| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RAI1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001415 |
| 产品用途: | 3' AAVLINK plasmid for RAI1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
RAI1
|
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RAI1-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RAI1-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RAI1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RAI1-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RAI1-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RAI1-FLAG 6987 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6987)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6987
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3700
/codon_start=1
/label=RAI1
/translation="VSSSPQKEGRVSQRARVPKPGAGSKLSDRPLHALKRKSAFMAPVP
TKKRNLVLRSRSSSSSNASGNGGDGKEERPEGSPTLFKRMSSPKKAKPTKGNGEPATKL
PPPETPDACLKLASRAAFQGAMKTKVLPPRKGRGLKLEAIVQKITSPSLKKFACKAPGA
SPGNPLSPSLSDKDRGLKGAGGSPVGVEEGLVNVGTGQKLPTSGADPLCRNPTNRSLKG
KLMNSKKLSSTDCFKTEAFTSPEALQPGGTALAPKKRSRKGRAGAHGLSKGPLEKRPYL
GPALLLTPRDRASGTQGASEDNSGGGGKKPKMEELGLASQPPEGRPCQPQTRAQKQPGH
TNYSSYSKRKRLTRGRAKNTTSSPCKGRAKRRRQQQVLPLDPAEPEIRLKYISSCKRLR
SDSRTPAFSPFVRVEKRDAFTTICTVVNSPGDAPKPHRKPSSSASSSSSSSSFSLDAAG
ASLATLPGGSILQPRPSLPLSSTMHLGPVVSKALSTSCLVCCLCQNPANFKDLGDLCGP
YYPEHCLPKKKPKLKEKVRPEGTCEEASLPLERTLKGPECAAAATAGKPPRPDGPADPA
KQGPLRTSARGLSRRLQSCYCCDGREDGGEEAAPADKGRKHECSKEAPAEPGGEAQEHW
VHEACAVWTGGVYLVAGKLFGLQEAMKVAVDMMCSSCQEAGATIGCCHKGCLHTYHYPC
ASDAGCIFIEENFSLKCPKHKRLP"
misc_feature 1566..1571
/label=SDS
misc_feature 1758..1763
/label=SDS
misc_feature 2464..2469
/label=SDS
misc_feature 2865..2870
/label=SDS
misc_feature 2874..2879
/label=SDS
misc_feature 2967..2972
/label=SDS
misc_feature 3171..3176
/label=SDS
misc_feature 3363..3368
/label=SDS
misc_feature 3408..3413
/label=SDS
misc_feature 3519..3524
/label=SDS
CDS 3707..3772
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3776..4207
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3920..3925
/label=SDS
repeat_region 4250..4390
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4250..4379
/label=AAV2 ITR
rep_origin 4465..4920
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5202..5306
/gene="bla"
/label=AmpR promoter
CDS 5307..6167
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6338..6926
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtct ccagcagccc
1561 ccagaaggag ggcagggtga gccagcgggc aagggtcccc aaacctggtg caggcagcaa
1621 gctctctgac cggcccctcc atgcgctcaa aaggaagtcg gccttcatgg cgccggtccc
1681 caccaagaag cggaacctgg tcttgcggag ccgcagcagc agcagcagca acgccagtgg
1741 caatggggga gatgggaagg aggagaggcc tgagggttcc cccaccctct tcaagaggat
1801 gtcttctccc aagaaagcca agcccaccaa gggcaatggc gagcctgcca caaagctccc
1861 acccccggag acccccgatg cctgcctcaa gctcgcctct cgggcagcct tccagggggc
1921 catgaagacc aaggtgctgc caccccggaa gggccggggc ctgaagctgg aagccatcgt
1981 gcagaagatc acctcgccca gcctcaagaa gttcgcatgt aaagcgccag gggcctctcc
2041 tggtaatcct ctgagcccat ccctttccga caaagaccgt gggctcaagg gtgctggggg
2101 cagcccagtg ggggtggaag aaggcctggt aaatgtgggc accgggcaga agctcccaac
2161 ttctggggct gatccgttat gcagaaatcc aaccaacaga tccttaaaag gcaaactcat
2221 gaacagtaag aaactgtctt ctactgactg tttcaaaacc gaggccttca catccccgga
2281 ggccctgcag cctgggggga ctgccctggc gcctaagaag aggagccgga aaggccgggc
2341 aggggcccat ggactctcca aaggcccgct ggagaagcgg ccctatcttg gcccggctct
2401 gctcctgact ccccgagaca gggccagtgg cacacaaggg gccagtgagg acaactctgg
2461 tggaggaggc aagaagccaa agatggagga gctgggcctg gcctcccagc ccccggaggg
2521 caggccctgc cagccccaga caagggcaca gaaacagcca ggccacacca actacagcag
2581 ctattccaag cggaagcgcc tcactcgggg ccgggccaag aacaccacct cttcaccctg
2641 taaggggcgt gccaagcgac gacgacagca gcaggtgctg cccctggatc ccgcagagcc
2701 tgaaatccgc ctcaagtaca tttcctcttg caagcggctg aggtcagaca gccggacccc
2761 cgccttctca cccttcgtgc gggtggagaa gcgagacgcg ttcaccacca tatgcactgt
2821 tgtcaactcc cctggagatg cgcccaagcc ccacaggaag ccttcctcct ctgcctcctc
2881 ttcctcatcc tcgtcctcgt tctccttgga tgcagccggg gcctccctgg ccacactccc
2941 tggaggctcc atcctgcagc cgcggccctc cttgcccctc tcctccacga tgcacttggg
3001 gcctgtggtt tccaaggccc tgagtacctc ttgccttgtt tgctgcctct gccaaaaccc
3061 ggccaacttc aaggaccttg gggacctctg tgggccctac taccctgaac actgcctccc
3121 caaaaagaag ccaaaactca aggagaaggt gcggccagaa ggcacctgtg aggaggcctc
3181 gctgccgctt gagagaacac tcaaaggtcc cgagtgtgca gctgccgcca ctgccgggaa
3241 gccccccagg cctgacggcc cagctgaccc ggccaagcag ggcccactgc gcaccagtgc
3301 ccggggcctg tcccggaggc tgcagagctg ctactgctgt gatggccggg aggatggggg
3361 cgaggaggca gccccagccg acaagggtcg caaacatgag tgcagcaagg aggctccggc
3421 agagcccggc ggggaggccc aggagcactg ggtgcatgag gcctgtgccg tgtggaccgg
3481 cggcgtctac ctggtggccg ggaagctctt tgggctgcag gaggccatga aggtggccgt
3541 ggacatgatg tgttccagct gccaagaagc cggggccacc atcgggtgct gccacaaagg
3601 atgcctccac acctaccact acccgtgtgc cagcgatgca ggttgcatat tcatcgaaga
3661 gaacttttct ttgaaatgtc ccaaacataa gaggctgccg tgtacagact acaaagacca
3721 tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca agtaactcga
3781 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
3841 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
3901 cgtatggctt tcattttctc ctccttgtat aaatcctggt tagttcttgc cacggcggaa
3961 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
4021 tccgtggtgt ttatttgtga aatttgtgat gctattgctt tatttgtaac catctagctt
4081 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
4141 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
4201 tttttaaaga tctggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta
4261 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
4321 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
4381 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
4441 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
4501 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg
4561 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
4621 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
4681 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
4741 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
4801 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
4861 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
4921 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
4981 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
5041 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
5101 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
5161 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
5221 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
5281 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
5341 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
5401 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
5461 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
5521 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
5581 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
5641 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
5701 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
5761 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
5821 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
5881 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
5941 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
6001 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
6061 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
6121 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
6181 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
6241 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
6301 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
6361 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
6421 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
6481 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
6541 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
6601 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
6661 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
6721 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
6781 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
6841 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
6901 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
6961 ccttttgctg gccttttgct cacatgt
//
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