| 质粒名称: | pSt-KO |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 44566 |
| 存储实验室: | Maik Hintze |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1(+) |
| 载体出品公司: | Invitrogen |
| 空载体大小: | 5322bp |
| 完整质粒大小: | 4225bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Lrrk2 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 7700bp |
| 基因突变: | 无 |
| 启动子: | CMV |
| 标签/融合蛋白: |
StrepII (N terminal on insert)
Myc tag (C terminal on insert)
|
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | NheI (unknown if destroyed) |
| 3’克隆位点: | ApaI (unknown if destroyed) |
| 5’测序引物 | GGC TAA CTA GAG AAC CCA CTG |
| 3’测序引物 |
GGC AAC TAG AAG GCA CAG TC
(Common Sequencing Primers)
|
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3.1(+)_Strep-Lrrk2-Myc was a gift from Maik Hintze (Addgene plasmid # 161582 ; http://n2t.net/addgene:161582 ; RRID:Addgene_161582) |
买家导航
pSt-KO载体质粒基本信息
pSt-KO质粒图谱载体图谱和pSt-KO载体序列质粒序列多克隆位点信息
pSt-KO质粒载体简介
pSt-KO质粒序列载体序列
LOCUS pSt-KO 4225 bp DNA circular SYN 14-APR-2025
DEFINITION for generation of knockout of mycobacterial genes.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4225)
AUTHORS Parikh A, Kumar D, Chawla Y, Kurthkoti K, Khan S, Varshney U,
Nandicoori VK
TITLE Development of a new generation of vectors for gene expression, gene
replacement, and protein-protein interaction studies in
mycobacteria.
JOURNAL Appl Environ Microbiol. 2013 Mar;79(5):1718-29. doi:
10.1128/AEM.03695-12. Epub 2013 Jan 11.
PUBMED 23315736
REFERENCE 2 (bases 1 to 4225)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1128/AEM.03695-12"; journalName: "Appl Environ Microbiol"; date:
"2013-03"; volume: "79"; issue: "5"; pages: "1718-29"
COMMENT Sequence Label: pSt-KO
FEATURES Location/Qualifiers
source 1..4225
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 112..700
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 601..620
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cccttcaccg ttaacactag ttaattaatc tagagcggcc gctgcagggc ccgggatcca
61 tggctagttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
121 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
181 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
241 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
301 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
361 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
421 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
481 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
541 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
601 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
661 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
721 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
781 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
841 aacgaccgag cgcaacgcgt tacactgcat gcggttagga atacggttag ccatttgcct
901 gcttttatat agttatatgg gattcacctt tatgttgata agaaataaaa gaaaatgcca
961 ataggatatc ggcattttct tttgcgtttt tatttgttaa ctgttaattg tccttgttca
1021 aggatgctgt ctttgacaac agatgttttc ttgcctttga tgttcagcag gaagcttggc
1081 gcaaacgttg attgtttgtc tgcgtagaat cctctgtttg tcatatagct tgtaatcacg
1141 acattgtttc ctttcgcttg aggtacagcg aagtgtgagt aagtaaaggt tacatcgtta
1201 ggatcaagat ccatttttaa cacaaggcca gttttgttca gcggcttgta tgggccagtt
1261 aaagaattag aaacataacc aagcatgtaa atatcgttag acgtaatgcc gtcaatcgtc
1321 atttttgatc cgcgggagtc agtgaacagg taccatttgc cgttcatttt aaagacgttc
1381 gcgcgttcaa tttcatctgt tactgtgtta gatgcaatca gcggtttcat cacttttttc
1441 agtgtgtaat catcgtttag ctcaatcata ccgagagcgc cgtttgctaa ctcagccgtg
1501 cgttttttat cgctttgcag aagtttttga ctttcttgac ggaagaatga tgtgcttttg
1561 ccatagtatg ctttgttaaa taaagattct tcgccttggt agccatcttc agttccagtg
1621 tttgcttcaa atactaagta tttgtggcct ttatcttcta cgtagtgagg atctctcagc
1681 gtatggttgt cgcctgagct gtagttgcct tcatcgatga actgctgtac attttgatac
1741 gtttttccgt caccgtcaaa gattgattta taatcctcta caccgttgat gttcaaagag
1801 ctgtctgatg ctgatacgtt aacttgtgca gttgtcagtg tttgtttgcc gtaatgttta
1861 ccggagaaat cagtgtagaa taaacggatt tttccgtcag atgtaaatgt ggctgaacct
1921 gaccattctt gtgtttggtc ttttaggata gaatcatttg catcgaattt gtcgctgtct
1981 ttaaagacgc ggccagcgtt tttccagctg tcaatagaag tttcgccgac tttttgatag
2041 aacatgtaaa tcgatgtgtc atccgcattt ttaggatctc cggctaatgc aaagacgatg
2101 tggtagccgt gatagtttgc gacagtgccg tcagcgtttt gtaatggcca gctgtcccaa
2161 acgtccaggc cttttgcaga agagatattt ttaattgtgg acgaatcgaa ttcaggaact
2221 tgatattttt catttttttg ctgttcaggg atttgcagca tatcatggcg tgtaatatgg
2281 gaaatgccgt atgtttcctt atatggcttt tggttcgttt ctttcatatg cgcaaacgct
2341 tgagttgcgc ctcctgccag cagtgcggta gtaaaggtta atactgttgc ttgttttgca
2401 aactttttga tgttcatcgt tcatgtctcc ttttttatgt actgtgttag cggtctgctt
2461 cttccagccc tcctgtttga agatggcaag ttagttacgc acaataaaaa aagacctaaa
2521 atatgtaagg ggtgacgcca aagtatcgac ctcgagtcac cgggtgacgg caaccccgct
2581 acttacgctg gccgggcgca gtgcccgcga cggacggctc aagttgtcct cgctgccact
2641 cgctgcgacg acgggcctgg cctcaccgtc ccgacctacg actcaccggt cgcgagtgcc
2701 aacgttattc ttagcactcg cctatgccga gtgcaagaag ccccgcaccg ggtcatgccc
2761 ctcgttcgac cttgtcctcg gccctccgat ccgggtgagt atgttcggcc catgaccgcc
2821 aacgacaaca agacccgtaa atggtcggcc gcagacgtcc ccgatcaaag cgggcgcgtc
2881 gttgtggtca cgcatgcggt ggtaccttga tccggcatga gattatcaaa aaggatcttc
2941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
3001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
3061 tttcgttcat ccatagttgc ctgactcccc gacgtggccg accagcccgt catcgtcaac
3121 gcctgaccgc ggtgcggaca ggccgtgtcg cgaccggccg tgcggaatta agccggcccg
3181 taccctgtga atagaggtcc gctgtgacac aagaatccct gttacttctc gaccgtattg
3241 attcggatga ttcctacgcg agcctgcgga acgaccagga gttctgggag ccgctggccc
3301 gccgagccct ggaggagctc gggctgccgg tgccgccggt gctgcgggtg cccggcgaga
3361 gcaccaaccc cgtactggtc ggcgagcccg acccggtgat caagctgttc ggcgagcact
3421 ggtgcggtcc ggagagcctc gcgtcggagt cggaggcgta cgcggtcctg gcggacgccc
3481 cggtgccggt gccccgcctc ctcggccgcg gcgagctgcg gcccggcacc ggagcctggc
3541 cgtggcccta cctggtgatg agccggatga ccggcaccac ctggcggtcc gcgatggacg
3601 gcacgaccga ccggaacgcg ctgctcgccc tggcccgcga actcggccgg gtgctcggcc
3661 ggctgcacag ggtgccgctg accgggaaca ccgtgctcac cccccattcc gaggtcttcc
3721 cggaactgct gcgggaacgc cgcgcggcga ccgtcgagga ccaccgcggg tggggctacc
3781 tctcgccccg gctgctggac cgcctggagg actggctgcc ggacgtggac acgctgctgg
3841 ccggccgcga accccggttc gtccacggcg acctgcacgg gaccaacatc ttcgtggacc
3901 tggccgcgac cgaggtcacc gggatcgtcg acttcaccga cgtctatgcg ggagactccc
3961 gctacagcct ggtgcaactg catctcaacg ccttccgggg cgaccgcgag atcctggccg
4021 cgctgctcga cggggcgcag tggaagcgga ccgaggactt cgcccgcgaa ctgctcgcct
4081 tcaccttcct gcacgacttc gaggtgttcg aggagacccc gctggatctc tccggcttca
4141 ccgatccgga ggaactggcg cagttcctct gggggccgcc ggacaccgcc cccggcgcct
4201 gacgcccccc ggatcagtgg ccgcc
//
