| 质粒名称: | p5shell |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 46953 |
| 存储实验室: | Vladislav Verkhusha |
|
载体骨架基本信息 |
|
| 载体名称: | p5sheLL |
| 载体出品公司: | Clontech |
| 空载体大小: | 4038 bp |
| 完整质粒大小: | 4989 bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | iRFP713 |
| 物种来源: | 光合细菌 Rhodopseudomonas palustris |
| 插入大小: | 951 bp |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AgeI(未破坏) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | CMV-F |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | |
| 参考文献部分: | |
买家导航
p5shell载体质粒基本信息
p5shell质粒图谱载体图谱和p5shell载体序列质粒序列多克隆位点信息
p5shell质粒载体简介
p5shell质粒序列载体序列
LOCUS p5sheLL 10970 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION encode HPV5 L1 and L2, Bicistronic, too large to self-package.
ACCESSION .
VERSION .
KEYWORDS p5sheLL
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10970)
AUTHORS Buck CB, Thompson CD, Roberts JN, Muller M, Lowy DR, Schiller JT
TITLE Carrageenan is a potent inhibitor of papillomavirus infection.
JOURNAL PLoS Pathog. 2006 Jul;2(7):e69.
PUBMED 16839203
REFERENCE 2 (bases 1 to 10970)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10970
/organism="synthetic DNA construct"
/mol_type="other DNA"
regulatory 17..26
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
protein_bind complement(1581..1605)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
regulatory 2278..2287
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
misc_feature 3907..4495
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3960..3980)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4378..4389)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4532..4549)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4538..4762
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4808..5236
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4895..4914)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5105..5126
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5245..5265)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
CDS 7194..7562
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="KLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVT
LFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREF
ALRDPAGNCVHFVAEEQD"
polyA_signal 7692..7813
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7729..7748)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7783..7802
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7862..7878)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7862..7878)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7875..7897)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7886..7902
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7910..7940)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7955..7976
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8093..8110)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8264..8849)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8344..8363)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9020..9880)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9643..9662
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9881..9985)
/gene="bla"
/label=AmpR promoter
primer_bind complement(10060..10079)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 10251..10630
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 10631..10834
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 10785..10805
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 10836..10854
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 10857..10875
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 10885..10909
/label=LNCX
/note="Human CMV promoter, forward primer"
ORIGIN
1 tccggagggc cctagagcca ccatggccgt ctggcatagc gccaacggca aggtctactt
61 gcccccgagc acccccgtcg cacgcgtgca gtctacagac gagtatatcc agcgcaccaa
121 catctactac cacgccttct ccgatcgcct cctgaccgtg ggccacccct actttaacgt
181 gtataacatc aacggcgaca agttggaagt ccccaaagtc agcggcaacc agcatcgcgt
241 gttcaggttg aagctgcccg accccaatcg cttcgccctg gccgacatga gcgtctacaa
301 tcccgataag gagaggctcg tctgggcatg ccgcgggctg gagatcggcc gcgggcaacc
361 cctgggcgtg ggctccaccg gccatcccta ctttaacaag gtcaaggaca ccgagaattc
421 caacgcctat atcaccttca gcaaggacga tcgccaagac accagcttcg accccaagca
481 gattcagatg ttcatcgtgg gctgtacccc ctgtatcggc gaacactggg acaaggccgt
541 cccctgcgcc gagaacgacc aacagaccgg gttgtgcccc cccatcgagt tgaagaatac
601 ctacatcgag gacggcgaca tggccgatat cggcttcggc aatatgaact tcaaagcgtt
661 gcaggactcc cggagcgacg tgtccctgga tattgtgaac gagacctgca agtaccccga
721 cttcctgaaa atgcagaatg acatctacgg ggacgcctgt ttcttctacg ccaggcgcga
781 acagtgctac gcacgccatt tcttcgtccg cggcggcaag accggcgacg atatccccgg
841 cgcccagatc gataacggca cctataagaa ccaattctat atccccggcg ccgacgggca
901 ggcccagaaa accatcggca acagtatgta ctttcccacc gtctccggga gcctggtcag
961 ttccgacgcc cagctcttca atcgcccatt ttggttgcag cgcgcacagg gccacaacaa
1021 cgggattctc tgggccaacc agatgttcat taccgtcgtc gataacaccc gcaacaccaa
1081 cttttccatc agcgtgtaca accaagccgg cgccttgaag gacgtcgccg attacaacgc
1141 cgaccagttc cgcgagtacc agcgccacgt ggaggagtac gagatcagcc tgatcttgca
1201 gttgtgcaaa gtccccctga aggccgaagt gctcgcccag attaacgcca tgaatagcag
1261 cctgctcgaa gactggcagc tgggcttcgt cccaaccccc gacaacccca tccaagatac
1321 atatcgctac atcgatagcc tcgccaccag atgccccgac aagaaccccc ccaaggagaa
1381 ggaggatccc tacaaagggc tgcacttctg ggacgtggac ctgaccgagc gcctcagcct
1441 ggacctggac cagtacagtc tggggcgcaa gttcctgttt caggccggcc tgcagcagac
1501 cacagtcaat ggcaccaagg ccgtcagcta caagggcagc aaccgcggca ccaagaggaa
1561 gaggaagaac tgagcccggg acccagcttt cttgtacaaa gtggttcgat ctagaatggc
1621 tagggctcga ggaagcttaa aacagctctg gggttgtacc caccccagag gcccacgtgg
1681 cggctagtac tccggtattg cggtaccctt gtacgcctgt tttatactcc cttcccgtaa
1741 cttagacgca caaaaccaag ttcaatagaa gggggtacaa accagtacca ccacgaacaa
1801 gcacttctgt ttccccggtg atgtcgtata gactgcttgc gtggttgaaa gcgacggatc
1861 cgttatccgc ttatgtactt cgagaagccc agtaccacct cggaatcttc gatgcgttgc
1921 gctcagcact caaccccaga gtgtagctta ggctgatgag tctggacatc cctcaccggt
1981 gacggtggtc caggctgcgt tggcggccta cctatggcta acgccatggg acgctagttg
2041 tgaacaaggt gtgaagagcc tattgagcta cataagaatc ctccggcccc tgaatgcggc
2101 taatcccaac ctcggagcag gtggtcacaa accagtgatt ggcctgtcgt aacgcgcaag
2161 tccgtggcgg aaccgactac tttgggtgtc cgtgtttcct tttattttat tgtggctgct
2221 tatggtgaca atcacagatt gttatcataa agcgaattgg attgcggccg ctctagagcc
2281 accatggcca gggccaagcg cgtgaaaagg gatagcgtga cccacatcta tcagacatgc
2341 aagcaagccg ggacctgtcc acccgacgtc atcaacaagg tcgagcagac caccgtcgcc
2401 gataacatcc tgaagtacgg gtccgccggc gtgttcttcg gcgggttggg catctccacc
2461 gggaggggca ccggcggcgc caccggctat gtccccttgg gcgagggccc cggcgtcagg
2521 gtgggcggca caccaaccgt cgtgcgcccc agtctcgtcc ccgagaccat tggcccagtc
2581 gacatcctcc caatcgacac cgtcaatcca gtcgagccca ccgccagcag tgtcgtgccc
2641 ttgaccgaaa gtaccggggc cgacctgttg cccggcgagg tcgagaccat cgccgagatt
2701 caccccgtgc ccgagggccc cagcgtcgac acacccgtgg tcacaacctc aaccggcagt
2761 tccgccgtcc tggaagtcgc acccgagccc atcccaccca ccagagtgcg cgtcagcagg
2821 acccaatacc ataaccccag cttccagatc atcaccgaaa gcacccccgc ccagggcgag
2881 agcagcttgg ccgaccatgt cctcgtcacc agcggcagcg gcggccagag gatcggcggc
2941 gacatcaccg atatcatcga gctggaagag atcccatccc gctacacctt cgagatcgag
3001 gagcccaccc caccgaggag gtcatccacc cccctgccga ggaaccagag cgtggggagg
3061 cgccgcggct ttagcctcac caaccgcagg ctggtgcagc aagtgcaggt cgataacccg
3121 ctgttcttga cccagcccag caaactggtc aggttcgcct tcgacaaccc cgtcttcgaa
3181 gaggaggtca ccaacatctt cgagaacgac ctcgacgtgt tcgaggagcc ccccgatcgc
3241 gacttcttgg acgtccgcga gctcggcagg ccccagtaca gcaccacccc cgccggctac
3301 gtccgcgtgt cacgcctcgg caccagggca accatcagga ccaggagcgg cgcccaaatc
3361 ggcagccagg tccacttcta tcgcgacttg tctagcatca acaccgagga ccccatcgag
3421 ctgcagctgc tggggcagca cagcggcgac gccaccatcg tgcagggccc cgtcgagtca
3481 accttcatcg acatggacat cagcgagaac cccctgagcg agtctatcga ggcctacagc
3541 cacgacctgc tgctggacga gaccgtcgag gacttttccg gcagccaact cgtcatcggc
3601 aacaggcgct caaccaatag ctataccgtc ccccgcttcg agaccacccg caacggcagc
3661 tactacaccc aggataccaa aggctactac gtcgcctacc ccgaaagcag gaacaacgcc
3721 gagatcatct accccacccc cgacatcccc gtcgtgatca tccatcccca cgattccacc
3781 ggcgatttct acctgcaccc atccttgagg cgcaggaaga ggaagcgcaa gtacctctga
3841 gcccgggacc cagctttctt gtaccacgcg tgaattctcg aggctagcga tatcaagctt
3901 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
3961 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
4021 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
4081 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
4141 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
4201 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
4261 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
4321 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
4381 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
4441 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
4501 taccgtcggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
4561 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
4621 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
4681 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
4741 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
4801 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
4861 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
4921 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
4981 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
5041 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
5101 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
5161 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
5221 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
5281 gctccccagc aggcagaagt atgcaaagca tgcagaattc tatcaaatat ttaaagaaaa
5341 aaaaattgta tcaactttct acaatctctt tcagaagaca gaagcagagg gaatacttcc
5401 taaatcattc aactaggcca gcattacctt aataccggaa ctagaaaatg acattacaag
5461 aaaagaaaac aacagaccaa tatctctcat gaacaaagat acaaacattt tcaacaaaat
5521 attagcaaaa agaatccaag aatgtatcaa aaaatataca ccacaaccaa gtagaattta
5581 ttccagatat gtaagggtgg ttcaacgttt gaaaatcaat taacgtaatt tgtcccatca
5641 acaggttaaa gaagaaaatc acatggtcat attgatagac acagaaaaag catttgacaa
5701 aatttaacac ccattcatga tgcaatctct cagtaaacta ggaatagagg aaaacttcct
5761 cagcttgaat gtaccttcct ctcaattttg ctatgaacct gaaactcctc ttaaaaaata
5821 aagtttttca tttaaaaaga aaacaaaaaa catggaggag cgttgatgta tctcatttta
5881 gaccaatcag ctatggatag ttaggcgaca gcacagatag ctgctgtact tctgtttctg
5941 gcaatgttcc agactacatt taaaaaattt ttaattatag acttgtactt aatgttcaag
6001 aaaaatatga aaatggcttt gccgtgttaa tgctactctt ttttaaaaaa aactaaagtt
6061 caaactttat ttatatttca ttagtttttt agctactgtt ctttttctgt tctgggatct
6121 cattcagaat gccacattac atataattct catgtctcct tgggttcctc ttagttttga
6181 cagttcctca gacttttctt atttttgatg accttgacag ttttgaggag tactggttag
6241 atatagggta atggttttta aagtatattt gtcatgattt atactggggt aagggtttgg
6301 ggaggaagcc catggggtaa agtactgttc tcatcacatc atatcaaggt tatataccat
6361 caatattgcc acagatgtta cttagccttt taatatttct ctaatttagt gtatatgcaa
6421 tgatagttct ctgatttctg agattgagtt tctcatgtgt aatgattatt tagagtttct
6481 ctttcatctg ttcaaatttt tgtctagttt tattttttac tgatttgtaa gacttctttt
6541 tataatctgc atattacaat tctctttact ggggtgttgc aaatattttc tgtcattcta
6601 tggcctgact tttcttaatg gttttttaat tttaaaaata agtcttaata ttcatgcaat
6661 ctaattaaca atcttttctt tgtggttagg actttgagtc ataagaaatt tttctctaca
6721 ctgaagtcat gatggcatgc ttctatatta ttttctaaaa gatttaaagt tttgccttct
6781 ccatttagac ttataattca ctggaatttt tttgtgtgta tggtatgaca tatgggttcc
6841 cttttatttt ttacatataa atatatttcc ctgtttttct aaaaaagaaa aagatcatca
6901 ttttcccatt gtaaaatgcc atattttttt cataggtcac ttacatatat caatgggtct
6961 gtttctgagc tctactctat tttatcagcc tcactgtcta tccccacaca tctcatgctt
7021 tgctctaaat cttgatattt agtggaacat tctttcccat tttgttctac aagaatattt
7081 ttgttattgt cttttgggct tctatataca ttttagaatg aggttggcaa gttaacaaac
7141 agcttttttg gggtgaacat attgactaca aatttatgtg gaaagaaagt accaagttga
7201 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
7261 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
7321 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
7381 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
7441 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
7501 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
7561 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
7621 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
7681 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
7741 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
7801 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
7861 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
7921 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
7981 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
8041 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
8101 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
8161 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
8221 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
8281 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
8341 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
8401 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
8461 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
8521 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
8581 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
8641 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
8701 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
8761 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc
8821 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
8881 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
8941 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
9001 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
9061 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
9121 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
9181 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
9241 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
9301 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
9361 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
9421 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
9481 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
9541 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
9601 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
9661 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
9721 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
9781 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
9841 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
9901 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
9961 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
10021 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
10081 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
10141 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
10201 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
10261 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
10321 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
10381 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
10441 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
10501 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
10561 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
10621 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
10681 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggaaccaaaa
10741 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
10801 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
10861 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
10921 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
//
