| 载体名称: | pQE-22 |
|---|---|
| 质粒类型: | 大肠杆菌载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | -- |
| 启动子: | T5 |
| 载体大小: | 3998 bp |
| 5' 测序引物及序列: | pQE30-F: AGCGGATAACAATTTCACACAG |
| 3' 测序引物及序列: | pQE30-R: TTCTGAGGTCATTACTGGATC |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | M15 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pQE22载体基本信息
pQE22载体图谱质粒图谱和多克隆位点信息
pQE22载体简介
pQE22载体序列
LOCUS pQE-22 3998 bp ds-DNA circular SYN 05-DEC-2013
DEFINITION Bacterial vector for expressing DHFR fusion proteins with a
C-terminal 6xHis tag. Similar to pQE-16 and pQE-19, but with a
different reading frame after the DHFR.
ACCESSION .
VERSION .
KEYWORDS pQE-22
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3998)
AUTHORS Qiagen
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
COMMENT Discontinued pQE vector.
FEATURES Location/Qualifiers
source 1..3998
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 10..54
/note="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 30..46
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 62..78
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 101..106
/note="ribosome binding site"
CDS 115..117
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 133..690
/codon_start=1
/gene="Mus musculus Dhfr"
/product="mouse dihydrofolate reductase"
/note="DHFR"
/translation="MVRPLNSIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
PGVLSEVQEEKGIKYKFEVYEKK"
CDS 704..721
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
misc_feature 728..738
/note="stop codons"
/note="stop codons in all three reading frames"
terminator 744..838
/note="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS 882..1541
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
terminator 1606..1692
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin complement(2174..2762)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2933..3793)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQAAMDERNRQIAEIGAS
LIKHW"
promoter complement(3794..3898)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
121 ggatccggca tcatggttcg accattgaac tcgatcgtcg ccgtgtccca aaatatgggg
181 attggcaaga acggagacct accctggcct ccgctcagga acgagttcaa gtacttccaa
241 agaatgacca caacctcttc agtggaaggt aaacagaatc tggtgattat gggtaggaaa
301 acctggttct ccattcctga gaagaatcga cctttaaagg acagaattaa tatagttctc
361 agtagagaac tcaaagaacc accacgagga gctcattttc ttgccaaaag tttggatgat
421 gccttaagac ttattgaaca accggaattg gcaagtaaag tagacatggt ttggatagtc
481 ggaggcagtt ctgtttacca ggaagccatg aatcaaccag gccaccttag actctttgtg
541 acaaggatca tgcaggaatt tgaaagtgac acgtttttcc cagaaattga tttggggaaa
601 tataaacttc tcccagaata cccaggcgtc ctctctgagg tccaggagga aaaaggcatc
661 aagtataagt ttgaagtcta cgagaagaaa ggttggaaga tctcatcacc atcaccatca
721 ctaagcttaa ttagctgagc ttggactcct gttgatagat ccagtaatga cctcagaact
781 ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc
841 aagctagctt ggcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
901 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
961 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
1021 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
1081 tgaatgctca tccggaattt cgtatggcaa tgaaagacgg tgagctggtg atatgggata
1141 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
1201 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
1261 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
1321 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
1381 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
1441 tggcgattca ggttcatcat gccgtctgtg atggcttcca tgtcggcaga atgcttaatg
1501 aattacaaca gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
1561 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
1621 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
1681 agtaggacaa atccgccgct ctagagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
1741 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
1801 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
1861 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
1921 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
1981 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc tgtcggctgc
2041 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
2101 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
2161 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
2221 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
2281 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
2341 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
2401 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
2461 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
2521 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
2581 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
2641 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
2701 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
2761 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
2821 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
2881 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
2941 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagctgcct
3001 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
3061 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
3121 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
3181 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
3241 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
3301 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
3361 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
3421 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
3481 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
3541 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
3601 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
3661 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
3721 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
3781 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
3841 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
3901 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
3961 ataaaaatag gcgtatcacg aggccctttc gtcttcac
//
