| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-PTuner2 |
| 质粒类型: | 慢病毒载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 8538 bp |
| 5' 测序引物及序列: | CMV Forward： CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | DD tag (N-端） |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: |
慢毒载体pLVX-PTuner2载体表达N端DD标签融合蛋白； DD标签是一个大小为12KD的FKBP (L106P)去稳定结构域，带有DD标签的目的蛋白将很快被降解； 向培养基中加入Shield1配体后，情况将发生逆转，细胞中目的蛋白的量则迅速提高。 |
| 产品目录号: | 631096 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
pLVX-PTuner2载体质粒基本信息
pLVX-PTuner2质粒图谱载体图谱和pLVX-PTuner2载体序列质粒序列多克隆位点信息
pLVX-PTuner2质粒载体简介
pLVX-PTuner2质粒序列载体序列
LOCUS pLVX-PTuner2 8538 bp DNA circular 11-APR-2011
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT Cat. No. 631096,631092
FEATURES Location/Qualifiers
LTR 1..635
/label=5'\LTR
misc_feature 685..822
/label=Packaging\signal
misc_feature 2028..2151
/label=cPPT/CTS\(DNA\flap)
promoter 2185..2787
/label=CMV\IE\Promoter
misc_feature 4506..5097
/label=WPRE
rep_origin complement(6406..7079)
/label=pUC\origin
CDS complement(7224..8220)
/label=Amp(R)
misc_binding 1303..1536
/label=RRE
misc_binding 636..653
/label=PBS
LTR 5300..5936
/label=3'\LTR
misc_feature 5336..5735
/label=deletion
misc_feature 3364..3872
/label=PGK\Promoter
misc_feature 3893..4492
/label=Puro(R)
CDS 2802..3278
/label=DD-G
BASE COUNT 2134 a 2158 c 2245 g 2001 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tcgctagcgc tatgatcagt ctgattgcgg
2821 cgttagcggt agatctcgtt atcggcatgg aaaacgccat gccgtggaac ctgcctgccg
2881 atctcgcctg gtttaaacgc aacaccttaa ataaacccgt gattatgggc cgccatacct
2941 gggaatcaat cggtcgtccg ttgccaggac gcaaaaatat tatcctcagc agtcaaccga
3001 gtacggacga tcgcgtaacg tgggtgaagt cggtggatga agccatcgcg gcgtgtggtg
3061 acgtaccaga aatcatggtg attggcggcg gtcgcgttat tgaacagttc ttgccaaaag
3121 cgcaaaaact gtatctgacg catatcgacg cagaagtgga aggcgacact catttcccgg
3181 attacgagcc ggatgactgg gaatcggtat tcagcgaatt ccacgatgct gatgcgcaga
3241 actctcacag ctattgcttt gagattctgg agcggcgaag cgctaccgga ctcagatctc
3301 gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc cgcgactcta
3361 gataattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
3421 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
3481 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
3541 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
3601 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
3661 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
3721 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
3781 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
3841 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga
3901 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
3961 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
4021 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
4081 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
4141 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
4201 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
4261 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
4321 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
4381 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
4441 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct
4501 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
4561 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
4621 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
4681 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
4741 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
4801 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
4861 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
4921 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
4981 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
5041 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
5101 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
5161 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
5221 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
5281 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct
5341 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
5401 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
5461 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
5521 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
5581 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
5641 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
5701 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
5761 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
5821 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
5881 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
5941 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
6001 agaggccttg acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca
6061 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
6121 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
6181 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
6241 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
6301 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
6361 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
6421 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
6481 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
6541 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
6601 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
6661 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
6721 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
6781 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
6841 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
6901 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
6961 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
7021 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
7081 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
7141 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
7201 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
7261 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
7321 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
7381 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
7441 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
7501 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
7561 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
7621 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
7681 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
7741 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
7801 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
7861 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
7921 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
7981 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
8041 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
8101 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
8161 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
8221 gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag
8281 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
8341 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
8401 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
8461 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
8521 tactacaaac ttagtagt
//
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