pL

LOCUS       pL               16708 bp ds-DNA     circular SYN 12-APR-2023
DEFINITION  35S promoter-driven expression of the positive-sense TSWV L RNA 
            segment encoding codon-optimized L (RdRp).
ACCESSION   .
VERSION     .
KEYWORDS    pL
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16708)
  AUTHORS   Liu Q, Zhao C, Sun K, Deng Y, Li Z
  TITLE     Engineered biocontainable RNA virus vectors for non-transgenic 
            genome editing across crop species and genotypes
  JOURNAL   Molecular Plant
REFERENCE   2  (bases 1 to 16708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 12, 2023 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..16708
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        423..768
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     711..731
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      9777..10029
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10077..10101
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      10229..11571
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(11192..11239)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11612..11630
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11668..11690)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11790..11809
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        11830..12111
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             12112..12786
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(12889..13344)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13026..13047)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13238..13257
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(13460..14608)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(14907..15701)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      15910..16541
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    16621..16645
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca cacttgtcta ctccaaaaat
       61 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
      121 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
      181 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
      241 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgataa catggtggag
      361 cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca
      421 attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct
      481 atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat
      541 tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga
      601 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
      661 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
      721 caagaccctt cctctatata aggaagttca tttcatttgg agaggagagc aatcaggtaa
      781 caacgatttt aagcaaacat gaacatccag aaaatccaga agctgattga gaacggaact
      841 acacttctgt tgtctattga ggattgcgtg ggcagcaact acgatcttgc tcttgatctc
      901 cacaagcgga actccgatga gattcctgag gacgtgatca tcaacaacaa cgccaagaac
      961 tacgagacta tgcgtgagct gatcgtgaag atcactgctg atggtgaggg tctgaacaaa
     1021 ggtatggcta ccgtggacgt gaagaaactg tctgagatgg tgtctctgtt cgagcagaag
     1081 taccttgaga ctgagcttgc taggcacgat atcttcggcg agcttatttc taggcacctg
     1141 aggatcaagc cgaagcagag gtcagaggtc gagatcgagc acgccctgag ggagtacctg
     1201 gacgagctca acaagaaatc ctgcatcaac aagctgagcg acgacgagtt cgagcggatc
     1261 aacaaagaat atgttgcaac caatgctaca ccagacaatt acgttatcta caaggaaagt
     1321 aagaatagcg agctttgcct gatcatctac gactggaaga tttctgtgga tgccaggact
     1381 gagactaaga ccatggaaaa gtactacaag aacatctgga agtccttcaa ggacatcaaa
     1441 gtcaacggca agcccttcct ggaggaccac ccggtcttcg tgagcatcgt gatcctgaag
     1501 cctattgctg gcatgcctat taccgtgacc tctagcagag tgctcgagaa gtttgaggac
     1561 tctccttctg cacttcacgg cgagaggatt aagcacgcta ggaacgctaa gctgctgaac
     1621 atttctcacg tgggtcagat cgtgggtact actcctactg tggtgaggaa ctactacgct
     1681 aacacccaga agatcaagtc cgaggttaga ggtatcctgg gtgacgattt cggcagcaag
     1741 gacgtgttct tctctcactg gacctccaag tacaaagagc ggaaccctac cgagatcgct
     1801 tactctgagg atatcgagcg tatcatcgac agccttgtga ccgacgagat caccaaagaa
     1861 gagattatcc acttcctgtt cggcaacttc tgcttccaca tcgagactat gaacgaccag
     1921 cacattgccg acaagttcaa gggctatcag tcctcttgca tcaacctgaa gatcgagcct
     1981 aaggtggacc tggctgatct taaggatcac ctgattcaga agcagcagat ttgggagagc
     2041 ctgtacggaa agcacctcga gaagattatg ctgcggatcc gtgaaaagaa gaagaaagag
     2101 aaagagatcc cggacatcac caccgccttc aatcaaaatg ctgccgagta cgaagagaag
     2161 tacccgaact gcttcaccaa cgacctgtca gagacaaaga ccaacttctc tatgacctgg
     2221 tcaccgagct tcgagaaaat cgagctgtct agcgaggtgg actacaacaa tgccattatc
     2281 aacaagttcc gtgagagctt caagtccagc agcagagtga tctacaacag cccttacagc
     2341 tgcattaaca accagaccaa caaggcccgg gatatcacta accttgtgag gctttgcctg
     2401 accgagcttt cttgcgatac caccaagatg gaaaagcaag agcttgagga cgagatcgac
     2461 atcaacaccg gtagcattaa ggtcgagcgg accaagaaaa gcaaagagtg gaacaagcag
     2521 ggctcctgcc ttactaggaa caagaacgag ttctgcatga aggaaaccgg ccgagagaac
     2581 aagaccatct acttcaaggg tcttgccgtg atgaacatcg gcatgtcctc taagaagcgg
     2641 attctgaaga aagaggaaat caaagagagg atcagcaagg gcctcgagta cgatacttct
     2701 gagagacagg ctgatccgaa cgacgactac agcagcatcg atatgtcatc actgacccat
     2761 atgaagaagc tcatccggca cgataacgag gactcacttt cttggtgcga gcgaatcaag
     2821 gacagcttgt tcgtgcttca caacggcgat atcagagaag agggcaagat caccagcgtg
     2881 tacaacaatt acgctaagaa tcccgagtgc ctgtacatcc aggattctgt gcttaagacc
     2941 gagctggaaa cctgcaagaa aatcaacaaa ctgtgcaacg atctggccat ctaccactac
     3001 agcgaggaca tgatgcagtt ctccaagggt ttgatggtgg ccgatcggta catgaccaaa
     3061 gagtctttca agatcctgac caccgccaat accagcatga tgctgcttgc tttcaaaggc
     3121 gacggcatga acaccggcgg tagcggcgtg ccttacattg ccctgcacat cgtggacgag
     3181 gatatgtccg accagttcaa catctgctac accaaagaga tctacagcta cttccgcaac
     3241 ggctccaact acatctacat catgaggccg cagaggctca accaggtgag gcttctttca
     3301 ctgttcaaga caccttctaa ggtgccagtt tgcttcgccc agttcagcaa gaaggccaac
     3361 gagatggaaa agtggctgaa gaacaaggac attgagaagg tcaacgtgtt cagcatgacc
     3421 atgaccgtga agcagatcct gatcaacatc gtgttctcca gcgtgatgat tggcaccgtg
     3481 actaagcttt ctcggatggg catcttcgac ttcatgaggt acgctggttt cttgccgctg
     3541 tccgactact ccaacatcaa agagtacatc cgggacaagt tcgatccaga tattaccaat
     3601 gtggccgata tctacttcgt gaacgggatc aagaaactgc tgttccggat ggaagatttg
     3661 aacctgagca ccaacgcaaa gcctgttgtg gtggatcacg agaacgatat catcggtggc
     3721 atcaccgacc tgaacatcaa gtgccctatt actggttcta ccctgctgac cctcgaggac
     3781 ctgtacaaca acgtgtacct cgccatctac atgatgccga agtctctgca taaccacgtg
     3841 cacaacctta ccagcctgct taatgttcct gctgagtggg agctgaagtt ccggaaagag
     3901 cttggcttca acattttcga ggacatctac ccgaagaaag ccatgttcga tgacaaggac
     3961 ctcttcagca ttaacggcgc tcttaacgtg aaggccctga gcgattacta cctgggtaac
     4021 atcgagaatg tgggcctgat gaggtccgag attgagaaca aagaggactt cctgtctccg
     4081 tgctacaaga tctctaccct gaagtccagt aagaagtgca gccagagcaa catcatcagc
     4141 actgatgaga tcattgagtg cctgcagaac gcaaagattc aggacatcga aaactggaag
     4201 ggcaacaacc tggctattat caagggcctg atccggacct acaacgagga aaagaatcgg
     4261 ctggttgagt tcttcgagga taactgcgtg aacagcctgt acctggtcga gaagcttaaa
     4321 gagatcatta acagcggcag catcaccgtg ggaaagtctg tgactagcaa gttcatccgt
     4381 aacaatcacc cgctgaccgt ggaaacctac ctcaagacta agctgtacta tcggaacaac
     4441 gtgaccgtgc tgaagtctaa gaaggtgagc gaggaactgt acgacctcgt taagcagttc
     4501 cacaacatga tggaaatcga cctggactct gtgatgaacc ttggaaaggg tactgaggga
     4561 aagaagcaca ccttcttgca gatgcttgag ttcgtgatga gcaaggccaa gaatgtgacc
     4621 ggttctgtgg atttcctcgt gagcgtgttc gaaaagatgc agaggaccaa gaccgaccgt
     4681 gagatctacc tgatgagcat gaaggtgaag atgatgctgt acttcatcga gcataccttc
     4741 aagcacgtgg cccagtctga tccttctgag gctattagca tcagcggcga caacaagatt
     4801 agggctctgt ctacccttag cctggacacc attaccagct acaacgacat cctcaacaag
     4861 aatagcaaga agtctcggct ggctttcctg agcgctgatc aatctaagtg gtccgcttct
     4921 gacctgacct acaagtatgt gctggccatc attctgaacc cgattcttac tactggcgag
     4981 gcctctctta tgatcgagtg cattctgatg tatgtgaagc tgaagaaagt gtgcatcccg
     5041 accgacattt tcttgaacct taggaaggct cagggcacct tcggtcaaaa cgaaaccgct
     5101 attggtctgc tgaccaaggg acttaccacc aacacttacc cggtgtctat gaactggctg
     5161 cagggtaacc tgaactacct ctcttctgtg taccactcct gcgctatgaa ggcttaccac
     5221 aagactctcg agtgctataa ggactgcgac tttcagaccc ggtggatcgt tcactctgat
     5281 gataacgcaa ccagcctgat cgcttcaggt gaggttgaca agatgctgac cgacttctcc
     5341 tcttcatctc tgcctgagat gctgttcaga tccatcgagg ctcacttcaa gtctttctgc
     5401 atcacactca acccaaagaa gtcatacgcc agctcaagcg aggtcgagtt catttctgag
     5461 aggattgtga acggcgctat catcccactt tactgcagac atcttgctaa ctgctgcacc
     5521 gagtcctctc acatctccta cttcgatgac ctgatgtccc tgtctatcca cgtgaccatg
     5581 ttgctgagaa agggctgccc taatgaggtt atccctttcg cttacggtgc tgtgcaagtg
     5641 caggctctct ctatctactc tatgctgcct ggtgaggtga atgatagcat tcggatcttt
     5701 aagaagctgg gagtcagcct taagtctaat gagattccta ctaacatggg aggttggctg
     5761 acctctccaa ttgagccttt gtctattctg ggtccaagca gcaacgacca gatcatctac
     5821 tacaacgtca tccgcgattt cctgaacaag aaatcgctgg aggaggtgaa agactccgtg
     5881 tcgtcctcat cctacctgca gatgaggttc agggaactca agggcaagta cgagaagggt
     5941 actctggaag agaaggacaa gaaaatgatc ttccttatca acctcttcga gaaggccagc
     6001 gtgtccgagg atagtgatgt gcttaccatc gggatgaagt tccagaccat gctgacccag
     6061 atcatcaagc tgcccaactt catcaacgag aacgccttga acaagatgag cagctacaag
     6121 gacttctcca agctgtaccc taacctcaag aagaacgagg atctgtacaa gtccaccaag
     6181 aacctcaaaa tcgacgagga cgctatcctc gagggagatg agctttatga gaagatcgcc
     6241 agcagcctcg agatggaatc tgtgcacgac atcatgatca agaacccgga aaccattctg
     6301 atcgctccgc tgaacgatag ggactttctg ctgtctcagc tgttcatgta cacctctccg
     6361 tctaagagga accagctgtc taatcagtct accgagaagc tggctcttga tagggtgttg
     6421 agatctaagg ctaggacctt cgtggacatc tcctctaccg ttaagatgac ctatgaagag
     6481 aacatggaga agaagatctt ggagatgctc aagttcgatc tcgacagcta ctgcagcttc
     6541 aagacttgcg tgaacctcgt gatcaaggat gtgaacttct ccatgcttat cccgatcctc
     6601 gactctgctt atccttgcga gtctaggaag cgggacaact acaacttccg ttggttccag
     6661 actgagaagt ggatccctgt tgttgagggt tctcctggac ttgttgtgat gcatgctgtg
     6721 tacgggtcta actatatcga gaacctcggc cttaagaaca tcccgcttac cgacgactct
     6781 atcaacgtgc tgacttctac cttcggcacc ggtctgatta tggaagatgt caagagcctg
     6841 gtgaagggca aagactcatt tgagacagag gccttcagca actctaacga gtgccagaga
     6901 cttgtgaagg cctgcaacta catgatcgct gctcagaaca ggctcctggc tatcaatacc
     6961 tgctttaccc ggaagtcttt cccgttctac tccaagttca atcttggcag aggcttcatc
     7021 agcaacaccc ttgctttgct gtccaccatc tacagcaaag aggaatccta ccacttcgtg
     7081 tccaccgcct cttacaagct ggataagact atccggaccg tgatctctgc acagcaggat
     7141 atgaacctgg aaaagatcct ggataccgcc gtgtacatca gcgacaagct tcagtctttg
     7201 ttcccgacca tcaccagaga ggacatcgtt ctcattctcc agaacgtgtg cctggactcc
     7261 aagcctattt ggcagtcttt ggaggacaag atgaagaaga ttaacaacag caccgccagc
     7321 ggcttcaccg tgtctaatgt gattctgagc cacaactccg agctgaacac catccagaaa
     7381 cagatcgtgt ggatgtggaa catgggcctt tgctctcata ggaccctgga tttcgtgatc
     7441 cgttacatca ggcggtccga tgtgagatac gtcaagactg aggaacagga cgagagcggg
     7501 aactacatta gcggaaccat gtacaagatt ggcatcatga cccggtcttg ctacgtgcag
     7561 ttgatcgcat cagatcagga tgtggctgtg tctcttagga cacctttcga gattctcaac
     7621 gagagggatt acctgttcga cacctaccgt gagtccatcg aaaagctgct gcaaaagttc
     7681 atgttcgaca aggtcaacat tatcaagtct aagcagccgc agattgtgtt cctcgagcca
     7741 ggagatgctt gcattaggat gaccaccgac aacaaaatga tcgtcaaggt taacgccacc
     7801 agcaggcaga ttaggcttga gaacgttaag ctggtggtga agattaagta tgagaacgtg
     7861 aactccgacg tgtgggacat cattgagagc cagaagtctc tcgttctccg gcttcctgaa
     7921 gttggtgagt gcttcagcga catgtataag accgctgaca gcgagactga gacaatcaag
     7981 actatcaaga accggctgat gacctctctg accttcattg aggctttcgg caacctcagc
     8041 cagcagatca aagaaatcgt ggacgatgac atcagggaaa ccatggacga gttcctcatg
     8101 aacatccgtg atacctgcct tgagggtctt gagaattgca agagcgtgga agagtacgac
     8161 tcctaccttg atgagaacgg gttcaacgat accgtcgagc tgttcgagaa tcttctgagg
     8221 acccacgaca acttcgagaa cgagtactct ccactgttca gcgagatcgt ggataaggct
     8281 aagcagtaca ccagggatct cgagggcttt aaagaaatcc tgctgatgct gaagtactct
     8341 ctgatcaacg acgcctccgg gttcaagtca tatagagcta ctggtatgca cgccgttgag
     8401 ctgatggcta agaaacacat tgagatcggc gagttcaacc tgctgggtat gattcagctt
     8461 atcaaggctt gcgagacttg ccacaacaac gactccattc tcaacctggc cagccttagg
     8521 aatgtgctgt ctaggactta cgctactttc ggcaggcgga tcaggttgaa tcacgatctg
     8581 gatctgcaga acaaccttat ggaaaagagc tacgacttca agaccttggt gctccccgag
     8641 attaagctct ctgagctgag cagggaaatc ctgaaagaga acggcttcgt tatctccggc
     8701 gagaacctta agatggacag gtccgatgaa gagttcgagg gactcgcttc attcaatgtg
     8761 cttaggttgg acgaggaaga gatgtacgag ggactgatca aagagatgaa gatcaagcgg
     8821 aagaagaagg gctctctgtt ccctgctaat accctgcttc tgtccgagtt gatcaagttc
     8881 ctgatcggcg gtattaaggg caccagcttc gatattgaga ctctgctccg gaactcattc
     8941 aggccggata tcttctctac cgacaggctt ggtaggctct cttcttctgt gcctgctctt
     9001 aaggtgtacg ccactgtgta catggagtac aagaacgtca actgccctct gaacgagatc
     9061 gccgattctc ttgagggtta cctgaagctc accaagtcca agagcaaaga acacttcttg
     9121 agcggcaggg tgaagaaggc tcttattcag ttgagggacg agcagtccag gaccaagaaa
     9181 ctcgaggtct acaaggatat cgccaacttc cttagcaggc accctctttg cctgtctgaa
     9241 aagacactgt acggccggta cacctacagc gatatcaacg attacatcat gcagacccgg
     9301 gaaatcatcc tctctaagat ctccgagttg gatgaggtgg tggaaactga cgaggacaac
     9361 ttcctgctct cttaccttag aggcgaagag gatgctttcg atgaggacga ttctgatgag
     9421 gaagaggaca ccgattagat taaaagtaat gcctaacaat gcatgaataa tagattagac
     9481 agaacttaga atataaattt attgttattt agaattagat tagatctact tagcctaaag
     9541 acaatctggt gaaccaaatc tatagtgtat ataaatatag agtcccggta tagtttcact
     9601 ggagggaatt cttatataat ttatttgtaa agtctggctg tggagagatt atatgtttta
     9661 gttgtacctg attgctctgg gtcggcatgg catctccacc tcctcgcggt ccgacctggg
     9721 catccgaagg aggacgtcgt ccactcggat ggctaaggga gagctcgaat ttccccgatc
     9781 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     9841 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     9901 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     9961 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
    10021 tactagatcg gaattcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac
    10081 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
    10141 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacaggag
    10201 atctcagtaa agcgaaaagt gccacctgaa cgaagcatct gtgcttcatt ttgtagaaca
    10261 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
    10321 acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta
    10381 aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
    10441 acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt
    10501 ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact
    10561 ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc
    10621 cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga
    10681 ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa
    10741 ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg
    10801 atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc
    10861 tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca ctctatgaat
    10921 agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat aaaaaatgta
    10981 gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt tatataggga
    11041 tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg aagcggtatt
    11101 cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga aagtgcgtct
    11161 tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta gagaatagga
    11221 acttcggaat aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg
    11281 agctgcgcac atacagctca ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat
    11341 atatatacat gagaagaacg gcatagtgcg tgtttatgct taaatgcgta cttatatgcg
    11401 tctatttatg taggatgaaa ggtagtctag tacctcctgt gatattatcc cattccatgc
    11461 ggggtatcgt atgcttcctt cagcactacc ctttagctgt tctatatgct gccactcctc
    11521 aattggatta gtctcatcct tcaatgctat catttccttt gatattggat catactaaga
    11581 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    11641 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    11701 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    11761 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    11821 ccatagatca acgacattac tatatatata atataggaag catttaatag aacagcatcg
    11881 taatatatgt gtactttgca gttatgacgc cagatggcag tagtggaaga tattctttat
    11941 tgaaaaatag cttgtcacct tacgtacaat cttgatccgg agcttttctt tttttgccga
    12001 ttaagaatta attcggtcga aaaaagaaaa ggagagggcc aagagggagg gcattggtga
    12061 ctattgagca cgtgagtata cgtgattaag cacacaaagg cagcttggag tatgtctgtt
    12121 attaatttca caggtagttc tggtccattg gtgaaagttt gcggcttgca gagcacagag
    12181 gccgcagaat gtgctctaga ttccgatgct gacttgctgg gtattatatg tgtgcccaat
    12241 agaaagagaa caattgaccc ggttattgca aggaaaattt caagtcttgt aaaagcatat
    12301 aaaaatagtt caggcactcc gaaatacttg gttggcgtgt ttcgtaatca acctaaggag
    12361 gatgttttgg ctctggtcaa tgattacggc attgatatcg tccaactgca tggagatgag
    12421 tcgtggcaag aataccaaga gttcctcggt ttgccagtta ttaaaagact cgtatttcca
    12481 aaagactgca acatactact cagtgcagct tcacagaaac ctcattcgtt tattcccttg
    12541 tttgattcag aagcaggtgg gacaggtgaa cttttggatt ggaactcgat ttctgactgg
    12601 gttggaaggc aagagagccc cgaaagctta cattttatgt tagctggtgg actgacgcca
    12661 gaaaatgttg gtgatgcgct tagattaaat ggcgttattg gtgttgatgt aagcggaggt
    12721 gtggagacaa atggtgtaaa agactctaac aaaatagcaa atttcgtcaa aaatgctaag
    12781 aaataggtta ttactgagta gtatttattt aagtattgtt tgtgcacttg ccgatctatg
    12841 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg
    12901 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
    12961 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
    13021 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
    13081 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
    13141 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
    13201 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
    13261 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
    13321 ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg
    13381 gcgatcggtg cgggcctctt cgctattacg ccagatcctt gctggctgaa cccccagccg
    13441 gaactgaccc cacaaggccc tagcgtttgc aatgcaccag gtcatcattg acccaggcgt
    13501 gttccaccag gccgctgcct cgcaactctt cgcaggcttc gccgacctgc tcgcgccact
    13561 tcttcacgcg ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt
    13621 acggctcccg gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc gacagcttgc
    13681 ggtacttctc ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct
    13741 cgtcgatcag gacctggcaa cgggacgttt tcttgccacg gtccaggacg cggaagcggt
    13801 gcagcagcga caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg
    13861 cctgtaggcg cgacaggcat tcctcggcct tcgtgtaata ccggccattg atcgaccagc
    13921 ccaggtcctg gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct
    13981 tcgcgtactc caacacctgc tgccacacca gttcgtcatc gtcggcccgc agctcgacgc
    14041 cggtgtaggt gatcttcacg tccttgttga cgtggaaaat gaccttgttt tgcagcgcct
    14101 cgcgcgggat tttcttgttg cgcgtggtga acagggcaga gcgggccgtg tcgtttggca
    14161 tcgctcgcat cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc atttccttga
    14221 tctgctgctt cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca
    14281 ggtcctcgcc ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca
    14341 tcgacttcgc caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc acggcggccg
    14401 atggcgcggg cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag
    14461 cttgctgggc catcgagccg acggactgga aggtttcgcg gggcgcacgc atgacggtgc
    14521 ggcttgcgat ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt
    14581 atgccttccg gtcaaacgtc cgattcattc accctccttg cgggattgcc ccgactcacg
    14641 ccggggcaat gtgcccttat tcctgatttg acccgcctgg tgccttggtg tccagataat
    14701 ccaccttatc ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg
    14761 tattccgaat cttgccctgc acgaatacca gcgacccctt gcccaaatac ttgccgtggg
    14821 cctcggcctg agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc
    14881 cggcatcgtt gcgccacatc taggtactaa aacaattcat ccagtaaaat ataatatttt
    14941 attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct
    15001 tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc
    15061 ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg
    15121 ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa
    15181 aaatcataca gctcgcgcgg atctttaaat ggggtgtctt cttcccagtt ttcgcaatcc
    15241 acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta
    15301 ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac
    15361 agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca
    15421 tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaggtg caggaccttt
    15481 ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag
    15541 gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc
    15601 agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg
    15661 atcagttttt tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt
    15721 ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac
    15781 tgttccttgc attctaaaac tttaaatacc agaaaacagc tttttcaaag ttgttttcaa
    15841 agttggcgta taacatagta tcgacggagc cgattttgaa accacaatta tgggtgatgc
    15901 tgcaactcga gcgggccggg agggttcgag aagggggggc accccccttc ggcgtgcgcg
    15961 gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt tataaatatt ggtttaaaag
    16021 caggttaaaa gacaggttag cggtggccga aaaacgggcg gaaacccttg caaatgctgg
    16081 attttctgcc tgtggacagc ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac
    16141 tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt
    16201 gtcaataccg cagggcactt atccccaggc ttgtccacat catctgtggg aaactcgcgt
    16261 aaaatcaggc gttttcgccg atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc
    16321 gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt
    16381 ccgcccctca tctgtcagtg agggccaagt tttccgcgag gtatccacaa cgccggcggc
    16441 cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg
    16501 ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt ctctagtgga ctgatgggct
    16561 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
    16621 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
    16681 gacgttttta atgtactggg gtggtttt
//