pAAVLINK-EF1a-HA-ATR-A

LOCUS       pAAVLINK-EF1a-HA-ATR-A                5291 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        146..688
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        175..386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             399..667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    493..498
                     /label=SDS
     CDS             704..730
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             737..2431
                     /codon_start=1
                     /label=ATR
                     /translation="MGEHGLELASMIPALRELGSATPEEYNTVVQKPRQILCQFIDRIL
                     TDVNVVAVELVKKTDSQPTSVMLLDFIQHIMKSSPLMFVNVSGSHEAKGSCIEFSNWII
                     TRLLRIAATPSCHLLHKKICEVICSLLFLFKSKSPAIFGVLTKELLQLFEDLVYLHRRN
                     VMGHAVEWPVVMSRFLSQLDEHMGYLQSAPLQLMSMQNLEFIEVTLLMVLTRIIAIVFF
                     RRQELLLWQIGCVLLEYGSPKIKSLAISFLTELFQLGGLPAQPASTFFSSFLELLKHLV
                     EMDTDQLKLYEEPLSKLIKTLFPFEAEAYRNIEPVYLNMLLEKLCVMFEDGVLMRLKSD
                     LLKAALCHLLQYFLKFVPAGYESALQVRKVYVRNICKALLDVLGIEVDAEYLLGPLYAA
                     LKMESMEIIEEIQCQTQQENLSSNSDGISPKRRRLSSSLNPSKRAPKQTEEIKHVDMNQ
                     KSILWSALKQKAESLQISLEYSGLKNPVIEMLEGIAVVLQLTALCTVHCSHQNMNCRTF
                     KDCQHKSKKKPSVVITWMSLDFYTKVLKSCRSLLESVQKLDLEATIDK"
     misc_feature    1077..1082
                     /label=SDS
     intron          2432..2472
                     /label=Splicing Donor
     misc_feature    2476..2509
                     /label=loxP-JT1571
     repeat_region   2554..2694
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    2554..2683
                     /label=AAV2 ITR
     rep_origin      2769..3224
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3506..3610
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3611..4471
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4642..5230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
      181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
      241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
      421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
      481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
      541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
      601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
      661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
      721 tgactatgct ggatctatgg gggaacatgg cctggagctg gcttccatga tccccgccct
      781 gcgggagctg ggcagtgcca caccagagga atataataca gttgtacaga agccaagaca
      841 aattctgtgt caattcattg accggatact tacagatgta aatgttgttg ctgtagaact
      901 tgtaaagaaa actgactctc agccaacctc cgtgatgttg cttgatttca tccagcatat
      961 catgaaatcc tccccactta tgtttgtaaa tgtgagtgga agccatgagg ccaaaggcag
     1021 ttgtattgaa ttcagtaatt ggatcataac gagacttctg cggattgcag caactccctc
     1081 ctgtcatttg ttacacaaga aaatctgtga agtcatctgt tcattattat ttctttttaa
     1141 aagcaagagt cctgctattt ttggggtcct cacaaaagaa ttattacaac tttttgaaga
     1201 cttggtttac ctccatagaa gaaatgtgat gggtcatgct gtggaatggc cagtggtcat
     1261 gagccgattt ttaagtcaat tagatgaaca catgggatat ttacaatcag ctcctttgca
     1321 gttgatgagt atgcaaaatt tagaatttat tgaagtcact ttattaatgg ttcttactcg
     1381 tattattgca attgtgtttt ttagaaggca agaactctta ctttggcaga taggttgtgt
     1441 tctgctagag tatggtagtc caaaaattaa atccctagca attagctttt taacagaact
     1501 ttttcagctt ggaggactac cagcacaacc agctagcact tttttcagct catttttgga
     1561 attattaaaa caccttgtag aaatggatac tgaccaattg aaactctatg aagagccatt
     1621 atcaaagctg ataaagacac tatttccctt tgaagcagaa gcttatagaa atattgaacc
     1681 tgtctattta aatatgctgc tggaaaaact ctgtgtcatg tttgaagacg gtgtgctcat
     1741 gcggcttaag tctgatttgc taaaagcagc tttgtgccat ttactgcagt atttccttaa
     1801 atttgtgcca gctgggtatg aatctgcttt acaagtcagg aaggtctatg tgagaaatat
     1861 ttgtaaagct cttttggatg tgcttggaat tgaggtagat gcagagtact tgttgggccc
     1921 actttatgca gctttgaaaa tggaaagtat ggaaatcatt gaggagattc aatgccaaac
     1981 tcaacaggaa aacctcagca gtaatagtga tggaatatca cccaaaaggc gtcgtctcag
     2041 ctcgtctcta aacccttcta aaagagcacc aaaacagact gaggaaatta aacatgtgga
     2101 catgaaccaa aagagcatat tatggagtgc actgaaacag aaagctgaat cccttcagat
     2161 ttcccttgaa tacagtggcc taaagaatcc tgttattgag atgttagaag gaattgctgt
     2221 tgtcttacaa ctgactgctc tgtgtactgt tcattgttct catcaaaaca tgaactgccg
     2281 tactttcaag gactgtcaac ataaatccaa gaagaaacct tctgtagtga taacttggat
     2341 gtcattggat ttttacacaa aagtgcttaa gagctgtaga agtttgttag aatctgttca
     2401 gaaactggac ctggaggcaa ccattgataa ggtaagtatc aaggttacaa gacaggttta
     2461 aggagaccaa taagctatta ttcgtatagc atacattata cgaagttatt aagttatgga
     2521 gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
     2581 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     2641 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     2701 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     2761 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     2821 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
     2881 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     2941 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     3001 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3061 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
     3121 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
     3181 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     3241 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     3301 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     3361 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     3421 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     3481 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3541 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3601 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3661 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3721 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3781 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3841 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3901 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3961 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4021 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4081 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4141 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4201 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4261 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4321 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4381 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4441 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4501 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4561 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4621 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4681 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4741 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4801 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4861 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4921 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4981 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5041 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5101 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5161 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5221 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5281 tgctcacatg t
//