pLVX-Hom-1

价格:3500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-Hom-1
质粒类型: 慢病毒载体;双顺反子载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 8478 bp
5' 测序引物及序列: CMV Forward: CGCAAATGGGCGGTAGGCGTG 
3' 测序引物及序列: --
载体标签: DmrB (C、N-端均可)
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 慢病毒载体pLVX-Hom-1组成型表达DmrB融合蛋白;
外源基因与嘌呤霉素抗性基因以双顺反子的形式表达。
产品目录号: 635073
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-Hom-1载体图谱



pLVX-Hom-1 多克隆位点

pLVX-Hom-1 载体特征

载体简介

载体序列

LOCUS       pLVX-Hom-1   8478 bp    DNA   circular             5-OCT-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Catalog Nos. 635072, 635073      
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    2028..2151
                     /label=cPPT/CTS
     misc_feature    4400..4991
                     /label=WPRE
     rep_origin      complement(6301..6971)
                     /label=pUC\ori
     CDS             complement(7116..8112)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             5195..5831
                     /label=3'LTR
     misc_feature    3179..3754
                     /label=IRES
     CDS             3787..4386
                     /label=Puro(R)
     misc_feature    2821..3153
                     /label=DmrB
     misc_feature    2185..2787
                     /label=pCMV
BASE COUNT     2169 a      2111 c      2190 g      2008 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt 
     2821 atggcttcta gaggagtgca ggtggagact atctccccag gagacgggcg caccttcccc 
     2881 aagcgcggcc agacctgcgt ggtgcactac accgggatgc ttgaagatgg aaagaaagtt 
     2941 gattcctccc gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc 
     3001 cgaggctggg aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata 
     3061 tctccagatt atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact 
     3121 ctcgtcttcg atgtggagct tctaaaactg gaaagcggcc gcggatccta cgtatgaccg 
     3181 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg 
     3241 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg 
     3301 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg 
     3361 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca 
     3421 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct 
     3481 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca 
     3541 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa 
     3601 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg 
     3661 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg 
     3721 ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaacccac aaggagacga 
     3781 ccttccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg 
     3841 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac 
     3901 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 
     3961 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 
     4021 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 
     4081 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 
     4141 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 
     4201 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 
     4261 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 
     4321 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 
     4381 gcctagacgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg 
     4441 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 
     4501 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 
     4561 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 
     4621 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 
     4681 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 
     4741 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 
     4801 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 
     4861 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 
     4921 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 
     4981 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt 
     5041 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag 
     5101 gtgggttttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt 
     5161 agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg 
     5221 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca 
     5281 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct 
     5341 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt 
     5401 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt 
     5461 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa 
     5521 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg 
     5581 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg 
     5641 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 
     5701 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 
     5761 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 
     5821 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat 
     5881 gaatatcaga gagtgagagg ccttgacatt gctagcgttt taccgtcgac ctctagctag 
     5941 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 
     6001 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 
     6061 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 
     6121 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 
     6181 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 
     6241 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 
     6301 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 
     6361 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 
     6421 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 
     6481 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 
     6541 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 
     6601 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 
     6661 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 
     6721 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 
     6781 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 
     6841 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt 
     6901 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 
     6961 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 
     7021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 
     7081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 
     7141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 
     7201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 
     7261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 
     7321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 
     7381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 
     7441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 
     7501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 
     7561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 
     7621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 
     7681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 
     7741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 
     7801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 
     7861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 
     7921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 
     7981 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 
     8041 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 
     8101 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa 
     8161 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 
     8221 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc 
     8281 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctagt 
     8341 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta 
     8401 tttgttaaat atgtactaca aacttagtag tttttaaaga aattgtattt gttaaatatg 
     8461 tactacaaac ttagtagt 
//

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