pMal-c5x-A0A812Q573

LOCUS      pMal-c5x-A0A812Q573           8129 bp DNA     circular SYN 25-JUL-2024
DEFINITION  5_Syp-A0A812Q573_pMAL-c5x.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..80
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             81..1163
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        1406..1434
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1442..1458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1528..2628
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     CDS             2677..2688
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      5215..5301
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5393..5420
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5448..5538
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5539..6399
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6487..7075
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7445..7636)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatttcggta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag
     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
     1501 gcaattgacc aacaaggacc atagattatg aaaatcgaag aaggtaaact ggtaatctgg
     1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
     2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
     2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     2461 ttggtgaaag atccgcgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg
     2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
     2641 aacaacaaca acaataacaa taacaacaac ctcgggatcg agggaaggat ttcacatatg
     2701 gaatcaaggt gcgcatgttg tgctgtaact gacgcagaaa ccgacgcggc gaccaccggt
     2761 gcgaaaaccg aaaatctgga gatgccgtcc tttgagccga gggatgtggg tagtgcggaa
     2821 cagctgatgc tgtctcgcat gccggcgggt cacatgatta cgactcatac ctcgctggcg
     2881 cttctggagg gtcaggttga cgtcgaacgc ctgaaaaagg cactgagctg ggcaattctg
     2941 cggcacccga tgatgcgtgc gaccgcacgt gaaccaaccg tgctcggtaa agataccggt
     3001 ccgttcttcc acggcggcca agatggccgt tggcgttggg aaccgaccgg tctgggtaaa
     3061 gaagagctaa cggagcgcgg cttgatggtt gaggatgtca gcggcgactt cgactcaaaa
     3121 tgtaaagaac atttcgagca aagcctggac aagaccacct ttgacttcga gaacggccct
     3181 ttatggaaat tgcgtttgct gcgtcaaccg ggcttccgcg gtccgagagg cggccgttct
     3241 gctttattgt tcagctttgt tcactccatt gatgatcaga agagcgcgaa tattctgctt
     3301 cacgatttgc tgagccacat ggaagccgcg gaaaagggtg accccatcga ggatccgaag
     3361 ccggcagccc tgccggcttc gctggaagac gtatttctac aggatgaact ggacgtgcag
     3421 aaactggcgg gttatgccct gagccaggca tctgccggag cggttccgtt tatgaaagtg
     3481 ccgagcgcgt tacgttcagc agagcgtaat gtacgcaaga acttcggttt gaatccggcg
     3541 cagccggtgg cacaagagcg caacatgacg gttccgaccg cggcggctga aggtaacgcg
     3601 aaactgctga tggcggaggc tgtgagctct gaaagcgact tctggagcga gtctcgtaaa
     3661 aacgttgtta ccttccgttc gcttccagca caacatctgt ctcaactgcg ccagctgtgt
     3721 cgtgagaaca atgtgaccat gagcatggcc gttgcggccg ctgcgctgct ggcgaccagc
     3781 gacatcagca acgacgacat ggatttcgcc tacgaagcat atcgtatgct cctcggcgtt
     3841 gatatgcgtc gttttgcccc aggtggggac tggacccgag gcacgatggc gtacgcttcg
     3901 ggagcattgg actttaccct gcgcttgctg ccgaagtctg gtcaagcata cgcggccgag
     3961 cacgaaaatg catcaattag aagccgcatt ggtggtgtgc cgttctggga tctggcgcgc
     4021 gctgcggacc tggcagttcg tgaatgggcg gagaaagggt acgcagcgga gtccactcgt
     4081 ctgttcgaca tcggtatccg tctgttgcgc atggacaaca tcatccgtga gactgcgaac
     4141 gatccgtcca ccctcggtcg tgcttatagc gttaccgttt ccaacgctgg catttatagc
     4201 caaggtccgg acaacggtag ctatggtagc ctgcgtttgg acaagatctt tttcggcatc
     4261 agcaccgcga ttagcggtag cctgatgagc gcgtcttgtc tgaccgtgaa cggcgacttg
     4321 ttgattacgg cccattccgc agcgccggtg gtgaatcgtt ccgacatgga gatctttgcc
     4381 gattccatga tccgttcctt gagcattgca gctatggaaa ccgcgccgaa ggctagcatg
     4441 ggtactcctc cggtggagaa cccgatcgac ccacgtggcg gtctcccttg gtactacctg
     4501 ttggagaccc cgaaaggcac actgcagtgt ccgaagtatg aggacgttag atccccgacc
     4561 ctgccggcgt ttaacgtgga caagtacgtc ggtgtttggt acgagctggc ctttcacgat
     4621 attacccagt gcaatggctg cggctgcacc cgttttaaca tgacgcgtca cggcaacatt
     4681 atcgaagata tgttcacggt gacgtgcccg tggccgtgga agaagggcgt cgatggtccg
     4741 tggctgccag gttacaatgc gaacggcaac cgcagaatga atatgtggac ttgcaacatg
     4801 accatgtatt acaaaccggc ggaaccgggc gtgatgctgg agacaggctt tggtcaagaa
     4861 ttcgataata tggtgttaga aatttggtcg gatccggaaa tcaccgcgga gacgggttac
     4921 gagtataccc gtgcgattca gtttcagtgc ctgggtagcg agcaggacgg catcaccttc
     4981 accggtatca actttttgtc gcgcatcccg attgtgaaaa gctctatgct gcaagaaatg
     5041 ttcaaccggg cccgtgcgct gggtctggag ccgtacggtt ccaatgatat gcatgttgtt
     5101 gagcacgctg gctgccgtta tccgcagagc accgacgaga catggatggg tgaacgtccg
     5161 gaatggccat tcccggtctt tgcgggtgat ttcggtgccc aaatctaaaa gcttcaaata
     5221 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     5281 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc
     5341 ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc
     5401 atcctgacgg atggcctttt tgcgtttcta caaactcttt cggtccgttg tttatttttc
     5461 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5521 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5581 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5641 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5701 cttgagagtt ttcgccccga agaacgtttc ccaatgatga gcacttttaa agttctgcta
     5761 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     5821 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5881 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5941 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6001 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6061 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6121 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6181 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6241 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6301 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6361 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6421 tatatacttt agattgattt ccttaggact gagcgtcaac cccgtagaaa agatcaaagg
     6481 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6541 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6601 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     6661 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6721 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6781 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6841 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6901 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6961 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7021 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7081 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7141 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7201 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     7261 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tataaggtgc
     7321 actgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     7381 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     7441 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat
     7501 cagcgtggtc gtgcagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga
     7561 gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt
     7621 tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga
     7681 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
     7741 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
     7801 tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc
     7861 agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt
     7921 ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg
     7981 ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag
     8041 taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc
     8101 gtggccagga cccaacgctg cccgaaatt
//