pCDH-PCaMKII-NLS-mTagBFP2

LOCUS       pCDH-PCaMKII-NLS-mTagBFP2                7464 bp DNA     circular SYN 02-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    2492- pCDH-PCaMKII-NLS-mTagBFP2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 1, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     28..44
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        59..285
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             286..466
                     /label=5' LTR
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    513..638
                     /label=HIV-1 psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1131..1364
                     /label=HIV-1 RRE
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
     misc_feature    1860..1976
                     /label=HIV-1 cPPT
                     /note="central polypurine tract from pol gene of human 
                     immunodeficiency virus type 1"
     promoter        1989..2359
                     /label=alpha-CamKII Promotor Truncated
     intron          2366..2467
                     /label=Mouse Insulin Intron 1-2
     primer_bind     2470..2534
                     /label=NLSB F EcoR1
     CDS             2529..2555
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             2556..2615
                     /codon_start=1
                     /product="two tandem HA epitope tags"
                     /label=2xHA
                     /translation="YPYDVPDYAGYPYDVPDYAG"
     CDS             2622..3332
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(2785..2801)
                     /label=BFP_R
     primer_bind     complement(3314..3332)
                     /label=NLSB R Sal1
     primer_bind     complement(3569..3595)
                     /label=NLSB R Sal1
     misc_feature    3578..4166
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
     CDS             complement(4049..4060)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4240..4473
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4545..4666
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4706..4841
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4874..4890)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4898..4914
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4922..4952)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4967..4988
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5276..5864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6035..6895)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6896..7000)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctgacgcgtg
       61 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
      121 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
      181 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
      241 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
      301 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      361 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      421 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
      481 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
      541 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
      601 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
      661 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
      721 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
      781 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
      841 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
      901 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
      961 ccaccgcaca gcaagcggcc actgatcttc agacctggag gaggagatat gagggacaat
     1021 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     1081 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     1141 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     1201 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     1261 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     1321 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     1381 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     1441 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     1501 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     1561 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     1621 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     1681 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     1741 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     1801 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     1861 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     1921 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     1981 atactagtac ttgtggacta agtttgttca catccccttc tccaaccccc tcagtacatc
     2041 accctgggag aacaaggtcc acttgcttct gggcccacac agtcctgcag tattgtgtat
     2101 ataaggccag ggcaacggag gagcaggttt tgaagtgaaa ggcaggcagg tgttggggag
     2161 gcagttaccg gggcaacggg aacagggcgt ttcggaggtg gttgccatgg ggacctggat
     2221 gctgacgaag gctcgcgagg ctgtgagcag ccacagtgcc ctgctcagaa gccccaagct
     2281 cgtcaatcaa gctggttctc catttgcact caggagcacg ggcaggcgag tggcccctag
     2341 ttctgggagc agagtatcag atatcgtact cttctcagtg ggcctggctc cccagctaag
     2401 acctcaggga cttgaggtag gatatagcct cctctcttac gtgaaacttt tgctatcctc
     2461 aacccaggat atctctagtt gatcagaatt caccggtatg catatttaaa tgaagcgctg
     2521 ccaccatgcc agcggcgaaa agagtaaaac tggactaccc atacgatgtt cctgactatg
     2581 cgggctatcc gtatgacgtc ccggactatg caggactcga gatggtgtct aagggcgaag
     2641 agctgattaa ggagaacatg cacatgaagc tatacatgga gggcaccgtg gacaaccatc
     2701 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
     2761 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc
     2821 tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt
     2881 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
     2941 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
     3001 gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
     3061 tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga
     3121 agctcgtggg cgggagccat ctgatcgcaa acgccaagac cacatataga tccaagaaac
     3181 ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca
     3241 aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg
     3301 acctccctag caaactgggg cacaagctca atctcgagtc tagagcggcc gcaggatccg
     3361 tttaaacacc ggttgtacaa gtgagggccc catgttcgcc ttccgccgcg tggaggagct
     3421 gcacagcaac accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc
     3481 cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg
     3541 ctccaccgga tctcgctaga gctgaatcta agtcgacaat caacctctgg attacaaaat
     3601 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     3661 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     3721 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     3781 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     3841 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     3901 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     3961 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     4021 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4081 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4141 gatctccctt tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag
     4201 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     4261 aacgaagata agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     4321 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     4381 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     4441 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     4501 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     4561 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4621 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     4681 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4741 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4801 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca
     4861 aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     4921 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     4981 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5041 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5101 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5161 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5221 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5281 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5341 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5401 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5461 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5521 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5581 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5641 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5701 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5761 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5821 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5881 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5941 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6001 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6061 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6121 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6181 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6241 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     6301 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     6361 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     6421 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     6481 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6541 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6601 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     6661 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     6721 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     6781 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6841 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6901 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6961 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7021 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     7081 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     7141 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     7201 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     7261 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     7321 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     7381 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     7441 gggtaacgcc agggttttcc agtc
//