| 质粒名称: | CaV1.2,pCDNA6-V5-His-CaV1.2-m |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 26572 |
| 存储实验室: | Diane Lipscombe |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA6/V5-His |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5100 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Calcium channel, voltage-dependent, L type, alpha 1C subunit |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 6600 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | KpnI（销毁后未知） |
| 3' 克隆位点: | NotI（销毁后未知） |
| 5' 测序引物 | T7 |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | CaV1.2 was a gift from Diane Lipscombe (Addgene plasmid # 26572 ; http://n2t.net/addgene:26572 ; RRID:Addgene_26572) |
| 参考文献部分: | Neuronal L-type calcium channels open quickly and are inhibited slowly. Helton TD, Xu W, Lipscombe D. J Neurosci. 2005 Nov 2. 25(44):10247-51. 10.1523/JNEUROSCI.1089-05.2005 PubMed 16267232 |
买家导航
CaV1.2载体质粒基本信息
CaV1.2质粒图谱载体图谱和CaV1.2载体序列质粒序列多克隆位点信息
CaV1.2质粒载体简介
CaV1.2质粒序列载体序列
LOCUS CaV1.2 11709 bp ds-DNA circular SYN 09-APR-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS CaV1.2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11709)
AUTHORS Helton TD, Xu W, Lipscombe D
TITLE Neuronal L-type calcium channels open quickly and are inhibited
slowly.
JOURNAL J Neurosci. 2005 Nov 2. 25(44):10247-51.
PUBMED 16267232
REFERENCE 2 (bases 1 to 11709)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 9, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11709
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 107..128
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 186..208
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 205..221
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
polyA_signal 270..391
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(281..300)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 335..354
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
CDS complement(549..947)
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
promoter complement(966..1013)
/label=EM7 promoter
/note="synthetic bacterial promoter "
promoter complement(1061..1257)
/label=SV40 promoter
/note="SV40 early promoter"
promoter complement(1069..1279)
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 1075..1210
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(1129..1148)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 1303..1323
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin complement(1332..1760)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1442..1463)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 1654..1673
/label=F1ori-R
/note="F1 origin, reverse primer"
polyA_signal 1806..2030
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind 2019..2036
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
CDS complement(2059..2076)
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS complement(2086..2127)
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
RBS 6340..6351
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
primer_bind complement(8891..8910)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(8892..8910)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(8955..9158)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(8984..9004)
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
enhancer 9159..9538
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind 9710..9729
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 9804..9908
/gene="bla"
/label=AmpR promoter
CDS 9909..10769
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(10127..10146)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 10940..11528
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 11429..11448
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 11682..11699
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct
241 agctagaggt cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac
301 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
361 tgtaaccatt ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat
421 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
481 catagaaggc ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca
541 cgaagtgctt agccctccca cacataacca gagggcagca attcacgaat cccaactgcc
601 gtcggctgtc catcactgtc cttcactatg gctttgatcc caggatgcag atcgagaagc
661 acctgtcggc accgtccgca ggggctcaag atgcccctgt tctcatttcc gatcgcgacg
721 atacaagtca ggttgccagc tgccgcagca gcagcagtgc ccagcaccac gagttctgca
781 caaggtcccc cagtaaaatg atatacattg acaccagtga agatgcggcc gtcgctagag
841 agagctgcgc tggcgacgct gtagtcttca gagatgggga tgctgttgat tgtagccgtt
901 gctctttcaa tgagggtgga ttcttcttga gacaaaggct tggccatggt ttagttcctc
961 accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc aacacgtgct
1021 gatcagatcc gaaaatggat atacaagctc ccgggagctt tttgcaaaag cctaggcctc
1081 caaaaaagcc tcctcactac ttctggaata gctcagaggc agaggcggcc tcggcctctg
1141 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
1201 ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc
1261 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat
1321 tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
1381 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
1441 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
1501 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
1561 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
1621 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
1681 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
1741 tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc tttccgcctc
1801 agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc
1861 cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga
1921 tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga
1981 aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcgaggctga
2041 tcagcgggtt taaactcaat ggtgatggtg atgatgaccg gtacgcgtag aatcgagacc
2101 gaggagaggg ttagggatag gcttaccttc gaagggccct ctagactcga gcggccgcca
2161 gtgtgatgga tatctgcaga attcggcttg cttatcccga cagcagtgaa tgaagacgct
2221 gttccggtta cctccaggtc acgagaacag tgaggcactt ctgaaacgaa cccattagga
2281 acattgaaac gaataaaaaa accacccgcg tctcgccagc cctgaggact acaggttgct
2341 gacgtaggac ctgctgtccg cgagcgcctc ctcgctccgg cctcgcccca gggcatatgc
2401 gcagctctcg tcctctggga ccacagccct gtcctgcccc gggtccctgc agttcacaaa
2461 aggtaagagg gtgccgttgg ggctctgctg ggcgccccca ctgaggatgt tgtctgcggc
2521 gttctccatc tcctctattg tcatgtcgca ggcgtcagcc agctcctggg tggtgacctc
2581 gataaacttg ggatcttgag caaactgtcc cagtccttct gaaatcaaga ccgcttccac
2641 caggctgctg gcactgccat ggaactgtct ccctggagct ccagcctggc tgggcacggt
2701 gagggagacg ggccgggctc tccgggccat gctgctgctc ccactacagg ctgtcgtctc
2761 ctcagaccag gagctgcagt ggatggatgg gaagctgctg ttgagtttct cgctggactc
2821 tgccccctcc agccgtaggg tagggatggg ccgtaggggt ctgccccggc tgcctggagt
2881 gacagggggt gtggggcatg gcctggggaa tgtggtagga gaatggcttc tctgcaggag
2941 ggggctcaag cctgccactg ccaatgcctg atgatgaacc agatgcaagg gcaaggctgt
3001 cttctgagag atgtctcccc cttgatcctt ttgtcgcttt agacattcca gatggaagga
3061 ggcccttcga cctagagagg cagagcgaag gaaactcctc tttggagatg gctggatctc
3121 cctcttgtcc tcctctgggc aggtcagttg tcggtgttca tcttcctggt aggagaacat
3181 atctgtggag agcaggctgg gctcactgca gtactcggct tcttcactca tccttacgct
3241 gcgggtctca tctggaaata tctcctgatt caccgtggct ccctggctct ctcgggagtg
3301 gcacctctta gagctgagct tccatgctgc ctcctgtact cggacagcag gggacaaggg
3361 aggtccatgg ccctccacag tgctgaccgt gctggagtag ccagcgggat gggggaagcg
3421 gcccagggca gtgttgttgg cattgttgat gttggcattg gagcccgtgg atgagtagct
3481 gctgggggtg aacgtggagt ccaccagctt ctcatgggac ggtgactcag tgtcagcttg
3541 gttgttccct gtcttgttga tgtgcagtgg gcgctgagtg gcgaacgtct gaggaaagtt
3601 gcccctgctg tcactctgat agtaggtgac atggttgccg aacaggcctc cagccctcct
3661 gaagatgtca tcttcggagg cagcagacac cgcctccttc atagccttgt ccaactcctc
3721 ctcagcagtc agatccccag agatggcccg ccggatctca ggcccaatat catgcaaggt
3781 gcgcaagcca gcctggaggg acagtgcatt cctttgtgag ggcttgccca ccagcccctg
3841 ctcttttcgc ttcttgaatt tcctgaagta ctcttgtatc aggaaggtgg catagaactt
3901 gcccactgtg acctcgtcat cgcctgcagg gggcaccacc tggtccaaca gcttcatgct
3961 ggtcctcttc cagattttct tgatgatggc ccgcagctcc tcattggctt gctctaggtt
4021 cccttctgtt ttgatcctca gggctgtcct gacgagggca aacagggtag cattgaacat
4081 gactgtgcca tcgctgttca gaggcatgtt catggacacc aggcgtttgc aggccacacg
4141 gtgaggacac aatttcccaa aacccagtgg gggctgaatt cgacggagga gggtcaccac
4201 atccaagtgt ttgatccgac ccttggcttc agggtcatac tcggcccaga ttctcttgaa
4261 ttcatccagg tgatggggac cgaggataga ccaatcccta gtcaggtagt caaagttgtc
4321 catgataaca gctacaaaga ggttgatgat caggaaggca cagagcatgt agaagctgat
4381 gaagtagaag acagcaaagc tgctgccaca gggggtctcc ccttccgtgc tgttgctggg
4441 ctcagactct ggggcacact tcttgcctgg catgcaggcc agcatgatgt cctgccaggc
4501 ctctccggtg gcacacctga acagcagtaa cacagcctgg gggaacgtct ggaagttgtt
4561 gttccgattg atctctgtgg tgtcattcag ggcaatcttc ccaaacacct gcatcccaat
4621 cactgcatag ataaagaaca gcatcacaat caaaagagcc acatagggca gagcctggaa
4681 ggacttgatg aaggtccaca gcagggttcg gatgccttcc ccgcggctca gcagcttcac
4741 caggcgcatg acccggaaga ggcggaagaa ggtgatggag atgcgggagt tctcctctgc
4801 actcatagag ggagagcatt gggtatgttc agctgggtgt acctcggtga ttgctatatc
4861 aacaatgcta cccacaacaa tcaaggcgtc aaatgtattc catgcatcac agaaatagtg
4921 cttgggtttg aaggcaatga gcttcaggat catctccact gtgaagaggc cggtgaaaag
4981 catgttgagt atattcatgg cgattttgaa gaggcagctc tggccatagt gctgcatggc
5041 caggcagatg gtgttgagca ggatgagaac gaacatcaga tactcgaagt aggtagagtt
5101 gaccacgtac cacactttgt actggtgctg gttcttgggg atgtaccttc gcaagggtcg
5161 ggccttgagg gcatattcca cacattgtct ctggttcttg tccagctcac agttcttgta
5221 ctcttgttcc ccctgctcct ggaaggtgac aatgacgaaa cccacgaaga tgttcatcat
5281 gaagaaggca atgatgatga tatagatgat gaagaagatg gagatctcca cacggtagtt
5341 gtagatgggg cccttgtctt ctgtgtggga gtcaatggag cggtacagca gctctggcca
5401 cccttcgaag gtggagacgg tgaagagagc catcatggct gccaaaacat tgtcaaagtc
5461 aaacttgctg ttctcccagc ttcgaggctg gataatgggg tgatcgacct ctccatcttt
5521 gtatgtgata tagttaccct tgcattctgc ctccgtctgt ttagaactat ccgaacaggt
5581 atagagcttt cccttgaaga gctggacccc aatgcaggcg aacatgaact gcagcagagt
5641 ggtgacaatt acgatgttcc cgatggtccg gatggccaca aacacgcact gaaccacatg
5701 ctttagcccc ttggccctgt tgatggccct caggggtctg aggactcgca gcactcgtaa
5761 aatcttcaca acgttgatcg cgctggactg gatgccaaag gagatgaggg acacgctaac
5821 caccagcagg tccaggatat tgaagtagtt tcggcagaaa gagcccttgt gcaggaaagc
5881 cccgtaagca gtcatcttaa ggataatttc taatgtaaag atactagtga agacatagtc
5941 tgcattgcct aggatatggt tcctgaagga ggtgtgctgg acggggtcct cagcagccag
6001 agagatgctg ctgagcagaa tgaagaagag gatgaggttg gtgaagatcg tgtcattgac
6061 aatacggtgg cactgcaggc ggaacctgtt gtttgggctg aagatgaaaa atgcactggc
6121 ttccggcatg ggaactgcct tttccttaag gtgcagctca gacaggggcc gggggcgtgg
6181 ccccacaggc atctctggct cctcttcatc ctcttcccct gcagtgtctg ggttggagtg
6241 gggactctta tcctcgtttt cactgggctg gaggtcatcc atgttgatct tggtagtggg
6301 tggggattct ccatcggctg taatggattt cagttcaatt ttctcctctt tgctctcctc
6361 cacggctggc ttctccatca cctcctgttt cttttctggg ctggcagtcc tggccagctt
6421 cttcctctcc ttctcttctt cctcctcctt ttgggctgag gtcaggctct ccgcatcagc
6481 caggttgtcc accgcaatgg ccaagaacac attcagtagg atataatttc cacagatgaa
6541 gaggatgatg aagtaaatac agactaacat ccctggaaaa gaggggccgc cataagccat
6601 gatcccatca tacatcaccg aattccagtc ctccccggtc aggatctgaa acacagtgag
6661 gagagactgc gggaagttat cgaacgtgct cctacgggtc tgcatctcat cgaaattgaa
6721 cttccctcca aagagctgca tccccaggag ggagaagatg atgatgaaga ggaagaggag
6781 cagcagcagg gaggcaatgg agcgcactga gttcagcaag gatgccacaa ggttgctcaa
6841 ggaattccag tacctggtga tcttgaagat cctgagcaac cgcacacatc tcagcacaga
6901 gatgcccagg ggagacatga tcttcgtctc caccaggatg gtctccagga tgcccccaca
6961 cacaatgaaa cagtcaaagc ggttgaagag ggacacaaaa taggcctgaa gacccaggct
7021 gtacatcttc aggagcattt ctgcagtgaa aagggccagg agggctttat tggctgtgtc
7081 ttgcacttct gtgagccagt gaggctggtt gtaatgttcg gaggcaatgg tgagggtgtt
7141 gaggaacacc aggaagatca cgagccagta gaagacgttg gacttaactg ctgcacggca
7201 ttttcttctg cagaatcgat tccacctgcg ccagtagcgg ctgaatttgg atttggagat
7261 ccgatgggca agcctggctc cacagttttc tccctcgatg tcacctccag ccacgttttc
7321 agtgttgaca gactcagtct cacttgtggg catgctcatg tttcgaggct tgtcttcatc
7381 catgccctcg tcctcattct cggggtcaat gtcttctgcc tgggtgatcc agtccaggta
7441 gcctttgaga tcttcttcta gttgctgctt ctctcgaagc ttctggaaat ctcctcgggc
7501 tttggctttc tccctctctt tggaaaactc cccgctcaaa acaccgagaa ccagatttag
7561 aacgaaaaag gatccaaaga tgaccagact gacaaaatac acccagggca actcatagcc
7621 catagcgtct tgcatccagt acagcacgtc tgtccagccc tccatggtga tacactggaa
7681 caccgtcagc atggcgaagg cgaagttgtc gaagttggtg atgccgtgct tgggcccatc
7741 ccacccgggt ttgcacacgg tcccgttctg acactgtcgc ccatggcctg tctccaaagc
7801 acaaggggaa ggatcctctt ctgccggaac atctattatg ccctcctggt tgtagcaggt
7861 cttgtgcatc tttcccatga agagctccag gccgataata gcataaatga tgatgacgaa
7921 gagcacaaga agggcaatgt gcagcagagg caccatggcc ttgatgatgg agttcaggac
7981 cacctggaga cttgggactc cggacactag ccgcagtgga cggagcacgc ggaacgctct
8041 cagtgccttc acgtcgaatc cagctccttt ccctcctaga gcattggccc catcagcttt
8101 ggttgcttgt tctaaaattg cactaaaaag ccctacaacc acgattataa aatccagtaa
8161 attccaacca ttgcggaggt aagcgttggg gtggaagaga agtccgtagg caattacttt
8221 taaaaatgct tccacggtaa aaatgatgag gaagagatac tccactcgtt ccaggttgga
8281 gttggtggcg ttggagtcgt cttccggaaa gggaatatag attgctaagg ccacacaatt
8341 ggcaaaaata gtcagtaaaa taatgatttc aaatggtttc cattcaacaa tgcttatgca
8401 cgccctccgg atagggttct tcagggtcag acacagcaga gcccggggcg gccgtgtggc
8461 ggttgtgccc ccctgcttct tgggtttccc atactgctgc cgcttccgct gtgtggaact
8521 gacggtagag atggttgcgt tgccagcact gcccatgagc ttggcctgcc gggcggcatc
8581 gatggctgcc tgccaggaca gtgctgcccc tggagtaggg atgtgctcgg gagcaagtcc
8641 tgcagctgca ttggcattca tgttggcatg agctgggcgt gggctcccat agttggaacc
8701 ttggtggttt tcctctggaa cgtacatcct cgtgttttca ttgaccattg gccaaaaact
8761 gcttggaaag gagaagccac aaagaggaag aaatgcagca acaagccgaa ttccagcaca
8821 ctggcggccg ttactagtgg atccgagctc ggtaccaagc ttaagtttaa acgctagcca
8881 gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct
8941 ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt
9001 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
9061 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
9121 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
9181 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
9241 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
9301 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
9361 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
9421 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
9481 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa
9541 cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc
9601 ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac
9661 tcagcgacct ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag
9721 cagattgtac tgagagtgca ccatagggga tcgggagatc tcccgatccg tcgacgtcag
9781 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
9841 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
9901 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
9961 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
10021 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
10081 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
10141 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
10201 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
10261 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
10321 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
10381 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
10441 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
10501 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
10561 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
10621 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
10681 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
10741 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
10801 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
10861 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
10921 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
10981 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
11041 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
11101 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
11161 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
11221 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
11281 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
11341 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
11401 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
11461 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
11521 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
11581 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
11641 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
11701 aagcggaag
//
