pMT525

LOCUS       pMT525               17897 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  L1RP retrotransposition reporter.
ACCESSION   .
VERSION     .
KEYWORDS    pMT525
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17897)
  AUTHORS   Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov
            M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, 
            Smogorzewska A, Holland AJ, Burns KH
  TITLE     Cell fitness screens reveal a conflict between LINE-1 
            retrotransposition and DNA replication.
  JOURNAL   Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 
            10.1038/s41594-020-0372-1. Epub 2020 Feb 10.
  PUBMED    32042151
REFERENCE   2  (bases 1 to 17897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17897
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     polyA_signal    6665..6713
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(6831..6850)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6922..6943)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(7978..8496)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     8171..8190
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8430..8451
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8493..8502
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(8569..8772)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        8773..9076
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    9205..9339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9229..9248)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9283..9302
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(9482..9504)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10068..11857
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(13940..13958)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        14026..14130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             14131..14991
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14349..14368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15162..15750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15651..15670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        15945..16444
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(15980..16003)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     16377..16396
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             16484..17083
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(16484..16503)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     16980..17000
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acgtaactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttgacagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcaagcttgc tagcggccgc tctagccctg
      661 gaatgtgtat taagactgta aggtgggggg gaggagccaa gatggccgaa taggaacagc
      721 tccggtctac agctcccagc gtgagcgacg cagaagacgg tgatttctgc atttccatct
      781 gaggtaccgg gttcatctca ctagggagtg ccagacagtg ggcgcaggcc agtgtgtgtg
      841 cgcaccgtgc gcgagccgaa gcagggcgag gcattgcctc acctgggaag cgcaaggggt
      901 cagggagttc cctttccgag tcaaagaaag gggtgacgga cgcacctgga aaatcgggtc
      961 actcccaccc gaatattgcg cttttcagac cggcttaaga aacggcgcac cacgagacta
     1021 tatcccgcac ctggctcgga gggtcctacg cccacggaat ctcgctgatt gctagcacag
     1081 cagtctgaga tcaaactgca aggcggcaac gaggctgggg gaggggcgcc cgccattgcc
     1141 caggcttgct taggtaaaca aagcagcagg gaagctcgaa ctgggtggag cccaccacag
     1201 ctcaaggagg cctgcctgcc tctgtaggct ccacctctgg gggcagggca cagacaaaca
     1261 aaaagacagc agtaacctct gcagacttaa gtgtccctgt ctgacagctt tgaagagagc
     1321 agtggttctc ccagcacgca gctggagatc tgagaacggg cagactgcct cctcaagtgg
     1381 gtccctgacc cctgaccccc gagcagccta actgggaggc accccccagc aggggcacac
     1441 tgacacctca cacggcaggg tattccaaca gacctgcagc tgagggtcct gtctgttaga
     1501 aggaaaacta acaaccagaa aggacatcta caccgaaaac ccatctgtac atcaccatca
     1561 tcaaagacca aaagtagata aaaccacaaa gatggggaaa aaacagaaca gaaaaactgg
     1621 aaactctaaa acgcagagcg cctctcctcc tccaaaggaa cgcagttcct caccagcaac
     1681 agaacaaagc tggatggaga atgattttga tgagctgaga gaagaaggct tcagacgatc
     1741 aaattactct gagctacggg aggacattca aaccaaaggc aaagaagttg aaaactttga
     1801 aaaaaattta gaagaatgta taactagaat aaccaataca gagaagtgct taaaggagct
     1861 gatggagctg aaaaccaagg ctcgagaact acgtgaagaa tgcagaagcc tcaggagccg
     1921 atgcgatcaa ctggaagaaa gggtatcagc aatggaagat gaaatgaatg aaatgaagcg
     1981 agaagggaag tttagagaaa aaagaataaa aagaaatgag caaagcctcc aagaaatatg
     2041 ggactatgtg aaaagaccaa atctacgtct gattggtgta cctgaaagtg atgtggagaa
     2101 tggaaccaag ttggaaaaca ctctgcagga tattatccag gagaacttcc ccaatctagc
     2161 aaggcaggcc aacgttcaga ttcaggaaat acagagaacg ccacaaagat actcctcgag
     2221 aagagcaact ccaagacaca taattgtcag attcaccaaa gttgaaatga aggaaaaaat
     2281 gttaagggca gccagagaga aaggtcgggt taccctcaaa ggaaagccca tcagactaac
     2341 agcggatctc tcggcagaaa ccctacaagc cagaagagag tgggggccaa tattcaacat
     2401 tcttaaagaa aagaattttc aacccagaat ttcatatcca gccaaactaa gcttcataag
     2461 tgaaggagaa ataaaatact ttatagacaa gcaaatgttg agagattttg tcaccaccag
     2521 gcctgcccta aaagagctcc tgaaggaagc gctaaacatg gaaaggaaca accggtacca
     2581 gccgctgcaa aatcatgcca aaatgtaaag accatcaaga ctaggaagaa actgcaccaa
     2641 ctaatgagca aaatcaccag ctaacatcat aatgacagga tcaacttcac acataacaat
     2701 attaacttta aatataaatg gactaaattc tgcaattaaa agacacagac tggcaagttg
     2761 gataaagagt caagacccat cagtgtgctg tattcaggaa acccatctca cgtgcagaga
     2821 cacacatagg ctcaaaataa aaggatggag gaagatctac caagccaatg gaaaacaaaa
     2881 aaaggcaggg gttgcaatcc tagtctctga taaaacagac tttaaaccaa caaagatcaa
     2941 aagagacaaa gaaggccatt acataatggt aaagggatca attcaacaag aggagctaac
     3001 tatcctaaat atttatgcac ccaatacagg agcacccaga ttcataaagc aagtcctcag
     3061 tgacctacaa agagacttag actcccacac attaataatg ggagacttta acaccccact
     3121 gtcaacatta gacagatcaa cgagacagaa agtcaacaag gatacccagg aattgaactc
     3181 agctctgcac caagcagacc taatagacat ctacagaact ctccacccca aatcaacaga
     3241 atatacgttt ttttcagcac cacaccacac ctattccaaa attgaccaca tagttggaag
     3301 caaagctctc ctcagcaaat gtaaaagaac agaaattata acaaactatc tctcagacca
     3361 cagtgcaatc aaactagagc tcaggattaa gaatctcact caaagccgct caactacatg
     3421 gaaactgaac aacctgctcc tgaatgacta ctgggtacat aacgaaatga aggcagaaat
     3481 aaagatgttc tttgaaacca acgagaacaa agacaccaca taccagaatc tctgggacgc
     3541 attcaaagca gtatgtagag ggaaatttat agcactaaat gcctacaaga gaaagcagga
     3601 aagatccaaa attgacaccc taacatcaca attaaaagaa ctagaaaagc aagagcaaac
     3661 acattcaaaa gctagcagaa ggcaagaaat aactaaaatc agagcagaac tgaaggaaat
     3721 agagacacaa aaaacccttc aaaaaatcaa tgaatccagg agctggtttt ttgaaaggat
     3781 caacaaaatt gatagaccgc tagcaagact aataaagaaa aaaagagaga agaatcaaat
     3841 agacacaata aaaaatgata aaggggatat caccaccgat cccacagaaa tacaaactac
     3901 catcagagaa tactacaaac acctctacgc aaataaacta gaaaatctag aagaaatgga
     3961 tacattcctc gacacataca ctctcccaag actaaaccag gaagaagttg aatctctgaa
     4021 tcgaccaata acaggctctg aaattgtggc aataatcaat agtttaccaa ccaaaaagag
     4081 tccaggacca gatggattca cagccgaatt ctaccagagg tacaaggagg aactggtacc
     4141 attccttctg aaactattcc aatcaataga aaaagaggga atcctcccta actcatttta
     4201 tgaggccagc atcattctga taccaaagcc gggcagagac acaaccaaaa aagagaattt
     4261 tagaccaata tccttgatga acattgatgc aaaaatcctc aataaaatac tggcaaaccg
     4321 aatccagcag cacatcaaaa agcttatcca ccatgatcaa gtgggcttca tccctgggat
     4381 gcaaggctgg ttcaatatac gcaaatcaat aaatgtaatc cagcatataa acagagccaa
     4441 agacaaaaac cacatgatta tctcaataga tgcagaaaaa gcctttgaca aaattcaaca
     4501 acccttcatg ctaaaaactc tcaataaatt aggtattgat gggacgtatt tcaaaataat
     4561 aagagctatc tatgacaaac ccacagccaa tatcatactg aatgggcaaa aactggaagc
     4621 attccctttg aaaaccggca caagacaggg atgccctctc tcaccgctcc tattcaacat
     4681 agtgttggaa gttctggcca gggcaatcag gcaggagaag gaaataaagg gtattcaatt
     4741 aggaaaagag gaagtcaaat tgtccctgtt tgcagacgac atgattgttt atctagaaaa
     4801 ccccatcgtc tcagcccaaa atctccttaa gctgataagc aacttcagca aagtctcagg
     4861 atacaaaatc aatgtacaaa aatcacaagc attcttatac accaacaaca gacaaacaga
     4921 gagccaaatc atgggtgaac tcccattcac aattgcttca aagagaataa aatacctagg
     4981 aatccaactt acaagggatg tgaaggacct cttcaaggag aactacaaac cactgctcaa
     5041 ggaaataaaa gaggagacaa acaaatggaa gaacattcca tgctcatggg taggaagaat
     5101 caatatcgtg aaaatggcca tactgcccaa ggtaatttac agattcaatg ccatccccat
     5161 caagctacca atgactttct tcacagaatt ggaaaaaact actttaaagt tcatatggaa
     5221 ccaaaaaaga gcccgcattg ccaagtcaat cctaagccaa aagaacaaag ctggaggcat
     5281 cacactacct gacttcaaac tatactacaa ggctacagta accaaaacag catggtactg
     5341 gtaccaaaac agagatatag atcaatggaa cagaacagag ccctcagaaa taatgccgca
     5401 tatctacaac tatctgatct ttgacaaacc tgagaaaaac aagcaatggg gaaaggattc
     5461 cctatttaat aaatggtgct gggaaaactg gctagccata tgtagaaagc tgaaactgga
     5521 tcccttcctt acaccttata caaaaatcaa ttcaagatgg attaaagatt taaacgttaa
     5581 acctaaaacc ataaaaaccc tagaagaaaa cctaggcatt accattcagg acataggcgt
     5641 gggcaaggac ttcatgtcca aaacaccaaa agcaatggca acaaaagaca aaattgacaa
     5701 atgggatcta attaaactaa agagcttctg cacagcaaaa gaaactacca tcagagtgaa
     5761 caggcaacct acaacatggg agaaaatttt tgcaacctac tcatctgaca aagggctaat
     5821 atccagaatc tacaatgaac tcaaacaaat ttacaagaaa aaaacaaaca accccatcaa
     5881 aaagtgggcg aaggacatga acagacactt ctcaaaagaa gacatttatg cagccaaaaa
     5941 acacatgaag aaatgctcat catcactggc catcagagaa atgcaaatca aaaccactat
     6001 gagatatcat ctcacaccag ttagaatggc aatcattaaa aagtcaggaa acaacaggtg
     6061 ctggagagga tgcggagaaa taggaacact tttacactgt tggtgggact gtaaactagt
     6121 tcaaccattg tggaagtcag tgtggcgatt cctcagggat ctagaactag aaataccatt
     6181 tgacccagcc atcccattac tgggtatata cccaaatgag tataaatcat gctgctataa
     6241 agacacatgc acacgtatgt ttattgcggc actattcaca atagcaaaga cttggaacca
     6301 acccaaatgt ccaacaatga tagactggat taagaaaatg tggcacatat acaccatgga
     6361 atactatgca gccataaaaa atgatgagtt catatccttt gtagggacat ggatgaaatt
     6421 ggaaaccatc attctcagta aactatcgca agaacaaaaa accaaacacc gcatattctc
     6481 actcataggt gggaattgaa caatgagatc acatggacac aggaagggga atatcacact
     6541 ctggggactg tggtggggtc gggggagggg ggagggatag cattgggaga tatacctaat
     6601 gctagatgac acattagtgg gtgcagcgca ccagcatggc acatgtaaaa cgacggatcg
     6661 atccgaacaa acgacccaac acccgtgcgt tttattctgt ctttttattg ccgatcccct
     6721 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     6781 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     6841 gtagccaacg ctatgtcctg atagcggtcg gccgctttac ttgtacagct cgtccatgcc
     6901 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
     6961 ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg
     7021 gccgtcgccg atgggggtgt tctgctggta gtggtcggcc aggtgagtcc aggagatgtt
     7081 tcagcactgt tgcctttagt ctcgaggcaa cttagacaac tgagtattga tctgagcaca
     7141 gcagggtgtg agctgtttga agatactggg gttgggggtg aagaaactgc agaggactaa
     7201 ctgggctgag acccagtggc aatgttttag ggcctaagga atgcctctga aaatctagat
     7261 ggacaacttt gactttgaga aaagagaggt ggaaatgagg aaaatgactt ttctttatta
     7321 gatttcggta gaaagaactt tcatctttcc cctatttttg ttattcgttt taaaacatct
     7381 atctggaggc aggacaagta tggtcattaa aaagatgcag gcagaaggca tatattggct
     7441 cagtcaaagt ggggaacttt ggtggccaaa catacattgc taaggctatt cctatatcag
     7501 ctggacacat ataaaatgct gctaatgctt cattacaaac ttatatcctt taattccaga
     7561 tgggggcaaa gtatgtccag gggtgaggaa caattgaaac atttgggctg gagtagattt
     7621 tgaaagtcag ctctgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgcgc gcacgtgtgt
     7681 ttgtgtgtgt gtgagagcgt gtgtttcttt taacgttttc agcctacagc atacagggtt
     7741 catggtggca agaagataac aagatttaaa ttatggccag tgactagtgc tgcaagaaga
     7801 acaactacct gcatttaatg ggaaagcaaa atctcaggct ttgagggaag ttaacatagg
     7861 cttgattctg ggtggaagct gggtgtgtag ttatctggag gccaggctgg agctctcagc
     7921 tcactatggg ttcatcttta ttgtctcctt tcatctcaac agctgcacgc tgccgtcctc
     7981 gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat
     8041 atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc
     8101 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac
     8161 ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta
     8221 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct
     8281 gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt
     8341 gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
     8401 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
     8461 caccccggtg aacagctcct cgcccttgct caccatggtg gcgaattcga agcttgagct
     8521 cgagatctga gtccggtagc gctagcggat ctgacggttc actaaaccag ctctgcttat
     8581 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
     8641 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
     8701 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
     8761 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
     8821 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
     8881 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
     8941 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata
     9001 cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac
     9061 cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga
     9121 ttactattag cccgggcaat gtgcacatgt accctaaaac ttaaagtata ataaagacgt
     9181 cagggttcga aatcgataag cttggatcca gacatgataa gatacattga tgagtttgga
     9241 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     9301 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     9361 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     9421 aaatgtggta tggctgatta tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa
     9481 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     9541 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     9601 ggtcgatcga ctctagagga tcgatccccg ccccggacga actaaacctg actacgacat
     9661 ctctgcccct tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc
     9721 caactgggcc ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga
     9781 ctgtagttga catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc
     9841 ctggagcaga ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt
     9901 ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta
     9961 cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc
    10021 aagagggcat tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata
    10081 tgcttcccgg gtagtagtat atactatcca gactaaccct aattcaatag catatgttac
    10141 ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat
    10201 atctcccacc ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt
    10261 agtgaaccat tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct
    10321 cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt
    10381 tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga
    10441 ataaaatttg gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca
    10501 caaacccctt gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa
    10561 tagaaatcca tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta
    10621 tggctatggg caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc
    10681 caggcaggga ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag
    10741 agagtggacg ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa
    10801 cggggctcca cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa
    10861 ttgtggagtg ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa
    10921 taagggtgta ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc
    10981 ccacaaaacc actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga
    11041 taggggcgcg attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa
    11101 gcacagggtt gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg
    11161 ccatgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
    11221 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
    11281 gggtagtata tgctatccta atttatatct gggtagcata ggctatccta atctatatct
    11341 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc
    11401 gggtagcata tgctatccta atagagatta gggtagtata tgctatccta atttatatct
    11461 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
    11521 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
    11581 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
    11641 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
    11701 agtatatgct atcctaatct gtatccgggt agcatatgct atcctcatgc atatacagtc
    11761 agcatatgat acccagtagt agagtgggag tgctatcctt tgcatatgcc gccacctccc
    11821 aagggggcgt gaattttcgc tgcttgtcct tttcctgctg gttgctccca ttcttaggtg
    11881 aatttaagga ggccaggcta aagccgtcgc atgtctgatt gctcaccagg taaatgtcgc
    11941 taatgttttc caacgcgaga aggtgttgag cgcggagctg agtgacgtga caacatgggt
    12001 atgcccaatt gccccatgtt gggaggacga aaatggtgac aagacagatg gccagaaata
    12061 caccaacagc acgcatgatg tctactgggg atttattctt tagtgcgggg gaatacacgg
    12121 cttttaatac gattgagggc gtctcctaac aagttacatc actcctgccc ttcctcaccc
    12181 tcatctccat cacctccttc atctccgtca tctccgtcat caccctccgc ggcagcccct
    12241 tccaccatag gtggaaacca gggaggcaaa tctactccat cgtcaaagct gcacacagtc
    12301 accctgatat tgcaggtagg agcgggcttt gtcataacaa ggtccttaat cgcatccttc
    12361 aaaacctcag caaatatatg agtttgtaaa aagaccatga aataacagac aatggactcc
    12421 cttagcgggc caggttgtgg gccgggtcca ggggccattc caaaggggag acgactcaat
    12481 ggtgtaagac gacattgtgg aatagcaagg gcagttcctc gccttaggtt gtaaagggag
    12541 gtcttactac ctccatatac gaacacaccg gcgacccaag ttccttcgtc ggtagtcctt
    12601 tctacgtgac tcctagccag gagagctctt aaaccttctg caatgttctc aaatttcggg
    12661 ttggaacctc cttgaccacg atgctttcca aaccaccctc cttttttgcg cctgcctcca
    12721 tcaccctgac cccggggtcc agtgcttggg ccttctcctg ggtcatctgc ggggccctgc
    12781 tctatcgctc ccgggggcac gtcaggctca ccatctgggc caccttcttg gtggtattca
    12841 aaataatcgg cttcccctac agggtggaaa aatggccttc tacctggagg gggcctgcgc
    12901 ggtggagacc cggatgatga tgactgacta ctgggactcc tgggcctctt ttctccacgt
    12961 ccacgacctc tccccctggc tctttcacga cttccccccc tggctctttc acgtcctcta
    13021 ccccggcggc ctccactacc tcctcgaccc cggcctccac tacctcctcg accccggcct
    13081 ccactgcctc ctcgaccccg gcctccacct cctgctcctg cccctcctgc tcctgcccct
    13141 cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc
    13201 cctcctgctc ctgcccctcc cgctcctgct cctgctcctg ttccaccgtg ggtccctttg
    13261 cagccaatgc aacttggacg tttttggggt ctccggacac catctctatg tcttggccct
    13321 gatcctgagc cgcccggggc tcctggtctt ccgcctcctc gtcctcgtcc tcttccccgt
    13381 cctcgtccat ggttatcacc ccctcttctt tgaggtccac tgccgccgga gccttctggt
    13441 ccagatgtgt ctcccttctc tcctaggcca tttccaggtc ctgtacctgg cccctcgtca
    13501 gacatgattc acactaaaag agatcaatag acatctttat tagacgacgc tcagtgaata
    13561 cagggagtgc agactcctgc cccctccaac agccccccca ccctcatccc cttcatggtc
    13621 gctgtcagac agatccaggt ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc
    13681 accaattact cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc
    13741 ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg atggggattc
    13801 tcgggacccc tcctcttcct cttcaaggtc accagacaga gatgctactg gggcaacgga
    13861 agaaaagctg ggtgcggcct gtgaggatca gcttatcgat gataagctgt caaacatgag
    13921 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
    13981 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
    14041 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
    14101 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
    14161 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
    14221 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
    14281 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
    14341 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
    14401 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
    14461 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
    14521 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
    14581 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
    14641 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
    14701 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
    14761 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
    14821 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
    14881 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
    14941 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
    15001 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
    15061 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    15121 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    15181 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    15241 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    15301 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    15361 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    15421 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    15481 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    15541 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    15601 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    15661 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    15721 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    15781 ctggcctttt gctggccttg aagctgtccc tgatggtcgt catctacctg cctggacagc
    15841 atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag
    15901 gccatccagc ctcgcgtcga attcgtcgac ctcgaaattc taccgggtag gggaggcgct
    15961 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
    16021 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
    16081 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
    16141 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
    16201 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
    16261 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
    16321 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
    16381 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
    16441 ttcgacctgc atccatctag atctcgagca gctgaagctt accatgaccg agtacaagcc
    16501 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
    16561 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
    16621 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
    16681 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
    16741 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
    16801 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
    16861 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
    16921 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
    16981 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
    17041 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
    17101 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg atgatatcag atccccggga
    17161 tgcagaaatt gatgatctat taaacaataa agatgtccac taaaatggaa gtttttcctg
    17221 tcatactttg ttaagaaggg tgagaacaga gtacctacat tttgaatgga aggattggag
    17281 ctacgggggt gggggtgggg tgggattaga taaatgcctg ctctttactg aaggctcttt
    17341 actattgctt tatgataatg tttcatagtt ggatatcata atttaaacaa gcaaaaccaa
    17401 attaagggcc agctcattcc tcccactcat gatctataga tctatagatc tctcgtggga
    17461 tcattgtttt tctcttgatt cccactttgt ggttctaagt actgtggttt ccaaatgtgt
    17521 cagtttcata gcctgaagaa cgagatcagc agcctctgtt ccacatacac ttcattctca
    17581 gtattgtttt gccaagttct aattccatca gaagctggtc gacattggat tggaagtagt
    17641 atactaacct tcacctattg caattctcat gtttgacagc ttatcatcgc agatccgggc
    17701 aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct atacattgaa
    17761 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
    17821 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
    17881 aatatgaccg ccatgtt
//