| 质粒名称: | Pveg |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 55173 |
| 存储实验室: | Thorsten Mascher |
|
载体骨架基本信息 |
|
| 载体名称: | pSB1C3 |
| 载体出品公司: | iGEM |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 2070 bp |
| 完整质粒大小: | 2307bp |
| 载体修饰: | -- |
| 载体类型: | -- |
| 筛选标记: | 氯霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | veg promoter |
| 物种来源: | -- |
| 插入大小: | 237 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | Pveg was a gift from Thorsten Mascher (Addgene plasmid # 55173 ; http://n2t.net/addgene:55173 ; RRID:Addgene_55173) |
| 参考文献部分: | The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29. 10.1186/1754-1611-7-29 PubMed 24295448 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Pveg 2307 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Very strong constitutive promoter.
ACCESSION .
VERSION .
KEYWORDS Pveg
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2307)
AUTHORS Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J,
Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
TITLE The Bacillus BioBrick Box: generation and evaluation of essential
genetic building blocks for standardized work with Bacillus
subtilis.
JOURNAL J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
PUBMED 24295448
REFERENCE 2 (bases 1 to 2307)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..2307
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 2..59
/label=his operon terminator
/note="This putative transcriptin terminator from the E.
coli his operon has a 2-bp deletion introduced during
synthesis. Its efficiency has not been determined."
primer_bind complement(83..100)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(254..842)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(334..353)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
terminator 1025..1119
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(1140..1799)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 1714..1733
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
primer_bind 1961..1979
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
terminator 1983..2026
/label=bacterial terminator
/note="putative bacterial transcription terminator"
misc_feature 2029..2050
/label=BioBrick prefix
/note="BioBrick prefix for parts that do not start with
""ATG"""
misc_feature 2288..1
/label=BioBrick suffix
/note="universal suffix for all parts"
ORIGIN
1 gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
61 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
121 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
181 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
241 cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct
301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
661 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
961 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc
1021 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
1081 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
1141 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
1201 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
1261 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
1321 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
1381 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
1441 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
1501 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
1561 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
1621 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
1681 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
1741 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
1801 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
1861 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
1921 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
1981 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc
2041 gcttctagag ggagttctga gaattggtat gccttataag tccaattaac agttgaaaac
2101 ctgcatagga gagctatgcg ggttttttat tttacataat gatacataat ttaccgaaac
2161 ttgcggaaca taattgagga atcatagaat tttgtcaaaa taattttatt gacaacgtct
2221 tattaacgtt gatataattt aaattttatt tgacaaaaat gggctcgtgt tgtacaataa
2281 atgtagttac tagtagcggc cgctgca
//
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