| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pXINSECT-DEST39 |
| 质粒类型: | 昆虫表达载体；Gateway载体 |
| 克隆方法: | Gateway |
| 启动子: | Actin |
| 载体大小: | 12447 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | 6XHis tag （N-末端） |
| 载体抗性: | 氨苄青霉素、氯霉素（仅空载体） |
| 真核筛选标记: | 无 |
| 克隆菌株: | DB3.1 |
| 宿主细胞（系）: | High Five（推荐） 等细胞系 |
| 备注: |
pXINSECT-DEST39载体与pBmA-neo载体共转染，可以建立稳表达细胞系； 应用Gateway技术，可快速高效将 目的基因片段（尤其适用于目的基因片段>1kb，用传统的连接酶法效率低的）插入到载体中； |
| 产品目录号: | 12249-025 |
| 瞬表达/稳表达: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
pXINSCET-DEST39载体优势：
Using the Express Insect Kit or the Express Insect Vector Set to achieve protein expression in insect cells has the following advantages:
Non-lytic, continuous protein expression
Minimal degradation of your protein of interest as compared to viral expression systems
Higher protein expression level achieved than other similar non-viral insect expression systems
Choice of transient or stable protein expression
Adapted for use with the Gateway Technology or traditional restriction enzyme-mediated cloning
pXINSCET-DEST39载体特征：
The silkmoth (Bombyx mori) cytoplasmic actin gene promoter for constitutive expression of the gene of interest (Johnson et al., 1992)
The viral transcriptional enhancer, homologous region 3 (HR3) from Bombyx mori nuclear polyhedrosis virus (BmNPV) acts as a constitutive enhancer of the cytoplasmic actin promoter (Lu et al., 1997)
A viral transcriptional transactivator, BmNPV IE1, to stimulate expression of your gene of interest (Lu et al., 1996)
N-terminal 6xHis tag fusion proteins to facilitate detection and purification of your protein of interest using pXINSECT-DEST39
Two recombination sites, attR1 and attR2, for recombinational cloning of the gene of interest from an entry clone
Chloramphenicol resistance gene located between the two attR sites for counterselection
The ccdB gene located between the two attR sites for negative selection
The actin polyadenylation signal for efficient transcription termination and polyadenylation of mRNA
Ampicillin resistance gene for selection of transformants in E. coli
The pUC origin for high copy replication and maintenance of the plasmid in E. coli
载体序列
GAGGTCGACGGTATCGATTGCAGGTCGATATTTAAAAAAAATTATAATAATGTTAAAGTTGCTTCATACG TTGAAGTACCTTACACAACAAATAATGCAGACGTCGAAATGACTGACATAACAAATGCGCCCTTTCGCCC AAAATCTAAAGCAAGGAGAAGATTAGATTTTACCAACATGGCGCCGCAGCCGTGTTGTATTGACGACGGC AATTTTGCAAAACTTTACTGTGATATATTTATTAAATTAAGTTTGCTTTAAAATGAGTTTTTTTACAAAT CTTCGCAGAGTCAATAAATTGTATCCTAATCAGGCCAGTTTTCTTGCTGATAATACGCGTCTTTTAACAA GGCACTCCCGCCGGTTTCACAAATGTGCTCAACGCGCCCAGTGTACGCAACCTTGGAAACAACAGATATG GGCCGGGCTATCAATTATCTAACAACCGGTTTGTGAGCACTTCAGACATAAACAGAATTACTCGTAACAA CGATGTCCCCAACATACGCGGAGTATTTCAGGGCATTTCAGACCCTCAAATAAACTCATTGAGCCAATTG CGGCGCATGGACCAACGTGCCAGACTTTCATTACCACACCAAACAGACGCGATCCAATGCAGTCAGACAA AACTTCCCGGAGACCAACGTGCGCACGCCCGAAGGTGTTCAAAATGCACTGCAGCAAAACCCCCCCGTTT ACATAATCACATGAGAACCTTGAAAGTAGCAGGAGTGGGCATACTCTTGGGCCGGCGGCGGTTATCTTTT GTTTACCGCCGCCACATTAGTACAAGATATAATCAACGCCATCAATAGAACCGGCGGAAGTTATTATGTG CAAGGTAGAAACGCCGGAGAAAACGCCGAGGCCTGTTTGTTATTGCAGCGCACTTGTCGTCAAGACCGCA ATCTAGCTCAGTCGGATGTTAACATTTGCTCAAGAGACCCCTTGTTGGCTAACGATTCGCCCCTACTAAC CAACATGTGCCAAGGATTTAACTATGAAACAGAAAAAACAGTTTGTCGCGGCAGCAATCCGGCCGCTAAC CCAACTTCGCCTCAATACGTAGATATTAGCGATCTTCTGCGGGCCAAACAATCATGTGCATCGAACCTTA CACGTTTAGTGATTTAATTGGCGACTTGCGTTTACATTGGTTACTGGGAAGAGAAGGTTTAATCGGCAAA TCGTCCAACGGTAGTGACAGCATCCGCAACAAAATAATGCCTCATCATTATGATGATAGGCGCGTTCTTG TTTTTAGGTTTAATACTTTATTTTATCTACAGATACATGACAAAAGGAGGAGGAGGAGGAGGAAGCGGTG GGGCACCAACTCCCATTGTTGTTATTATGCAACACCCCACATCAACAGCGGCCCCTCGTCGATAATAAAA GACAAAAATAATATAAAATATATGTATAATTAATTAAATTCAAAAGATATGTATAATTAATTAAATTCAA ATTTTTTATATTTACAATTTAGTTTTTGTTCCGCAAACGTTATAGCGTCGGACAACGGAACCAGACCCTG TAATATTAAAGCTAACAATTTTAACAAATTATTGTGCAATGTAGTGCTCTCTCTTCGGTTCACTTTACTG ATTACAAACATGTGATGCTTAAATCTATTATATTTTTGAATTACTTGACTAGCGTCTACATCTTTAATCT CGCCAGAAATCCAATAAAACTCTTCGTTTTTCTTAGCTATAGTCAACCGCTCTTCGTTTTTGAAAGACAA TACTATAAAATTGTGACCTTTTACATTATCCACATTCTGAGTCAAATACTGTTCGACAATGTGCATGCTG CCGTCCTCCTTCTTAACCTTTTTTAAATTTTCAGCGTTATTATTACTCGCAATATTGTCATGATATTTAT AATTATTAAACAAAAGATTAGCGACACTACTGTATTTGTACGTGAGCGTACTTTTTTTGTTAACAATTAA ATTTAAATTGTCCACCACATATTTGTTTGGGGGATTGTCGGGAAACTTTACACTTTCCGAATACTTTAAT ATTTGACTCACATACGGCGATACAAAAAAATTATTAGATGCAGTCTCAATTTCATTACTCTCTTTACGAC TAAGCATAATAGGCAAAGTAAATAAATTTTTATCTTGATACATTTCGTACAACTTGCTCAAAAGAAACCC ACACTTTCTTTCGCCCAACGATTGTAACAAAGTCACAAATGTGGTTTGCGCGTAATACATATCTAAATTA AAATATGAAGTCAGAGCAGCTTTAAACGTGTGATGCACATCGACAAAGTGGCATTTTTTACAATTTTGTG CAGCCGTCTCGTCGTTGCACACATCTTGAGAATGAGGAATTTCTATGCCGGTTTCTTTAACCAAATTGTA CGAGATCATAAATCTAATTTTATCAAAAGTTACCACAAACACGCGATTATCTACCATGTAATAGTTGTTT GTATATTCGTACACCACATTGCTCACGTACTTGGCAAATATAATTTCAAACGGCTTTACTTCACTTTTTT TAACCACAAACATGTAATAACCAGTTTCGGACATATGGTCGGAGAACCTATTGGAATTGTAGTCGTTGTC GTCGAAACGCATCAAATACGGCGCAAAATCATTAGTAAAATAATGCGTAATTTCTTGAGTTGAAGCAACC GTGCAAATGTTCGTGTTGTGATTAATTGTCTGCTCAAGGGTTGCACAGCTTTGAATTGTGCTTTTCTTGT ATTTAGGCTTCAATTTATTCTTGTTAAATTGGCCCACCACACTTTGTGAATCGTCCAAGTATTCGTCCAG CTTCCGTTTAGTTCCAGTTGCCGATGGTTGGTTCACACCAACAGGATGCTCAAAAGATTCCGCATTATAA GCAGAACTGGGCGATGGTTGCTCCGCAACAGGCAGCTCAAAAGATTCCGCATTATAAGCAGAACTAACTG CTTCTCCGAGATTATCAGTGGTCTTGAGCAAACATTCCATTATATCGTTATCATCAGTTAACGAATTGAC GCTTGCCAAAAAGTTTGAAGCTGCCTGCAGTCTGCTGTCAGATACTACCGTGTCGGCTCCATCCGGCGTG GGATTGTTATAATAATTCAAATAGTCGTTGGGCTGTTGTTTATCACAAAACTCTGAATAGCCGTTGTCGA ACGACGCTCGGGACGGCGTCGGAGCACTGGTGTACGACGCGTTAAAATTAATTTGCGTCATAGTCGTTTG GTTGTTCACGATCGTGTCCCGCCAATGTCAACTTGCAACTGAAACAATATTCAACATGAACGTCAATTTA TACTGCCCTAATGGCGAACACGATAATAATATTTTTTTTATTATGCCCTCTAAAACCAATGCGGTTATCG TTTATTTATTCAAATTAGATACAGAACATCCGCCGACATACAATGTTAATGCAAAAACTCGTTTGGTGAG CGGATACGAAAACAGTCGGCCGATAAACATTAATCTGAGGTCGATAACACCGTCCTTGAACGGAACACGA GGAGCGTACGTGATCAGCTGCATTCGCGCGCCGCGCCTTTATCGAGATTTATTTACATACAACAAGTACA CTGCGCCGTTGGCATTTGTGGTAACGCGCACACAAGCAGAGCTGCAAGTGTGGCACATTTTGTCTGTGCG CAAAACCTTTGAAGCCAAAAGCACAAGGTCCGTTACGGGCATGCTAGCGCACACGGACAACGGACCCGAC AAATTCTACGCCAAGGATTTAATGATAATGTCGGGCAACGTGTCGGTGCATTTTATTAATAACTTACAAA ATGTCGCGCGCATCACAAAGACATTGATATATTTAAACATTTATGTCCCGAACTGCAAATCGATCGATAA GCTTGATATCGAATTCCTGCAGCCCAATATTACGTTCGTGCCAGAAATTAATTTCTCCGCGTCGTATTAT ACGATTTATACGGTACAGCAGCTTGGCCCACAAATAGATCGTTTTATGATTTTGATGATGGAGGTGCGCT CAAGATGAAACCCATTCAGACGTTATTAGTTGCGTCAAGTATTTGGCAATTTGCTACGACGCAATTATTG TGGAAGAAGCGTAATTTGTGAACAGCCCATTCGAGGCTAGATTGAAAAAGTATATTGATATTAAATCATA TAAATTGTTTATGAGGCCTTCAAACGAATCTTGTAAAGATTATTTATTAAAATTGTTCAACGATTGTATG AGAGGGTCATTTGTTTTTCAAAACTGAACTCGCTTTACGAGTAGAATTCTACTTGTAAAACACAATCAAG AGATGATGTCATTTGTTTTTCAAAACTGAATGATGTCATTTGTTTTTTAAAACTAAACTCGCTTTTACGA GTAGAATTCTACGTGTAAAACATAATCAAGAGATGATGTCATTTGTTTTTCAAAACTGAACCGGCTTTAC GAGTAGAATTCTACGTGTAAAACATAATCAAGAGATGATGTCATCATTAAACTGATGTCATTTTTATACA CGATTGTTAACATGTTTAATAATGACTAATTTGTTTTTCAAATTAAACTCGCTTTACAAGTAGAATTCTA CTTGTAACGCACGATTAAAATTATTATAATCAGGAATGATGTCATTTGTTTTCGTCATAAAATGTTTATA CAACGGAATCTTCTTGTAAATTATCCAAATAATATAATTTATCCGATTCTACGTTACATTTAAATTCGTT GTTATCGTACAATTCTTCAGGACACGCCATGTATTGGCCGTTTTTAACGTGCAACCAACGATTGTATTTG ACGCCGTCGTTGGATTGCGTGTTCAGGTTGGCGTACACGTGACTGGGCACGGCTTCTTTTTTTACCACTA TCGCATCTTCGTCGTACGCGGATCTACAACCAATCCCGTTGCCCACATAAGCGTACGCGTTTAAAACGTG CGATAGGTCTTTGGCCAATTCGCAATCAGCGTCCACTTTAACGTTGTTGCGTAACTCGTTTAAAGCATTA ATAATGACGTCATTTTCCGCATGACAACTGGTTAGCTTGAAAAACGGAACCGAGTAGTGGCATGAATAAA ATAAATCTTTGTTGTCTAATATTGGGGGGGAGCTCTTGTGAGTCCTCGCGGGTAGGTACCACCACCCTGC CTATTTCTGCCGTGAAGCAGTAATGCGTTTCGGTTTGAAGAGTGGGGCGGCCGTGGTACTGAGACCTTAG AACTCATATCTGAAGGTGGGTGGCACATTTACGTTGTAGATGTCTATGGGCTCCAGTAACCACTTAACAT CAGGTGGGCTGTGAGCTCTTACACCCATCTACGCAATAAAAAATTAAAAATAAATATGTTTGAAGTCCGT AACATAGATTCCGTATTTTTACAGTTGTTTTTCACGTTTTTCATTTCTTCACCGACAATGGAAAATAATC ACACACAAATACACTGTATAGTAACAACGAGCAGAGCCGATTTTGGAGTTTCGATAAAGCGAGGCTACCA AGAATGCGGCAGATAAGATTTACGTACATTCAAGAGTCGCTGATAACAACTTTTACCTCTCAAATTGCCC ACAGTGCGATCACAAGAAACATAGACGAACGGATCTGTGCGCAACGAGCCGCTACGATATCATTATCATA CAGATTTTTATCTTTTCATCTAGCTTCAGTTAGTGATGCTTTCTGATCTCTTCATAATTATAATTAAAAA GAATAAATTATCTAGTAATATAGTTCTACTACGGTACACGAATTTTGAGATTAATTAACCGGATTTTCTG GGTTATGATTTACATCGGTACAGAATCTAGTGAAAGCACGTCGAGTGAAATTCTATGAAACTTCGGCGGG AGTCGGGGAGAGGTTACAAGCGACCGCGAGGTGCCGCTAACTTAATCAGTTATCAAGGCATCGCCTTATC AAAAGATGCGAGCTGATAGCGTGCGCGTTACCATATATGGTGACAAAAACTGAGTCAGCCCGCGATTGGT GGAAAAACAAACTGGAGCCGATACTGTGTAAATTGTGATAACGGCTCTTTTATATAGTTTATCCTCACGA GTCGGTTCTCATTTACTAAGGTGTGCTCGAACAGTGCGCATTCGCATCTACGTACTTGTCACTTATTTAA TAATACTATGTAAGTTTTAATTTTAAAATTGCGAAAGAAAAAAAAACATATTTATTTATTTGTAAAATTT GAATTTCGAAGGTTCTCCGTCCCTTTACCTTTAAGTATTACATATGTTTGAGTGTTTTTTTTTTTTAATA ATACGCTAATGATAACGTGTTACGTTACATAATTGTTGCATAACTAGTGAAGTGAAATTTTTTATAAAAA AAAACATTTTTCGGAATTTAGTGTACTGCAGATGTTAATAAACACTACTAAATAAGAAATAAGTTTATTG GACGCACATTTCAAAGTGTCCACTCGCATCGATCAATTCGGAAACAGAAATTGGGAACAGTGAATTATGA ATCTTATACAGTTTTCTTTAACGTCACTAAATAGATGGACGCAAATAAATTTGTCGTTTACTTAGTATAA TGTATGGAATGAGAATGTAGTTTGAATTGTTTTTTTTCTTTTCTTGCAGACTAATTCAAGAGGTGCGACG AAGAAGTTGCCGCGTTGGTAGTAGACGGTATCGATAAGCTTGATATCGAATTCCTGCAGCCCAGCATATG CGCGGTTCGCATCACCATCACCATCACAGCGCTGGATCCTCGATCACAAGTTTGTACAAAAAAGCTGAAC GAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATA CTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTT CCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAAT GGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCA TTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCG TAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCC GGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTT TTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTAC ACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAA TATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGAC AACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGG CGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTA CTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCG TATTTGCGCGCACCGGTGCTAGCGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTAT TACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGG TAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGG GATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAAAT GCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATT ATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCC GTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGT GCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATT AACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCGTTATACACAGCCAGTCTGCAGGTCGACCATAGT GACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGA TATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATAATTAATTAAGATCGAAT CTAGAGCGGCCGCCACCGCCTCGAGAAGTCTTACGAACTTCCCGACGGTCAGGTCATCACCATCGGAAAC GAAAGATTCCGTTGCCCAGAGGCCCTCTTCCAACCCTCGTTCTTGGGTATGGAAGCCAACGGAATCCACG AAACCACATACAACTCCATCATGAAGTGCGACGTGGACATCCGTAAGGACTTGTACGCCAACACCGTATT GTCCGGTGGTACCACCATGTACCCTGGAATCGCCGACCGTATGCAAAAGGAAATCACACGTCTCGCCCCA TCGACAATGAAGATTAAGATCATCGCTCCCCCAGAGAGGAAGTACTCCGTATGGATCGGTGGATCGATCC TCGCCTCCCTCTCTACCTTCCAACAGATGTGGATCTCGAAACAGGAGTACGACGAGTCTGGTCCCTCCAT TGTACACAGGAAGTGCTTCTAAGCGTTGAGACTTTAAGTTATGATGCCCTACAGCAGAACCTCAAGAGGG TGGCTCAAATTACGCTTGTGATCTTGTAAATAAATTCAGTATTTAATGTAGGTTGTAAGGTATTGTAATA TGCATATTACGTAAAACGAACGGAATGTTGTTGTTGCCGTTTTTTTTTTGACAAAGATTTTTATTTATTA AAGTTACTAACCCCAAAACTTTTTAATAAAATAAATTTATATACCGGTATAATAACTGACGTTTTTCACT TGCTGTCCCCGCTCCCGACTAACAGTACGTCGTGTGCACCGAAATTACCGATTTCGTACACCGTTTGAGA CAGTTACGCTAGGAGCACAAATCTCCCAGCTGCATACCGTTGTTTACTGCAGCTGCAGTCTTTAATTGGA ATGCGAGTCGTTGACCGCTTAATACGAAACATTCTAAAATTCGCAAAATGCAAAGGAAACTGGTTCTGTA CTTTCTACCTTTCAAAAGATTCACCAAATTAATTTTATGCGGACTCACTAATTCCGTAGAAATCTGTGTA GAGGTACCCAGGTTACGCTTAGGCATAAGATGACTGTTCGCGTTTTTACAATACATACGAGCAGGTTACA CACAAGATGAACATCCTTTGATGCGTCTGTGTCTTGACCCGTCTGAGATTTGAGTGACTTGTCAACGTCA TTGCGTAGTGTCACCGGTCGTCGAGATCCCCGCCGCGGTGGAGCTACGAGCTCCAGCTTTTGTTCCCTTT AGTGAGGGTTAATTCCGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCT CACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAA CTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAAT GAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTC GCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACA GAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCA GAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCT CCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACC CCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGAC TTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCC AGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGG GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTT CACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCT GACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTG CCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGAT ACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGC AGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTA GTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTT TGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAA AAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGG TTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTA CTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGAT AATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCT CAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATC TTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGG GCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATT GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCC CCGAAAAGTGCCACCTGGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAA TCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGAT AGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGG CGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGA GGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGC GAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTC ACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCAT TCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGG GGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACG GCCAGTGAATTGTAATACGACTCACTATAGGGCGAATTGGGTACCGGGCCCCCCCTC
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