pRetroX-PTuner2

价格:3500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pRetroX-PTuner2
质粒类型: 逆病毒载体;双顺反子载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV IE
载体大小: 7345 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: DD tag (N-端)
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: DH5α , Stellar
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 逆病毒载体pRetroX-PTuner2载体表达N端DD标签融合蛋白;
DD标签是一个大小为12KD的FKBP (L106P)去稳定结构域,带有DD标签的目的蛋白将很快被降解;
向培养基中加入Shield1配体后,情况将发生逆转,细胞中目的蛋白的量则迅速提高。
产品目录号: 631097
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pRetroX-PTuner2载体图谱



pRetroX-PTuner2 多克隆位点

pRetroX-PTuner2 载体特征

载体简介

载体序列

LOCUS       pRetroX-PTuner2   7345 bp    DNA   circular            11-APR-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631097,631093
FEATURES             Location/Qualifiers
     misc_feature    1..727
                     /label=CMV/MSV\5'\LTR
     misc_feature    757..1566
                     /label=Psi\-\Extended\packaging\signal
     misc_feature    4044..4476
                     /label=3'\MMLV\LTR\(deletion\in\U3)
     CDS             complement(6123..6983)
                     /label=Amp(R)
     promoter        2731..3239
                     /label=PGK\Promoter
     CDS             3260..3859
                     /label=Puro(R)
     polyA_signal    4302..4317
                     /label=PolyA\signal
     rep_origin      4977..5042
                     /label=SV40\origin
     promoter        1582..2170
                     /label=CMV\IE\Promoter
     promoter        4756..5023
                     /label=SV40\Promoter
     rep_origin      5363..5363
                     /label=pUC\origin
     CDS             2178..2654
                     /label=DD-G
BASE COUNT     1661 a      2059 c      1910 g      1715 t 
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca 
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 
     1861 cgccttgcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg 
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt 
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac 
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa 
     2161 ccgtcagatc cgctagcatg atcagtctga ttgcggcgtt agcggtagat ctcgttatcg 
     2221 gcatggaaaa cgccatgccg tggaacctgc ctgccgatct cgcctggttt aaacgcaaca 
     2281 ccttaaataa acccgtgatt atgggccgcc atacctggga atcaatcggt cgtccgttgc 
     2341 caggacgcaa aaatattatc ctcagcagtc aaccgagtac ggacgatcgc gtaacgtggg 
     2401 tgaagtcggt ggatgaagcc atcgcggcgt gtggtgacgt accagaaatc atggtgattg 
     2461 gcggcggtcg cgttattgaa cagttcttgc caaaagcgca aaaactgtat ctgacgcata 
     2521 tcgacgcaga agtggaaggc gacactcatt tcccggatta cgagccggat gactgggaat 
     2581 cggtattcag cgaattccac gatgctgatg cgcagaactc tcacagctat tgctttgaga 
     2641 ttctggagcg gcgactcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggccc 
     2701 gggatccacc ggatctagat aactgatcat aattctaccg ggtaggggag gcgcttttcc 
     2761 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg 
     2821 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg 
     2881 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc 
     2941 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga 
     3001 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc 
     3061 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca 
     3121 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca 
     3181 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga 
     3241 cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 
     3301 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 
     3361 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 
     3421 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 
     3481 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 
     3541 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc 
     3601 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 
     3661 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 
     3721 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 
     3781 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac 
     3841 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc 
     3901 acgaccccat gcatcggcgt ctcgatcgag atatcagtgg tccaggctct agttttgact 
     3961 caacaatatc accagctgaa gcctatagag tacgagccat agataaaata aaagatttta 
     4021 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctacta 
     4081 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc 
     4141 agatcaaggt caggaacaga tggaacaggg tcgatcgacc ctagagaacc atcagatgtt 
     4201 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt 
     4261 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc 
     4321 cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat 
     4381 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct 
     4441 gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag 
     4501 acccctgccc agggaccacc gacccaccac cgggaggtaa gctggctgcc tcgcgcgttt 
     4561 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 
     4621 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 
     4681 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtagatcc ggctgtggaa 
     4741 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 
     4801 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 
     4861 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 
     4921 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 
     4981 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 
     5041 aggctttttt ggaggcctag gcttttgcaa aaagcttact ggcttaacta tgcggcatca 
     5101 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg 
     5161 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
     5221 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
     5281 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
     5341 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
     5401 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
     5461 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
     5521 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
     5581 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
     5641 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     5701 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     5761 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 
     5821 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     5881 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     5941 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     6001 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     6061 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     6121 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     6181 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     6241 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     6301 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     6361 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     6421 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     6481 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     6541 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     6601 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     6661 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     6721 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg 
     6781 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     6841 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     6901 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     6961 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     7021 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     7081 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 
     7141 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attagcttgg 
     7201 ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca 
     7261 ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca 
     7321 ttagttcata gcccatatat ggagt 
//

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