pCMV-hA3A-BE3-D131Y

LOCUS       pCMV-hA3A-BE3-D131Y                8442 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses hA3A-BE3-D131Y in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-hA3A-BE3-D131Y
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Wang X, Li J, Wang Y, Yang V, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol.,  2018
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        133..512
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        513..716
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     667..687
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     758..777
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        758..776
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      784..793
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             790..1386
                     /codon_start=1
                     /gene="human APOBEC3A"
                     /gene_synonym="A3A"
                     /product="human cytidine deaminase (C to U editing enzyme) 
                     that efficiently edits methylated cytosines (Wang et al., 
                     2018)"
                     /label=APOBEC3A
                     /note="also known as hA3A"
                     /translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS
                     VKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSW
                     GCAGEVRAFLQENTHVRLRIFAARIYYYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD
                     TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     CDS             1435..5535
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5548..5796
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5809..5829
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5838..5855
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5878..5895)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5884..6108
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6179..6195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6179..6195)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6192..6214)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6203..6219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6227..6257)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6272..6293
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6410..6427)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6581..7169)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6661..6680)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7340..8200)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7963..7982
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8201..8305)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8384..8403)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
       61 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
      121 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      181 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      241 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      301 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      361 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      421 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      481 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      541 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      601 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      661 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
      721 tagtgaaccg tcagatccgc tagagatccg cggccgctaa tacgactcac tatagggaga
      781 gccgccacca tggaagccag cccagcatcc gggcccagac acttgatgga tccacacata
      841 ttcacttcca actttaacaa tggcattgga aggcataaga cctacctgtg ctacgaagtg
      901 gagcgcctgg acaatggcac ctcggtcaag atggaccagc acaggggctt tctacacaac
      961 caggctaaga atcttctctg tggcttttac ggccgccatg cggagctgcg cttcttggac
     1021 ctggttcctt ctttgcagtt ggacccggcc cagatctaca gggtcacttg gttcatctcc
     1081 tggagcccct gcttctcctg gggctgtgcc ggggaagtgc gtgcgttcct tcaggagaac
     1141 acacacgtga gactgcgtat cttcgctgcc cgcatctatt attacgaccc cctatataag
     1201 gaggcactgc aaatgctgcg ggatgctggg gcccaagtct ccatcatgac ctacgatgaa
     1261 tttaagcact gctgggacac ctttgtggac caccagggat gtcccttcca gccctgggat
     1321 ggactagatg agcacagcca agccctgagt gggaggctgc gggccattct ccagaatcag
     1381 ggaaacagcg gcagcgagac tcccgggacc tcagagtccg ccacacccga aagtgataaa
     1441 aagtattcta ttggtttagc catcggcact aattccgttg gatgggctgt cataaccgat
     1501 gaatacaaag taccttcaaa gaaatttaag gtgttgggga acacagaccg tcattcgatt
     1561 aaaaagaatc ttatcggtgc cctcctattc gatagtggcg aaacggcaga ggcgactcgc
     1621 ctgaaacgaa ccgctcggag aaggtataca cgtcgcaaga accgaatatg ttacttacaa
     1681 gaaattttta gcaatgagat ggccaaagtt gacgattctt tctttcaccg tttggaagag
     1741 tccttccttg tcgaagagga caagaaacat gaacggcacc ccatctttgg aaacatagta
     1801 gatgaggtgg catatcatga aaagtaccca acgatttatc acctcagaaa aaagctagtt
     1861 gactcaactg ataaagcgga cctgaggtta atctacttgg ctcttgccca tatgataaag
     1921 ttccgtgggc actttctcat tgagggtgat ctaaatccgg acaactcgga tgtcgacaaa
     1981 ctgttcatcc agttagtaca aacctataat cagttgtttg aagagaaccc tataaatgca
     2041 agtggcgtgg atgcgaaggc tattcttagc gcccgcctct ctaaatcccg acggctagaa
     2101 aacctgatcg cacaattacc cggagagaag aaaaatgggt tgttcggtaa ccttatagcg
     2161 ctctcactag gcctgacacc aaattttaag tcgaacttcg acttagctga agatgccaaa
     2221 ttgcagctta gtaaggacac gtacgatgac gatctcgaca atctactggc acaaattgga
     2281 gatcagtatg cggacttatt tttggctgcc aaaaacctta gcgatgcaat cctcctatct
     2341 gacatactga gagttaatac tgagattacc aaggcgccgt tatccgcttc aatgatcaaa
     2401 aggtacgatg aacatcacca agacttgaca cttctcaagg ccctagtccg tcagcaactg
     2461 cctgagaaat ataaggaaat attctttgat cagtcgaaaa acgggtacgc aggttatatt
     2521 gacggcggag cgagtcaaga ggaattctac aagtttatca aacccatatt agagaagatg
     2581 gatgggacgg aagagttgct tgtaaaactc aatcgcgaag atctactgcg aaagcagcgg
     2641 actttcgaca acggtagcat tccacatcaa atccacttag gcgaattgca tgctatactt
     2701 agaaggcagg aggattttta tccgttcctc aaagacaatc gtgaaaagat tgagaaaatc
     2761 ctaacctttc gcatacctta ctatgtggga cccctggccc gagggaactc tcggttcgca
     2821 tggatgacaa gaaagtccga agaaacgatt actccatgga attttgagga agttgtcgat
     2881 aaaggtgcgt cagctcaatc gttcatcgag aggatgacca actttgacaa gaatttaccg
     2941 aacgaaaaag tattgcctaa gcacagttta ctttacgagt atttcacagt gtacaatgaa
     3001 ctcacgaaag ttaagtatgt cactgagggc atgcgtaaac ccgcctttct aagcggagaa
     3061 cagaagaaag caatagtaga tctgttattc aagaccaacc gcaaagtgac agttaagcaa
     3121 ttgaaagagg actactttaa gaaaattgaa tgcttcgatt ctgtcgagat ctccggggta
     3181 gaagatcgat ttaatgcgtc acttggtacg tatcatgacc tcctaaagat aattaaagat
     3241 aaggacttcc tggataacga agagaatgaa gatatcttag aagatatagt gttgactctt
     3301 accctctttg aagatcggga aatgattgag gaaagactaa aaacatacgc tcacctgttc
     3361 gacgataagg ttatgaaaca gttaaagagg cgtcgctata cgggctgggg acgattgtcg
     3421 cggaaactta tcaacgggat aagagacaag caaagtggta aaactattct cgattttcta
     3481 aagagcgacg gcttcgccaa taggaacttt atgcagctga tccatgatga ctctttaacc
     3541 ttcaaagagg atatacaaaa ggcacaggtt tccggacaag gggactcatt gcacgaacat
     3601 attgcgaatc ttgctggttc gccagccatc aaaaagggca tactccagac agtcaaagta
     3661 gtggatgagc tagttaaggt catgggacgt cacaaaccgg aaaacattgt aatcgagatg
     3721 gcacgcgaaa atcaaacgac tcagaagggg caaaaaaaca gtcgagagcg gatgaagaga
     3781 atagaagagg gtattaaaga actgggcagc cagatcttaa aggagcatcc tgtggaaaat
     3841 acccaattgc agaacgagaa actttacctc tattacctac aaaatggaag ggacatgtat
     3901 gttgatcagg aactggacat aaaccgttta tctgattacg acgtcgatca cattgtaccc
     3961 caatcctttt tgaaggacga ttcaatcgac aataaagtgc ttacacgctc ggataagaac
     4021 cgagggaaaa gtgacaatgt tccaagcgag gaagtcgtaa agaaaatgaa gaactattgg
     4081 cggcagctcc taaatgcgaa actgataacg caaagaaagt tcgataactt aactaaagct
     4141 gagaggggtg gcttgtctga acttgacaag gccggattta ttaaacgtca gctcgtggaa
     4201 acccgccaaa tcacaaagca tgttgcacag atactagatt cccgaatgaa tacgaaatac
     4261 gacgagaacg ataagctgat tcgggaagtc aaagtaatca ctttaaagtc aaaattggtg
     4321 tcggacttca gaaaggattt tcaattctat aaagttaggg agataaataa ctaccaccat
     4381 gcgcacgacg cttatcttaa tgccgtcgta gggaccgcac tcattaagaa atacccgaag
     4441 ctagaaagtg agtttgtgta tggtgattac aaagtttatg acgtccgtaa gatgatcgcg
     4501 aaaagcgaac aggagatagg caaggctaca gccaaatact tcttttattc taacattatg
     4561 aatttcttta agacggaaat cactctggca aacggagaga tacgcaaacg acctttaatt
     4621 gaaaccaatg gggagacagg tgaaatcgta tgggataagg gccgggactt cgcgacggtg
     4681 agaaaagttt tgtccatgcc ccaagtcaac atagtaaaga aaactgaggt gcagaccgga
     4741 gggttttcaa aggaatcgat tcttccaaaa aggaatagtg ataagctcat cgctcgtaaa
     4801 aaggactggg acccgaaaaa gtacggtggc ttcgatagcc ctacagttgc ctattctgtc
     4861 ctagtagtgg caaaagttga gaagggaaaa tccaagaaac tgaagtcagt caaagaatta
     4921 ttggggataa cgattatgga gcgctcgtct tttgaaaaga accccatcga cttccttgag
     4981 gcgaaaggtt acaaggaagt aaaaaaggat ctcataatta aactaccaaa gtatagtctg
     5041 tttgagttag aaaatggccg aaaacggatg ttggctagcg ccggagagct tcaaaagggg
     5101 aacgaactcg cactaccgtc taaatacgtg aatttcctgt atttagcgtc ccattacgag
     5161 aagttgaaag gttcacctga agataacgaa cagaagcaac tttttgttga gcagcacaaa
     5221 cattatctcg acgaaatcat agagcaaatt tcggaattca gtaagagagt catcctagct
     5281 gatgccaatc tggacaaagt attaagcgca tacaacaagc acagggataa acccatacgt
     5341 gagcaggcgg aaaatattat ccatttgttt actcttacca acctcggcgc tccagccgca
     5401 ttcaagtatt ttgacacaac gatagatcgc aaacgataca cttctaccaa ggaggtgcta
     5461 gacgcgacac tgattcacca atccatcacg ggattatatg aaactcggat agatttgtca
     5521 cagcttgggg gtgactctgg tggttctact aatctgtcag atattattga aaaggagacc
     5581 ggtaagcaac tggttatcca ggaatccatc ctcatgctcc cagaggaggt ggaagaagtc
     5641 attgggaaca agccggaaag cgatatactc gtgcacaccg cctacgacga gagcaccgac
     5701 gagaatgtca tgcttctgac tagcgacgcc cctgaataca agccttgggc tctggtcata
     5761 caggatagca acggtgagaa caagattaag atgctctctg gtggttctcc caagaagaag
     5821 aggaaagtct aaccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct
     5881 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     5941 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     6001 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     6061 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     6121 aaagaaccag ctggggctcg ataccgtcga cctctagcta gagcttggcg taatcatggt
     6181 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6241 gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6301 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6361 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6421 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6481 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6541 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6601 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6661 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6721 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6781 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6841 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6901 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6961 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7021 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7081 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7141 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7201 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7261 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7321 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7381 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7441 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7501 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7561 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7621 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7681 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7741 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7801 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7861 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7921 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7981 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8041 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8101 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8161 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8221 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8281 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     8341 gatcgggaga tcgatctccc gatcccctag ggtcgactct cagtacaatc tgctctgatg
     8401 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt gg
//