| 质粒名称: | mCherry-TOMM20-N-10 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 55146 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | mCherry-N1 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4750 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | TOMM20,NM_014765.2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | mCherry（主干上的C终端） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | mCherry-TOMM20-N-10 was a gift from Michael Davidson (Addgene plasmid # 55146 ; http://n2t.net/addgene:55146 ; RRID:Addgene_55146) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS mCherry-TOMM20-N-10 5113 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Localization: Outer Mitochondrial Membrane, Excitation: 587,
Emission: 610.
ACCESSION .
VERSION .
KEYWORDS mCherry-TOMM20-N-10
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5113)
TITLE Davidson lab - mCherry constructs
REFERENCE 2 (bases 1 to 5113)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5113
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..304
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 459..479
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 537..546
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
regulatory 1002..1011
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1008..1718
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(1153..1171)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 1421..1440
/label=mCherry-F
/note="mCherry, forward primer"
polyA_signal 1841..1962
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1878..1897)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1932..1951
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1969..2424)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2106..2127)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2318..2337
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2451..2555
/gene="bla"
/label=AmpR promoter
promoter 2557..2914
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2580..2600)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2765..2900
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2827..2846
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2949..3743
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3003..3022)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3613..3632
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3931..3950)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3975..4022
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4351..4939
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4840..4859
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
541 ccatggtggg tcggaacagc gccatcgccg ccggtgtatg cggggccctt ttcattgggt
601 actgcatcta cttcgaccgc aaaagacgaa gtgaccccaa cttcaagaac aggcttcgag
661 aacgaagaaa gaaacagaag cttgccaagg agagagctgg gctttccaag ttacctgacc
721 ttaaagatgc tgaagctgtt cagaagttct tccttgaaga aatacagctt ggtgaagagt
781 tactagctca aggtgaatat gagaagggcg tagaccatct gacaaatgca attgctgtgt
841 gtggacagcc acagcagtta ctgcaggtct tacagcaaac tcttccacca ccagtgttcc
901 agatgcttct gactaagctc ccaacaatta gtcagagaat tgtaagtgct cagagcttgg
961 ctgaagatga tgtggaaggc ggtagcgggg atccaccggt cgccaccatg gtgagcaagg
1021 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
1081 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
1141 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
1201 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
1261 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
1321 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
1381 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
1441 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
1501 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
1561 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
1621 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
1681 gccactccac cggcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat
1741 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
1801 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
1861 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
1921 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg
1981 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
2041 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
2101 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
2161 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
2221 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
2281 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
2341 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
2401 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
2461 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
2521 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
2581 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
2641 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
2701 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
2761 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
2821 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
2881 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
2941 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
3001 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
3061 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
3121 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
3181 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
3241 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
3301 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
3361 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
3421 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
3481 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
3541 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
3601 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
3661 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
3721 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
3781 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
3841 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
3901 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
3961 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
4021 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
4081 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
4141 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
4201 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
4261 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
4321 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
4381 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
4441 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
4501 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
4561 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
4621 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
4681 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
4741 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
4801 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
4861 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
4921 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
4981 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
5041 taccgccatg cattagttat taatagtaat caattacggg gtcattagtt catagcccat
5101 atatggagtt ccg
//
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