| 质粒名称: | pAAVLINK-EF1alpha-5'-PLXNB1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001930 |
| 产品用途: | 5' AAVLINK plasmid for PLXNB1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PLXNB1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1alpha-5'-PLXNB1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1alpha-5'-PLXNB1载体质粒基本信息
pAAVLINK-EF1alpha-5'-PLXNB1质粒图谱载体图谱和pAAVLINK-EF1alpha-5'-PLXNB1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1alpha-5'-PLXNB1质粒载体简介
pAAVLINK-EF1alpha-5'-PLXNB1质粒序列载体序列
LOCUS pAAVLINK-EF1alpha-5'-PLXNB1 6343 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6343)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6343
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
promoter 169..711
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 198..409
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 422..690
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 516..521
/label=SDS
CDS 724..3483
/codon_start=1
/gene="PLXNB1"
/gene_synonym="PLEXIN-B1; PLXN5; SEP"
/product="plexin-B1 precursor"
/label=PLXNB1
/note="plexin 5; semaphorin receptor SEP"
/db_xref="GI:194272180"
/db_xref="CCDS:CCDS2765.1"
/db_xref="GeneID:5364"
/db_xref="HGNC:HGNC:9103"
/db_xref="MIM:601053"
/protein_id="NP_001123554.1"
/translation="MPALGPALLQALWAGWVLTLQPLPPTAFTPNGTYLQHLARDPTSG
TLYLGATNFLFQLSPGLQLEATVSTGPVLDSRDCLPPVMPDECPQAQPTNNPNQLLLVS
PGALVVCGSVHQGVCEQRRLGQLEQLLLRPERPGDTQYVAANDPAVSTVGLVAQGLAGE
PLLFVGRGYTSRGVGGGIPPITTRALWPPDPQAAFSYEETAKLAVGRLSEYSHHFVSAF
ARGASAYFLFLRRDLQAQSRAFRAYVSRVCLRDQHYYSYVELPLACEGGRYGLIQAAAV
ATSREVAHGEVLFAAFSSAAPPTVGRPPSAAAGASGASALCAFPLDEVDRLANRTRDAC
YTREGRAEDGTEVAYIEYDVNSDCAQLPVDTLDAYPCGSDHTPSPMASRVPLEATPILE
WPGIQLTAVAVTMEDGHTIAFLGDSQGQLHRVYLGPGSDGHPYSTQSIQQGSAVSRDLT
FDGTFEHLYVMTQSTLLKVPVASCAQHLDCASCLAHRDPYCGWCVLLGRCSRRSECSRG
QGPEQWLWSFQPELGCLQVAAMSPANISREETREVFLSVPDLPPLWPGESYSCHFGEHQ
SPALLTGSGVMCPSPDPSEAPVLPRGADYVSVSVELRFGAVVIAKTSLSFYDCVAVTEL
RPSAQCQACVSSRWGCNWCVWQHLCTHKASCDAGPMVASHQSPLVSPDPPARGGPSPSP
PTAPKALATPAPDTLPVEPGAPSTATASDISPGASPSLLSPWGPWAGSGSISSPGSTGS
PLHEEPSPPSPQNGPGTAVPAPTDFRPSATPEDLLASPLSPSEVAAVPPADPGPEALHP
TVPLDLPPATVPATTFPGAMGSVKPALDWLTREGGELPEADEWTGGDAPAFSTSTLLSG
DGDSAELEGPPAPLILPSSLDYQYDTPGLWELEEATLGASSCPCVESVQ"
misc_feature 1215..1220
/label=SDS
misc_feature 2773..2778
/label=SDS
misc_feature 3324..3329
/label=SDS
intron 3484..3524
/label=Splicing Donor
misc_feature 3528..3561
/label=lox2272-JT1571
repeat_region 3606..3746
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3606..3735
/label=AAV2 ITR
repeat_region 3607..3747
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 3821..4276
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4558..4662
/gene="bla"
/label=AmpR promoter
CDS 4663..5523
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5694..6282
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
721 accatgcctg ctctgggccc agctcttctc caggctctct gggccgggtg ggtcctcacc
781 ctccagcccc ttccaccaac tgcattcact cccaatggca cgtatctgca gcacctggca
841 agggacccca cctcaggcac cctctacctg ggggctacca acttcctgtt ccagctgagc
901 cctgggctgc agctggaggc cacagtgtcc accggccctg tgctagacag cagggactgc
961 ctgccacctg tgatgcctga tgagtgcccc caggcccagc ctaccaacaa cccgaatcag
1021 ctgctcctgg tgagcccagg ggccctggtg gtatgcggga gcgtgcacca gggggtctgt
1081 gaacagcggc gcctggggca gctcgagcag ctgctgctgc ggccagagcg gcctggggac
1141 acacaatatg tggctgccaa tgatcctgcg gtcagcacgg tggggctggt agcccagggc
1201 ttggcagggg agcccctcct gtttgtgggg cgaggataca ccagcagggg tgtggggggt
1261 ggcattccac ccatcacaac ccgggccctg tggccgcccg acccccaagc tgccttctcc
1321 tatgaggaga cagccaagct ggcagtgggc cgcctctccg agtacagcca ccacttcgtg
1381 agtgcctttg cacgtggggc cagcgcctac ttcctgttcc tgcggcggga cctgcaggct
1441 cagtctagag cttttcgtgc ctatgtatct cgagtgtgtc tccgggacca gcactactac
1501 tcctatgtgg agttgcctct ggcctgcgaa ggtggccgct acgggctgat ccaggctgca
1561 gctgtggcca cgtccaggga ggtggcgcat ggggaggtgc tctttgcagc tttctcctcg
1621 gctgcacccc ccactgtggg ccggccccca tcggcggctg ctggggcatc tggagcctct
1681 gccctctgtg ccttccccct ggatgaggtg gaccggcttg ctaatcgcac gcgagatgcc
1741 tgctacaccc gggagggtcg tgctgaggat gggaccgagg tggcctacat cgagtatgat
1801 gtcaattctg actgtgcaca gctgccagtg gacaccctgg atgcttatcc ctgtggctca
1861 gaccacacgc ccagccccat ggccagccgg gtcccgctgg aagccacacc aattctggag
1921 tggccaggga ttcagctaac agctgtggca gtcaccatgg aagatggaca caccatcgct
1981 ttcctgggtg atagtcaagg gcagctgcac agggtctact tgggcccagg gagcgatggc
2041 cacccatact ccacacagag catccagcag gggtctgcag tgagcagaga cctcaccttt
2101 gatgggacct ttgagcacct gtatgtcatg acccagagca cacttctgaa ggttcctgtg
2161 gcttcctgtg ctcagcacct ggactgtgca tcttgccttg ctcacaggga cccatactgt
2221 gggtggtgcg tgctccttgg caggtgcagt cgccgttctg agtgctcgag gggccagggc
2281 ccagagcagt ggctatggag cttccagcct gagctgggct gtctgcaagt ggcagccatg
2341 agtcctgcca acatcagccg agaggagacg agggaggttt tcctatcagt gccagacctg
2401 ccacccctgt ggccagggga gtcatattcc tgccactttg gggaacatca gagtcctgcc
2461 ctgctgactg gttctggtgt gatgtgcccc tccccagacc ctagtgaggc cccagtgctg
2521 ccgagaggag ccgactacgt atccgtgagc gtggagctca gatttggcgc tgttgtgatc
2581 gccaaaactt ccctctcttt ctatgactgt gtggcggtca ctgaactccg cccatctgcg
2641 cagtgccagg cctgtgtgag cagccgctgg gggtgtaact ggtgtgtctg gcagcacctg
2701 tgcacccaca aggcctcgtg tgatgctggg cccatggttg caagccatca gagcccgctt
2761 gtctccccag accctcctgc aagaggtgga cccagcccct ccccacccac agcccccaaa
2821 gccctggcca cccctgctcc tgacaccctt cccgtggagc ctggggctcc ctccacagcc
2881 acagcttcgg acatctcacc tggggctagt ccttccctgc tcagcccctg ggggccatgg
2941 gcaggttctg gctccatatc ttcccctggc tccacagggt cgcctctcca tgaggagccc
3001 tcccctccca gcccccaaaa tggacctgga accgctgtcc ctgcccccac tgacttcaga
3061 ccctcagcca cacctgagga cctcttggcc tccccgctgt caccctcaga ggtagcagca
3121 gtgccccctg cagaccctgg ccccgaggct cttcatccca cagtgcccct ggacctgccc
3181 cctgccactg ttcctgccac cactttccca ggggccatgg gctccgtgaa gcccgccctg
3241 gactggctca cgagagaagg cggcgagctg cccgaggcgg acgagtggac ggggggtgac
3301 gcacccgcct tctccacttc caccctcctc tcaggtgatg gagactcagc agagcttgag
3361 ggccctcccg cccccctcat cctcccgtcc agcctcgact accagtatga cacccccggg
3421 ctctgggagc tggaagaggc gaccttgggg gcaagctcct gcccctgtgt ggagagcgtt
3481 caggtaagta tcaaggttac aagacaggtt taaggagacc aataagctat tattcgtata
3541 ggatacttta tacgaagtta ttaagttatg gagaccggtg tggcacgtgc ggaccgagcg
3601 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
3661 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
3721 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct
3781 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
3841 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
3901 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
3961 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
4021 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
4081 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
4141 acaacactca accctatctc gggctattct tttgatttat aagggatttt gccgatttcg
4201 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
4261 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
4321 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
4381 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
4441 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
4501 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
4561 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
4621 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
4681 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
4741 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
4801 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
4861 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
4921 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
4981 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
5041 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
5101 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
5161 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
5221 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
5281 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
5341 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
5401 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
5461 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
5521 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
5581 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
5641 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
5701 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
5761 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
5821 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
5881 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
5941 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
6001 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
6061 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
6121 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
6181 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
6241 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
6301 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
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