| 质粒名称: | pTC362 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 91216 |
| 存储实验室: | Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pTRANS_100 |
| 载体出品公司: | -- |
| 载体抗性: | 大观霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达,CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | gRNAs targeting 6 tomato genes |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pTC362 was a gift from Daniel Voytas (Addgene plasmid # 91216 ; http://n2t.net/addgene:91216 ; RRID:Addgene_91216) |
| 参考文献部分: | A multi-purpose toolkit to enable advanced genome engineering in plants. Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. 10.1105/tpc.16.00922 PubMed 28522548 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pTC362 10048 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION protoplast vector for targeted deletion of 6 genes in tomato.
ACCESSION .
VERSION .
KEYWORDS pTC362
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10048)
AUTHORS Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E,
Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
TITLE A multi-purpose toolkit to enable advanced genome engineering in
plants.
JOURNAL Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi:
10.1105/tpc.16.00922.
PUBMED 28522548
REFERENCE 2 (bases 1 to 10048)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10048
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 84..672
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 573..592
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 826..843
/label=L4440
/note="L4440 vector, forward primer"
promoter 1389..1734
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind 1677..1697
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
CDS 2326..2382
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 2383..6486
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 6499..6519
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
protein_bind 7280..7294
/label=Csy4 site
/bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
/note="cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)"
misc_RNA 7315..7390
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
protein_bind 7396..7410
/label=Csy4 site
/bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
/note="cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)"
misc_RNA 7431..7506
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
protein_bind 7512..7526
/label=Csy4 site
/bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
/note="cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)"
misc_RNA 7547..7622
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
protein_bind 7628..7642
/label=Csy4 site
/bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
/note="cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)"
misc_RNA 7663..7738
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
protein_bind 7744..7758
/label=Csy4 site
/bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
/note="cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)"
misc_RNA 7779..7854
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
protein_bind 7860..7874
/label=Csy4 site
/bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
/note="cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)"
misc_RNA 7895..7970
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
protein_bind 7976..7990
/label=Csy4 site
/bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
/note="cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)"
misc_RNA 8011..8086
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
protein_bind 8092..8106
/label=Csy4 site
/bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
/note="cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)"
misc_RNA 8127..8202
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
protein_bind 8208..8222
/label=Csy4 site
/bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
/note="cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)"
misc_RNA 8243..8318
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
protein_bind 8324..8338
/label=Csy4 site
/bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
/note="cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)"
misc_RNA 8359..8434
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
protein_bind 8440..8454
/label=Csy4 site
/bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
/note="cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)"
misc_RNA 8475..8550
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
protein_bind 8556..8570
/label=Csy4 site
/bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
/note="cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)"
misc_RNA 8591..8666
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
protein_bind 8672..8686
/label=Csy4 site
/bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
/note="cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)"
polyA_signal 8706..8880
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS 9248..10039
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
1 cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
61 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
721 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
781 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
841 gaggaagcgg gagagcgccc atatgcgcac tcctcgcatg cggcgcgccg atcggcgcgc
901 cagatttgcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
961 gcagcaggta tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
1021 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
1081 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
1141 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
1201 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
1261 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
1321 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
1381 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
1441 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
1501 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
1561 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
1621 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
1681 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
1741 actcctgcag gaaaaatgga tcattatctt gatattagac ttagacctga tccagaattt
1801 ccaccagctc aacttatgtc tgttcttttt ggaaaacttc atcaagctct tgttgctcaa
1861 ggaggagata gaattggagt ttcttttcct gatcttgatg aatcaagatc aagacttgga
1921 gaaagactta gaattcatgc ttctgctgat gatcttagag ctttgcttgc tagaccttgg
1981 cttgaaggac ttagagatca tcttcaattt ggagaaccag ctgttgttcc acatccaact
2041 ccttatagac aagtttcaag agttcaagct aaatctaatc cagaaagact tagaagaaga
2101 cttatgagaa gacatgatct ttctgaagaa gaagctagaa aaagaattcc tgatactgtt
2161 gctagagctt tggatttgcc ttttgttaca cttagatcac aatctactgg acaacatttt
2221 agacttttta ttagacatgg accacttcaa gttactgctg aagaaggagg atttacttgt
2281 tatggacttt ctaagggagg ttttgttcct tggtttggat ctggagctac taatttttct
2341 cttcttaagc aagctggaga tgttgaagaa aatcctggac ccatggataa gaagtactct
2401 atcggactcg atatcggaac taactctgtg ggatgggctg tgatcaccga tgagtacaag
2461 gtgccatcta agaagttcaa ggttctcgga aacaccgata ggcactctat caagaaaaac
2521 cttatcggtg ctctcctctt cgattctggt gaaactgctg aggctaccag actcaagaga
2581 accgctagaa gaaggtacac cagaagaaag aacaggatct gctacctcca agagatcttc
2641 tctaacgaga tggctaaagt ggatgattca ttcttccaca ggctcgaaga gtcattcctc
2701 gtggaagaag ataagaagca cgagaggcac cctatcttcg gaaacatcgt tgatgaggtg
2761 gcataccacg agaagtaccc tactatctac cacctcagaa agaagctcgt tgattctact
2821 gataaggctg atctcaggct catctacctc gctctcgctc acatgatcaa gttcagagga
2881 cacttcctca tcgagggtga tctcaaccct gataactctg atgtggataa gttgttcatc
2941 cagctcgtgc agacctacaa ccagcttttc gaagagaacc ctatcaacgc ttcaggtgtg
3001 gatgctaagg ctatcctctc tgctaggctc tctaagtcaa gaaggcttga gaacctcatt
3061 gctcagctcc ctggtgagaa gaagaacgga cttttcggaa acttgatcgc tctctctctc
3121 ggactcaccc ctaacttcaa gtctaacttc gatctcgctg aggatgcaaa gctccagctc
3181 tcaaaggata cctacgatga tgatctcgat aacctcctcg ctcagatcgg agatcagtac
3241 gctgatttgt tcctcgctgc taagaacctc tctgatgcta tcctcctcag tgatatcctc
3301 agagtgaaca ccgagatcac caaggctcca ctctcagctt ctatgatcaa gagatacgat
3361 gagcaccacc aggatctcac acttctcaag gctcttgtta gacagcagct cccagagaag
3421 tacaaagaga ttttcttcga tcagtctaag aacggatacg ctggttacat cgatggtggt
3481 gcatctcaag aagagttcta caagttcatc aagcctatcc tcgagaagat ggatggaacc
3541 gaggaactcc tcgtgaagct caatagagag gatcttctca gaaagcagag gaccttcgat
3601 aacggatcta tccctcatca gatccacctc ggagagttgc acgctatcct tagaaggcaa
3661 gaggatttct acccattcct caaggataac agggaaaaga ttgagaagat tctcaccttc
3721 agaatccctt actacgtggg acctctcgct agaggaaact caagattcgc ttggatgacc
3781 agaaagtctg aggaaaccat caccccttgg aacttcgaag aggtggtgga taagggtgct
3841 agtgctcagt ctttcatcga gaggatgacc aacttcgata agaaccttcc aaacgagaag
3901 gtgctcccta agcactcttt gctctacgag tacttcaccg tgtacaacga gttgaccaag
3961 gttaagtacg tgaccgaggg aatgaggaag cctgcttttt tgtcaggtga gcaaaagaag
4021 gctatcgttg atctcttgtt caagaccaac agaaaggtga ccgtgaagca gctcaaagag
4081 gattacttca agaaaatcga gtgcttcgat tcagttgaga tttctggtgt tgaggatagg
4141 ttcaacgcat ctctcggaac ctaccacgat ctcctcaaga tcattaagga taaggatttc
4201 ttggataacg aggaaaacga ggatatcttg gaggatatcg ttcttaccct caccctcttt
4261 gaagatagag agatgattga agaaaggctc aagacctacg ctcatctctt cgatgataag
4321 gtgatgaagc agttgaagag aagaagatac actggttggg gaaggctctc aagaaagctc
4381 attaacggaa tcagggataa gcagtctgga aagacaatcc ttgatttcct caagtctgat
4441 ggattcgcta acagaaactt catgcagctc atccacgatg attctctcac ctttaaagag
4501 gatatccaga aggctcaggt ttcaggacag ggtgatagtc tccatgagca tatcgctaac
4561 ctcgctggat ctcctgcaat caagaaggga atcctccaga ctgtgaaggt tgtggatgag
4621 ttggtgaagg tgatgggaag gcataagcct gagaacatcg tgatcgaaat ggctagagag
4681 aaccagacca ctcagaaggg acagaagaac tctagggaaa ggatgaagag gatcgaggaa
4741 ggtatcaaag agcttggatc tcagatcctc aaagagcacc ctgttgagaa cactcagctc
4801 cagaatgaga agctctacct ctactacctc cagaacggaa gggatatgta tgtggatcaa
4861 gagttggata tcaacaggct ctctgattac gatgttgatc atatcgtgcc acagtcattc
4921 ttgaaggatg attctatcga taacaaggtg ctcaccaggt ctgataagaa caggggtaag
4981 agtgataacg tgccaagtga agaggttgtg aagaaaatga agaactattg gaggcagctc
5041 ctcaacgcta agctcatcac tcagagaaag ttcgataact tgactaaggc tgagagggga
5101 ggactctctg aattggataa ggcaggattc atcaagaggc agcttgtgga aaccaggcag
5161 atcactaagc acgttgcaca gatcctcgat tctaggatga acaccaagta cgatgagaac
5221 gataagttga tcagggaagt gaaggttatc accctcaagt caaagctcgt gtctgatttc
5281 agaaaggatt tccaattcta caaggtgagg gaaatcaaca actaccacca cgctcacgat
5341 gcttacctta acgctgttgt tggaaccgct ctcatcaaga agtatcctaa gctcgagtca
5401 gagttcgtgt acggtgatta caaggtgtac gatgtgagga agatgatcgc taagtctgag
5461 caagagatcg gaaaggctac cgctaagtat ttcttctact ctaacatcat gaatttcttc
5521 aagaccgaga ttaccctcgc taacggtgag atcagaaaga ggccactcat cgagacaaac
5581 ggtgaaacag gtgagatcgt gtgggataag ggaagggatt tcgctaccgt tagaaaggtg
5641 ctctctatgc cacaggtgaa catcgttaag aaaaccgagg tgcagaccgg tggattctct
5701 aaagagtcta tcctccctaa gaggaactct gataagctca ttgctaggaa gaaggattgg
5761 gaccctaaga aatacggtgg tttcgattct cctaccgtgg cttactctgt tctcgttgtg
5821 gctaaggttg agaagggaaa gagtaagaag ctcaagtctg ttaaggaact tctcggaatc
5881 actatcatgg aaaggtcatc tttcgagaag aacccaatcg atttcctcga ggctaaggga
5941 tacaaagagg ttaagaagga tctcatcatc aagctcccaa agtactcact cttcgaactc
6001 gagaacggta gaaagaggat gctcgcttct gctggtgagc ttcaaaaggg aaacgagctt
6061 gctctcccat ctaagtacgt taactttctt tacctcgctt ctcactacga gaagttgaag
6121 ggatctccag aagataacga gcagaagcaa cttttcgttg agcagcacaa gcactacttg
6181 gatgagatca tcgagcagat ctctgagttc tctaaaaggg tgatcctcgc tgatgcaaac
6241 ctcgataagg tgttgtctgc ttacaacaag cacagagata agcctatcag ggaacaggca
6301 gagaacatca tccatctctt cacccttacc aacctcggtg ctcctgctgc tttcaagtac
6361 ttcgatacaa ccatcgatag gaagagatac acctctacca aagaagtgct cgatgctacc
6421 ctcatccatc agtctatcac tggactctac gagactagga tcgatctctc acagctcggt
6481 ggtgattcaa gggctgatcc taagaagaag aggaaggttt gactcgagat atgaagatga
6541 agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc ttaagtttgt gtttttttct
6601 tggcttgttg tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt aagatcacat
6661 tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg atctcttctg
6721 cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta aagataagga
6781 gctccggtga cggacggcgc gctggcagac atactgtccc acaaatgaag atggaatctg
6841 taaaagaaaa cgcgtgaaat aatgcgtctg acaaaggtta ggtcggctgc ctttaatcaa
6901 taccaaagtg gtccctacca cgatggaaaa actgtgcagt cggtttggct ttttctgacg
6961 aacaaataag attcgtggcc gacaggtggg ggtccaccat gtgaaggcat cttcagactc
7021 caataatgga gcaatgacgt aagggcttac gaaataagta agggtagttt gggaaatgtc
7081 cactcacccg tcagtctata aatacttagc ccctccctca ttgttaaggg agcaaaatct
7141 cagagagata gtcctagaga gagaaagaga gcaagtagcc tagaagtagt caaggcggcg
7201 aagtattcag gcacgtggcc aggaagaaga aaagccaaga cgacgaaaac aggtaagagc
7261 taagcttcct gcaggttcac tgccgtatag gcagatgata gatccagatg ttccgtttta
7321 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
7381 gagtcggtgc gttcactgcc gtataggcag aggattgtca cagacattcc gttttagagc
7441 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
7501 cggtgcgttc actgccgtat aggcagttct tcttcttcag acacgagttt tagagctaga
7561 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
7621 gcgttcactg ccgtataggc aggatctctt gaaatcttcg tagttttaga gctagaaata
7681 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgcgt
7741 tcactgccgt ataggcaggg ggcagataca tagtgagggt tttagagcta gaaatagcaa
7801 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgcgttcac
7861 tgccgtatag gcagggtgca ttatattgtt tgctgtttta gagctagaaa tagcaagtta
7921 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc gttcactgcc
7981 gtataggcag ttatcggagc tggccctgct gttttagagc tagaaatagc aagttaaaat
8041 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgcgttc actgccgtat
8101 aggcagggat agttcatcca tcaacagttt tagagctaga aatagcaagt taaaataagg
8161 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcgttcactg ccgtataggc
8221 agcatattct tgaagtttag ctgttttaga gctagaaata gcaagttaaa ataaggctag
8281 tccgttatca acttgaaaaa gtggcaccga gtcggtgcgt tcactgccgt ataggcagag
8341 tccagaagaa gaagaatggt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
8401 ttatcaactt gaaaaagtgg caccgagtcg gtgcgttcac tgccgtatag gcaggagtac
8461 ttacgacgaa tcgagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
8521 caacttgaaa aagtggcacc gagtcggtgc gttcactgcc gtataggcag atgaatcggt
8581 tgtacgcaga gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
8641 ttgaaaaagt ggcaccgagt cggtgcgttc actgccgtat aggcaggtcg atcgacaagc
8701 tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata
8761 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
8821 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc
8881 ccccgaatta agtgccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc
8941 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
9001 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
9061 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
9121 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
9181 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
9241 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
9301 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
9361 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
9421 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
9481 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
9541 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
9601 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
9661 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
9721 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
9781 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
9841 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
9901 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
9961 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
10021 caaggtagtc ggcaaataac cctcgagc
//
