pBBR1MCS

价格:1500元

联系方式:I47-825O-882O

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pBBRMCS1载体基本信息

出品公司: --
载体名称: pBBR1MCS, pBBRMCS1, pBBRMCS, pBBR1MCS-1, pBBRMCS-1
质粒类型: 广宿主蛋白表达或克隆载体质粒,广宿主载体质粒
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: --
载体大小: 4.7 kb
5' 测序引物及序列: M13-F: TGTAAAACGACGGCCAGT
3' 测序引物及序列: M13-R: CAGGAAACAGCTATGAC
载体标签: --
载体抗性: 氯霉素
筛选标记: --
克隆菌株: DH5α 等
宿主细胞(系): 多种微生物物种,是广宿主质粒。
备注: 参考文献:
Kovach ME, Phillips RW, Elzer PH, Roop RM, 2nd, Peterson KM. pBBR1MCS: a broad-host-range cloning vector. BioTechniques 1994;16:800-2.

GenBank sequence:XXU02374
产品目录号: --
稳定性/瞬表达: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pBBRMCS1载体质粒图谱和多克隆位点信息

pBBR1MCS载体图谱
pBBR1MCS载体图谱

pBBRMCS1载体简介

pBBRMCS1载体序列

LOCUS       pBBR1MCS	4707 bp 	DNA  circular	SYN
DEFINITION  pBBR1MCS
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="pBBR1MCS"
                     /mol_type="other DNA"
     CDS             complement(1697..2212)
                     /label="ORF frame 3"
     CDS             1781..2455
                     /label="ORF frame 2"
     gene            complement(2915..4234)
                     /label="CAT/CamR (variant)"
                     /gene="CAT/CamR (variant)"
     misc_feature    complement(3033..3176)
                     /label="lacZ_a"
     misc_feature    3147..3169
                     /label="M13_pUC_fwd_primer"
     promoter        3162..3178
                     /label="M13_forward20_primer"
     promoter        3188..3206
                     /label="T7_promoter"
     misc_feature    3239..3255
                     /label="pBluescriptSK_primer"
     misc_feature    complement(3290..3306)
                     /label="pBluescriptKS_primer"
     promoter        complement(3334..3353)
                     /label="T3_promoter"
     promoter        complement(3370..3388)
                     /label="M13_reverse_primer"
     misc_feature    complement(3387..3409)
                     /label="M13_pUC_rev_primer"
     promoter        complement(3423..3452)
                     /label="lac_promoter"
     CDS             complement(3545..4234)
                     /label="ORF frame 3"
     CDS             complement(4487..1192)
                     /label="ORF frame 3"
ORIGIN
    1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
   61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
  121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
  181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
  241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
  301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
  361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
  421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
  481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
  541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
  601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
  661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
  721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
  781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
  841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
  901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
  961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
 1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
 1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
 1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
 1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
 1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
 1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
 1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
 1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
 1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
 1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
 1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
 1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
 1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
 1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
 1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
 1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
 1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
 2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
 2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
 2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
 2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
 2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
 2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
 2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
 2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
 2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
 2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
 2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
 2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
 2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
 2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
 2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
 2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
 3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
 3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
 3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
 3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
 3241 tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga
 3301 cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
 3361 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
 3421 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
 3481 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
 3541 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcata
 3601 aaaactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
 3661 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
 3721 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
 3781 tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg
 3841 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
 3901 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
 3961 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt
 4021 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
 4081 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
 4141 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
 4201 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
 4261 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
 4321 ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat
 4381 caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt
 4441 cgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
 4501 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcgcccgcgt tcctgctggc
 4561 gctgggcctg tttctggcgc tggacttccc gctgttccgt cagcagcttt tcgcccacgg
 4621 ccttgatgat cgcggcggcc ttggcctgca tatcccgatt caacggcccc agggcgtcca
 4681 gaacgggctt caggcgctcc cgaaggt
//

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pBBRMCS1载体应用举例

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