| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-COL1A2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001672 |
| 产品用途: | 3' AAVLINK plasmid for COL1A2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | COL1A2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-COL1A2-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-COL1A2-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-COL1A2-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-COL1A2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-COL1A2-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-COL1A2-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-COL1A2-FLAG 7083 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7083)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7083
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3796
/codon_start=1
/label=COL1A2
/translation="VRVVLPVLLVLLEAEVLLDPQGLMETRVNLVWLVLWALLVHLVLV
DSQERGVLLAYLEAREKRVNLVSEVKLVTLAEMVLVVLLVL*VPLVLLEPQVTGAKLGL
LVLLVLLVLGEALVNVVRSVLLAPMDLLVLLVLLVNLVLKEKEEPKGLRVKTVLLVPQA
PLELLAQLVQMVPPVLLEVVVMEAPLV*LVSLVLLDGLVPQDPLVFLALLVPLVLLGKK
GFVVLVVTKVQLAELEK*VQLVPLASLVRRVPLERLVLLDLLALQVLRVFLVLLVFWVS
LAREVNVVYQVLLVLWVNLVLLALPALLGPVVLLVLWVVLESTVLLVKLVVMATLGTMV
PQVAMVNPDTRESAVTLAILVPLVLQVHLVLMAPWVLLANMETVVKLVLLVLLVLLVLL
AQEVLVAHKAFVAIRESPVKRGPEVFLA*RDTMDCKVCLVSLVTMVIKVLLAPWVLLVL
GALLVLLALLEKMVALDILVQLDLLAFEALRVTKALLAPLVPLALLDLQV*AVVVMTLV
TMETSTGLTSLAQHLLSDPRTMKLMLL*SLSTTRLRPFLLLKALERTQLAHAVT*DSAT
QSGAVVTTGLTLTKDALWMLSKYTVISLLAKPVSGPNLKTSQPRTGIGAPRTRNTSG*E
KLSMLAASLNIM*KE*LPRKWLPNLPSCACWPTMPLRTSPTTARTALHTWMRRLAT*KR
LSFYRALMMLNLLLRATAGSLTLFL*MAALKRQMNGERQSLNTKQISHHACPSLILHLW
TSVVLTRNSLWTLAQSVS"
misc_feature 2114..2119
/label=SDS
misc_feature 2267..2272
/label=SDS
misc_feature 2402..2407
/label=SDS
misc_feature 2420..2425
/label=SDS
misc_feature 2987..2992
/label=SDS
CDS 3803..3868
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3872..4303
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4016..4021
/label=SDS
repeat_region 4346..4486
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4346..4475
/label=AAV2 ITR
rep_origin 4561..5016
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5298..5402
/gene="bla"
/label=AmpR promoter
CDS 5403..6263
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6434..7022
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtgagagtg gtgctgccgg
1501 tcctactggt cctattggaa gccgaggtcc ttctggaccc ccagggcctg atggaaacaa
1561 gggtgaacct ggtgtggttg gtgctgtggg cactgctggt ccatctggtc ctagtggact
1621 cccaggagag aggggtgctg ctggcatacc tggaggcaag ggagaaaagg gtgaacctgg
1681 tctcagaggt gaaattggta accctggcag agatggtgct cgtggtgctc ctggtgctgt
1741 aggtgcccct ggtcctgctg gagccacagg tgaccggggc gaagctgggg ctgctggtcc
1801 tgctggtcct gctggtcctc ggggaagccc tggtgaacgt ggtgaggtcg gtcctgctgg
1861 ccccaatgga tttgctggtc ctgctggtgc tgctggtcaa cctggtgcta aaggagaaag
1921 aggagccaaa gggcctaagg gtgaaaacgg tgttgttggt cccacaggcc ccgttggagc
1981 tgctggccca gctggtccaa atggtccccc cggtcctgct ggaagtcgtg gtgatggagg
2041 cccccctggt atgactggtt tccctggtgc tgctggacgg actggtcccc caggaccctc
2101 tggtatttct ggccctcctg gtccccctgg tcctgctggg aaagaagggc ttcgtggtcc
2161 tcgtggtgac caaggtccag ttggccgaac tggagaagta ggtgcagttg gtccccctgg
2221 cttcgctggt gagaagggtc cctctggaga ggctggtact gctggacctc ctggcactcc
2281 aggtcctcag ggtcttcttg gtgctcctgg tattctgggt ctccctggct cgagaggtga
2341 acgtggtcta ccaggtgttg ctggtgctgt gggtgaacct ggtcctcttg gcattgccgg
2401 ccctcctggg gcccgtggtc ctcctggtgc tgtgggtagt cctggagtca acggtgctcc
2461 tggtgaagct ggtcgtgatg gcaaccctgg gaacgatggt cccccaggtc gcgatggtca
2521 acccggacac aagggagagc gcggttaccc tggcaatatt ggtcccgttg gtgctgcagg
2581 tgcacctggt cctcatggcc ccgtgggtcc tgctggcaaa catggaaacc gtggtgaaac
2641 tggtccttct ggtcctgttg gtcctgctgg tgctgttggc ccaagaggtc ctagtggccc
2701 acaaggcatt cgtggcgata agggagagcc cggtgaaaag gggcccagag gtcttcctgg
2761 cttaaaggga cacaatggat tgcaaggtct gcctggtatc gctggtcacc atggtgatca
2821 aggtgctcct ggctccgtgg gtcctgctgg tcctaggggc cctgctggtc cttctggccc
2881 tgctggaaaa gatggtcgca ctggacatcc tggtacagtt ggacctgctg gcattcgagg
2941 ccctcagggt caccaaggcc ctgctggccc ccctggtccc cctggccctc ctggacctcc
3001 aggtgtaagc ggtggtggtt atgactttgg ttacgatgga gacttctaca gggctgacca
3061 gcctcgctca gcaccttctc tcagacccaa ggactatgaa gttgatgcta ctctgaagtc
3121 tctcaacaac cagattgaga cccttcttac tcctgaaggc tctagaaaga acccagctcg
3181 cacatgccgt gacttgagac tcagccaccc agagtggagc agtggttact actggattga
3241 ccctaaccaa ggatgcacta tggatgctat caaagtatac tgtgatttct ctactggcga
3301 aacctgtatc cgggcccaac ctgaaaacat cccagccaag aactggtata ggagctccaa
3361 ggacaagaaa cacgtctggc taggagaaac tatcaatgct ggcagccagt ttgaatataa
3421 tgtagaagga gtgacttcca aggaaatggc tacccaactt gccttcatgc gcctgctggc
3481 caactatgcc tctcagaaca tcacctacca ctgcaagaac agcattgcat acatggatga
3541 ggagactggc aacctgaaaa aggctgtcat tctacagggc tctaatgatg ttgaacttgt
3601 tgctgagggc aacagcaggt tcacttacac tgttcttgta gatggctgct ctaaaaagac
3661 aaatgaatgg ggaaagacaa tcattgaata caaaacaaat aagccatcac gcctgccctt
3721 ccttgatatt gcacctttgg acatcggtgg tgctgaccag gaattctttg tggacattgg
3781 cccagtctgt ttcaaatgta cagactacaa agaccatgac ggtgattata aagatcatga
3841 catcgactac aaggatgacg atgacaagta actcgagata atcaacctct ggattacaaa
3901 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
3961 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
4021 ttgtataaat cctggttagt tcttgccacg gcggaactca tcgccgcctg ccttgcccgc
4081 tgctggacag gggctcggct gttgggcact gacaattccg tggtgtttat ttgtgaaatt
4141 tgtgatgcta ttgctttatt tgtaaccatc tagctttatt tgtgaaattt gtgatgctat
4201 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
4261 ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagatctg gagaccggtg
4321 tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct
4381 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
4441 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg
4501 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
4561 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
4621 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
4681 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
4741 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
4801 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
4861 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat
4921 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
4981 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
5041 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
5101 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
5161 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
5221 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
5281 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
5341 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
5401 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
5461 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
5521 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
5581 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
5641 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
5701 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
5761 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
5821 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
5881 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
5941 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
6001 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
6061 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
6121 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
6181 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
6241 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
6301 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
6361 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
6421 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
6481 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
6541 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
6601 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
6661 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
6721 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
6781 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
6841 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
6901 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
6961 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
7021 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
7081 tgt
//
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