pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DCC-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DCC-FLAG         7171 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3884
                     /codon_start=1
                     /gene="DCC"
                     /gene_synonym="CRC18; CRCR1; HGPPS2; IGDCC1; MRMV1;NTN1R1"
                     /product="netrin receptor DCC"
                     /label=DCC
                     /note="colorectal cancer suppressor; tumor 
                     suppressorprotein DCC; immunoglobulin superfamily DCC 
                     subclass member 1; deleted in colorectal carcinoma; 
                     putative colorectal tumor suppressor"
                     /db_xref="GI:110431348"
                     /db_xref="CCDS:CCDS11952.1"
                     /db_xref="GeneID:1630"
                     /db_xref="HGNC:HGNC:2701"
                     /db_xref="MIM:120470"
                     /protein_id="NP_005206.2"
                     /translation="GEMETLEPNNLWYLFTGLEKGSQYSFQVSAMTVNGTGPPSNWYTA
                     ETPENDLDESQVPDQPSSLHVRPQTNCIIMSWTPPLNPNIVVRGYIIGYGVGSPYAETV
                     RVDSKQRYYSIERLESSSHYVISLKAFNNAGEGVPLYESATTRSITDPTDPVDYYPLLD
                     DFPTSVPDLSTPMLPPVGVQAVALTHDAVRVSWADNSVPKNQKTSEVRLYTVRWRTSFS
                     ASAKYKSEDTTSLSYTATGLKPNTMYEFSVMVTKNRRSSTWSMTAHATTYEAAPTSAPK
                     DLTVITREGKPRAVIVSWQPPLEANGKITAYILFYTLDKNIPIDDWIMETISGDRLTHQ
                     IMDLNLDTMYYFRIQARNSKGVGPLSDPILFRTLKVEHPDKMANDQGRHGDGGYWPVDT
                     NLIDRSTLNEPPIGQMHPPHGSVTPQKNSNLLVIIVVTVGVITVLVVVIVAVICTRRSS
                     AQQRKKRATHSAGKRKGSQKDLRPPDLWIHHEEMEMKNIEKPSGTDPAGRDSPIQSCQD
                     LTPVSHSQSETQLGSKSTSHSGQDTEEAGSSMSTLERSLAARRAPRAKLMIPMDAQSNN
                     PAVVSAIPVPTLESAQYPGILPSPTCGYPHPQFTLRPVPFPTLSVDRGFGAGRSQSVSE
                     GPTTQQPPMLPPSQPEHSSSEEAPSRTIPTACVRPTHPLRSFANPLLPPPMSAIEPKVP
                     YTPLLSQPGPTLPKTHVKTASLGLAGKARSPLLPVSVPTAPEVSEESHKPTEDSANVYE
                     QDDLSEQMASLEGLMKQLNAITGSAF"
     misc_feature    3514..3519
                     /label=SDS
     CDS             3891..3956
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3960..4391
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4104..4109
                     /label=SDS
     repeat_region   4434..4574
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4434..4563
                     /label=AAV2 ITR
     repeat_region   4435..4575
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4649..5104
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5386..5490
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5491..6351
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6522..7110
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggggt gagatggaaa
     1561 cactggagcc aaacaacctc tggtacctat tcacaggact ggagaaagga agtcagtaca
     1621 gtttccaggt gtcagccatg acagtcaatg gtactggacc accttccaac tggtatactg
     1681 cagagactcc agagaatgat ctagatgaat ctcaagttcc tgatcaacca agctctcttc
     1741 atgtgaggcc ccagactaac tgcatcatca tgagttggac tcctcccttg aacccaaaca
     1801 tcgtggtgcg aggttatatt atcggttatg gcgttgggag cccttacgct gagacagtgc
     1861 gtgtggacag caagcagcga tattattcca ttgagaggtt agagtcaagt tcccattatg
     1921 taatctccct aaaagctttt aacaatgccg gagaaggagt tcctctttat gaaagtgcca
     1981 ccaccaggtc tataaccgat cccactgacc cagttgatta ttatcctttg cttgatgatt
     2041 tccccacctc ggtcccagat ctctccaccc ccatgctccc accagtaggt gtacaggctg
     2101 tggctcttac ccatgatgct gtgagggtca gctgggcaga caactctgtc cctaagaacc
     2161 aaaagacgtc tgaggtgcga ctttacaccg tccggtggag aaccagcttt tctgcaagtg
     2221 caaaatacaa gtcagaagac acaacatctc taagttacac agcaacaggc ctcaaaccaa
     2281 acacaatgta tgaattctcg gtcatggtaa caaaaaacag aaggtccagt acttggagca
     2341 tgactgcaca tgccaccacg tatgaagcag cccccacctc tgctcccaag gacttgacag
     2401 tcattactag ggaagggaag cctcgtgccg tcattgtgag ttggcagcct cccttggaag
     2461 ccaatgggaa aattactgct tacatcttat tttatacctt ggacaagaac atcccaattg
     2521 atgactggat tatggaaaca atcagtggtg ataggcttac tcatcaaatc atggatctca
     2581 accttgatac tatgtattac tttcgaattc aagcacgaaa ttcaaaagga gtggggccac
     2641 tctctgatcc tatcctcttc aggactctga aagtggaaca ccctgacaaa atggctaatg
     2701 accaaggtcg tcatggagat ggaggttatt ggccagttga tactaatttg attgatagaa
     2761 gcaccctaaa tgagccgcca attggacaaa tgcacccccc gcatggcagt gtcactcctc
     2821 agaagaacag caacctgctt gtgatcattg tggtcaccgt tggtgtcatc acagtgctgg
     2881 tagtggtcat cgtggctgtg atttgcaccc gacgctcttc agcccagcag agaaagaaac
     2941 gggccaccca cagtgctggc aaaaggaagg gcagccagaa ggacctccga ccccctgatc
     3001 tttggatcca tcatgaagaa atggagatga aaaatattga aaagccatct ggcactgacc
     3061 ctgcaggaag ggactctccc atccaaagtt gccaagacct cacaccagtc agccacagcc
     3121 agtcagaaac ccaactggga agcaaaagca cctctcattc aggtcaagac actgaggaag
     3181 cagggagctc tatgtccact ctggagaggt cgctggctgc acgccgagcc ccccgggcca
     3241 agctcatgat tcccatggat gcccagtcca acaatcctgc tgtcgtgagc gccatcccgg
     3301 tgccaacgct agaaagtgcc cagtacccag gaatcctccc gtctcccacc tgtggatatc
     3361 cccacccgca gttcactctc cggcctgtgc cattcccaac actctcagtg gaccgaggtt
     3421 tcggagcagg aagaagtcag tcagtgagtg aaggaccaac tacccaacaa ccacctatgc
     3481 tgcccccatc tcagcctgag cattctagca gcgaggaggc accaagcaga accatcccca
     3541 cagcttgtgt tcgaccaact cacccactcc gcagctttgc taatcctttg ctacctccac
     3601 caatgagtgc aatagaaccg aaagtccctt acacaccact tttgtctcag ccagggccca
     3661 ctcttcctaa gacccatgtg aaaacagcct cccttgggtt ggctggaaaa gcaagatccc
     3721 ctttgcttcc tgtgtctgtg ccaacagccc ctgaagtgtc tgaggagagc cacaaaccaa
     3781 cagaggattc agccaatgtg tatgaacagg atgatctgag tgaacaaatg gcaagtttgg
     3841 aaggactcat gaagcagctt aatgccatca caggctcagc cttttgtaca gactacaaag
     3901 accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtaac
     3961 tcgagataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     4021 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     4081 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttagttc ttgccacggc
     4141 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4201 caattccgtg gtgtttattt gtgaaatttg tgatgctatt gctttatttg taaccatcta
     4261 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     4321 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg
     4381 aggtttttta aagatctgga gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc
     4441 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     4501 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     4561 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     4621 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     4681 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
     4741 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4801 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4861 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4921 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4981 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
     5041 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     5101 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     5161 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     5221 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     5281 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5341 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5401 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5461 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5521 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5581 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5641 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5701 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5761 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5821 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5881 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5941 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6001 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6061 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6121 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6181 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6241 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6301 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6361 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6421 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6481 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6541 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6601 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6661 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6721 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6781 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6841 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6901 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6961 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7021 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7081 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7141 ctggcctttt gctggccttt tgctcacatg t
//