pCXLE-gw

LOCUS       pCXLE-gw               11898 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  Non-integrating (episomal) expression vector with Gateway cassette.
ACCESSION   .
VERSION     .
KEYWORDS    pCXLE-gw
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11898)
  AUTHORS   Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, 
            Goshima N, Yamanaka S
  TITLE     An Efficient Non-viral Method to Generate Integration-Free Human iPS
            Cells from Cord Blood and Peripheral Blood Cells.
  JOURNAL   Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
  PUBMED    23193063
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pCXLE-gw|
FEATURES             Location/Qualifiers
     source          1..11898
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    31..619
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(84..104)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(502..513)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(709..728)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_site      774..829
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(828..847)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1190..1206)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1190..1206)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1203..1225)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1214..1230
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1238..1268)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1283..1304
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    complement(1393..1426)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             1887..3812
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    complement(3980..4013)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      4200..5989
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(6531..6548)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6702..7290)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6782..6801)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7461..8321)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8084..8103
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8322..8426)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8457..8836
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8838..9114
                     /label=chicken beta-actin promoter
     intron          9115..10131
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     10055..10077
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     10139..10158
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    10190..10314
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        10339..10369
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             10423..11103
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(10489..10508)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             11423..11728
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     11607..11626
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(11769..11893)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 aattcaagct tcggggacta gtcatatgat aatcaacctc tggattacaa aatttgtgaa
       61 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
      121 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
      181 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
      241 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
      301 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
      361 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
      421 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
      481 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
      541 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
      601 ctttgggccg cctccccgca tcggtaaatt cactcctcag gtgcaggctg cctatcagaa
      661 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
      721 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
      781 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
      841 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
      901 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
      961 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     1021 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     1081 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     1141 tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt
     1201 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     1261 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1321 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ctcaattccg atcatattca
     1381 ataaccctta atataacttc gtataatgta tgctatacga agttattagg tctgaagagg
     1441 agtttacgtc cagccaagct taggatcaat tctcatgttt gacagcttat catcgataag
     1501 ctgatcctca caggccgcac ccagcttttc ttccgttgcc ccagtagcat ctctgtctgg
     1561 tgaccttgaa gaggaagagg aggggtcccg agaatcccca tccctaccgt ccagcaaaaa
     1621 gggggacgag gaatttgagg cctggcttga ggctcaggac gcaaatcttg aggatgttca
     1681 gcgggagttt tccgggctgc gagtaattgg tgatgaggac gaggatggtt cggaggatgg
     1741 ggaattttca gacctggatc tgtctgacag cgaccatgaa ggggatgagg gtgggggggc
     1801 tgttggaggg ggcaggagtc tgcactccct gtattcactg agcgtcgtct aataaagatg
     1861 tctattgatc tcttttagtg tgaatcatgt ctgacgaggg gccaggtaca ggacctggaa
     1921 atggcctagg agagaaggga gacacatctg gaccagaagg ctccggcggc agtggacctc
     1981 aaagaagagg gggtgataac catggacgag gacggggaag aggacgagga cgaggaggcg
     2041 gaagaccagg agccccgggc ggctcaggat cagggccaag acatagagat ggtgtccgga
     2101 gaccccaaaa acgtccaagt tgcattggct gcaaagggac ccacggtgga acaggagcag
     2161 gagcaggagc gggaggggca ggagcaggag gggcaggagc aggaggaggg gcaggagcag
     2221 gaggaggggc aggaggggca ggaggggcag gaggggcagg agcaggagga ggggcaggag
     2281 caggaggagg ggcaggaggg gcaggagggg caggagcagg aggaggggca ggagcaggag
     2341 gaggggcagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagcag
     2401 gaggaggggc aggagcagga ggaggggcag gaggggcagg agcaggagga ggggcaggag
     2461 gggcaggagg ggcaggagca ggaggagggg caggagcagg aggggcagga ggggcaggag
     2521 gggcaggagc aggaggggca ggagcaggag gaggggcagg aggggcagga ggggcaggag
     2581 caggaggggc aggagcagga ggggcaggag caggaggggc aggagcagga ggggcaggag
     2641 gggcaggagc aggaggggca ggaggggcag gagcaggagg ggcaggaggg gcaggagcag
     2701 gaggaggggc aggaggggca ggagcaggag gaggggcagg aggggcagga gcaggagggg
     2761 caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggggca ggaggggcag
     2821 gagcaggagg aggggcagga gcaggagggg caggagcagg aggtggaggc cggggtcgag
     2881 gaggcagtgg aggccggggt cgaggaggta gtggaggccg gggtcgagga ggtagtggag
     2941 gccgccgggg tagaggacgt gaaagagcca gggggggaag tcgtgaaaga gccaggggga
     3001 gaggtcgtgg acgtggagaa aagaggccca ggagtcccag tagtcagtca tcatcatccg
     3061 ggtctccacc gcgcaggccc cctccaggta gaaggccatt tttccaccct gtaggggaag
     3121 ccgattattt tgaataccac caagaaggtg gcccagatgg tgagcctgac gtgcccccgg
     3181 gagcgataga gcagggcccc gcagatgacc caggagaagg cccaagcact ggaccccggg
     3241 gtcagggtga tggaggcagg cgcaaaaaag gagggtggtt tggaaagcat cgtggtcaag
     3301 gaggttccaa cccgaaattt gagaacattg cagaaggttt aagagctctc ctggctagga
     3361 gtcacgtaga aaggactacc gacgaaggaa cttgggtcgc cggtgtgttc gtatatggag
     3421 gtagtaagac ctccctttac aacctaaggc gaggaactgc ccttgctatt ccacaatgtc
     3481 gtcttacacc attgagtcgt ctcccctttg gaatggcccc tggacccggc ccacaacctg
     3541 gcccgctaag ggagtccatt gtctgttatt tcatggtctt tttacaaact catatatttg
     3601 ctgaggtttt gaaggatgcg attaaggacc ttgttatgac aaagcccgct cctacctgca
     3661 atatcagggt gactgtgtgc agctttgacg atggagtaga tttgcctccc tggtttccac
     3721 ctatggtgga aggggctgcc gcggagggtg atgacggaga tgacggagat gaaggaggtg
     3781 atggagatga gggtgaggaa gggcaggagt gatgtaactt gttaggagac gccctcaatc
     3841 gtattaaaag ccgtgtattc ccccgcacta aagaataaat ccccagtaga catcatgcgt
     3901 gctgttggtg tatttctggc catctgtctt gtcaccattt tcgtcctccc aacatggggc
     3961 aattgccgga acccttaata taacttcgta taatgtatgc tatacgaagt tattaggtcc
     4021 ctcgaagagg ttcactagcg gatctcaatt gggcataccc atgttgtcac gtcactcagc
     4081 tccgcgctca acaccttctc gcgttggaaa acattagcga catttacctg gtgagcaatc
     4141 agacatgcga cggctttagc ctggcctcct taaattcacc taagaatggg agcaaccagc
     4201 aggaaaagga caagcagcga aaattcacgc ccccttggga ggtggcggca tatgcaaagg
     4261 atagcactcc cactctacta ctgggtatca tatgctgact gtatatgcat gaggatagca
     4321 tatgctaccc ggatacagat taggatagca tatactaccc agatatagat taggatagca
     4381 tatgctaccc agatatagat taggatagcc tatgctaccc agatataaat taggatagca
     4441 tatactaccc agatatagat taggatagca tatgctaccc agatatagat taggatagcc
     4501 tatgctaccc agatatagat taggatagca tatgctaccc agatatagat taggatagca
     4561 tatgctatcc agatatttgg gtagtatatg ctacccagat ataaattagg atagcatata
     4621 ctaccctaat ctctattagg atagcatatg ctacccggat acagattagg atagcatata
     4681 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcctatg
     4741 ctacccagat ataaattagg atagcatata ctacccagat atagattagg atagcatatg
     4801 ctacccagat atagattagg atagcctatg ctacccagat atagattagg atagcatatg
     4861 ctatccagat atttgggtag tatatgctac ccatggcaac attagcccac cgtgctctca
     4921 gcgacctcgt gaatatgagg accaacaacc ctgtgcttgg cgctcaggcg caagtgtgtg
     4981 taatttgtcc tccagatcgc agcaatcgcg cccctatctt ggcccgccca cctacttatg
     5041 caggtattcc ccggggtgcc attagtggtt ttgtgggcaa gtggtttgac cgcagtggtt
     5101 agcggggtta caatcagcca agttattaca cccttatttt acagtccaaa accgcagggc
     5161 ggcgtgtggg ggctgacgcg tgcccccact ccacaatttc aaaaaaaaga gtggccactt
     5221 gtctttgttt atgggcccca ttggcgtgga gccccgttta attttcgggg gtgttagaga
     5281 caaccagtgg agtccgctgc tgtcggcgtc cactctcttt ccccttgtta caaatagagt
     5341 gtaacaacat ggttcacctg tcttggtccc tgcctgggac acatcttaat aaccccagta
     5401 tcatattgca ctaggattat gtgttgccca tagccataaa ttcgtgtgag atggacatcc
     5461 agtctttacg gcttgtcccc accccatgga tttctattgt taaagatatt cagaatgttt
     5521 cattcctaca ctagtattta ttgcccaagg ggtttgtgag ggttatattg gtgtcatagc
     5581 acaatgccac cactgaaccc cccgtccaaa ttttattctg ggggcgtcac ctgaaacctt
     5641 gttttcgagc acctcacata caccttactg ttcacaactc agcagttatt ctattagcta
     5701 aacgaaggag aatgaagaag caggcgaaga ttcaggagag ttcactgccc gctccttgat
     5761 cttcagccac tgcccttgtg actaaaatgg ttcactaccc tcgtggaatc ctgaccccat
     5821 gtaaataaaa ccgtgacagc tcatggggtg ggagatatcg ctgttcctta ggaccctttt
     5881 actaacccta attcgatagc atatgcttcc cgttgggtaa catatgctat tgaattaggg
     5941 ttagtctgga tagtatatac tactacccgg gaagcatatg ctacccgttt agggttaaca
     6001 agggggcctt ataaacacta ttgctaatgc cctcttgagg gtccgcttat cggtagctac
     6061 acaggcccct ctgattgacg ttggtgtagc ctcccgtagt cttcctgggc ccctgggagg
     6121 tacatgtccc ccagcattgg tgtaagagct tcagccaaga gttacacata aaggcaatgt
     6181 tgtgttgcag tccacagact gcaaagtctg ctccaggatg aaagccactc agtgttggca
     6241 aatgtgcaca tccatttata aggatgtcaa ctacagtcag agaacccctt tgtgtttggt
     6301 ccccccccgt gtcacatgtg gaacagggcc cagttggcaa gttgtaccaa ccaactgaag
     6361 ggattacatg cactgccccg cgaagaaggg gcagagatgt cgtagtcagg tttagttcgt
     6421 ccggggcggg gcatcgatcc tctagagtcg acgctagcgg atccgctgca ttaatgaatc
     6481 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6661 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6721 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6781 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6841 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6901 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6961 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7021 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7081 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7141 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7201 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7261 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7321 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7381 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7441 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7501 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7561 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7621 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7681 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7741 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7801 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7861 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7921 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7981 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8041 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8101 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8161 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8221 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8281 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8341 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8401 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgaca
     8461 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     8521 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     8581 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     8641 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     8701 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     8761 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     8821 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
     8881 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
     8941 ggcggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg
     9001 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     9061 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     9121 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
     9181 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
     9241 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
     9301 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
     9361 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
     9421 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
     9481 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     9541 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
     9601 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
     9661 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     9721 cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt
     9781 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     9841 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
     9901 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     9961 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
    10021 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
    10081 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
    10141 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattatcaaa caagtttgta
    10201 caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
    10261 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcct atgggcggcc
    10321 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
    10381 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
    10441 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
    10501 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
    10561 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
    10621 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
    10681 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
    10741 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
    10801 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
    10861 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
    10921 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
    10981 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
    11041 gaattacaac agtactgcga tgagtggcag gcggggcgta atctagagga tccggcttac
    11101 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
    11161 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
    11221 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
    11281 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
    11341 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
    11401 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
    11461 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
    11521 gatccccctg gccagtgcac gtctgctgtc agataaagtc ccccgtgaac tttacccggt
    11581 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
    11641 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
    11701 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
    11761 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
    11821 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
    11881 tgtacaaagt ggttcgat
//