| 质粒名称: | pINDUCER21-puro_Gateway-3xFLAG |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 172981 |
| 存储实验室: | Michael Wehr |
|
载体骨架基本信息 |
|
| 载体名称: | pINDUCER21 |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 12131bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | ccdB Survival |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | 无 |
| 标签/融合蛋白: | 3xFLAG (C terminal on backbone) |
|
文献引用方法 |
|
| 材料和方法部分: | pINDUCER21-puro_Gateway-3xFLAG was a gift from Michael Wehr (Addgene plasmid # 172981 ; http://n2t.net/addgene:172981 ; RRID:Addgene_172981) |
| 参考文献部分: | Comprehensive split TEV based protein-protein interaction screening reveals TAOK2 as a key modulator of Hippo signalling to limit growth. Ma X, Mandausch FJ, Wu Y, Sahoo VK, Ma W, Leoni G, Hostiuc M, Wintgens JP, Qiu J, Kannaiyan N, Rossner MJ, Wehr MC. Cell Signal. 2024 Jan;113:110917. doi: 10.1016/j.cellsig.2023.110917. Epub 2023 Oct 7. 10.1016/j.cellsig.2023.110917 PubMed 37813295 |
买家导航
pINDUCER21-puro_Gateway-3xFLAG载体质粒基本信息
pINDUCER21-puro_Gateway-3xFLAG质粒图谱载体图谱和 pINDUCER21-puro_Gateway-3xFLAG载体序列质粒序列多克隆位点信息
pINDUCER21-puro_Gateway-3xFLAG质粒载体简介
pINDUCER21-puro_Gateway-3xFLAG质粒序列载体序列
LOCUS pINDUCER21-puro_Gateway-3xFLAG 12131 bp DNA circular SYN 14-APR-2025
DEFINITION Gateway Destination vector for doxycycline-inducible expression of
ORF.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12131)
AUTHORS Ma X, Mandausch FJ, Wu Y, Sahoo VK, Ma W, Leoni G, Hostiuc M,
Wintgens JP, Qiu J, Kannaiyan N, Rossner MJ, Wehr MC
TITLE Comprehensive split TEV based protein-protein interaction screening
reveals TAOK2 as a key modulator of Hippo signalling to limit
growth.
JOURNAL Cell Signal. 2024 Jan;113:110917. doi:
10.1016/j.cellsig.2023.110917. Epub 2023 Oct 7.
PUBMED 37813295
REFERENCE 2 (bases 1 to 12131)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cell
Signal."; date: "2024-01"; volume: "113:110917. doi"; pages: "
10.1016/j.cellsig.2023.110917. Epub 2023 Oct 7"
COMMENT Sequence Label: pINDUCER21-puro_Gateway-3xFLAG
FEATURES Location/Qualifiers
source 1..12131
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2058..2175
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
2440..2458,2459..2481,2482..2500)
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 2533..2571
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2568..2592
/label=LNCX
/note="Human CMV promoter, forward primer"
protein_bind 2701..2825
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter join(2850..2855,2856..2873,2874..2880)
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 2934..3593
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(3000..3019)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 3935..4240
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 4119..4138
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(4281..4405)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS 4458..4523
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 4541..4567
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
promoter 4787..5965
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 5018..5956
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 5913..5933
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
protein_bind 6035..6068
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
CDS join(6075..6692,6693..6698,6699..6734,6735..6737,6738..6773,
6774..6776,6777..6812,6813..6821)
/codon_start=1
/product="improved tetracycline-controlled transactivator"
/label=rtTA-Advanced
/note="In the Tet-On(R) system, rtTA-Advanced binds to the
Tet-responsive element and stimulates transcription only in
the presence of tetracycline or doxycycline."
/translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
misc_feature join(6874..7447,7448..7450,7451..7459)
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(7041..7058)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 7268..7287
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 7479..8078
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(7479..7498)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 7975..7995
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind 8214..8233
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
misc_feature 8521..9109
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8574..8594)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8992..9003)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 9184..9417
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(9536..9554)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 9622..9726
/gene="bla"
/label=AmpR promoter
CDS join(9727..9795,9796..10587)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(9945..9964)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 10758..11346
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 11247..11266
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 11500..11517
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 11757..11778
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter join(11793..11798,11799..11816,11817..11823)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11831..11847
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 11836..11858
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 11855..11871
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 11855..11871
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 11921..11940
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
2701 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
2761 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
2821 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
2881 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
2941 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
3001 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
3061 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
3121 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
3181 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
3241 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
3301 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
3361 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
3421 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
3481 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
3541 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg
3601 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
3661 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
3721 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
3781 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
3841 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
3901 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
3961 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
4021 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
4081 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
4141 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
4201 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
4261 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
4321 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
4381 gttcagcttt cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag
4441 agggcccgcg gttcgaagac tacaaagacc atgacggtga ttataaagat catgacatcg
4501 attacaagga tgacgatgac aagtaggtaa gtgattcgaa tacccatacg acgtcccaga
4561 ctacgcttag taatgattaa ttaaactaga aattctaccg ggtaggggag gcgcttttcc
4621 caaggcagtc tggagtaacc cacccaagat ctggcctccg cgccgggttt tggcgcctcc
4681 cgcgggcgcc cccctcctca cggcgagccg cgtaagatct ggcctccgcg ccgggttttg
4741 gcgcctcccg cgggcgcccc cctcctcacg gcgagcctag tcgtgaggct ccggtgcccg
4801 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
4861 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
4921 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
4981 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
5041 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
5101 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
5161 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
5221 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
5281 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
5341 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
5401 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
5461 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
5521 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
5581 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
5641 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
5701 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
5761 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
5821 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
5881 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
5941 ttttcttcca tttcaggtgt cgtgaagcgg ccgccatgct agtaaattgt ccgctaaatt
6001 ctggccgttt ttggcttttt tgttagacgg atccataact tcgtataatg tatgctatac
6061 gaagttatgc caccatgtct agactggaca agagcaaagt cataaacgga gctctggaat
6121 tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag
6181 ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc
6241 caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc
6301 aagactttct gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg
6361 gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc
6421 agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg
6481 ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag
6541 aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc
6601 tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg
6661 gcctggagaa acagctaaag tgcgaaagcg gcgggccgac cgacgccctt gacgattttg
6721 acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg
6781 acgctcttga cgattttgac cttgacatgc tccccgggta actaagtaag gatccgcggc
6841 cgcactagag gaattccgcc cctctccccc ccccccctct ccctcccccc cccctaacgt
6901 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
6961 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
7021 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
7081 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
7141 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
7201 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
7261 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
7321 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
7381 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttg aaaaacacga
7441 tgataagctt gccacaaccc acaaggagac gaccttccat gaccgagtac aagcccacgg
7501 tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg
7561 ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg
7621 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
7681 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
7741 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
7801 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
7861 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
7921 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
7981 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
8041 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc atgcccgacg gcgaggatct
8101 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
8161 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
8221 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
8281 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
8341 ctgatgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag
8401 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
8461 atgcaattgt tgttgtttag tccctcccaa ttcgatatca agcttatcgc gatagatcct
8521 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
8581 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
8641 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
8701 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
8761 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
8821 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
8881 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
8941 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
9001 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
9061 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt
9121 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
9181 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
9241 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
9301 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
9361 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
9421 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
9481 gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc
9541 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
9601 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
9661 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
9721 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
9781 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
9841 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
9901 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
9961 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
10021 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
10081 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
10141 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
10201 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
10261 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
10321 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
10381 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
10441 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
10501 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
10561 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
10621 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
10681 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
10741 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
10801 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
10861 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
10921 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
10981 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
11041 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
11101 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
11161 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
11221 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
11281 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
11341 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
11401 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
11461 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
11521 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
11581 agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
11641 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
11701 tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
11761 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
11821 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg
11881 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
11941 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
12001 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
12061 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
12121 aacttagtag t
//
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pINDUCER21-puro_Gateway-3xFLAG质粒载体应用举例
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