc
8701 agatct
//
