| 质粒名称: | pdCas9-DNMT3A-PuroR |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 71667 |
| 存储实验室: | Vlatka Zoldos |
|
载体骨架基本信息 |
|
| 载体名称: | PX462 |
| 空载体大小: | 4246 bp |
| 完整质粒大小: | 10152 bp |
| 载体类型: | 哺乳动物表达，CRISPR |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR，dCas9-DNMT3A-PuroRv,SpdCas9-DNMT3A-T2A-PuroR，dCas9-DNMT3A |
| 物种来源: | H. sapiens (human), Synthetic; S. pyogenes |
| 插入大小: | 5906 bp |
| 基因突变: | D10A and H840A in S.pyogenes Cas9 |
| GenBank编号 | AKA60242.1 NP_072046.2 |
| 启动子: | CBh |
| 标签/融合蛋白: |
3xFLAG（插入式N端子）
SV40 NLS（插入式N端子）
T2A PuroR（插入式C端子）
|
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI（未销毁） |
| 3’克隆位点: | FseI（未销毁） |
| 5’测序引物 | GS-linker For 5' GAAGAGGTACACCAGCACCAAAG 3' |
| 3’测序引物 | BGH Rev |
|
文献引用方法 |
|
| 材料和方法部分: | pdCas9-DNMT3A-PuroR was a gift from Vlatka Zoldos (Addgene plasmid # 71667 ; http://n2t.net/addgene:71667 ; RRID:Addgene_71667) |
| 参考文献部分: |
Repurposing the CRISPR-Cas9 system for targeted DNA methylation. Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, Zoldos V. Nucleic Acids Res. 2016 Mar 11. pii: gkw159. 10.1093/nar/gkw159 PubMed 26969735
|
买家导航
pdCas9-DNMT3A-PuroR载体质粒基本信息
pdCas9-DNMT3A-PuroR质粒图谱载体图谱和pdCas9-DNMT3A-PuroR载体序列质粒序列多克隆位点信息
pdCas9-DNMT3A-PuroR质粒载体简介
pdCas9-DNMT3A-PuroR质粒序列载体序列
LOCUS pdCas9-DNMT3A-PuroR 10151 bp DNA circular SYN 15-DEC-2025
DEFINITION Expression of human codon-optimized dCas9-DNMT3A fusion with
T2A-PuroR for targeted DNA methylation in mammalian cells; cloning
backbone for sgRNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10151)
AUTHORS Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M,
Zoldos V
TITLE Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
JOURNAL Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
PUBMED 26969735
REFERENCE 2 (bases 1 to 10151)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT Sequence Label: pdCas9-DNMT3A-PuroR
FEATURES Location/Qualifiers
source 1..10151
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..249
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA
(Domitrovich & Kunkel, 2003)"
primer_bind 1..21
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 172..191
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_RNA 268..343
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
enhancer 440..725
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter 727..1004
/label=chicken beta-actin promoter
intron 1005..1232
/label=hybrid intron
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
regulatory 1244..1253
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1253..1318
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 1325..1345
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS join(1370..1393,1394..1396,1397..3883,3884..3886,
3887..5470)
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 1370..5470
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5471..5518
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 5546..6478
/codon_start=1
/product="catalytic domain of DNA methyltransferase 3A"
/label=DNMT3A catalytic domain
/translation="PSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATG
LLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVI
GGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVS
DKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGR
IAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSR
LARQRLLGRSWSVPVIRHLFAPLKEYFACV"
CDS 6506..6559
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 6560..7159
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(6560..6579)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind complement(7192..7209)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 7198..7405
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 7414..7554
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 7414..7543
/label=AAV2 ITR
rep_origin 7629..8084
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7716..7735)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7926..7947
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(8101..8120)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 8220..8242
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(8280..8298)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 8366..8470
/gene="bla"
/label=AmpR promoter
CDS join(8471..8539,8540..9331)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8689..8708)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 9502..10090
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9991..10010
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
241 cgaaacaccg ggtcttcgag aagacctgtt ttagagctag aaatagcaag ttaaaataag
301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg ttttagagct
361 agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac
421 aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
481 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc
541 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tgtgcccagt
661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
721 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
781 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
841 ggggggggcg cgcgccgggg gggggggggg gggggggggg gggggggggg ggggggcgga
901 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
961 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
1021 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
1081 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
1141 tgagcaagag gtaagggttt aagggatggt tggttggtgg ggtattaatg tttaattacc
1201 tggagcacct gcctgaaatc actttttttc aggttggacc ggtgccacca tggactataa
1261 ggaccacgac ggagactaca aggatcatga tattgattac aaagacgatg acgataagat
1321 ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg acaagaagta
1381 cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta
1441 caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa
1501 gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa
1561 gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat
1621 cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt
1681 cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga
1741 ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag
1801 caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg
1861 gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt
1921 catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg
1981 cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct
2041 gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggcaacctga ttgccctgag
2101 cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca
2161 gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca
2221 gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat
2281 cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata
2341 cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga
2401 gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg
2461 cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg
2521 caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt
2581 cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg
2641 gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac
2701 cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat
2761 gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg
2821 cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga
2881 gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac
2941 caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa
3001 aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa
3061 agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga
3121 tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga
3181 cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact
3241 gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga
3301 caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa
3361 gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc
3421 cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa
3481 agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc
3541 caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga
3601 cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag
3661 agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga
3721 agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca
3781 gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga
3841 ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gacgctatcg tgcctcagag
3901 ctttctgaag gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg
3961 caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca
4021 gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag
4081 aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg
4141 gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga
4201 gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga
4261 tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca
4321 cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga
4381 aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag
4441 cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt
4501 tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac
4561 aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa
4621 agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt
4681 cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga
4741 ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt
4801 ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg
4861 gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa
4921 gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga
4981 gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga
5041 actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct
5101 gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta
5161 cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc
5221 taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca
5281 ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa
5341 gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc
5401 caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct
5461 gggaggcgac aaaaggccgg cggccacgaa aaaggccgga caggccaaaa agaaaaagct
5521 cgagggcgga ggcgggagcg gatccccctc ccggctccag atgttcttcg ctaataacca
5581 cgaccaggaa tttgaccctc caaaggttta cccacctgtc ccagctgaga agaggaagcc
5641 catccgggtg ctgtctctct ttgatggaat cgctacaggg ctcctggtgc tgaaggactt
5701 gggcattcag gtggaccgct acattgcctc ggaggtgtgt gaggactcca tcacggtggg
5761 catggtgcgg caccagggga agatcatgta cgtcggggac gtccgcagcg tcacacagaa
5821 gcatatccag gagtggggcc cattcgatct ggtgattggg ggcagtccct gcaatgacct
5881 ctccatcgtc aaccctgctc gcaagggcct ctacgagggc actggccggc tcttctttga
5941 gttctaccgc ctcctgcatg atgcgcggcc caaggaggga gatgatcgcc ccttcttctg
6001 gctctttgag aatgtggtgg ccatgggcgt tagtgacaag agggacatct cgcgatttct
6061 cgagtccaac cctgtgatga ttgatgccaa agaagtgtca gctgcacaca gggcccgcta
6121 cttctggggt aaccttcccg gtatgaacag gccgttggca tccactgtga atgataagct
6181 ggagctgcag gagtgtctgg agcatggcag gatagccaag ttcagcaaag tgaggaccat
6241 tactacgagg tcaaactcca taaagcaggg caaagaccag cattttcctg tgttcatgaa
6301 tgagaaagag gacatcttat ggtgcactga aatggaaagg gtatttggtt tcccagtcca
6361 ctatactgac gtctccaaca tgagccgctt ggcgaggcag agactgctgg gccggtcatg
6421 gagcgtgcca gtcatccgcc acctcttcgc tccgctgaag gagtattttg cgtgtgtgtc
6481 cggccggccc gaattcggca gtggagaggg cagaggaagt ctgctaacat gcggtgacgt
6541 cgaggagaat cctggcccaa tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
6601 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
6661 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
6721 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
6781 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
6841 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
6901 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
6961 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
7021 cggggtgccc gccttcctgg agacctccgc gccccacaac ctccccttct acgagcggct
7081 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
7141 ccgcaagccc ggtgcctgag aattcaattc taactagagc tcgctgatca gcctcgactg
7201 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
7261 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
7321 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
7381 aagagaatag caggcatgct ggggagcggc cgcaggaacc cctagtgatg gagttggcca
7441 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
7501 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
7561 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
7621 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
7681 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
7741 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
7801 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
7861 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
7921 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
7981 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
8041 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
8101 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
8161 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
8221 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
8281 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
8341 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
8401 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
8461 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
8521 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
8581 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
8641 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
8701 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
8761 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
8821 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
8881 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
8941 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
9001 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
9061 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
9121 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
9181 gcgtggaagc cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
9241 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
9301 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
9361 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
9421 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
9481 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
9541 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
9601 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
9661 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
9721 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
9781 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
9841 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
9901 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
9961 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
10021 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
10081 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
10141 tgctcacatg t
//
pdCas9-DNMT3A-PuroR载体图谱质粒图谱pdf版和pdCas9-DNMT3A-PuroR载体序列质粒序列等相关资料下载
pdCas9-DNMT3A-PuroR质粒载体应用举例
- 上一篇：pdCas9-DNMT3A-EGFP (ANV)
- 下一篇：没有了
