| 质粒名称: | pMOD_C2515 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 91083 |
| 存储实验室: | Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pMOD_C0000 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning) |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | AtU6 |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMOD_C2515 was a gift from Daniel Voytas (Addgene plasmid # 91083 ; http://n2t.net/addgene:91083 ; RRID:Addgene_91083) |
| 参考文献部分: | A multi-purpose toolkit to enable advanced genome engineering in plants. Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. 10.1105/tpc.16.00922 PubMed 28522548 |
买家导航
pMODC2515载体基本信息
pMODC2515载体图谱质粒图谱和多克隆位点信息
pMODC2515载体简介
pMODC2515载体序列
LOCUS pMOD_C2515 3267 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Module C, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA
spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol
III.
ACCESSION .
VERSION .
KEYWORDS pMOD_C2515
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3267)
AUTHORS Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E,
Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
TITLE A multi-purpose toolkit to enable advanced genome engineering in
plants.
JOURNAL Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi:
10.1105/tpc.16.00922.
PUBMED 28522548
REFERENCE 2 (bases 1 to 3267)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3267
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 80..668
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 569..588
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 822..839
/label=L4440
/note="L4440 vector, forward primer"
CDS complement(1331..1636)
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind complement(1433..1452)
/label=ccdB-fwd
/note="ccdB gene, forward primer"
misc_RNA 1963..2038
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 2188..2279
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
841 aagcggaaga gcgcccacct gcaacgccgg cgccatggct tcgttgaaca acggaaactc
901 gacttgcctt ccgcacaata catcatttct tcttagcttt ttttcttctt cttcgttcat
961 acagtttttt tttgtttatc agcttacatt ttcttgaacc gtagctttcg ttttcttctt
1021 tttaactttc cattcggagt ttttgtatct tgtttcatag tttgtcccag gattagaatg
1081 attaggcatc gaaccttcaa gaatttgatt gaataaaaca tcttcattct taagatatga
1141 agataatctt caaaaggccc ctgggaatct gaaagaagag aagcaggccc atttatatgg
1201 gaaagaacaa tagtatttct tatataggcc catttaagtt gaaaacaatc ttcaaaagtc
1261 ccacatcgct tagataagaa aacgaagctg agtttatata cagctagagt cgaagtagtg
1321 attggagacg ttatattccc cagaacatca ggttaatggc gtttttgatg tcattttcgc
1381 ggtggctgag atcagccact tcttccccga taacggaaac cggcacactg gccatatcgg
1441 tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacgggaga
1501 ctttatctga cagcagacgt gcactggcca gggggatcac catccgtcgc ccgggcgtgt
1561 caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt
1621 aaaccttaaa ctgcatttca ccagcccctg ttctcgtcag caaaagagcc gttcatttca
1681 ataaaccggg cgacctcagc catcccttcc tgattttccg ctttccagcg ttcggcacgc
1741 agacgacggg cttcattctg catggttgtg cttaccagac cggagatatt gacatcatat
1801 atgccttgag caactgatag ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag
1861 catacctcct tttgacatac ttcgggtata catatcagta tatattctta taccgcaaaa
1921 atcagcgcgc aaatacgcat actgttatct ggcttcgtct ctgttttaga gctagaaata
1981 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
2041 tttttcgcgc gtagtcctcg gtaatatcgc agaacaaaag tacctgatat cgagtgtact
2101 tcaagtcaca ccggcgagtg actagcaggt gatgaaaagc cttagggtca ggtggcactt
2161 ttcggggaaa tgtgcgatga acccctattt gtttattttt ctaaatacat tcaaatatgt
2221 atccgctaat gagacaataa ccctgataaa tgcttcaatt atattgaaaa aggaagagta
2281 tgagtatcca acatttccgt gtcgccctta ttcccttttt tgcggccttc tgccttcctg
2341 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgccc
2401 gagtgggtta cattgaactg gatctcaaca gcggcaaaat cttagagagt tttcgccccg
2461 aagaacgctt cccaatgatg agcactttca aagttctgct atgtggcgcg gtgttatccc
2521 gtattgatgc tgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
2581 ttgagtattc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
2641 gtagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgattg
2701 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
2761 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
2821 ctgtagcaat ggcaacaact ctccgcaaac tattaactgg cgaactactt actcttgctt
2881 cacgccagca actcattgac tggatggagg cggacaaagt tgcaggacca cttctgcgct
2941 cggcacttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
3001 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
3061 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
3121 cactgattaa gcattggtaa ctgtcagacg aagggaaata aatagtagcc cgcctgatgt
3181 gcgggctttt ttttggtggt ggttaccagt gtcgcttagc taccgtaggt cctcttaggc
3241 ggtccgatta gcgacatagt catcaat
//
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pMODC2515载体质粒应用举例
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