phKDR

LOCUS       phKDR        9488 bp DNA     circular SYN 11-FEB-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 28, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     198..258
                     /label=oQX494_pLL3.7-U6-BsmBI-CMVe-recF1
     primer_bind     234..274
                     /label=oQX488_pLL3.7-CMVe-recF1
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     662..681
                     /label=oQX329_dCMVp-F
     primer_bind     664..687
                     /label=oQX17_pQX3/4/5-dF
     primer_bind     807..830
                     /label=oYW200
     primer_bind     complement(823..847)
                     /label=oQX489_pLL3.7-MCS-CMV-recR1
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     916..958
                     /label=oQX1146_BamHI-hKDR-recF
     CDS             941..5011
                     /label=hKDR
     primer_bind     complement(1866..1884)
                     /label=oQX1171_hKDR-dR4
     primer_bind     complement(2622..2639)
                     /label=oQX1170_hKDR-dR3
     primer_bind     complement(3422..3440)
                     /label=oQX1169_hKDR-dR2
     primer_bind     complement(4240..4263)
                     /label=oQX1168_hKDR-dR1
     primer_bind     complement(4990..5030)
                     /label=oQX1147_EcoRI-hKDR-recR
     primer_bind     complement(5061..5081)
                     /label=oQX696_3.1-ApaI-R
     CDS             5085..5312
                     /codon_start=1
                     /label=bGH polyA
                     /translation="RLCLLVASHLLFAPPPCLP*PWKVPLPLSFPNKMRKLHRIV*VGV
                     ILFWGVGWGRTARGRIGKTIAGMLGMRWALW"
     primer_bind     complement(5092..5109)
                     /label=oYW190
     primer_bind     complement(5125..5145)
                     /label=oQX18_pQX3/4/5-dR
     primer_bind     complement(5154..5172)
                     /label=oYW57
     rep_origin      5358..5786
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5800..6129
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5980..6115
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6032..6081
                     /codon_start=1
                     /label=P_SV40 TSS-region
                     /translation="*FFLFMQRPRPPLPLS"
     CDS             6196..6990
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     6250..6269
                     
     primer_bind     complement(6250..6269)
                     
     polyA_signal    7164..7285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7334..7350)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7358..7374)
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7382..7412)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7427..7448
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7736..8321)
                     /direction=LEFT
                     /label=high-copy number ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8032..8056)
                     /label=oQX766_ori-F
     primer_bind     8036..8057
                     /label=oQX767_ori-R
     CDS             complement(8492..9352)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9111..9154)
                     /label=oQX6_AmpR-R
     primer_bind     9126..9155
                     /label=oQX337_AmpR-recF
     primer_bind     complement(9126..9152)
                     /label=oQX768_AmpR-recR
     primer_bind     9133..9154
                     /label=oQX5_BsmBI-recom-F
     primer_bind     9323..9342
                     /label=oYW24
     promoter        complement(9353..9457)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgcagagca aggtgctgct
      961 ggccgtcgcc ctgtggctct gcgtggagac ccgggccgcc tctgtgggtt tgcctagtgt
     1021 ttctcttgat ctgcccaggc tcagcataca aaaagacata cttacaatta aggctaatac
     1081 aactcttcaa attacttgca ggggacagag ggacttggac tggctttggc ccaataatca
     1141 gagtggcagt gagcaaaggg tggaggtgac tgagtgcagc gatggcctct tctgtaagac
     1201 actcacaatt ccaaaagtga tcggaaatga cactggagcc tacaagtgct tctaccggga
     1261 aactgacttg gcctcggtca tttatgtcta tgttcaagat tacagatctc catttattgc
     1321 ttctgttagt gaccaacatg gagtcgtgta cattactgag aacaaaaaca aaactgtggt
     1381 gattccatgt ctcgggtcca tttcaaatct caacgtgtca ctttgtgcaa gatacccaga
     1441 aaagagattt gttcctgatg gtaacagaat ttcctgggac agcaagaagg gctttactat
     1501 tcccagctac atgatcagct atgctggcat ggtcttctgt gaagcaaaaa ttaatgatga
     1561 aagttaccag tctattatgt acatagttgt cgttgtaggg tataggattt atgatgtggt
     1621 tctgagtccg tctcatggaa ttgaactatc tgttggagaa aagcttgtct taaattgtac
     1681 agcaagaact gaactaaatg tggggattga cttcaactgg gaataccctt cttcgaagca
     1741 tcagcataag aaacttgtaa accgagacct aaaaacccag tctgggagtg agatgaagaa
     1801 atttttgagc accttaacta tagatggtgt aacccggagt gaccaaggat tgtacacctg
     1861 tgcagcatcc agtgggctga tgaccaagaa gaacagcaca tttgtcaggg tccatgaaaa
     1921 accttttgtt gcttttggaa gtggcatgga atctctggtg gaagccacgg tgggggagcg
     1981 tgtcagaatc cctgcgaagt accttggtta cccaccccca gaaataaaat ggtataaaaa
     2041 tggaataccc cttgagtcca atcacacaat taaagcgggg catgtactga cgattatgga
     2101 agtgagtgaa agagacacag gaaattacac tgtcatcctt accaatccca tttcaaagga
     2161 gaagcagagc catgtggtct ctctggttgt gtatgtccca ccccagattg gtgagaaatc
     2221 tctaatctct cctgtggatt cctaccagta cggcaccact caaacgctga catgtacggt
     2281 ctatgccatt cctcccccgc atcacatcca ctggtattgg cagttggagg aagagtgcgc
     2341 caacgagccc agccaagctg tctcagtgac aaacccatac ccttgtgaag aatggagaag
     2401 tgtggaggac ttccagggag gaaataaaat tgaagttaat aaaaatcaat ttgctctaat
     2461 tgaaggaaaa aacaaaactg taagtaccct tgttatccaa gcggcaaatg tgtcagcttt
     2521 gtacaaatgt gaagcggtca acaaagtcgg gagaggagag agggtgatct ccttccacgt
     2581 gaccaggggt cctgaaatta ctttgcaacc tgacatgcag cccactgagc aggagagcgt
     2641 gtctttgtgg tgcactgcag acagatctac gtttgagaac ctcacatggt acaagcttgg
     2701 cccacagcct ctgccaatcc atgtgggaga gttgcccaca cctgtttgca agaacttgga
     2761 tactctttgg aaattgaatg ccaccatgtt ctctaatagc acaaatgaca ttttgatcat
     2821 ggagcttaag aatgcatcct tgcaggacca aggagactat gtctgccttg ctcaagacag
     2881 gaagaccaag aaaagacatt gcgtggtcag gcagctcaca gtcctagagc gtgtggcacc
     2941 cacgatcaca ggaaacctgg agaatcagac gacaagtatt ggggaaagca tcgaagtctc
     3001 atgcacggca tctgggaatc cccctccaca gatcatgtgg tttaaagata atgagaccct
     3061 tgtagaagac tcaggcattg tattgaagga tgggaaccgg aacctcacta tccgcagagt
     3121 gaggaaggag gacgaaggcc tctacacctg ccaggcatgc agtgttcttg gctgtgcaaa
     3181 agtggaggca tttttcataa tagaaggtgc ccaggaaaag acgaacttgg aaatcattat
     3241 tctagtaggc acggcggtga ttgccatgtt cttctggcta cttcttgtca tcatcctacg
     3301 gaccgttaag cgggccaatg gaggggaact gaagacaggc tacttgtcca tcgtcatgga
     3361 tccagatgaa ctcccattgg atgaacattg tgaacgactg ccttatgatg ccagcaaatg
     3421 ggaattcccc agagaccggc tgaagctagg taagcctctt ggccgtggtg cctttggcca
     3481 agtgattgaa gcagatgcct ttggaattga caagacagca acttgcagga cagtagcagt
     3541 caaaatgttg aaagaaggag caacacacag tgagcatcga gctctcatgt ctgaactcaa
     3601 gatcctcatt catattggtc accatctcaa tgtggtcaac cttctaggtg cctgtaccaa
     3661 gccaggaggg ccactcatgg tgattgtgga attctgcaaa tttggaaacc tgtccactta
     3721 cctgaggagc aagagaaatg aatttgtccc ctacaagacc aaaggggcac gattccgtca
     3781 agggaaagac tacgttggag caatccctgt ggatctgaaa cggcgcttgg acagcatcac
     3841 cagtagccag agctcagcca gctctggatt tgtggaggag aagtccctca gtgatgtaga
     3901 agaagaggaa gctcctgaag atctgtataa ggacttcctg accttggagc atctcatctg
     3961 ttacagcttc caagtggcta agggcatgga gttcttggca tcgcgaaagt gtatccacag
     4021 ggacctggcg gcacgaaata tcctcttatc ggagaagaac gtggttaaaa tctgtgactt
     4081 tggcttggcc cgggatattt ataaagatcc agattatgtc agaaaaggag atgctcgcct
     4141 ccctttgaaa tggatggccc cagaaacaat ttttgacaga gtgtacacaa tccagagtga
     4201 cgtctggtct tttggtgttt tgctgtggga aatattttcc ttaggtgctt ctccatatcc
     4261 tggggtaaag attgatgaag aattttgtag gcgattgaaa gaaggaacta gaatgagggc
     4321 ccctgattat actacaccag aaatgtacca gaccatgctg gactgctggc acggggagcc
     4381 cagtcagaga cccacgtttt cagagttggt ggaacatttg ggaaatctct tgcaagctaa
     4441 tgctcagcag gatggcaaag actacattgt tcttccgata tcagagactt tgagcatgga
     4501 agaggattct ggactctctc tgcctacctc acctgtttcc tgtatggagg aggaggaagt
     4561 atgtgacccc aaattccatt atgacaacac agcaggaatc agtcagtatc tgcagaacag
     4621 taagcgaaag agccggcctg tgagtgtaaa aacatttgaa gatatcccgt tagaagaacc
     4681 agaagtaaaa gtaatcccag atgacaacca gacggacagt ggtatggttc ttgcctcaga
     4741 agagctgaaa actttggaag acagaaccaa attatctcca tcttttggtg gaatggtgcc
     4801 cagcaaaagc agggagtctg tggcatctga aggctcaaac cagacaagcg gctaccagtc
     4861 cggatatcac tccgatgaca cagacaccac cgtgtactcc agtgaggaag cagaactttt
     4921 aaagctgata gagattggag tgcaaaccgg tagcacagcc cagattctcc agcctgactc
     4981 ggggaccaca ctgagctctc ctcctgttta ggaattctgc agatatccag cacagtggcg
     5041 gccgctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     5101 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     5161 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     5221 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     5281 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     5341 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5401 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5461 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5521 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5581 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5641 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     5701 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     5761 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     5821 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5881 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5941 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     6001 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6061 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     6121 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
     6181 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     6241 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     6301 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     6361 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     6421 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     6481 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     6541 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     6601 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     6661 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     6721 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     6781 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     6841 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     6901 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     6961 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     7021 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     7081 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     7141 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     7201 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7261 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     7321 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7381 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7441 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7501 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7561 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7621 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7681 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7741 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7801 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7861 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7921 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7981 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8041 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8101 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8161 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8221 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg
     8281 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8341 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8401 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8461 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8521 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8581 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8641 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8701 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8761 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8821 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8881 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8941 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9001 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9061 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9121 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9181 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9241 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9301 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9361 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9421 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9481 ctgacgtc
//