| 质粒名称: | GAL4UAS-Luciferase reporter |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 64125 |
| 存储实验室: | Moritoshi Sato |
|
载体骨架基本信息 |
|
| 载体名称: | pGL4.31 |
| 载体出品公司: | Promega |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3500 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，荧光素酶 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GAL4UAS |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | GAL4UAS |
| 标签/融合蛋白: | luciferase（主干上的C末端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | HindIII(未破坏) |
| 3' 克隆位点: | XbaI (未破坏) |
| 5' 测序引物 | LucNRev |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | GAL4UAS-Luciferase reporter was a gift from Moritoshi Sato (Addgene plasmid # 64125 ; http://n2t.net/addgene:64125 ; RRID:Addgene_64125) |
| 参考文献部分: | CRISPR-Cas9-based Photoactivatable Transcription System. Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22. 10.1016/j.chembiol.2014.12.011 PubMed 25619936 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS GAL4UAS-Luciferase reporter 5966 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Photoactivatable transcription system. Luciferase reporter under the
control of the upstream activator sequence (UAS) of Gal4..
ACCESSION .
VERSION .
KEYWORDS GAL4UAS-Luciferase reporter
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5966)
AUTHORS Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
TITLE CRISPR-Cas9-based Photoactivatable Transcription System.
JOURNAL Chem Biol. 2015 Feb 19;22(2):169-74. doi:
10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
PUBMED 25619936
REFERENCE 2 (bases 1 to 5966)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5966
/organism="synthetic DNA construct"
/mol_type="other DNA"
regulatory 232..241
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
polyA_signal 1921..2042
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1932..1951)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 1986..2005
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
promoter 2236..2593
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2259..2279)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2444..2579
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2506..2525
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2624..3661
/codon_start=1
/product="hygromycin B phosphotransferase"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
EVGRV"
RBS 3640..3648
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
polyA_signal 3685..3733
/note="synthetic polyadenylation signal"
primer_bind complement(3889..3906)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4060..4648)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4140..4159)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4848..5708)
/codon_start=1
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 5813..5861
/note="synthetic polyadenylation signal"
misc_feature 5875..5966
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 5915..5934
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 ggcctaactg gccggtaccg agtttctaga cggagtactg tcctccgagc ggagtactgt
61 cctccgactc gagcggagta ctgtcctccg atcggagtac tgtcctccgc gaattccgga
121 gtactgtcct ccgaagacgc tagcgggggg ctataaaagg gggtgggggc gttcgtcctc
181 actctagatc tgcgatctaa gtaagcttgg caatccggta ctgttggtaa agccaccatg
241 gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga agacgggacc
301 gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg caccatcgcc
361 tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga gatgagcgtt
421 cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat cgtggtgtgc
481 agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat cggtgtggct
541 gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat gggcatcagc
601 cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag
661 aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta ccagggcttc
721 caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga gtacgacttc
781 gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag tagtggcagt
841 accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg attcagtcat
901 gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct cagcgtggtg
961 ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg
1021 gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca agactataag
1081 attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc
1141 gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag
1201 gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc
1261 ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca
1321 gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca
1381 ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac
1441 gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct gcacagcggc
1501 gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg
1561 atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac
1621 cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc
1681 gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat
1741 gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag
1801 gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat tctcattaag
1861 gccaagaagt aaggccgcga ctctagagtc ggggcggccg gccgcttcga gcagacatga
1921 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
1981 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
2041 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
2101 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tttgcgtatt
2161 gggcgctctt ccgctgatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac
2221 ttggttagct accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
2281 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
2341 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
2401 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
2461 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
2521 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
2581 aggcttttgc aaaaagctcg attcttctga cactagcgcc accatgaaga agcccgaact
2641 caccgctacc agcgttgaaa aatttctcat cgagaagttc gacagtgtga gcgacctgat
2701 gcagttgtcg gagggcgaag agagccgagc cttcagcttc gatgtcggcg gacgcggcta
2761 tgtactgcgg gtgaatagct gcgctgatgg cttctacaaa gaccgctacg tgtaccgcca
2821 cttcgccagc gctgcactac ccatccccga agtgttggac atcggcgagt tcagcgagag
2881 cctgacatac tgcatcagta gacgcgccca aggcgttact ctccaagacc tccccgaaac
2941 agagctgcct gctgtgttac agcctgtcgc cgaagctatg gatgctattg ccgccgccga
3001 cctcagtcaa accagcggct tcggcccatt cgggccccaa ggcatcggcc agtacacaac
3061 ctggcgggat ttcatttgcg ccattgctga tccccatgtc taccactggc agaccgtgat
3121 ggacgacacc gtgtccgcca gcgtagctca agccctggac gaactgatgc tgtgggccga
3181 agactgtccc gaggtgcgcc acctcgtcca tgccgacttc ggcagcaaca acgtcctgac
3241 cgacaacggc cgcatcaccg ccgtaatcga ctggtccgaa gctatgttcg gggacagtca
3301 gtacgaggtg gccaacatct tcttctggcg gccctggctg gcttgcatgg agcagcagac
3361 tcgctacttc gagcgccggc atcccgagct ggccggcagc cctcgtctgc gagcctacat
3421 gctgcgcatc ggcctggatc agctctacca gagcctcgtg gacggcaact tcgacgatgc
3481 tgcctgggct caaggccgct gcgatgccat cgtccgcagc ggggccggca ccgtcggtcg
3541 cacacaaatc gctcgccgga gcgcagccgt atggaccgac ggctgcgtcg aggtgctggc
3601 cgacagcggc aaccgccggc ccagtacacg accgcgcgct aaggaggtag gtcgagttta
3661 aactctagaa ccggtcatgg ccgcaataaa atatctttat tttcattaca tctgtgtgtt
3721 ggttttttgt gtgttcgaac tagatgctgt cgaccgatgc ccttgagagc cttcaaccca
3781 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
3841 tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga
3901 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
3961 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
4021 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
4081 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
4141 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
4201 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
4261 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
4321 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
4381 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
4441 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
4501 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
4561 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
4621 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
4681 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
4741 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
4801 gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact gcagtggtta ccagtgcttg
4861 atcagtgagg caccgatctc agcgatctgc ctatttcgtt cgtccatagt ggcctgactc
4921 cccgtcgtgt agatcactac gattcgtgag ggcttaccat caggccccag cgcagcaatg
4981 atgccgcgag agccgcgttc accggccccc gatttgtcag caatgaacca gccagcaggg
5041 agggccgagc gaagaagtgg tcctgctact ttgtccgcct ccatccagtc tatgagctgc
5101 tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt tccgaagagt tgtggccatt
5161 gctactggca tcgtggtatc acgctcgtcg ttcggtatgg cttcgttcaa ctctggttcc
5221 cagcggtcaa gccgggtcac atgatcaccc atattatgaa gaaatgcagt cagctcctta
5281 gggcctccga tcgttgtcag aagtaagttg gccgcggtgt tgtcgctcat ggtaatggca
5341 gcactacaca attctcttac cgtcatgcca tccgtaagat gcttttccgt gaccggcgag
5401 tactcaacca agtcgttttg tgagtagtgt atacggcgac caagctgctc ttgcccggcg
5461 tctatacggg acaacaccgc gccacatagc agtactttga aagtgctcat catcgggaat
5521 cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat tgagatccag ttcgatatag
5581 cccactcttg cacccagttg atcttcagca tcttttactt tcaccagcgt ttcggggtgt
5641 gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg
5701 atgctcatac tcgtcctttt tcaatattat tgaagcattt atcagggtta ctagtacgtc
5761 tctcaaggat aagtaagtaa tattaaggta cgggaggtat tggacaggcc gcaataaaat
5821 atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata
5881 cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc
5941 aagtgcaggt gccagaacat ttctct
//
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