pYK407

LOCUS      pYK407               7858 bp DNA     circular SYN 11-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 27, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     881..952
                     /label=oYK317_NLS_NS3a_F
     primer_bind     881..952
                     /label=oYK320_NES_F
     primer_bind     881..951
                     /label=oYK30-NS3a_Head-F
     primer_bind     881..949
                     /label=oYK407_Flag_NS3a_F
     primer_bind     881..900
                     /label=oYK06-ANR-mEGFP-F
     primer_bind     881..900
                     /label=oYK36-AG-Link_s-F
     CDS             904..927
                     /codon_start=1
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     928..969
                     /label=oSCL1082
     primer_bind     928..954
                     /label=oyk03-03-F
     primer_bind     933..954
                     /label=oYK04-ns3a-F
     primer_bind     933..952
                     /label=oYK373_NS3a_F
     CDS             934..1521
                     /codon_start=1
                     /label=NS3a H1
                     /translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
                     EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
                     SRSLTPCTCGSSDLYLVTRHADVIPVRGRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
                     AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
     primer_bind     934..952
                     /label=oYK25-NS3a-F
     primer_bind     951..1009
                     /label=oSCL1083
     primer_bind     990..1048
                     /label=oSCL1084
     primer_bind     1027..1085
                     /label=oSCL1085
     primer_bind     1064..1122
                     /label=oSCL1086
     primer_bind     complement(1092..1111)
                     /label=oSCL1106
     primer_bind     1093..1131
                     /label=oSCL1104
     primer_bind     complement(1328..1386)
                     /label=oSCL1105
     primer_bind     complement(1368..1439)
                     /label=oSCL1087
     primer_bind     1370..1391
                     /label=oSCL1107
     primer_bind     1421..1439
                     /label=oSCL1088
     primer_bind     complement(1503..1527)
                     /label=oSCL1089
     primer_bind     complement(1506..1538)
                     /label=oYK30-NS3a_head-R
     primer_bind     complement(1509..1550)
                     /label=oYK66-NS3a-R
     misc_feature    1528..1538
                     /label=linker
     CDS             1531..1605
                     /codon_start=1
                     /label=5xGS Linker
                     /translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
     primer_bind     1531..1551
                     
     primer_bind     1531..1549
                     /label=Primer 1
     primer_bind     complement(1580..1608)
                     /label=olyl212
     primer_bind     complement(1580..1605)
                     /label=olyl234
     primer_bind     complement(1589..1625)
                     /label=oYK45-46 ANR1-R
     primer_bind     1605..1624
                     /label=oYK36-AG-Link_s-F
     primer_bind     1606..1625
                     /label=oYK41-44 AG-LplA-F
     primer_bind     1606..1625
                     /label=oYK45-46 AG-LplA-F
     misc_feature    1609..2283
                     /label=AG
     primer_bind     2057..2067
                     /label=oSCL1104
     misc_feature    2284..2334
                     /label=Short_Linker
     primer_bind     complement(2310..2354)
                     /label=oYK36-AG-Link_s-R
     primer_bind     complement(2310..2334)
                     /label=oYK33-35-PB1-AG-R
     misc_feature    2335..3356
                     /label=LPLA
                     /note="LplA"
     primer_bind     2335..2353
                     /label=oYK36-37-LplA-F
     primer_bind     2611..2633
                     /label=oYK08_lpl91-F
     primer_bind     2673..2689
                     /label=oYK09_lpl112-F
     primer_bind     2719..2734
                     /label=oYK10_lpl127-F
     primer_bind     2764..2782
                     /label=oYK11_lpl142-F
     primer_bind     2800..2818
                     /label=oYK12_lpl154-F
     primer_bind     2863..2885
                     /label=oYK13_lpl175-F
     primer_bind     2911..2929
                     /label=oYK14_lpl191-F
     primer_bind     2974..2989
                     /label=oYK15-lpl212-F
     primer_bind     3000..3019
                     /label=oYK16_lpl221-F
     primer_bind     3058..3079
                     /label=oYK17_lpl240-F
     primer_bind     3082..3100
                     /label=oYK18_lpl248-F
     primer_bind     3118..3132
                     /label=oYK19_lpl260-F
                     /note="Maybe mismatch"
     primer_bind     3154..3172
                     /label=oYK20_lpl272-F
     primer_bind     3185..3201
                     /label=oYK21_lpl283-F
     primer_bind     3232..3249
                     /label=oYK22_lpl298-F
                     /note="DNA may less than 100 bp"
     primer_bind     complement(3332..3351)
                     
     primer_bind     complement(3334..3351)
                     /label=oYK404_LplA_R
     primer_bind     complement(3336..3351)
                     /label=oYK228_lplA_R
     primer_bind     complement(3336..3351)
                     /label=oYK341_lplA_R
     primer_bind     complement(3336..3351)
                     /label=oYK372_LplA_R
     primer_bind     complement(3341..3361)
                     /label=oYK01-1-R
     primer_bind     complement(3351..3375)
                     /label=oYK08-22-LplApart-R
     primer_bind     complement(3354..3375)
                     /label=oYK36-37-LplA-R
     primer_bind     complement(3356..3378)
                     /label=oYK57-MCP-mCherry-R
     primer_bind     complement(3380..3399)
                     /label=oSCL1089
     terminator      3452..3679
                     /label=bGH PA
     misc_feature    3725..4153
                     /label=f1 origin
     misc_feature    4158..4501
                     /label=SV40 early promoter and origin
     misc_feature    4347..4424
                     /label=SV40 ORI
     CDS             4566..5357
                     /label=Kan/neoR
     misc_feature    5531..5661
                     /label=SV40 early polyadenylation signal
     primer_bind     complement(5697..5717)
                     /label=M13-rev
     misc_binding    complement(5723..5745)
                     /label=LacO
     promoter        complement(5750..5779)
                     /label=lac
     misc_feature    complement(6044..6717)
                     /label=pUC origin
     CDS             complement(6865..7524)
                     /label=AmpR
     primer_bind     7181..7192
                     /label=oyk03-03-F
     primer_bind     7181..7192
                     /label=oYK04-ns3a-F
     promoter        complement(7764..7792)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 atggattaca aggatgacga cgataaggga tccaagaaga agggcagcgt ggtgatcgtg
      961 ggcagaatca acctgagcgg cgacaccgcc tacagccagc agaccagagg cctggagggc
     1021 tgccaggaga ccagccagac cggcagagac aagaaccagg tggagggcga ggtgcaggtg
     1081 gtgagcaccg ccacccagag cttcctggcc accagcatca acggcgtgct gtggaccgtg
     1141 taccacggcg ccggcaccag aaccatcgcc agccccaagg gccccgtgac ccagatgtac
     1201 acgaacgtgg acaaggactt agtgggctgg caggcccccc aaggcagcag aagcctgacc
     1261 ccctgcacct gcggcagcag cgacctgtac ctggtgacca gacacgccga cgtgatcccc
     1321 gtgagaggaa gaggcgacag cagaggcagc ctgctgagcc ccagacccat cagctatctg
     1381 aaaggctcaa gcggcggccc cctgttgtgt cccgccggcc atgccgtggg catcttcaga
     1441 gccgccgtga gcaccagagg cgtggccaag gccgtggact tcatccccgt ggagagcctg
     1501 gagaccacca tgagaagccc cgctagcagc ggaagtggaa ctgggtctgg gactgggtcc
     1561 ggcaccggga ctacttcagg caccggcact gggggctcca ctggcatggt gagcgtgatc
     1621 aagcccgaga tgaagatcaa gctgtgcatg aggggcaccg tgaacggcca caacttcgtg
     1681 atcgagggcg agggcaaggg caacccctac gagggcaccc agatcctgga cctgaacgtg
     1741 accgagggcg cccccctgcc cttcgcctac gacatcctga ccaccgtgtt ccagtacggc
     1801 aacagggcct tcaccaagta ccccgccgac atccaggact acttcaagca gaccttcccc
     1861 gagggctacc actgggagag gagcatgacc tacgaggacc agggcatctg caccgccacc
     1921 agcaacatca gcatgagggg cgactgcttc ttctacgaca tcaggttcga cggcgtgaac
     1981 ttccccccca acggccccgt gatgcagaag aagaccctga agtgggagcc cagcaccgag
     2041 aagatgtacg tgagggacgg cgtgctgaag ggcgacgtga acatggccct gctgctggag
     2101 ggcggcggcc actacaggtg cgacttcaag accacctaca aggccaagaa ggacgtgagg
     2161 ctgcccgact accacttcgt ggaccacagg atcgagatcc tgaagcacga caaggactac
     2221 aacaaggtga agctgtacga gaacgccgtg gccaggtaca gcatgctgcc cagccaggcc
     2281 aagggtaccg gaactgcagc agagaattcg ggaaactcga gaacaaagct tgaaatgggc
     2341 tccacattac gcctgctcat ctctgactct tacgacccgt ggtttaacct ggcggtggaa
     2401 gagtgtattt ttcgccaaat gcccgccacg cagcgcgttc tgtttctctg gcgcaatgcc
     2461 gacacggtag taattggtcg cgcgcagaac ccgtggaaag agtgtaatac ccggcggatg
     2521 gaagaagata acgtccgcct ggcgcggcgc agtagcggtg gcggcgcggt gttccacgat
     2581 ctcggcaata cctgctttac ctttatggct ggcaagccgg agtacgataa aactatctcc
     2641 acgtcgattg tgctcaatgc gctgaacgcg ctcggcgtca gcgccgaagc gtccggacgt
     2701 aacgatctgg tggtgaaaac cgtcgaaggc gaccgcaaag tctcaggctc ggcctatcgc
     2761 gaaaccaaag atcgcggctt ccaccacggc accttgctac tcaatgccga cctcagccgc
     2821 ctggcaaact atctcaatcc ggataaaaag aaactggcgg cgaaaggcat tacgtcggta
     2881 cgttcccgcg tgaccaacct caccgagctg ttgccgggga tcacccatga gcaggtttgc
     2941 gaggccataa ccgaggcctt tttcgcccat tatggcgagc gcgtggaagc ggaaatcatc
     3001 tccccgaaca aaacgccaga cttgccaaac ttcgccgaaa cctttgcccg ccagagtagc
     3061 tgggaatgga acttcggtca ggctccggca ttctcgcatc tgctggatga acgctttacc
     3121 tggggcggcg tggaactgca tttcgacgtt gaaaaaggcc atatcacccg cgcccaggtg
     3181 tttaccgaca gcctcaaccc cgcgccgctg gaagccctcg ccggacgact gcaaggctgc
     3241 ctgtaccgcg cagatatgct gcaacaggag tgcgaagcgc tgttggttga cttcccggaa
     3301 caggaaaaag agctacggga gttatcggca tggatggcgg gggctgtaag gtaggggatc
     3361 cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc gctcgagtct
     3421 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     3481 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3541 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3601 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3661 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     3721 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3781 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3841 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3901 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     3961 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4021 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4081 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4141 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     4201 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     4261 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4321 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4381 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     4441 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     4501 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     4561 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     4621 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4681 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4741 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4801 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4861 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4921 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4981 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     5041 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     5101 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     5161 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     5221 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     5281 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     5341 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     5401 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     5461 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     5521 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5581 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5641 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     5701 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5761 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5821 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5881 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5941 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6001 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6061 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6121 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6181 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6241 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6301 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6361 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6421 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6481 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6541 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6601 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6661 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6721 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6781 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6841 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6901 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6961 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7021 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7081 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7141 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     7201 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7261 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7321 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7381 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7441 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     7501 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7561 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7621 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7681 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7741 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7801 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//