pLIX_403

LOCUS       pLIX_403              9396 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA.
ACCESSION   .
VERSION     .
KEYWORDS    pLIX_403
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9396)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9396
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    547..671
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        696..726
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             780..1439
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(846..865)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1781..2086
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1965..1984
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2127..2251)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2256..2297
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2311..2328
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2338..2848
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2767..2785
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2858..3454
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2858..2877)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3354..3374
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3455..3508
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3509..4255
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4295..4315)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4713..4724)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4929..5162
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5234..5355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5271..5290)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5325..5344
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5395..5530
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5457..5476
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5530..5601
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5602..6462
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5820..5839)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6633..7221
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7122..7141
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7375..7392
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7509..7530
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7545..7575
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7583..7599
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7588..7610
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7607..7623
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7607..7623
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7642..7662
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7644..7662
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7690..7916
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7917..8097
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8144..8269
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8762..8995
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
      601 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
      661 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
      721 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
      781 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
      841 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
      901 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
      961 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     1021 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     1081 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     1141 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     1201 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     1261 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     1321 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     1381 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     1441 gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
     1501 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
     1561 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
     1621 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
     1681 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
     1741 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
     1801 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     1861 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     1921 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     1981 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     2041 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
     2101 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
     2161 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     2221 tttctcgttc agctttcttg tacaaagtgg ttgcaggaaa gccaatccca aacccactcc
     2281 taggtctcga cagtacagca tagaccggtc caccaccacc accaccacta aggatccggg
     2341 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
     2401 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
     2461 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
     2521 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
     2581 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
     2641 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
     2701 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
     2761 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
     2821 tgaccgaatc accgacctct ctccccagca attcaccatg accgagtaca agcccacggt
     2881 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     2941 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     3001 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     3061 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     3121 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     3181 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     3241 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     3301 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     3361 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     3421 gcgcacctgg tgcatgaccc gcaagcccgg tgccgaaggt agaggttctc tcctcacttg
     3481 tggtgatgtt gaagaaaacc ctggtccaat gtctagactg gacaagagca aagtcataaa
     3541 cggagctctg gaattactca atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc
     3601 tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct
     3661 gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga
     3721 aggcgagtca tggcaagact ttctgcggaa caacgccaag tcataccgct gtgctctcct
     3781 ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga
     3841 aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact
     3901 gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca
     3961 tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag
     4021 acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga
     4081 actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc
     4141 ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga
     4201 tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaactaag
     4261 taaggatcga tccaagatat cgtattctta actatgttgc tccttttacg ctatgtggat
     4321 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4381 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4441 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4501 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4561 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4621 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     4681 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     4741 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     4801 gtcggatctc cctttgggcc gcctccccgc ctgtttcgcc tcggcgtccg gactagaggt
     4861 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     4921 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     4981 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5041 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5101 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5161 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     5221 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5281 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5341 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     5401 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5461 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     5521 tttggaggcc tttcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5581 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5641 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5701 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5761 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5821 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     5881 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5941 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6001 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6061 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6121 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6181 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6241 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6301 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6361 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6421 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6481 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6541 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6601 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6661 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6721 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6781 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6841 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6901 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6961 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7021 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7081 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7141 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7201 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7261 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7321 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     7381 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     7441 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     7501 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     7561 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     7621 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     7681 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
     7741 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
     7801 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
     7861 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
     7921 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7981 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8041 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     8101 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     8161 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     8221 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     8281 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     8341 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     8401 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     8461 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     8521 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     8581 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     8641 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     8701 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     8761 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     8821 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     8881 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     8941 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     9001 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     9061 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     9121 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     9181 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     9241 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     9301 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     9361 tatcgtttca gacccacctc ccaaccccga ggggac
//