| 质粒名称: | pAAV-CAG-dCyRFP2s-KDEL |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000735 |
| 产品用途: | AAV, express dCyRFP2s fused to calreticulin |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | dCyRFP2s |
| 物种来源: | Synthetic |
| 密码子优化: | 未优化 |
买家导航
pAAV-CAG-dCyRFP2s-KDEL载体质粒基本信息
pAAV-CAG-dCyRFP2s-KDEL质粒图谱载体图谱和pAAV-CAG-dCyRFP2s-KDEL载体序列质粒序列多克隆位点信息
pAAV-CAG-dCyRFP2s-KDEL质粒载体简介
pAAV-CAG-dCyRFP2s-KDEL质粒序列载体序列
LOCUS pAAV-CAG-dCyRFP2s-KDEL 5505 bp DNA circular SYN 10-APR-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5505)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported May 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5505
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
primer_bind 954..1012
/label=Primer 1
misc_feature 982..987
/label=Kozak sequence
CDS 988..1038
/codon_start=1
/label= signal peptide from calreticulin
/translation="MLLSVPLLLGLLGLAVA"
primer_bind complement(993..1047)
/label=R
primer_bind 1028..1052
/label=0322-PM1103-F
CDS 1048..1749
/codon_start=1
/product="monomeric cyan-excitable red fluorescent protein
(Laviv et al., 2016)"
/label=dCyRFP2s
/translation="MVSKGEELIKESMRSKLYLEGSVNGHQFKCIHEGEGKPYEGKQTA
RIKVVEGGPLPFAFDILATMFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGG
VLTATQDTSLQDGGLIYNVKLRGVNFPAYGPVMQKKTLGWEPSTETMYPADGGLEGRCD
KLLKLVGGGHLHVNFKTTYRSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARY
SNLGGGMDELYK"
regulatory 1048..1051
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind complement(1728..1786)
/label=0322-PM1103-R
misc_feature 1789..2377
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2260..2271)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 2420..2541
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
repeat_region 2703..2832
/label=AAV2 ITR
rep_origin complement(3069..3657)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3828..4688)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4689..4793)
/gene="bla"
/label=AmpR promoter
rep_origin complement(4819..5274)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
repeat_region 5346..5475
/label=AAV2 ITR
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtacccgta agccaccatg ctgctatccg tgccgttgct gctcggcctc
1021 ctcggcctgg ccgtcgccga ccggtcgatg gtgagcaagg gcgaggagct gatcaaggag
1081 tccatgagaa gcaagctgta cctggaaggt agcgtgaacg gccaccagtt caagtgcatc
1141 cacgaagggg agggcaagcc ctacgagggc aagcagaccg cgaggatcaa ggtggtggag
1201 gggggccccc tgccgttcgc attcgacatc ctggccacca tgtttatgta cgggagcaag
1261 gtgttcatca agtaccccgc cgacctcccc gattatttta agcagtcctt ccctgagggc
1321 ttcacatggg agagagtcat ggtgttcgaa gacgggggcg tgctgaccgc cactcaggac
1381 accagcctcc aggacggcgg gctcatctac aacgtcaagc tcagaggggt gaacttccca
1441 gcctacggcc ccgtgatgca gaagaaaaca ctgggctggg agcccagcac cgagaccatg
1501 taccccgctg acggcggcct ggaaggcagg tgcgacaagt tgctgaagct cgtgggcggg
1561 ggccacctgc acgtcaactt caagaccaca tacaggtcca agaaacccgt gaagatgccc
1621 ggcgtccact acgtggaccg cagactggaa agaatcaagg aggccgacaa cgagacctac
1681 gtcgagcagt acgagcacgc tgtggccaga tactccaacc tgggcggagg catggacgag
1741 ctgtacaaga aggacgagct ataagaattc gatatcaagc ttatcgataa tcaacctctg
1801 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
1861 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
1921 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
1981 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
2041 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
2101 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
2161 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
2221 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
2281 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
2341 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acccgggcgg
2401 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
2461 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
2521 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
2581 ggggagatgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
2641 aaggatcttc ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta
2701 caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
2761 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
2821 gcgagcgcgc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
2881 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
2941 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
3001 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
3061 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
3121 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
3181 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
3241 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
3301 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
3361 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
3421 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
3481 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
3541 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
3601 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
3661 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
3721 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
3781 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
3841 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
3901 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
3961 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
4021 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
4081 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
4141 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
4201 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
4261 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
4321 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
4381 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
4441 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
4501 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
4561 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
4621 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
4681 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
4741 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
4801 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
4861 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
4921 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
4981 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
5041 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
5101 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
5161 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
5221 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
5281 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
5341 gccagctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga
5401 cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca
5461 tcactagggg ttccttgtag ttaatgatta acccgccatg ctact
//
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