pGL4.78[hRlucCP/Hygro]

价格:1500元

联系方式:I47-825O-882O

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pGL4.78载体基本信息

出品公司: Promega
载体名称: pGL4.78 ; pGL4.78[hRlucCP/Hygro]
质粒类型: 信号通路报告载体;哺乳动物载体;海肾荧光素酶报告载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子:
载体大小: 5303 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: hRluc
载体抗性: 氨苄青霉素
筛选标记: 潮霉素(Hygromycin)
克隆菌株: TOP10等常规菌株
宿主细胞(系): HEK293等
备注: pGL4.78[hRlucCP/Hygro]载体是信号通路报告载体;无启动子;含海肾荧光素酶报告基因hRlucCP。
产品目录号: E6961
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pGL4.78载体质粒图谱和多克隆位点信息

pGL4.78载体图谱



pGL4.78 载体特征

pGL4.78载体简介

The pGL4.78[hRlucCP/Hygro] Vector encodes the luciferase reporter gene hRlucCP (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for hygromycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The pGL4 Vectors are engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.78[hRlucCP/Hygro] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice. The hRlucCP reporter gene contains two protein destabilization sequences, hCL1 and hPEST. The protein encoded by hRlucCP responds more quickly and with greater magnitude to changes in transcriptional activity than the hRluc gene, its more stable counterpart.

pGL4.78载体序列

LOCUS       AY864933                5303 bp    DNA     circular SYN 31-JAN-2005
DEFINITION  Luciferase reporter vector pGL4.78[hRlucCP/Hygro], complete
            sequence.
ACCESSION   AY864933
VERSION     AY864933.1  GI:58201885
KEYWORDS    .
SOURCE      Luciferase reporter vector pGL4.78[hRlucCP/Hygro]
  ORGANISM  Luciferase reporter vector pGL4.78[hRlucCP/Hygro]
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Paguio,A., Almond,B. and Browning,J.
  TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Paguio,A., Almond,B. and Browning,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-DEC-2004) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Luciferase reporter vector
                     pGL4.78[hRlucCP/Hygro]"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:309059"
     misc_feature    1..70
                     /note="multiple cloning site"
     CDS             100..1212
                     /note="Renilla luciferase"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase hRlucCP"
                     /protein_id="AAW66997.1"
                     /db_xref="GI:58201886"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                     NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                     AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                     EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                     WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                     EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSACKNWFSSLSHFVIHLNSHGF
                     PPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    1249..1470
                     /note="SV40 late poly A"
     misc_feature    1518..1936
                     /note="SV40 enhancer/promoter"
     CDS             1961..2998
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic hygromycin resistance protein"
                     /protein_id="AAW66998.1"
                     /db_xref="GI:58201887"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKEVGRV"
     regulatory      3022..3070
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic polyadenylation signal sequence"
     primer_bind     3137..3156
                     /note="RVprimer4 binding site"
     rep_origin      3394
                     /note="ColEI-derived plasmid replication origin"
     CDS             complement(4185..5045)
                     /note="Ampr"
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic beta-lactamase"
                     /protein_id="AAW66999.1"
                     /db_xref="GI:58201888"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    5150..5303
                     /note="synthetic poly(A) signal/transcriptional pause
                     site"
     primer_bind     5252..5271
                     /note="RVprimer3 binding site"
ORIGIN      
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
      121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
      181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
      241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
      301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
      361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
      421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
      481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
      541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
      601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
      661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
      721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
      781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
      841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
      901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
      961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
     1021 aagaacgagc agaattctgc ttgcaagaac tggttcagta gcttaagcca ctttgtgatc
     1081 caccttaaca gccacggctt ccctcccgag gtggaggagc aggccgccgg caccctgccc
     1141 atgagctgcg cccaggagag cggcatggat agacaccctg ctgcttgcgc cagcgccagg
     1201 atcaacgtct aactgcagtc tagagtcggg gcggccggcc gcttcgagca gacatgataa
     1261 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     1321 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     1381 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     1441 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgttt gcgtattggg
     1501 cgctcttccg ctgatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg
     1561 gttagctacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     1621 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     1681 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     1741 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     1801 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     1861 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     1921 cttttgcaaa aagctcgatt cttctgacac tagcgccacc atgaagaagc ccgaactcac
     1981 cgctaccagc gttgaaaaat ttctcatcga gaagttcgac agtgtgagcg acctgatgca
     2041 gttgtcggag ggcgaagaga gccgagcctt cagcttcgat gtcggcggac gcggctatgt
     2101 actgcgggtg aatagctgcg ctgatggctt ctacaaagac cgctacgtgt accgccactt
     2161 cgccagcgct gcactaccca tccccgaagt gttggacatc ggcgagttca gcgagagcct
     2221 gacatactgc atcagtagac gcgcccaagg cgttactctc caagacctcc ccgaaacaga
     2281 gctgcctgct gtgttacagc ctgtcgccga agctatggat gctattgccg ccgccgacct
     2341 cagtcaaacc agcggcttcg gcccattcgg gccccaaggc atcggccagt acacaacctg
     2401 gcgggatttc atttgcgcca ttgctgatcc ccatgtctac cactggcaga ccgtgatgga
     2461 cgacaccgtg tccgccagcg tagctcaagc cctggacgaa ctgatgctgt gggccgaaga
     2521 ctgtcccgag gtgcgccacc tcgtccatgc cgacttcggc agcaacaacg tcctgaccga
     2581 caacggccgc atcaccgccg taatcgactg gtccgaagct atgttcgggg acagtcagta
     2641 cgaggtggcc aacatcttct tctggcggcc ctggctggct tgcatggagc agcagactcg
     2701 ctacttcgag cgccggcatc ccgagctggc cggcagccct cgtctgcgag cctacatgct
     2761 gcgcatcggc ctggatcagc tctaccagag cctcgtggac ggcaacttcg acgatgctgc
     2821 ctgggctcaa ggccgctgcg atgccatcgt ccgcagcggg gccggcaccg tcggtcgcac
     2881 acaaatcgct cgccggagcg cagccgtatg gaccgacggc tgcgtcgagg tgctggccga
     2941 cagcggcaac cgccggccca gtacacgacc gcgcgctaag gaggtaggtc gagtttaaac
     3001 tctagaaccg gtcatggccg caataaaata tctttatttt cattacatct gtgtgttggt
     3061 tttttgtgtg ttcgaactag atgctgtcga ccgatgccct tgagagcctt caacccagtc
     3121 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     3181 atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc
     3241 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3301 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3361 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3421 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3481 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3541 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3601 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3661 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3721 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3781 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3841 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3901 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3961 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4021 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4081 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4141 aacttggtct gacagcggcc gcaaatgcta aaccactgca gtggttacca gtgcttgatc
     4201 agtgaggcac cgatctcagc gatctgccta tttcgttcgt ccatagtggc ctgactcccc
     4261 gtcgtgtaga tcactacgat tcgtgagggc ttaccatcag gccccagcgc agcaatgatg
     4321 ccgcgagagc cgcgttcacc ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg
     4381 gccgagcgaa gaagtggtcc tgctactttg tccgcctcca tccagtctat gagctgctgt
     4441 cgtgatgcta gagtaagaag ttcgccagtg agtagtttcc gaagagttgt ggccattgct
     4501 actggcatcg tggtatcacg ctcgtcgttc ggtatggctt cgttcaactc tggttcccag
     4561 cggtcaagcc gggtcacatg atcacccata ttatgaagaa atgcagtcag ctccttaggg
     4621 cctccgatcg ttgtcagaag taagttggcc gcggtgttgt cgctcatggt aatggcagca
     4681 ctacacaatt ctcttaccgt catgccatcc gtaagatgct tttccgtgac cggcgagtac
     4741 tcaaccaagt cgttttgtga gtagtgtata cggcgaccaa gctgctcttg cccggcgtct
     4801 atacgggaca acaccgcgcc acatagcagt actttgaaag tgctcatcat cgggaatcgt
     4861 tcttcggggc ggaaagactc aaggatcttg ccgctattga gatccagttc gatatagccc
     4921 actcttgcac ccagttgatc ttcagcatct tttactttca ccagcgtttc ggggtgtgca
     4981 aaaacaggca agcaaaatgc cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg
     5041 ctcatactcg tcctttttca atattattga agcatttatc agggttacta gtacgtctct
     5101 caaggataag taagtaatat taaggtacgg gaggtattgg acaggccgca ataaaatatc
     5161 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc
     5221 tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag
     5281 tgcaggtgcc agaacatttc tct
//

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