pYC66

价格:1200元

联系方式:I47-825O-882O

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载体基本信息

出品人: Irene Castano Navarro(2015)
载体名称: pYC66
质粒类型: 酿酒酵母载体;复制型载体;抗性筛选载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子:
载体大小: 5085 bp
5' 测序引物及序列: M13/pUC fwd primer 5’ CCAGTCACGACGTTGTAAAACG 3’
3' 测序引物及序列: M13 rev primer 5’ GGAAACAGCTATGACCATG 3’
载体标签: 3XMyc tag (C-ter)
载体抗性: 氨苄青霉素
筛选标记: Nourseothricin
克隆菌株: DH10B感受态细胞 ,30°C
宿主细胞(系): 出芽酵母(酿酒酵母)、白色念珠菌
备注: --
产品目录号: #63929
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pYC66载体图谱



pYC66多克隆位点

载体简介

载体序列

LOCUS       pYC66	5085 bp 	DNA	SYN
DEFINITION  pYC66
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="pYC66"
                     /mol_type="other DNA"
     CDS             761..1333
                     /label="ORF frame 2"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     terminator      1347..1586
                     /label="CYC1_terminator"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     misc_feature    complement(1356..1374)
                     /label="CYC1_primer"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     misc_feature    complement(1356..1374)
                     /label="pYESTrp_rev_primer"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     misc_feature    1608..1624
                     /label="pBluescriptKS_primer"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     promoter        complement(2287..2306)
                     /label="T3_promoter"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     promoter        complement(2323..2341)
                     /label="M13_reverse_primer"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     misc_feature    complement(2340..2362)
                     /label="M13_pUC_rev_primer"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     promoter        complement(2376..2405)
                     /label="lac_promoter"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     gene            complement(3488..4348)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     CDS             complement(3488..4348)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(4390..4418)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcaCctcccg gagacggtca
   61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
  121 ttggcgggtg tcggggctgg cttaactatg cgGCATCAGA GCAGATTGTA CTGAGAGTGC
  181 ACCATAccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
  241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
  301 agcacagact tagattggta tatatacgCA TACcataaag cacAGCTCca tagcttcaaa
  361 atgtttctac tcctttttta ctcttccaga ttttctcgga ctccgcgcat cgccgtacca
  421 cttcaaaaca cccaagcaca gcatactaaa tttcccctct ttcttcctct agggtgtcgt
  481 taattacccg tactaaaggt ttggaaaaga aaaaagagac cgcctcgttt ctttttcttc
  541 gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt cttgaaaatt ttttttttga
  601 tttttttctc tttcgatgac ctcccattga tatttaagtt aataaacggt cttcaatttc
  661 tcaagtttca gtttcatttt tcttgttcta ttacaacttt ttttacttct tgctcattag
  721 aaagaaagca tagcaatcta atctaagttt tggatctaaa ATGGGCACCA CTCTTGACGA
  781 CACGGCTTAC CGATACCGCA CCAGTGTCCC GGGGGACGCC GAGGCCATCG AGGCACTGGA
  841 TGGGTCCTTC ACCACCGACA CCGTCTTCCG CGTCACCGCC ACCGGGGACG GCTTCACCCT
  901 GCGGGAGGTG CCGGTGGACC CGCCCCTGAC CAAGGTGTTC CCCGACGACG AATCGGACGA
  961 CGAATCGGAC GACGGGGAGG ACGGCGACCC TGACTCCCGG ACGTTCGTCG CGTACGGGGA
 1021 CGACGGCGAC CTGGCGGGCT TCGTGGTCGT CTCGTACTCC GGCTGGAACC GCCGGCTGAC
 1081 CGTCGAGGAC ATCGAGGTCG CCCCGGAGCA CCGGGGGCAC GGGGTCGGGC GCGCGTTGAT
 1141 GGGGCTCGCG ACGGAGTTCG CCCGCGAGCT GGGCGCCGGG CACCTCTGGC TGGAGGTCAC
 1201 CAACGTCAAC GCACCGGCGA TCCACGCGTA CCGGCGGATG GGGTTCACCC TCTGCGGCCT
 1261 GGACACCGCC CTGTACGACG GCACCGCCTC GGACGGCGAG CAGGCGCTCT ACATGAGCAT
 1321 GCCCTGCCCC TAAgtcgagt catgtaatta gttatgtcac gcttacattc acgccctccc
 1381 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt
 1441 tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
 1501 tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt
 1561 ttgggacgct cgaaggcttt aatttgcggc cGGTACcggg ccccccctcg aggtcgacgg
 1621 tatcgataag cttgatatcg aattcctgca gcccggggga tcctagggTT AATTAACGGT
 1681 GAACAAAAGC TAATCTCCGA GGAAGACTTG AACGGTGAAC AAAAATTAAT CTCAGAAGAA
 1741 GACTTGAACG GACTCGACGG TGAACAAAAG TTGATTTCTG AAGAAGATTT GAACGGTGAA
 1801 CAAAAGCTAA TCTCCGAGGA AGACTTGAAC GGTGAACAAA AATTAATCTC AGAAGAAGAC
 1861 TTGAACGGAC TCGACGGTGA ACAAAAGTTG ATTTCTGAAG AAGATTTGAA CGGTGAACAA
 1921 AAGCTAATCT CCGAGGAAGA CTTGAACGGT GAACAAAAAT TAATCTCAGA AGAAGACTTG
 1981 AACGGACTCG ACGGTGAACA AAAGTTGATT TCTGAAGAAG ATTTGAACGG TGAACAAAAG
 2041 CTAATCTCCG AGGAAGACTT GAACGGTGAA CAAAAATTAA TCTCAGAAGA AGACTTGAAC
 2101 GGACTCGACG GTGAACAAAA GTTGATTTCT GAAGAAGATT TGAACGGTGA ACAAAAGCTA
 2161 ATCTCCGAGG AAGACTTGAA CGGTGAACAA AAATTAATCA ATCACtagct agtgaattcg
 2221 cgccagatcc GAAGTTCCTA TACTTTctag agcggccgcc accgCGGTGG AGCTCCAGCT
 2281 TTTGTTCCCT TTAGTGAGGG TTAATTGCGC GCTTGGCGTA ATCATGGTCA TAGCTGTTTC
 2341 Ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat aCgagccgga agcataaagt
 2401 gtaaagcctg gggtgcctaa tgagtgagCt aactcacatt aattgcgttg cgctcactgc
 2461 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
 2521 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
 2581 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
 2641 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
 2701 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
 2761 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
 2821 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
 2881 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
 2941 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
 3001 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
 3061 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
 3121 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
 3181 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
 3241 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
 3301 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
 3361 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
 3421 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
 3481 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
 3541 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
 3601 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
 3661 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
 3721 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
 3781 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
 3841 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
 3901 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
 3961 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
 4021 gcttttctgt gactggtgAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC
 4081 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA
 4141 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT
 4201 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT
 4261 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA
 4321 GGGCGACACG GAAATGTTGA ATACTCATac tcttcctttt tcaatattat tgaagcattt
 4381 atcagggtta ttgtctcatg agcggataca taTTTGAATG TGGGGATCTC TAATGATGCA
 4441 ATTTTTGTTT TGTACACTAA GATTCTATTT CGGAACTCTT ACCCATGTCA TTATTTactt
 4501 tattttcatt attgTAATTT TTTAAGCTTT TATTATGAAT TATTTAAATA CAATTCTTTT
 4561 TGATCATGTG ACAGACATCA CTGAAAGATG TGCTCTGCGT TATTAGTCAG ATTGAAATAT
 4621 ACATTTTCAT GTAGATATGA TGTTCTTACT TTAAATGTTG TTCCATTTGC TAGCTTAAAT
 4681 AGACGATATT ATAAATTTAA GTATTCACTA AAAATCTGAC CCATTAATGT TAGAATTAGT
 4741 ATTATTTTTT GTGATATTAC ATTTTTATAT TTTGGATTTC AACCAATGCT CTGCGATATG
 4801 ATTCAACAGA ATAATATGTA CAAGCTACTT TAATTCATAT ATACTAATAG ATTCACAGAT
 4861 CCAGTACGAC TGCAAGAATT TACGTTTTGT TTGAAATGGT AGTAGCTACA CTAAATTAAC
 4921 AATGATCGAT TAATCCACAT CCACAGTGTG TAATCATCCT AATGAAAACA AGAATAACTA
 4981 CAAAAATGAA TATAAACTTT TTGCATGTTA GTCTAACCTG CTAAGAAACC ATTATTATCA
 5041 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTC
//

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