| 质粒名称: | pCgsgRNA_crtYf |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 153338 |
| 存储实验室: | Sheng Yang |
|
载体骨架基本信息 |
|
| 载体名称: | pJYS2_crtYf |
| 完整质粒大小: | 4214bp |
| 载体类型: | Bacterial Expression, CRISPR |
| 载体抗性: | Spectinomycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | crRNA targeting crtYf |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: | pCgsgRNA_crtYf was a gift from Sheng Yang (Addgene plasmid # 153338 ; http://n2t.net/addgene:153338 ; RRID:Addgene_153338) |
| 参考文献部分: | De Novo Engineering of Corynebacterium glutamicum for l-Proline Production. Zhang J, Qian F, Dong F, Wang Q, Yang J, Jiang Y, Yang S. ACS Synth Biol. 2020 Jul 17;9(7):1897-1906. doi: 10.1021/acssynbio.0c00249. Epub 2020 Jul 6. 10.1021/acssynbio.0c00249 PubMed 32627539 |
买家导航
pCgsgRNA_crtYf载体质粒基本信息
pCgsgRNA_crtYf质粒图谱载体图谱和pCgsgRNA_crtYf载体序列质粒序列多克隆位点信息
pCgsgRNA_crtYf质粒载体简介
pCgsgRNA_crtYf质粒序列载体序列
LOCUS pCgsgRNA_crtYf 4214 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Double-plasmid-based CRISPR-Cas9 system in Corynebacterium
glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA
targeting crtYf; Spcr.
ACCESSION .
VERSION .
KEYWORDS pCgsgRNA_crtYf
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4214)
AUTHORS Zhang J, Qian F, Dong F, Wang Q, Yang J, Jiang Y, Yang S
TITLE De Novo Engineering of Corynebacterium glutamicum for l-Proline
Production.
JOURNAL ACS Synth Biol. 2020 Jul 17;9(7):1897-1906. doi:
10.1021/acssynbio.0c00249. Epub 2020 Jul 6.
PUBMED 32627539
REFERENCE 2 (bases 1 to 4214)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4214
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_RNA 2259..2334
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind complement(2416..2433)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2587..3175)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2667..2686)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ataaactatt taaataacag attaaaaaaa ttataaaaaa attgaaaaaa tggtggaaac
61 acttttttca atttttttgt tttattattt aatatttggg aaatattcat tctaattggt
121 aatcagattt tagaaaacaa taaacccttg catatgatat cgatgtacag atcttttttt
181 aaggcagtta ttggtgccct tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
241 agatttcgat tccaccgccg ccttctatga aagccatcaa tcctgcctat ttgccacgtt
301 taacaaggta gttaagcgtt catttacgaa gaaaacacga taagctgcac aaatacctga
361 aaaagttgaa cgccccgtga gcgggaactc acagggcgtc ggctaacccc cagtcatcag
421 ctgggagaaa gcactcaaga catgactcta gccgatccgc aggacacagt cacagctagc
481 gcgtggaaat tttccgccga tctgttcgac acccaccccg aactagcgct gcgctcacgc
541 ggctggacgg cagaagatcg ccgcgaactg ctcgctcacc tgggacgcga aagcttccag
601 ggcagcaaga caagagattt cgcgagcgcc tggattaaaa acccggatac cggcgaaacc
661 caaccaaagc tctaccgggc tggctcaaaa gcgctgacgc ggtgccagta cgttgcgctg
721 acgcacgcgc aacatgccgc ggtgatcgtg cttgacatcg atgtgcccag ccaccaggcc
781 ggcgggaaga ttgagcacgt aaacccgcag gtctacgcga ttttagagaa atgggcacgc
841 ctagaaaaag cgccggcttg gatcggcgtg aatccgctga gcgggaaatg ccagctcatc
901 tggctcattg acccggtgta tgccgcagca ggtaaaacca gcccaaatat gcgcctgctg
961 gctgcaacga cggaagaaat gactcgtgtt ttcggcgctg accaggcttt ttcgcatagg
1021 ctgagccggt ggccgctgca cgtctcagac gatccgacag cctataaatg gcactgccag
1081 catgatcgtg tggatcggct ggccgaccta atggagattg ctcgaacgat gaccggatca
1141 cagaagccga aaaagtacat tgagcaggac ttttccagcg gacgcgcccg cattgaagcg
1201 gcacaacgcg ccaccgcaga agccaaggcg ctagcgattt tggacgcgag cctgccgagc
1261 gccctggacg cgtccggcga cctgatcgac ggcgtgcgag tgctctggac aaatccagag
1321 cgcgcagcgc gcgacgagac cgcgtttcgc cacgcgttga ccgtgggata ccagctcaaa
1381 gctgctggtg agcgcctaaa agatgccaag atcatcgacg cgtatgaagt ggcgtacaac
1441 gttgcccagg cggtcggtgc agacggccgg gagccggatc ttcccgccat gcgtgatcgc
1501 ctgacgatgg cgcgtcgtgt gcgcggctac gtggctaaag gccagccagt cgtccctgct
1561 cgtcgggtgg aaacgcagag cagccgaggg cggaaagctc tagcgacgat ggggcgacgg
1621 ggcgcagcta catcgaatgc acgcagatgg gctgacccag aaagtaagta tgcgcaggag
1681 acgcgacagc gattagcgga agcaaacaaa cgccgagaaa tgacaggcga gttgctcgaa
1741 cttcgcgtca aaactgcgat cctggatgcc cgttctcaat cggttgctga tccctcgact
1801 cgtgagcttg caggcgaact aggtgtcagt gaaaggcgca tccaacaagt cagaaaggca
1861 cttggaatgg aagctaaacg cggccgtcca cgggctgaaa actaataaac gaaacaccgt
1921 cagcagaaaa cggttccccc ctttaggggt cccgtccttg ctctggctct cacttgccct
1981 caccctccgc tatccacggg ctgaaaacta ataaacgaaa caccgtcagc agaaaacggt
2041 tccccccctt tagggtgtct cgctcctagc tctgatccct ccccggttcc tccccggcct
2101 gatttttaag ggggctcacg ctgtcggcag agaacggttc ccccgccttc tgctctggct
2161 cttcctcgac tccctccccc tcaaaaatct cctcgagtct agattgacag ctagctcagt
2221 cctaggtata atggatccct gcgtttaaac atatttccgt tttagagcta gaaatagcaa
2281 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
2341 taagcttcat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
2401 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
2461 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
2521 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
2581 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
2641 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
2701 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
2761 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
2821 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
2881 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
2941 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
3001 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
3061 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
3121 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
3181 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
3241 tttggtcatg aactaataac gtaacgtgac tggcaagaga tatttttaaa acaatgaata
3301 ggtttacact tactttagtt ttatggaaat gaaagatcat atcatatata atctagaata
3361 aaattaacta aaataattat tatctagata aaaaatttag aagccaatga aatctataaa
3421 taaactaaat taagtttatt taattaacaa ctatggatat aaaataggta ctaatcaaaa
3481 tagtgaggag gatatatttg aatacatacg aacaaattaa taaagtgaaa aaaatacttc
3541 ggaaacattt aaaaaataac cttattggta cttacatgtt tggatcagga gttgagagtg
3601 gactaaaacc aaatagtgat cttgactttt tagtcgtcgt atctgaacca ttgacagatc
3661 aaagtaaaga aatacttata caaaaaatta gacctatttc aaaaaaaata ggagataaaa
3721 gcaacttacg atatattgaa ttaacaatta ttattcagca agaaatggta ccgtggaatc
3781 atcctcccaa acaagaattt atttatggag aatggttaca agagctttat gaacaaggat
3841 acattcctca gaaggaatta aattcagatt taaccataat gctttaccaa gcaaaacgaa
3901 aaaataaaag aatatacgga aattatgact tagaggaatt actacctgat attccatttt
3961 ctgatgtgag aagagccatt atggattcgt cagaggaatt aatagataat tatcaggatg
4021 atgaaaccaa ctctatatta actttatgcc gtatgatttt aactatggac acgggtaaaa
4081 tcataccaaa agatattgcg ggaaatgcag tggctgaatc ttctccatta gaacataggg
4141 agagaatttt gttagcagtt cgtagttatc ttggagagaa tattgaatgg actaatgaaa
4201 atgtaaattt aact
//
