| 质粒名称: | pAAVLINKv1-pCALM1-Cre |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001725 |
| 产品用途: | 3' AAVLINKv1 backbone vector |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | No |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre载体质粒基本信息
pAAVLINKv1-pCALM1-Cre质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre质粒载体简介
pAAVLINKv1-pCALM1-Cre质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre 4520 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4520)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4520
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
terminator 1309..1740
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 1453..1458
/label=SDS
repeat_region 1783..1923
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 1783..1912
/label=AAV2 ITR
rep_origin 1998..2453
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2735..2839
/gene="bla"
/label=AmpR promoter
CDS 2840..3700
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3871..4459
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattagct cgagataatc
1321 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
1381 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
1441 ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg gaactcatcg
1501 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
1561 tgtttatttg tgaaatttgt gatgctattg ctttatttgt aaccatctag ctttatttgt
1621 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
1681 aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa
1741 agatctggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
1801 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
1861 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
1921 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
1981 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
2041 tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt
2101 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
2161 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
2221 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
2281 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg
2341 ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct
2401 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
2461 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
2521 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
2581 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
2641 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
2701 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
2761 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
2821 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
2881 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
2941 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
3001 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
3061 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
3121 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
3181 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
3241 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
3301 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
3361 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
3421 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
3481 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
3541 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
3601 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
3661 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
3721 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
3781 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
3841 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
3901 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
3961 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
4021 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
4081 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
4141 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
4201 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
4261 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
4321 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
4381 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
4441 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
4501 ctggcctttt gctcacatgt
//
