| 出品公司: |
Invitrogen
|
|---|---|
| 载体名称: | pUB6/V5 His A |
| 质粒类型: | 哺乳细胞表达系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | UbC |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5463bp |
| 5' 测序引物及序列: | T7 Fwd：5'd[TAATACGACTCACTATAGGG]3' |
| 3' 测序引物及序列: | -- |
| 载体标签: | 6X His, V5 |
| 载体抗性: | Ampicillin |
| 筛选标记: | Blasticidin |
| 备注: | 可以严格控制表达水平 |
| 产品目录号: | V25020 |
| 稳定性: | 瞬表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pUB6/V5 His A载体 pUB6-V5HisA质粒基本信息
pUB6/V5 His A质粒图谱 pUB6-V5HisA载体图谱和pUB6/V5 His A载体序列 pUB6-V5HisA质粒序列多克隆位点信息
pUB6/V5 His A质粒 pUB6-V5HisA载体简介
pUB6/V5 His A质粒序列 pUB6-V5HisA载体序列
LOCUS pUB6/V5/His A 5463 bp DNA SYN
DEFINITION pUB6/V5/His A
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5463
/organism="pUB6/V5/His A"
/mol_type="other DNA"
promoter 13..1227
/label="hUbC_promoter"
misc_feature complement(1418..1435)
/label="BGH_rev_primer"
terminator 1421..1648
/label="bGH_PA_terminator"
rep_origin 1711..2017
/label="f1_origin"
misc_feature complement(2131..2151)
/label="pBABE_3_primer"
misc_feature complement(2137..2353)
/label="SV40_enhancer"
promoter 2149..2418
/label="SV40_promoter"
rep_origin 2317..2394
/label="SV40_origin"
misc_feature 2379..2398
/label="SV40pro_F_primer"
promoter 2512..2579
/label="EM7_promoter"
gene 2580..2978
/label="blast"
/gene="blast"
terminator 3139..3258
/label="SV40_PA_terminator"
misc_feature 3227..3246
/label="EBV_rev_primer"
promoter complement(3302..3320)
/label="M13_reverse_primer"
misc_feature complement(3319..3341)
/label="M13_pUC_rev_primer"
promoter complement(3355..3384)
/label="lac_promoter"
rep_origin complement(3693..4312)
/label="pBR322_origin"
gene complement(4467..5327)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(4467..5327)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5369..5397)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 GACGGATCGG GAGATCTGGC CTCCGCGCCG GGTTTTGGCG CCTCCCGCGG GCGCCCCCCT
61 CCTCACGGCG AGCGCTGCCA CGTCAGACGA AGGGCGCAGG AGCGTCCTGA TCCTTCCGCC
121 CGGACGCTCA GGACAGCGGC CCGCTGCTCA TAAGACTCGG CCTTAGAACC CCAGTATCAG
181 CAGAAGGACA TTTTAGGACG GGACTTGGGT GACTCTAGGG CACTGGTTTT CTTTCCAGAG
241 AGCGGAACAG GCGAGGAAAA GTAGTCCCTT CTCGGCGATT CTGCGGAGGG ATCTCCGTGG
301 GGCGGTGAAC GCCGATGATT ATATAAGGAC GCGCCGGGTG TGGCACAGCT AGTTCCGTCG
361 CAGCCGGGAT TTGGGTCGCG GTTCTTGTTT GTGGATCGCT GTGATCGTCA CTTGGTGAGT
421 AGCGGGCTGC TGGGCTGGCC GGGGCTTTCG TGGCCGCCGG GCCGCTCGGT GGGACGGAAG
481 CGTGTGGAGA GACCGCCAAG GGCTGTAGTC TGGGTCCGCG AGCAAGGTTG CCCTGAACTG
541 GGGGTTGGGG GGAGCGCAGC AAAATGGCGG CTGTTCCCGA GTCTTGAATG GAAGACGCTT
601 GTGAGGCGGG CTGTGAGGTC GTTGAAACAA GGTGGGGGGC ATGGTGGGCG GCAAGAACCC
661 AAGGTCTTGA GGCCTTCGCT AATGCGGGAA AGCTCTTATT CGGGTGAGAT GGGCTGGGGC
721 ACCATCTGGG GACCCTGACG TGAAGTTTGT CACTGACTGG AGAACTCGGT TTGTCGTCTG
781 TTGCGGGGGC GGCAGTTATG CGGTGCCGTT GGGCAGTGCA CCCGTACCTT TGGGAGCGCG
841 CGCCCTCGTC GTGTCGTGAC GTCACCCGTT CTGTTGGCTT ATAATGCAGG GTGGGGCCAC
901 CTGCCGGTAG GTGTGCGGTA GGCTTTTCTC CGTCGCAGGA CGCAGGGTTC GGGCCTAGGG
961 TAGGCTCTCC TGAATCGACA GGCGCCGGAC CTCTGGTGAG GGGAGGGATA AGTGAGGCGT
1021 CAGTTTCTTT GGTCGGTTTT ATGTACCTAT CTTCTTAAGT AGCTGAAGCT CCGGTTTTGA
1081 ACTATGCGCT CGGGGTTGGC GAGTGTGTTT TGTGAAGTTT TTTAGGCACC TTTTGAAATG
1141 TAATCATTTG GGTCAATATG TAATTTTCAG TGTTAGACTA GTAAATTGTC CGCTAAATTC
1201 TGGCCGTTTT TGGCTTTTTT GTTAGACGAA GCTTGGTACC GAGCTCGGAT CCACTAGTCC
1261 AGTGTGGTGG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC
1321 TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTCAT
1381 CATCACCATC ACCATTGAGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
1441 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC
1501 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT
1561 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG
1621 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG CTGGGGCTCT
1681 AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG
1741 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT
1801 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG CATCCCTTTA
1861 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT
1921 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG
1981 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT
2041 TCTTTTGATT TATAAGGGAT TTTGGGGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT
2101 TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT
2161 CCCCAGGCTC CCCAGGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC
2221 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT
2281 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT
2341 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC
2401 GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT
2461 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAGCA CGTGTTGACA
2521 ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA
2581 TGGCCAAGCC TTTGTCTCAA GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA
2641 ACAGCATCCC CATCTCTGAA GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC
2701 GCATCTTCAC TGGTGTCAAT GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG
2761 TGCTGGGCAC TGCTGCTGCT GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA
2821 ATGAGAACAG GGGCATCTTG AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC
2881 ATCCTGGGAT CAAAGCCATA GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC
2941 GTGAATTGCT GCCCTCTGGT TATGTGTGGG AGGGCTAAGC ACTTCGTGGC CGAGGAGCAG
3001 GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC
3061 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG
3121 TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
3181 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA
3241 CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA
3301 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA
3361 CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA
3421 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA
3481 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG
3541 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG
3601 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
3661 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC
3721 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
3781 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG
3841 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT
3901 CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
3961 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG
4021 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
4081 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC
4141 ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA
4201 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
4261 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG
4321 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
4381 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
4441 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
4501 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
4561 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA
4621 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
4681 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
4741 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
4801 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
4861 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
4921 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
4981 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
5041 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
5101 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
5161 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
5221 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
5281 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
5341 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
5401 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC
5461 GTC
//
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pUB6/V5 His A质粒 pUB6-V5HisA载体应用举例
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