PL-SIN-Oct4-EGFP

LOCUS       PL-SIN-Oct4-EGFP	8324 bp 	DNA	SYN
DEFINITION  PL-SIN-Oct4-EGFP
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8324
                     /organism="PL-SIN-Oct4-EGFP"
                     /mol_type="other DNA"
     misc_feature    complement(29..51)
                     /label="pGEX_3_primer"
     misc_feature    complement(250..398)
                     /label="lacZ_a"
     misc_feature    364..386
                     /label="M13_pUC_fwd_primer"
     promoter        379..395
                     /label="M13_forward20_primer"
     misc_feature    862..1042
                     /label="HIV-1_5_LTR"
     misc_feature    862..1042
                     /label="truncHIV-1_3_LTR"
     misc_feature    1153..1197
                     /label="HIV-1_psi_pack"
     CDS             1198..1659
                     /label="ORF frame 1"
     misc_feature    1697..1712
                     /label="cPPT"
     CDS             2078..2767
                     /label="ORF frame 2"
     misc_feature    2200..2433
                     /label="RRE"
     CDS             complement(4233..4808)
                     /label="ORF frame 1"
     CDS             4708..5484
                     /label="ORF frame 1"
     gene            4768..5481
                     /label="EGFP"
                     /gene="EGFP"
     misc_feature    complement(4810..4831)
                     /label="EGFP_N_primer"
     misc_feature    4948..4977
                     /label="Y66 (EGFP)"
     misc_feature    5418..5439
                     /label="EGFP_C_primer"
     misc_feature    5491..5507
                     /label="pBluescriptSK_primer"
     misc_feature    5594..5609
                     /label="cPPT"
     misc_feature    5594..5615
                     /label="U3PPT"
     misc_feature    5664..5844
                     /label="truncHIV-1_3_LTR"
     misc_feature    5664..5844
                     /label="HIV-1_5_LTR"
     misc_feature    complement(5881..5903)
                     /label="M13_pUC_rev_primer"
     promoter        complement(5917..5946)
                     /label="lac_promoter"
     rep_origin      complement(6255..6874)
                     /label="pBR322_origin"
     gene            complement(7029..7888)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(7313..7888)
                     /label="ORF frame 2"
     promoter        complement(7930..7958)
                     /label="AmpR_promoter"
     misc_feature    complement(8098..8117)
                     /label="SV40pro_F_primer"
     rep_origin      complement(8102..8179)
                     /label="SV40_origin"
ORIGIN
    1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
   61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
  121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
  181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
  241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
  301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
  361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT GCGGCCGCTG GAAGGGCTAA
  421 TTTGGTCCCA AAAAAGACAA GAGATCCTTG ATCTGTGGAT CTACCACACA CAAGGCTACT
  481 TCCCTGATTG GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG ACCTTTGGAT
  541 GGTGCTTCAA GTTAGTACCA GTTGAACCAG AGCAAGTAGA AGAGGCCAAA TAAGGAGAGA
  601 AGAACAGCTT GTTACACCCT ATGAGCCAGC ATGGGATGGA GGACCCGGAG GGAGAAGTAT
  661 TAGTGTGGAA GTTTGACAGC CTCCTAGCAT TTCGTCACAT GGCCCGAGAG CTGCATCCGG
  721 AGTACTACAA AGACTGCTGA CATCGAGCTT TCTACAAGGG ACTTTCCGCT GGGGACTTTC
  781 CAGGGAGGTG TGGCCTGGGC GGGACTGGGG AGTGGCGAGC CCTCAGATGC TACATATAAG
  841 CAGCTGCTTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
  901 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTCAAA
  961 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
 1021 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGTAAAGC
 1081 CAGAGGAGAT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG
 1141 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG
 1201 GGTGCGAGAG CGTCGGTATT AAGCGGGGGA GAATTAGATA AATGGGAAAA AATTCGGTTA
 1261 AGGCCAGGGG GAAAGAAACA ATATAAACTA AAACATATAG TATGGGCAAG CAGGGAGCTA
 1321 GAACGATTCG CAGTTAATCC TGGCCTTTTA GAGACATCAG AAGGCTGTAG ACAAATACTG
 1381 GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT ATATAATACA
 1441 ATAGCAGTCC TCTATTGTGT GCATCAAAGG ATAGATGTAA AAGACACCAA GGAAGCCTTA
 1501 GATAAGATAG AGGAAGAGCA AAACAAAAGT AAGAAAAAGG CACAGCAAGC AGCAGCTGAC
 1561 ACAGGAAACA ACAGCCAGGT CAGCCAAAAT TACCCTATAG TGCAGAACCT CCAGGGGCAA
 1621 ATGGTACATC AGGCCATATC ACCTAGAACT TTAAATTAAG ACAGCAGTAC AAATGGCAGT
 1681 ATTCATCCAC AATTTTAAAA GAAAAGGGGG GATTGGGGGG TACAGTGCAG GGGAAAGAAT
 1741 AGTAGACATA ATAGCAACAG ACATACAAAC TAAAGAATTA CAAAAACAAA TTACAAAAAT
 1801 TCAAAATTTT CGGGTTTATT ACAGGGACAG CAGAGATCCA GTTTGGAAAG GACCAGCAAA
 1861 GCTCCTCTGG AAAGGTGAAG GGGCAGTAGT AATACAAGAT AATAGTGACA TAAAAGTAGT
 1921 GCCAAGAAGA AAAGCAAAGA TCATCAGGGA TTATGGAAAA CAGATGGCAG GTGATGATTG
 1981 TGTGGCAAGT AGACAGGATG AGGATTAACA CATGGAAAAG ATTAGTAAAA CACCATAGCT
 2041 CTAGAGCGAT CCGATCTTCA GACCTGGAGG AGGCGATATG AGGGACAATT GGAGAAGTGA
 2101 ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA GTAGCACCCA CCAAGGCAAA
 2161 GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA GGAGCTTTGT TCCTTGGGTT
 2221 CTTGGGAGCA GCAGGAAGCA CTATGGGCTG CACGTCAATG ACGCTGACGG TACAGGCCAG
 2281 ACAATTATTG TCTGATATAG TGCAGCAGCA GAACAATTTG CTGAGGGCTA TTGAGGCGCA
 2341 ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAACAG CTCCAGGCAA GAATCCTGGC
 2401 TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT CTGGAAAACT
 2461 CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC TGGAACAGAT
 2521 TTGGAATAAC ATGACCTGGA TGGAGTGGGA CAGAGAAATT AACAATTACA CAAGCTTGGT
 2581 AGGTTTAAGA ATAGTTTTTG CTGTACTTTC TATAGTGAAT AGAGTTAGGC AGGGATATTC
 2641 ACCATTATCG TTTCAGACCC ACCTCCCAAT CCCGAGGGGA CCCGACAGGC CCGAAGGAAT
 2701 AGAAGAAGAA GGTGGAGAGA GAGACAGAGA CAGATCCATT CGATTAGTGA ACGGATCAGC
 2761 TTTGTGAACT TGGCGGCTTC CAAGTCGCTG CCTTTATTTA GGTCTTCCAA CTAACCTATG
 2821 GCACTGTTCC ACAATGAATG TATAGAAATT GGGAGGTGAG CATGACAGAG TGGAGGAAAC
 2881 GGAAGATTCA TGGAGAGGGC CAGAGAGATG GCCCCTCAGC CACCCTGGGG GATGACTTGG
 2941 ACCCATGTGG TAGAAGGAGG GGACTTCCAC ACATGTGCTA TGTGTAGCTG TGTGTAGGTA
 3001 CATACACACC CTTAAAATAA AACGCAATTT TTTTTTCAAA GTCTCAGGGT GAATTTGGTG
 3061 AAGTCGATGA AGCTGAGGCA GGAGAATTAT CAGGAGTTCA AGGGCAGCTT GTTTTATAGA
 3121 GAAAGGTTCC ATCTCTACCT GATGAAGACT ACCATCAAGA GACACCCCCG CCCCCCAGGG
 3181 CACCTAGAGC CACTGACCCT AGCCAACAGC TCAGGCGGGC TGGGCCCAGG CTCAGAACTC
 3241 TGTCCTGGCT ATGTACACTG TGGGGTGCTC TGGGCTTTTT GAGGCTGTGT GATTCACCCT
 3301 GGGGCCTTCG TTCAGAGCAT GGTGTAGGAG CAGACAGACA AACACCATCC CTTGCAGACA
 3361 GGCACTCTGA GGGCTATTCT CTTGCAAAGA TAACTAAGCA CCAGGCCAGT AATGGGATCC
 3421 TCAGACTGGG CCCAGAAAAC CACTCTAGGG AAGTTCAGGG TAGGCTCTCT GCACCCCCTC
 3481 CTCCTAATCC CGTCTCCTTA GTGTCTTTCC GCCAGCACAG GAATGGGGGA GGGGTGGGTG
 3541 ACGAGGATGA ACACCGGAGT CCCTGGAGGA AGGGAAGCAG GGTATCTCCA TCTGAGGCTC
 3601 TGTCTTTGAG GAGAGGTGGA GAGCTGGGGA AGTCTTGTGT GAGGGGATTG GGGCTCAGGA
 3661 GGGGGTTGGG GAGCAGGAAG TTGTCCCCAG GGGAGCCATC CTGGCCCATT CAAGGGTTGA
 3721 GTACTTGTTT AGGGTTAGAG CTGCCCCCTC TGGGGACCAG GATTGTCCAG CCAAGGCCAT
 3781 TGTCCTGCCC CCTTCCCCCA GTCCCTCCCA GGCCCCTTTG AACCTGAAGT CAGATATTTC
 3841 TTCTCTCTAC CCACCTCCCA CCCGTTGGGT TTCTCCACCC AGGAACTAGG CTGGAAGCCT
 3901 GGGATGAGGA GGTGGGGGGA GGGAGAACTG AGAATCTTGA GGAAAGAGGC CCCGGCCTTA
 3961 ACTGTGAGGG GATGGAGCCT GGGTGCAGGT CTTATGGGGG TTGGGgcGGG TGGTTAGTGT
 4021 CTAATCTACC AACCTGGACA ACACAAGATG GAATACTGTG CTCTGAAAAC GCAGAGCCAG
 4081 CACTTCTCTG GGGTCTCTGG GGACATATCT GGTTGGGGCT CGGGGTCCCA TGGTGTAGAG
 4141 CCTCTAAACT CTGGAGGACT GGAGGTGCAA TGGCTGTCTT GTCCTGGCCT TGGACATGGG
 4201 CTGAAATACT GGGTTCACCC ATATCTAGGA CTCTAGACGG GTGGGTAAGC AAGAACTGAG
 4261 GAGTGGCCCC AGAAATAATT GGCACACGAA CATTCAATGG ATGTTTTAGG CTCTCCAGAG
 4321 GATGGCTGAG TGGGCTGTAA GGACAGGCCG AGAGGGTGCA GTGCCAACAG GCTTTGTGGT
 4381 GCGATGGGGC ATCCGAGCAA CTGGTTTGTG AGGTGTCCGG TGACCCAAGG CAGGGGTGAG
 4441 AGGACCTTGA AGGTTGAAAA TGAAGGCCTC CTGGGGTCCC GTCCTAAGGG TTGTCCTGTC
 4501 CAGACGTCCC CAACCTCCGT CTGGAAGACA CAGGCAGATA GCGCTCGCCT CAGTTTCTCC
 4561 CACCCCCACA GCTCTGCTCC TCCACCCACC CAGGGGGCGG GGCCAGAGGT CAAGGCTAGA
 4621 GGGTGGGATT GGGGAGGGAG AGGTGAAACC GTCCCTAGGT GAGCCGTCTT TCCACCAGGC
 4681 CCCCGGCTCG GGGTGCCCAC CTTCCCCATG ACGCGAATTC TGCAGTCGAC GGTACCGCGG
 4741 GCCCGGGATC CACCGGTCGC CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG
 4801 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC
 4861 GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC
 4921 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC
 4981 AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC
 5041 TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG
 5101 GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG
 5161 GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT
 5221 ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC
 5281 GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC
 5341 CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC
 5401 AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC
 5461 GGCATGGACG AGCTGTACAA GTAAAGCGAC TCTAGAACTA GTGGATCCCC CGGGCTGCAG
 5521 GAATTCGATA TCAAGCTGGG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC
 5581 TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAAAGAAGAC
 5641 AAGATCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC
 5701 TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC
 5761 AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT
 5821 AGTCAGTGTG GAAAATCTCT AGCAGAATTC TAGCTTGGCG TAATCATGGT CATAGCTGTT
 5881 CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG
 5941 TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG
 6001 CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG
 6061 GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC
 6121 TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC
 6181 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG
 6241 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT
 6301 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG
 6361 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA
 6421 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG
 6481 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT
 6541 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC
 6601 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC
 6661 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT
 6721 GGTATCTGCG CTCTGCTGAA GCCACTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC
 6781 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC
 6841 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
 6901 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
 6961 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
 7021 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
 7081 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
 7141 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA
 7201 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
 7261 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
 7321 TTGCGCAACG TTGTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG
 7381 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA
 7441 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT
 7501 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT
 7561 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC
 7621 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA
 7681 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT
 7741 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT
 7801 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA
 7861 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT
 7921 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA
 7981 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGG GACTAGCTTT TTGCAAAAGC
 8041 CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT
 8101 CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG
 8161 GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA
 8221 GATGTAGCTT GCATGCCGAC ATGGATTATT GACTAGTCCC TAAGAAACCA TTATTATCAT
 8281 GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTC
//