pXINSECT-DEST39

价格:3000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Invitrogen
载体名称: pXINSECT-DEST39
质粒类型: 昆虫表达载体;Gateway载体
克隆方法: Gateway
启动子: Actin
载体大小: 12447 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: 6XHis tag (N-末端)
载体抗性: 氨苄青霉素、氯霉素(仅空载体)
真核筛选标记:
克隆菌株: DB3.1
宿主细胞(系): High Five(推荐) 等细胞系
备注: pXINSECT-DEST39载体与pBmA-neo载体共转染,可以建立稳表达细胞系;
应用Gateway技术,可快速高效将 目的基因片段(尤其适用于目的基因片段>1kb,用传统的连接酶法效率低的)插入到载体中;
产品目录号: 12249-025
瞬表达/稳表达: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pXINSECT-DEST39 载体图谱



pXINSECT-DEST39 多克隆位点

pXINSECT-DEST39 载体特征

载体简介

pXINSCET-DEST39载体优势:

Using the Express Insect Kit or the Express Insect Vector Set to achieve protein expression in insect cells has the following advantages:
 Non-lytic, continuous protein expression
 Minimal degradation of your protein of interest as compared to viral expression systems
 Higher protein expression level achieved than other similar non-viral insect expression systems
 Choice of transient or stable protein expression
 Adapted for use with the Gateway Technology or traditional restriction enzyme-mediated cloning

pXINSCET-DEST39载体特征:

 The silkmoth (Bombyx mori) cytoplasmic actin gene promoter for constitutive expression of the gene of interest (Johnson et al., 1992)
 The viral transcriptional enhancer, homologous region 3 (HR3) from Bombyx mori nuclear polyhedrosis virus (BmNPV) acts as a constitutive enhancer of the cytoplasmic actin promoter (Lu et al., 1997)
 A viral transcriptional transactivator, BmNPV IE1, to stimulate expression of your gene of interest (Lu et al., 1996)
 N-terminal 6xHis tag fusion proteins to facilitate detection and purification of your protein of interest using pXINSECT-DEST39
 Two recombination sites, attR1 and attR2, for recombinational cloning of the gene of interest from an entry clone
 Chloramphenicol resistance gene located between the two attR sites for counterselection
 The ccdB gene located between the two attR sites for negative selection
 The actin polyadenylation signal for efficient transcription termination and polyadenylation of mRNA
 Ampicillin resistance gene for selection of transformants in E. coli
 The pUC origin for high copy replication and maintenance of the plasmid in E. coli

载体序列

GAGGTCGACGGTATCGATTGCAGGTCGATATTTAAAAAAAATTATAATAATGTTAAAGTTGCTTCATACG
TTGAAGTACCTTACACAACAAATAATGCAGACGTCGAAATGACTGACATAACAAATGCGCCCTTTCGCCC
AAAATCTAAAGCAAGGAGAAGATTAGATTTTACCAACATGGCGCCGCAGCCGTGTTGTATTGACGACGGC
AATTTTGCAAAACTTTACTGTGATATATTTATTAAATTAAGTTTGCTTTAAAATGAGTTTTTTTACAAAT
CTTCGCAGAGTCAATAAATTGTATCCTAATCAGGCCAGTTTTCTTGCTGATAATACGCGTCTTTTAACAA
GGCACTCCCGCCGGTTTCACAAATGTGCTCAACGCGCCCAGTGTACGCAACCTTGGAAACAACAGATATG
GGCCGGGCTATCAATTATCTAACAACCGGTTTGTGAGCACTTCAGACATAAACAGAATTACTCGTAACAA
CGATGTCCCCAACATACGCGGAGTATTTCAGGGCATTTCAGACCCTCAAATAAACTCATTGAGCCAATTG
CGGCGCATGGACCAACGTGCCAGACTTTCATTACCACACCAAACAGACGCGATCCAATGCAGTCAGACAA
AACTTCCCGGAGACCAACGTGCGCACGCCCGAAGGTGTTCAAAATGCACTGCAGCAAAACCCCCCCGTTT
ACATAATCACATGAGAACCTTGAAAGTAGCAGGAGTGGGCATACTCTTGGGCCGGCGGCGGTTATCTTTT
GTTTACCGCCGCCACATTAGTACAAGATATAATCAACGCCATCAATAGAACCGGCGGAAGTTATTATGTG
CAAGGTAGAAACGCCGGAGAAAACGCCGAGGCCTGTTTGTTATTGCAGCGCACTTGTCGTCAAGACCGCA
ATCTAGCTCAGTCGGATGTTAACATTTGCTCAAGAGACCCCTTGTTGGCTAACGATTCGCCCCTACTAAC
CAACATGTGCCAAGGATTTAACTATGAAACAGAAAAAACAGTTTGTCGCGGCAGCAATCCGGCCGCTAAC
CCAACTTCGCCTCAATACGTAGATATTAGCGATCTTCTGCGGGCCAAACAATCATGTGCATCGAACCTTA
CACGTTTAGTGATTTAATTGGCGACTTGCGTTTACATTGGTTACTGGGAAGAGAAGGTTTAATCGGCAAA
TCGTCCAACGGTAGTGACAGCATCCGCAACAAAATAATGCCTCATCATTATGATGATAGGCGCGTTCTTG
TTTTTAGGTTTAATACTTTATTTTATCTACAGATACATGACAAAAGGAGGAGGAGGAGGAGGAAGCGGTG
GGGCACCAACTCCCATTGTTGTTATTATGCAACACCCCACATCAACAGCGGCCCCTCGTCGATAATAAAA
GACAAAAATAATATAAAATATATGTATAATTAATTAAATTCAAAAGATATGTATAATTAATTAAATTCAA
ATTTTTTATATTTACAATTTAGTTTTTGTTCCGCAAACGTTATAGCGTCGGACAACGGAACCAGACCCTG
TAATATTAAAGCTAACAATTTTAACAAATTATTGTGCAATGTAGTGCTCTCTCTTCGGTTCACTTTACTG
ATTACAAACATGTGATGCTTAAATCTATTATATTTTTGAATTACTTGACTAGCGTCTACATCTTTAATCT
CGCCAGAAATCCAATAAAACTCTTCGTTTTTCTTAGCTATAGTCAACCGCTCTTCGTTTTTGAAAGACAA
TACTATAAAATTGTGACCTTTTACATTATCCACATTCTGAGTCAAATACTGTTCGACAATGTGCATGCTG
CCGTCCTCCTTCTTAACCTTTTTTAAATTTTCAGCGTTATTATTACTCGCAATATTGTCATGATATTTAT
AATTATTAAACAAAAGATTAGCGACACTACTGTATTTGTACGTGAGCGTACTTTTTTTGTTAACAATTAA
ATTTAAATTGTCCACCACATATTTGTTTGGGGGATTGTCGGGAAACTTTACACTTTCCGAATACTTTAAT
ATTTGACTCACATACGGCGATACAAAAAAATTATTAGATGCAGTCTCAATTTCATTACTCTCTTTACGAC
TAAGCATAATAGGCAAAGTAAATAAATTTTTATCTTGATACATTTCGTACAACTTGCTCAAAAGAAACCC
ACACTTTCTTTCGCCCAACGATTGTAACAAAGTCACAAATGTGGTTTGCGCGTAATACATATCTAAATTA
AAATATGAAGTCAGAGCAGCTTTAAACGTGTGATGCACATCGACAAAGTGGCATTTTTTACAATTTTGTG
CAGCCGTCTCGTCGTTGCACACATCTTGAGAATGAGGAATTTCTATGCCGGTTTCTTTAACCAAATTGTA
CGAGATCATAAATCTAATTTTATCAAAAGTTACCACAAACACGCGATTATCTACCATGTAATAGTTGTTT
GTATATTCGTACACCACATTGCTCACGTACTTGGCAAATATAATTTCAAACGGCTTTACTTCACTTTTTT
TAACCACAAACATGTAATAACCAGTTTCGGACATATGGTCGGAGAACCTATTGGAATTGTAGTCGTTGTC
GTCGAAACGCATCAAATACGGCGCAAAATCATTAGTAAAATAATGCGTAATTTCTTGAGTTGAAGCAACC
GTGCAAATGTTCGTGTTGTGATTAATTGTCTGCTCAAGGGTTGCACAGCTTTGAATTGTGCTTTTCTTGT
ATTTAGGCTTCAATTTATTCTTGTTAAATTGGCCCACCACACTTTGTGAATCGTCCAAGTATTCGTCCAG
CTTCCGTTTAGTTCCAGTTGCCGATGGTTGGTTCACACCAACAGGATGCTCAAAAGATTCCGCATTATAA
GCAGAACTGGGCGATGGTTGCTCCGCAACAGGCAGCTCAAAAGATTCCGCATTATAAGCAGAACTAACTG
CTTCTCCGAGATTATCAGTGGTCTTGAGCAAACATTCCATTATATCGTTATCATCAGTTAACGAATTGAC
GCTTGCCAAAAAGTTTGAAGCTGCCTGCAGTCTGCTGTCAGATACTACCGTGTCGGCTCCATCCGGCGTG
GGATTGTTATAATAATTCAAATAGTCGTTGGGCTGTTGTTTATCACAAAACTCTGAATAGCCGTTGTCGA
ACGACGCTCGGGACGGCGTCGGAGCACTGGTGTACGACGCGTTAAAATTAATTTGCGTCATAGTCGTTTG
GTTGTTCACGATCGTGTCCCGCCAATGTCAACTTGCAACTGAAACAATATTCAACATGAACGTCAATTTA
TACTGCCCTAATGGCGAACACGATAATAATATTTTTTTTATTATGCCCTCTAAAACCAATGCGGTTATCG
TTTATTTATTCAAATTAGATACAGAACATCCGCCGACATACAATGTTAATGCAAAAACTCGTTTGGTGAG
CGGATACGAAAACAGTCGGCCGATAAACATTAATCTGAGGTCGATAACACCGTCCTTGAACGGAACACGA
GGAGCGTACGTGATCAGCTGCATTCGCGCGCCGCGCCTTTATCGAGATTTATTTACATACAACAAGTACA
CTGCGCCGTTGGCATTTGTGGTAACGCGCACACAAGCAGAGCTGCAAGTGTGGCACATTTTGTCTGTGCG
CAAAACCTTTGAAGCCAAAAGCACAAGGTCCGTTACGGGCATGCTAGCGCACACGGACAACGGACCCGAC
AAATTCTACGCCAAGGATTTAATGATAATGTCGGGCAACGTGTCGGTGCATTTTATTAATAACTTACAAA
ATGTCGCGCGCATCACAAAGACATTGATATATTTAAACATTTATGTCCCGAACTGCAAATCGATCGATAA
GCTTGATATCGAATTCCTGCAGCCCAATATTACGTTCGTGCCAGAAATTAATTTCTCCGCGTCGTATTAT
ACGATTTATACGGTACAGCAGCTTGGCCCACAAATAGATCGTTTTATGATTTTGATGATGGAGGTGCGCT
CAAGATGAAACCCATTCAGACGTTATTAGTTGCGTCAAGTATTTGGCAATTTGCTACGACGCAATTATTG
TGGAAGAAGCGTAATTTGTGAACAGCCCATTCGAGGCTAGATTGAAAAAGTATATTGATATTAAATCATA
TAAATTGTTTATGAGGCCTTCAAACGAATCTTGTAAAGATTATTTATTAAAATTGTTCAACGATTGTATG
AGAGGGTCATTTGTTTTTCAAAACTGAACTCGCTTTACGAGTAGAATTCTACTTGTAAAACACAATCAAG
AGATGATGTCATTTGTTTTTCAAAACTGAATGATGTCATTTGTTTTTTAAAACTAAACTCGCTTTTACGA
GTAGAATTCTACGTGTAAAACATAATCAAGAGATGATGTCATTTGTTTTTCAAAACTGAACCGGCTTTAC
GAGTAGAATTCTACGTGTAAAACATAATCAAGAGATGATGTCATCATTAAACTGATGTCATTTTTATACA
CGATTGTTAACATGTTTAATAATGACTAATTTGTTTTTCAAATTAAACTCGCTTTACAAGTAGAATTCTA
CTTGTAACGCACGATTAAAATTATTATAATCAGGAATGATGTCATTTGTTTTCGTCATAAAATGTTTATA
CAACGGAATCTTCTTGTAAATTATCCAAATAATATAATTTATCCGATTCTACGTTACATTTAAATTCGTT
GTTATCGTACAATTCTTCAGGACACGCCATGTATTGGCCGTTTTTAACGTGCAACCAACGATTGTATTTG
ACGCCGTCGTTGGATTGCGTGTTCAGGTTGGCGTACACGTGACTGGGCACGGCTTCTTTTTTTACCACTA
TCGCATCTTCGTCGTACGCGGATCTACAACCAATCCCGTTGCCCACATAAGCGTACGCGTTTAAAACGTG
CGATAGGTCTTTGGCCAATTCGCAATCAGCGTCCACTTTAACGTTGTTGCGTAACTCGTTTAAAGCATTA
ATAATGACGTCATTTTCCGCATGACAACTGGTTAGCTTGAAAAACGGAACCGAGTAGTGGCATGAATAAA
ATAAATCTTTGTTGTCTAATATTGGGGGGGAGCTCTTGTGAGTCCTCGCGGGTAGGTACCACCACCCTGC
CTATTTCTGCCGTGAAGCAGTAATGCGTTTCGGTTTGAAGAGTGGGGCGGCCGTGGTACTGAGACCTTAG
AACTCATATCTGAAGGTGGGTGGCACATTTACGTTGTAGATGTCTATGGGCTCCAGTAACCACTTAACAT
CAGGTGGGCTGTGAGCTCTTACACCCATCTACGCAATAAAAAATTAAAAATAAATATGTTTGAAGTCCGT
AACATAGATTCCGTATTTTTACAGTTGTTTTTCACGTTTTTCATTTCTTCACCGACAATGGAAAATAATC
ACACACAAATACACTGTATAGTAACAACGAGCAGAGCCGATTTTGGAGTTTCGATAAAGCGAGGCTACCA
AGAATGCGGCAGATAAGATTTACGTACATTCAAGAGTCGCTGATAACAACTTTTACCTCTCAAATTGCCC
ACAGTGCGATCACAAGAAACATAGACGAACGGATCTGTGCGCAACGAGCCGCTACGATATCATTATCATA
CAGATTTTTATCTTTTCATCTAGCTTCAGTTAGTGATGCTTTCTGATCTCTTCATAATTATAATTAAAAA
GAATAAATTATCTAGTAATATAGTTCTACTACGGTACACGAATTTTGAGATTAATTAACCGGATTTTCTG
GGTTATGATTTACATCGGTACAGAATCTAGTGAAAGCACGTCGAGTGAAATTCTATGAAACTTCGGCGGG
AGTCGGGGAGAGGTTACAAGCGACCGCGAGGTGCCGCTAACTTAATCAGTTATCAAGGCATCGCCTTATC
AAAAGATGCGAGCTGATAGCGTGCGCGTTACCATATATGGTGACAAAAACTGAGTCAGCCCGCGATTGGT
GGAAAAACAAACTGGAGCCGATACTGTGTAAATTGTGATAACGGCTCTTTTATATAGTTTATCCTCACGA
GTCGGTTCTCATTTACTAAGGTGTGCTCGAACAGTGCGCATTCGCATCTACGTACTTGTCACTTATTTAA
TAATACTATGTAAGTTTTAATTTTAAAATTGCGAAAGAAAAAAAAACATATTTATTTATTTGTAAAATTT
GAATTTCGAAGGTTCTCCGTCCCTTTACCTTTAAGTATTACATATGTTTGAGTGTTTTTTTTTTTTAATA
ATACGCTAATGATAACGTGTTACGTTACATAATTGTTGCATAACTAGTGAAGTGAAATTTTTTATAAAAA
AAAACATTTTTCGGAATTTAGTGTACTGCAGATGTTAATAAACACTACTAAATAAGAAATAAGTTTATTG
GACGCACATTTCAAAGTGTCCACTCGCATCGATCAATTCGGAAACAGAAATTGGGAACAGTGAATTATGA
ATCTTATACAGTTTTCTTTAACGTCACTAAATAGATGGACGCAAATAAATTTGTCGTTTACTTAGTATAA
TGTATGGAATGAGAATGTAGTTTGAATTGTTTTTTTTCTTTTCTTGCAGACTAATTCAAGAGGTGCGACG
AAGAAGTTGCCGCGTTGGTAGTAGACGGTATCGATAAGCTTGATATCGAATTCCTGCAGCCCAGCATATG
CGCGGTTCGCATCACCATCACCATCACAGCGCTGGATCCTCGATCACAAGTTTGTACAAAAAAGCTGAAC
GAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATA
CTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTT
CCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAAT
GGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCA
TTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCG
TAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCC
GGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTT
TTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTAC
ACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAA
TATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGAC
AACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGG
CGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTA
CTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCG
TATTTGCGCGCACCGGTGCTAGCGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTAT
TACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGG
TAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGG
GATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAAAT
GCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATT
ATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCC
GTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGT
GCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATT
AACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCGTTATACACAGCCAGTCTGCAGGTCGACCATAGT
GACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGA
TATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATAATTAATTAAGATCGAAT
CTAGAGCGGCCGCCACCGCCTCGAGAAGTCTTACGAACTTCCCGACGGTCAGGTCATCACCATCGGAAAC
GAAAGATTCCGTTGCCCAGAGGCCCTCTTCCAACCCTCGTTCTTGGGTATGGAAGCCAACGGAATCCACG
AAACCACATACAACTCCATCATGAAGTGCGACGTGGACATCCGTAAGGACTTGTACGCCAACACCGTATT
GTCCGGTGGTACCACCATGTACCCTGGAATCGCCGACCGTATGCAAAAGGAAATCACACGTCTCGCCCCA
TCGACAATGAAGATTAAGATCATCGCTCCCCCAGAGAGGAAGTACTCCGTATGGATCGGTGGATCGATCC
TCGCCTCCCTCTCTACCTTCCAACAGATGTGGATCTCGAAACAGGAGTACGACGAGTCTGGTCCCTCCAT
TGTACACAGGAAGTGCTTCTAAGCGTTGAGACTTTAAGTTATGATGCCCTACAGCAGAACCTCAAGAGGG
TGGCTCAAATTACGCTTGTGATCTTGTAAATAAATTCAGTATTTAATGTAGGTTGTAAGGTATTGTAATA
TGCATATTACGTAAAACGAACGGAATGTTGTTGTTGCCGTTTTTTTTTTGACAAAGATTTTTATTTATTA
AAGTTACTAACCCCAAAACTTTTTAATAAAATAAATTTATATACCGGTATAATAACTGACGTTTTTCACT
TGCTGTCCCCGCTCCCGACTAACAGTACGTCGTGTGCACCGAAATTACCGATTTCGTACACCGTTTGAGA
CAGTTACGCTAGGAGCACAAATCTCCCAGCTGCATACCGTTGTTTACTGCAGCTGCAGTCTTTAATTGGA
ATGCGAGTCGTTGACCGCTTAATACGAAACATTCTAAAATTCGCAAAATGCAAAGGAAACTGGTTCTGTA
CTTTCTACCTTTCAAAAGATTCACCAAATTAATTTTATGCGGACTCACTAATTCCGTAGAAATCTGTGTA
GAGGTACCCAGGTTACGCTTAGGCATAAGATGACTGTTCGCGTTTTTACAATACATACGAGCAGGTTACA
CACAAGATGAACATCCTTTGATGCGTCTGTGTCTTGACCCGTCTGAGATTTGAGTGACTTGTCAACGTCA
TTGCGTAGTGTCACCGGTCGTCGAGATCCCCGCCGCGGTGGAGCTACGAGCTCCAGCTTTTGTTCCCTTT
AGTGAGGGTTAATTCCGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCT
CACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAA
CTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAAT
GAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTC
GCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACA
GAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCA
GAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCT
CCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACC
CCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGAC
TTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCC
AGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT
TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTT
CACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCT
GACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTG
CCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGAT
ACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGC
AGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTA
GTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTT
TGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAA
AAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGG
TTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTA
CTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGAT
AATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCT
CAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATC
TTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGG
GCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATT
GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCC
CCGAAAAGTGCCACCTGGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAA
TCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGAT
AGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGG
CGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGA
GGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGC
GAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTC
ACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCAT
TCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGG
GGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACG
GCCAGTGAATTGTAATACGACTCACTATAGGGCGAATTGGGTACCGGGCCCCCCCTC

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