| 质粒名称: | pHR-DDX4N-mCh-Cry2WT |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 101225 |
| 存储实验室: | Clifford Brangwynne |
|
载体骨架基本信息 |
|
| 载体名称: | pHR |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | DDX4(1-236) |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHR-DDX4N-mCh-Cry2WT was a gift from Clifford Brangwynne (Addgene plasmid # 101225 ; http://n2t.net/addgene:101225 ; RRID:Addgene_101225) |
| 参考文献部分: | Spatiotemporal Control of Intracellular Phase Transitions Using Light-Activated optoDroplets. Shin Y, Berry J, Pannucci N, Haataja MP, Toettcher JE, Brangwynne CP. Cell. 2017 Jan 12;168(1-2):159-171.e14. doi: 10.1016/j.cell.2016.11.054. Epub 2016 Dec 29. 10.1016/j.cell.2016.11.054 PubMed 28041848 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHR-DDX4N-mCh-Cry2WT 12020 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION DDX4(1-236) fused to mCh-Cry2WT.
ACCESSION .
VERSION .
KEYWORDS pHR-DDX4N-mCh-Cry2WT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12020)
AUTHORS Shin Y, Berry J, Pannucci N, Haataja MP, Toettcher JE, Brangwynne CP
TITLE Spatiotemporal Control of Intracellular Phase Transitions Using
Light-Activated optoDroplets.
JOURNAL Cell. 2017 Jan 12;168(1-2):159-171.e14. doi:
10.1016/j.cell.2016.11.054. Epub 2016 Dec 29.
PUBMED 28041848
REFERENCE 2 (bases 1 to 12020)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12020
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 43..160
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 313..720
/label=SFFV promoter
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
misc_feature 3877..4465
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3930..3950)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4348..4359)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4468..4484)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4469..4485)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 4575..4808
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(4833..4851)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(4834..4851)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(5141..5160)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(5374..5393)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5493..5515
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(5553..5571)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 5639..5743
/gene="bla"
/label=AmpR promoter
CDS 5744..6604
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5962..5981)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6775..7363
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7264..7283
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7517..7534
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(7604..7624)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7609..7938
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7789..7924
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7851..7870
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 9072..9137
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 9267..9287
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 9712..9846
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9749..9768)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9803..9822
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 10014..10647
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 10694..10819
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 11316..11549
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
ORIGIN
1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
721 ggggatctgg agctctcgag aattctcacg cgtcaagtgg agcaaggcag gtggacagtg
781 gatccggagc taccatggga gatgaagatt gggaagcaga aatcaaccct catatgtctt
841 cctatgttcc catatttgag aaggataggt attctggaga aaatggagac aattttaaca
901 ggactccagc ttcatcatca gaaatggatg atggaccttc tcgaagagat catttcatga
961 aaagtggatt tgcctctggg cggaattttg gaaacagaga tgctggtgag tgtaataagc
1021 gagataatac atccacaatg ggtggttttg gagttggaaa gagttttgga aacagaggtt
1081 tttcaaacag caggtttgaa gatggtgata gctctggttt ctggagagag tctagtaatg
1141 actgcgaaga taatccaaca cggaacagag ggttttccaa gagaggcggc tatcgagatg
1201 gaaataattc agaagcttca gggccataca gaagaggtgg aagaggtagt ttccgaggtt
1261 gccgtggagg atttggtcta ggaagtccaa ataatgactt agacccagac gaatgtatgc
1321 agcgcactgg tggccttttt ggttctagaa gaccagtatt aagtggcaca ggtaatggtg
1381 atacttctca aagcagaagt ggcagtggaa gtgaacgagg tggttacaaa ggtttaaatg
1441 aagaagtaat aacaggctct ggaaagaatt cttggaagtc agaagcagaa ggaggagaaa
1501 gtggcggcgg catggtgtct aaaggcgagg aggataacat ggctataatc aaggaattta
1561 tgaggttcaa ggtgcatatg gagggatctg tgaacggtca cgagttcgaa atcgagggcg
1621 aaggcgaggg gcgaccctat gaagggacac agacggccaa actgaaggtg accaagggtg
1681 gccccctgcc tttcgcctgg gacatcctgt caccccagtt tatgtacggc tctaaggctt
1741 acgttaagca ccctgccgat attccggact acttgaaact gagcttccct gaagggttca
1801 agtgggaacg ggtaatgaat tttgaggatg gcggtgtagt cacagttacc caggacagct
1861 ctcttcagga cggagaattt atctataagg ttaaacttcg gggcactaac ttcccatcag
1921 acggccccgt gatgcagaag aaaactatgg gctgggaagc cagctcagaa cgcatgtatc
1981 ccgaggacgg agcactgaag ggagaaatca agcagcgact caagctgaag gatgggggac
2041 attatgatgc tgaggtcaaa accacctata aggccaagaa acccgttcag cttcctgggg
2101 catacaacgt gaatatcaaa ctggatatta catcccataa cgaagactat accatcgtgg
2161 aacagtacga gcgggccgag ggcagacata gcacaggggg catggatgaa ttgtacaagg
2221 cggccacgcg tatgaagatg gacaaaaaga ctatagtttg gtttagaaga gacctaagga
2281 ttgaggataa tcctgcatta gcagcagctg ctcacgaagg atctgttttt cctgtcttca
2341 tttggtgtcc tgaagaagaa ggacagtttt atcctggaag agcttcaaga tggtggatga
2401 aacaatcact tgctcactta tctcaatcct tgaaggctct tggatctgac ctcactttaa
2461 tcaaaaccca caacacgatt tcagcgatct tggattgtat ccgcgttacc ggtgctacaa
2521 aagtcgtctt taaccacctc tatgatcctg tttcgttagt tcgggaccat accgtaaagg
2581 agaagctggt ggaacgtggg atctctgtgc aaagctacaa tggagatcta ttgtatgaac
2641 cgtgggagat atactgcgaa aagggcaaac cttttacgag tttcaattct tactggaaga
2701 aatgcttaga tatgtcgatt gaatccgtta tgcttcctcc tccttggcgg ttgatgccaa
2761 taactgcagc ggctgaagcg atttgggcgt gttcgattga agaactaggg ctggagaatg
2821 aggccgagaa accgagcaat gcgttgttaa ctagagcttg gtctccagga tggagcaatg
2881 ctgataagtt actaaatgag ttcatcgaga agcagttgat agattatgca aagaacagca
2941 agaaagttgt tgggaattct acttcactac tttctccgta tctccatttc ggggaaataa
3001 gcgtcagaca cgttttccag tgtgcccgga tgaaacaaat tatatgggca agagataaga
3061 acagtgaagg agaagaaagt gcagatcttt ttcttagggg aatcggttta agagagtatt
3121 ctcggtatat atgtttcaac ttcccgttta ctcacgagca atcgttgttg agtcatcttc
3181 ggtttttccc ttgggatgct gatgttgata agttcaaggc ctggagacaa ggcaggaccg
3241 gttatccgtt ggtggatgcc ggaatgagag agctttgggc taccggatgg atgcataaca
3301 gaataagagt gattgtttca agctttgctg tgaagtttct tctccttcca tggaaatggg
3361 gaatgaagta tttctgggat acacttttgg atgctgattt ggaatgtgac atccttggct
3421 ggcagtatat ctctgggagt atccccgatg gccacgagct tgatcgcttg gacaatcccg
3481 cgttacaagg cgccaaatat gacccagaag gtgagtacat aaggcaatgg cttcccgagc
3541 ttgcgagatt gccaactgaa tggatccatc atccatggga cgctccttta accgtactca
3601 aagcttctgg tgtggaactc ggaacaaact atgcgaaacc cattgtagac atcgacacag
3661 ctcgtgagct actagctaaa gctatttcaa gaacccgtga agcacagatc atgatcggag
3721 cagcagcccg ggatccaccg gtcgccaccg gttcagggag cggatccggc tcagggagtg
3781 cggccgcaac tcccacctgc aacatgcgtg actgactgag gccgcgactc tagagtcgac
3841 ctgcaggcat gcaagcttga tatcaagctt atcgataatc aacctctgga ttacaaaatt
3901 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
3961 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
4021 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
4081 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
4141 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
4201 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
4261 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
4321 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
4381 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
4441 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagggaa ttaattcgag
4501 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
4561 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
4621 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
4681 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
4741 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
4801 ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt
4861 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct
4921 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc
4981 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa
5041 atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc
5101 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
5161 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
5221 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
5281 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
5341 cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
5401 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
5461 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
5521 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
5581 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
5641 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
5701 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
5761 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
5821 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
5881 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
5941 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
6001 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
6061 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
6121 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
6181 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
6241 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
6301 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
6361 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
6421 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
6481 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
6541 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
6601 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
6661 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
6721 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
6781 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
6841 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
6901 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
6961 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
7021 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
7081 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
7141 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
7201 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
7261 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
7321 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
7381 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
7441 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
7501 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
7561 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta
7621 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
7681 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
7741 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
7801 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
7861 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
7921 ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat
7981 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
8041 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
8101 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
8161 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
8221 atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt
8281 gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag
8341 cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat
8401 gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac
8461 tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat
8521 atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca
8581 atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc
8641 aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa
8701 cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg
8761 ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc
8821 tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga
8881 aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt
8941 atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct
9001 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt
9061 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa
9121 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg
9181 cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta
9241 ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg
9301 actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg
9361 cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg
9421 aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt
9481 ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta
9541 cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga
9601 ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
9661 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
9721 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
9781 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
9841 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
9901 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
9961 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg
10021 gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg
10081 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt
10141 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
10201 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
10261 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
10321 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga
10381 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat
10441 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
10501 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
10561 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
10621 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
10681 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
10741 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
10801 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
10861 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
10921 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
10981 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
11041 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
11101 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
11161 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
11221 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
11281 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
11341 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
11401 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
11461 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
11521 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
11581 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
11641 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
11701 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
11761 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
11821 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
11881 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
11941 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
12001 agatccattc gattagtgaa
//
