| 质粒名称: | pMSCV-FlagMLL-pl-ENL(5613) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 20873 |
| 存储实验室: | Robert Slany |
|
载体骨架基本信息 |
|
| 载体名称: | pMSCV-neo |
| 载体出品公司: | Clontech |
| 空载体大小: | 6500 bp |
| 完整质粒大小: | 11106 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 新霉素（G418筛选） |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | MLL |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 4580 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | Flag（插入时的N端子） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRI (未破坏) |
| 3’克隆位点: | EcoRI(未破坏) |
| 5’测序引物 | MSCV |
| 3’测序引物 | MSCV-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pMSCV-FlagMLL-pl-ENL(5613) was a gift from Robert Slany (Addgene plasmid # 20873 ; http://n2t.net/addgene:20873 ; RRID:Addgene_20873) |
| 参考文献部分: | Alterations of the CxxC domain preclude oncogenic activation of mixed-lineage leukemia 2. Bach C, Mueller D, Buhl S, Garcia-Cuellar MP, Slany RK. Oncogene. 2009 Feb 12. 28(6):815-23. 10.1038/onc.2008.443 PubMed 19060922 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pMSCV-FlagMLL-pl-ENL(5613) 11106 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pMSCV-FlagMLL-pl-ENL(5613)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11106)
AUTHORS Bach C, Mueller D, Buhl S, Garcia-Cuellar MP, Slany RK
TITLE Alterations of the CxxC domain preclude oncogenic activation of
mixed-lineage leukemia 2.
JOURNAL Oncogene. 2009 Feb 12. 28(6):815-23.
PUBMED 19060922
REFERENCE 2 (bases 1 to 11106)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..11106
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 579..920
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 987..1403
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1333..1355
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1371..1387
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS 1415..1438
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
primer_bind complement(6074..6090)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6075..6091)
/label=pBluescriptKS
/note="For pBluescript vector"
promoter 6108..6607
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(6143..6166)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 6540..6559
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 6621..7424
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6684..6703)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7294..7313
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
LTR 7499..8013
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind 8175..8191
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8199..8229)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8244..8265
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8382..8399)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8553..9141)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8633..8652)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9312..10172)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9935..9954
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10173..10277)
/gene="bla"
/label=AmpR promoter
primer_bind 10345..10363
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(10401..10423)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 10523..10542
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 10736..10758
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 10886..10905
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
LTR 11105..515
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
ORIGIN
1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
1381 ccctcactcc ttctctaggc gccggaattc catggactac aaggacgacg atgacaaggg
1441 caattccgcg aacatggcgc acagctgtcg gtggcgcttc cccgcccgac ccgggaccac
1501 cgggggcggc ggcggcgggg ggcgccgggg cctagggggc gccccgcggc aacgcgtccc
1561 ggccctgctg cttccccccg ggcccccggt cggcggtggc ggccccgggg cgcccccctc
1621 ccccccggct gtggcggccg cggcggcggc ggcgggaagc agcggggctg gggttccagg
1681 gggagcggcc gccgcctcag cagcctcctc gtcgtccgcc tcgtcttcgt cttcgtcatc
1741 gtcctcagcc tcttcagggc cggccctgct ccgggtgggc ccgggcttcg acgcggcgct
1801 gcaggtctcg gccgccatcg gcaccaacct gcgccggttc cgggccgtgt ttggggagag
1861 cggcggggga ggcggcagcg gagaggatga gcaattctta ggttttggct cagatgaaga
1921 agtcagagtg cgaagtccca caaggtctcc ttcagttaaa actagtcctc gaaaacctcg
1981 tgggagacct agaagtggct ctgaccgaaa ttcagctatc ctctcagatc catctgtgtt
2041 ttcccctcta aataaatcag agaccaaatc tggagataag atcaagaaga aagattctaa
2101 aagtatagaa aagaagagag gaagacctcc caccttccct ggagtaaaaa tcaaaataac
2161 acatggaaag gacatttcag agttaccaaa gggaaacaaa gaagatagcc tgaaaaaaat
2221 taaaaggaca ccttctgcta cgtttcagca agccacaaag attaaaaaat taagagcagg
2281 taaactctct cctctcaagt ctaagtttaa gacagggaag cttcaaatag gaaggaaggg
2341 ggtacaaatt gtacgacgga gaggaaggcc tccatcaaca gaaaggataa agaccccttc
2401 gggtctcctc attaattctg aactggaaaa gccccagaaa gtccggaaag acaaggaagg
2461 aacacctcca cttacaaaag aagataagac agttgtcaga caaagccctc gaaggattaa
2521 gccagttagg attattcctt cttcaaaaag gacagatgca accattgcta agcaactctt
2581 acagagggca aaaaaggggg ctcaaaagaa aattgaaaaa gaagcagctc agctgcaggg
2641 aagaaaggtg aagacacagg tcaaaaatat tcgacagttc atcatgcctg ttgtcagtgc
2701 tatctcctcg cggatcatta agacccctcg gcggtttata gaggatgagg attatgaccc
2761 tccaattaaa attgcccgat tagagtctac accgaatagt agattcagtg ccccgtcctg
2821 tggatcttct gaaaaatcaa gtgcagcttc tcagcactcc tctcaaatgt cttcagactc
2881 ctctcgatct agtagcccca gtgttgatac ctccacagac tctcaggctt ctgaggagat
2941 tcaggtactt cctgaggagc ggagcgatac ccctgaagtt catcctccac tgcccatttc
3001 ccagtcccca gaaaatgaga gtaatgatag gagaagcaga aggtattcag tgtcggagag
3061 aagttttgga tctagaacga cgaaaaaatt atcaactcta caaagtgccc cccagcagca
3121 gacctcctcg tctccacctc cacctctgct gactccaccg ccaccactgc agccagcctc
3181 cagtatctct gaccacacac cttggcttat gcctccaaca atccccttag catcaccatt
3241 tttgcctgct tccactgctc ctatgcaagg gaagcgaaaa tctattttgc gagaaccgac
3301 atttaggtgg acttctttaa agcattctag gtcagagcca caatactttt cctcagcaaa
3361 gtatgccaaa gaaggtctta ttcgcaaacc aatatttgat aatttccgac cccctccact
3421 aactcccgag gacgttggct ttgcatctgg tttttctgca tctggtaccg ctgcttcagc
3481 ccgattgttt tcgccactcc attctggaac aaggtttgat atgcacaaaa ggagccctct
3541 tctgagagct ccaagattta ctccaagtga ggctcactct agaatatttg agtctgtaac
3601 cttgcctagt aatcgaactt ctgctggaac atcttcttca ggagtatcca atagaaaaag
3661 gaaaagaaaa gtgtttagtc ctattcgatc tgaaccaaga tctccttctc actccatgag
3721 gacaagaagt ggaaggctta gtagttctga gctctcacct ctcacccccc cgtcttctgt
3781 ctcttcctcg ttaagcattt ctgttagtcc tcttgccact agtgccttaa acccaacttt
3841 tacttttcct tctcattccc tgactcagtc tggggaatct gcagagaaaa atcagagacc
3901 aaggaagcag actagtgctc cggcagagcc attttcatca agtagtccta ctcctctctt
3961 cccttggttt accccaggct ctcagactga aagagggaga aataaagaca aggcccccga
4021 ggagctgtcc aaagatcgag atgctgacaa gagcgtggag aaggacaaga gtagagagag
4081 agaccgggag agagaaaagg agaataagcg ggagtcaagg aaagagaaaa ggaaaaaggg
4141 atcagaaatt cagagtagtt ctgctttgta tcctgtgggt agggtttcca aagagaaggt
4201 tgttggtgaa gatgttgcca cttcatcttc tgccaaaaaa gcaacagggc ggaagaagtc
4261 ttcatcacat gattctggga ctgatattac ttctgtgact cttggggata caacagctgt
4321 caaaaccaaa atacttataa agaaagggag aggaaatctg gaaaaaacca acttggacct
4381 cggcccaact gccccatccc tggagaagga gaaaaccctc tgcctttcca ctccttcatc
4441 tagcactgtt aaacattcca cttcctccat aggctccatg ttggctcagg cagacaagct
4501 tccaatgact gacaagaggg ttgccagcct cctaaaaaag gccaaagctc agctctgcaa
4561 gattgagaag agtaagagtc ttaaacaaac cgaccagccc aaagcacagg gtcaagaaag
4621 tgactcatca gagacctctg tgcgaggacc ccggattaaa catgtctgca gaagagcagc
4681 tgttgccctt ggccgaaaac gagctgtgtt tcctgatgac atgcccaccc tgagtgcctt
4741 accatgggaa gaacgagaaa agattttgtc ttccatgggg aatgatgaca agtcatcaat
4801 tgctggctca gaagatgctg aacctcttgc tccacccatc aaaccaatta aacctgtcac
4861 tagaaacaag gcaccccagg aacctccagt aaagaaagga cgtcgatcga ggcggtgtgg
4921 gcagtgtccc ggctgccagg tgcctgagga ctgtggtgtt tgtactaatt gcttagataa
4981 gcccaagttt ggtggtcgca atataaagaa gcagtgctgc aagatgagaa aatgtcagaa
5041 tctacaatgg atgccttcca aagcctacct gcagaagcaa gctaaagctg tgaaaaagaa
5101 agagaaaaag tctaagacca gtgaaaagaa agacagcaaa gagagcagtg ttgtgaagaa
5161 cgtggtggac tctagtcaga aacctacccc atcagcaaga gaggatcctg ccccaaagaa
5221 aagcagtagt gagcctcctc cacgaaagcc cgtcgaggaa aagagtgaag aagggaatgt
5281 ctcggcccct gggcctgaat ccaaacaggc caccactcca gcttccagga agtcaagcaa
5341 gcaggtctcc cagccagcac tggtcatccc gcctcagcca cctactacag gaccgccaag
5401 aaaagaagtt cccaaaacca ctcctagtga gcccaagaaa aagcagcctc caccaccaga
5461 atcaggtcca gagcagagca aacagaaaaa agtggctccc cgcccaagta tccctgtaaa
5521 acaaaaacca aaagaaaagg aaaaaccacc tccggtcaat aagcaggaga atgcaggcac
5581 tttgaacatc ctcagcactc tctccaatgg caatagttct aagcaaaaaa ttccagcaga
5641 tgggtcgcga agtatacaca cggggaggga ctccaggttg agcttcagcg acagcgagag
5701 tgacaacagc gccgactcct ccctgcccag ccgtgagccc ccaccccccc agaagccacc
5761 cccgcccaac agcaaggtgt caggccggag gagccccgag tcctgcagca agcctgagaa
5821 gatcctcaag aagggcacct acgacaaggc ctacacggat gagctggtgg agctacaccg
5881 gaggctgatg gcgctgcggg agcgcaacgt gctgcagcag attgtgaatc tgatcgagga
5941 gactggccac ttcaatgtca ccaacaccac cttcgacttc gacctcttct ccctggacga
6001 gaccaccgtg cgcaaactgc agagctgcct ggaggccgtg gccacatgac cctgaattcg
6061 atatcaagct tatcgatacc gtcgacctcg agagatctaa ttctaccggg taggggaggc
6121 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
6181 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
6241 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
6301 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
6361 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
6421 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
6481 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
6541 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
6601 cctttcgacc tgcagccaat atgggatcgg ccattgaaca agatggattg cacgcaggtt
6661 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
6721 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
6781 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
6841 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
6901 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
6961 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
7021 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
7081 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
7141 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
7201 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
7261 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
7321 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
7381 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaggggat ccgtcgacct
7441 gcagccaagc ttatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
7501 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
7561 gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga gagacagcag
7621 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
7681 cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat cagatgtttc
7741 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
7801 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
7861 tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg tatccaataa
7921 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
7981 gtgattgact acccgtcagc gggggtcttt catgggtaac agtttcttga agttggagaa
8041 caacattctg agggtaggag tcgaatatta agtaatcctg actcaattag ccactgtttt
8101 gaatccacat actccaatac tcctgaaata gttcattatg gacagcgcag aaagagctgg
8161 ggagaattgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
8221 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
8281 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
8341 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
8401 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
8461 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
8521 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
8581 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
8641 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
8701 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
8761 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
8821 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
8881 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
8941 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
9001 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
9061 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
9121 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
9181 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
9241 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
9301 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
9361 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
9421 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
9481 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
9541 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
9601 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
9661 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
9721 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
9781 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
9841 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
9901 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
9961 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
10021 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
10081 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
10141 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
10201 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
10261 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
10321 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
10381 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
10441 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
10501 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
10561 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
10621 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
10681 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
10741 tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg caaggagatg gcgcccaaca
10801 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
10861 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
10921 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggcgatta gtccaatttg
10981 ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga
11041 agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc cagaaaaagg
11101 ggggaa
//
