pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)

LOCUS      pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)       10266 bp    DNA     circular SYN 14-APR-2025
DEFINITION  For the Supernova, a series of vector systems for single-cell 
            labeling and gene function sudies in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Mizuno H, Luo W, Tarusawa E, Saito YM, Sato T, Yoshimura Y, Itohara 
            S, Iwasato T
  TITLE     NMDAR-regulated dynamics of layer 4 neuronal dendrites during 
            thalamocortical reorganization in neonates.
  JOURNAL   Neuron. 2014 Apr 16;82(2):365-79. doi: 10.1016/j.neuron.2014.02.026.
            Epub 2014 Mar 27.
  PUBMED    24685175
REFERENCE   2  (bases 1 to 10266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.02.026"; journalName: "Neuron"; date: 
            "2014-04-16- 16"; volume: "82"; issue: "2"; pages: "365-79"
COMMENT     Sequence Label: pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
            (Su...
FEATURES             Location/Qualifiers
     source          1..10266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        392..667
                     /label=chicken beta-actin promoter
     intron          668..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     protein_bind    complement(1733..1766)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          2300..2365
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2495..2515
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2940..3074
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2977..2996)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3031..3050
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3116..3149)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3192..3887
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     primer_bind     complement(4085..4102)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4312..4331
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4484..5101,5102..5107,5108..5143,5144..5146,5147..5182,
                     5183..5185,5186..5221,5222..5230)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5317..5337)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5735..5746)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5875..5894)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5940..5995
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5994..6013)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(6378..6966)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6458..6477)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7295..7342
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7367..7386
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7574..8362)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7685..7704)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8289..8308
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(8397..8757)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8411..8549
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        8566..8757
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8714..8734
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(8817..9608,9609..9677))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9440..9459
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9678..9782)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9809..10264
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9896..9915)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10106..10127
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg gtcgaggtga gccccacgtt ctgcttcact
      421 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
      481 tgtgcagcga tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga
      541 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
      601 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
      661 gcgggcggga gtcgctgcgt tgccttcgcc ccgtgccccg ctccgcgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctc gtttcttttc tgtggctgcg
      841 tgaaagcctt aaagggctcc gggagggccc tttgtgcggg ggggagcggc tcggggggtg
      901 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggccc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcgtg tgcgcgaggg gagcgcggcc
     1021 gggggcggtg ccccgcggtg cgggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcggcggt cgggctgtaa cccccccctg
     1141 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtgcgggg
     1201 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     1261 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctggc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccatc tccagcctcg gggctgccgc
     1561 agggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttgtt gtgctgtctc atcattttgg caaagaattc taataacttc
     1741 gtatagcata cattatacga agttatatta agggttccgg atcctcgggg acaccaaata
     1801 tggcgatctc ggccttttcg tttcttggag ctgggacatg tttgccatcg atccatctac
     1861 caccagaacg gccgttagat ctgctgccac cgttgtttcc accgaagaaa ccaccgttgc
     1921 cgtaaccacc acgacggttg ttgctaaaga agctgccacc gccacggcca ccgttgtagc
     1981 cgccgttgtt gttattgtag ttgctactgt tatttctggc acttcttggt tttcctctta
     2041 agtgaggagg aacataacca ttctcgttgt tgtcgttgat gcttaaattt tgcacttgtt
     2101 cgctcagttc agccataata tgaaatgctt ttcttgttgt tcttacggaa taccacttgc
     2161 cacctatcac cacaactaac tttttcccgt tcctccatct cttttatatt ttttttctcg
     2221 agggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
     2281 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
     2341 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
     2401 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
     2461 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
     2521 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
     2581 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
     2641 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
     2701 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
     2761 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
     2821 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     2881 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     2941 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3001 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3061 atcatgtctg gatctgacat ggtaagtaag cttgggctgc aggtcgaggg acctaataac
     3121 ttcgtatagc atacattata cgaagttata tgtcgacggt accgcgggcc cgggatccac
     3181 cggtcgccac catgagcgag ctgatcaagg agaacatgca catgaagctg tacatggagg
     3241 gcaccgtgaa caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg
     3301 gcacccagac catgaagatc aaggtggtcg agggcggccc tctccccttc gccttcgaca
     3361 tcctggctac cagcttcatg tacggcagca aagccttcat caaccacacc cagggcatcc
     3421 ccgacttctt taagcagtcc ttccctgagg gcttcacatg ggagagaatc accacatacg
     3481 aagacggggg cgtgctgacc gctacccagg acaccagctt ccagaacggc tgcatcatct
     3541 acaacgtcaa gatcaacggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa
     3601 cacgcggctg ggaggccaac accgagatgc tgtaccccgc tgacggcggc ctgagaggcc
     3661 acagccagat ggccctgaag ctcgtgggcg ggggctacct gcactgctcc ttcaagacca
     3721 catacagatc caagaaaccc gctaagaacc tcaagatgcc cggcttccac ttcgtggacc
     3781 acagactgga aagaatcaag gaggccgaca aagagaccta cgtcgagcag cacgagatgg
     3841 ctgtggccaa gtactgcgac ctccctagca aactggggca cagatgatgc tctagatcga
     3901 gttaattaag gattccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc
     3961 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     4021 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     4081 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     4141 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     4201 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     4261 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     4321 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     4381 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     4441 ggccccccga accacgggga cgtggtttcc tttgaaaaac acgatgtcta gactggacaa
     4501 gagcaaagtc ataaactctg ctctggaatt actcaatgaa gtcggtatcg aaggcctgac
     4561 gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa
     4621 gaacaagcgg gccctgctcg atgccctggc aatcgagatg ctggacaggc atcataccca
     4681 cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcatt
     4741 ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac
     4801 agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc
     4861 cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt
     4921 ggaggatcag gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat
     4981 gcccccactt ctgagacaag caattgagct gttcgaccat cagggagccg aacctgcctt
     5041 ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg
     5101 cgggccggcc gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga
     5161 cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct
     5221 ccccgggtaa ctaagtaagc ggccgcgaat tcgcccttcg gccgctcaac ctctggatta
     5281 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     5341 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     5401 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     5461 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     5521 cacctgtcag ctcctttccg gaactttcgc tttccccctc cctattgcca cggcggaact
     5581 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     5641 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg
     5701 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     5761 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     5821 gagtcggatc tccctttggg ccgcctcccc gcagcggccg cggcgactcg aggatctttt
     5881 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta
     5941 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     6001 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt
     6061 ggcaacatat gccatatgct ggctgccatg aacaaaggtg gctataaaga ggtcatcagt
     6121 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
     6181 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
     6241 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
     6301 ctcttctctt atgaagccat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6361 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6421 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6481 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6541 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6601 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6661 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6721 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6781 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     6841 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6901 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6961 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7021 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7081 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aacctgaggc tatggcaggg
     7141 cctgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg ggggtggggt
     7201 ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca
     7261 gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt
     7321 ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt ctccttccgt
     7381 gtttcagtta gcctccccct agggtgggcg aagaactcca gcatgagatc cccgcgctgg
     7441 aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg
     7501 gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaac
     7561 cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat
     7621 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt
     7681 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc
     7741 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat
     7801 cgccatgggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca
     7861 gttcggctgg cgcgagcccc tgatgctctt gatcatcctg atcgacaaga ccggcttcca
     7921 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
     7981 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
     8041 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc
     8101 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg
     8161 atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa
     8221 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg
     8281 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt
     8341 gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcgatctttg
     8401 caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag
     8461 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg
     8521 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct
     8581 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
     8641 cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
     8701 ggggactttc cacaccctaa ctgacacaca ttccacagct ggttctttcc gcctcaggac
     8761 tcttcctttt tcaataaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8821 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8881 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8941 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9001 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9061 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9121 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9181 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9241 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9301 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9361 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9421 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9481 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9541 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9601 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9661 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9721 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9781 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
     9841 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     9901 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     9961 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    10021 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    10081 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    10141 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    10201 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
    10261 atttac
//