pcDNA3.1/GS

价格:1500元

联系方式:I47-825O-882O

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pcDNA3.1GS载体基本信息

出品公司: Invitrogen
载体名称: pcDNA3.1/GS , pcDNA3.1GS
质粒类型: 哺乳动物细胞表达载体
启动子: CMV
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4020 bp
5' 测序引物:   pcDNA3.1-F:CTAGAGAACCCACTGCTTAC
3' 测序引物:   pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG
载体标签: C-His,  C-V5-Epitope
载体抗性: Ampicillin 氨苄
筛选标记: Neomycin (G418)
备注:
 
产品目录号: H-K1000
稳定性: 瞬表达 Transient
组成型: 组成型 Constitutive
病毒/非病毒: 非病毒

pcDNA3.1GS载体质粒图谱和多克隆位点信息

pcDNA3.1/GS载体图谱

pcDNA3.1GS载体简介

GeneStorm Expression-Ready Clones are expressed from the vector pcDN3.1/GS. This vector utilizes the strong immediate-early cytomegalovirus (CMV) promoter for high-level, constitutive expression in mammalian cell lines. Each GeneStorm Clone is fused to a C-terminal peptide encoding the V5 epitope for detection with the Anti-V5-HRP Antibody and a 6His tag for purification on metal-chelating resin (i.e. ProBond). The vector also encodes the Zeocin resistance gene for selection in E. coli and for the  creation of stable mammalian cell lines.

pcDNA3.1GS载体序列

LOCUS       pcDNA3.1/GS             4020 bp    DNA     circular   SYN  2-SEP-2003
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..4020
                     /invitrogen="138"
                     /vntifkey="98"
     CDS             82..99
                     /invitrogen="1010000"
                     /vntifkey="4"
                     /label=6xHis
     polyA_site      128..352
                     /invitrogen="1870000"
                     /vntifkey="26"
                     /label=BGH\pA
     primer          complement(122..139)
                     /invitrogen="1910000"
                     /vntifkey="27"
                     /label=BGH\reverse\primer
     primer          3876..3896
                     /invitrogen="1930000"
                     /vntifkey="27"
                     /label=CMV\forward\primer
     promoter        3339..3926
                     /invitrogen="2180000"
                     /vntifkey="29"
                     /label=CMV\promoter
     promoter        1216..1283
                     /invitrogen="2340000"
                     /vntifkey="30"
                     /label=EM7\promoter
     rep_origin      398..826
                     /invitrogen="2500000"
                     /vntifkey="33"
                     /label=f1\origin
     rep_origin      complement(2301..2974)
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     promoter        831..1201
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     polyA_signal    1788..1918
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
     primer          3970..3989
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     promoter        3970..3986
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     protein_bind    4016..4020
                     /invitrogen="2460000"
                     /vntifkey="31"
                     /label=TOPO\binding\site
     protein_bind    complement(1..5)
                     /invitrogen="2460000"
                     /vntifkey="31"
                     /label=TOPO\binding\site
     primer          complement(40..60)
                     /invitrogen="2140000"
                     /vntifkey="27"
                     /label=V5\reverse\primer
     CDS             31..72
                     /invitrogen="1350000"
                     /vntifkey="4"
                     /label=V5\epitope
     CDS             1284..1658
                     /invitrogen="1370000"
                     /vntifkey="4"
                     /label=Zeo(R)
BASE COUNT      905 a      1068 c      1049 g       998 t
ORIGIN
        1 aagggcgagc ttcgaggtca cccattcgaa ggtaagccta tccctaaccc tctcctcggt
       61 ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca
      121 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
      181 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
      241 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
      301 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
      361 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
      421 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      481 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      541 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc
      601 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
      661 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
      721 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc
      781 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
      841 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc
      901 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      961 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     1021 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     1081 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     1141 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     1201 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     1261 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
     1321 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     1381 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     1441 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     1501 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     1561 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     1621 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     1681 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     1741 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     1801 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1861 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     1921 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     1981 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     2041 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     2101 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     2161 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2221 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2281 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2341 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2401 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2461 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2521 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     2581 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2641 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2701 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2761 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     2821 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2881 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2941 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3001 cacgttaagg gattttggtc atgagcggat acatatttga atgtatttag aaaaataaac
     3061 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     3121 atctcccgat cccctatggt cgactctcag tacaatctgc tctgatgccg catagttaag
     3181 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     3241 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     3301 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     3361 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     3421 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     3481 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     3541 ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     3601 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     3661 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     3721 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     3781 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     3841 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     3901 gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
     3961 tatcgaaatt aatacgactc actataggga gacccaagct ggctagttaa gctcgccctt
//

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